FI91085B - Metod för bestämning och analysering av gram-negativa bakterier - Google Patents

Metod för bestämning och analysering av gram-negativa bakterier Download PDF

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Publication number
FI91085B
FI91085B FI910359A FI910359A FI91085B FI 91085 B FI91085 B FI 91085B FI 910359 A FI910359 A FI 910359A FI 910359 A FI910359 A FI 910359A FI 91085 B FI91085 B FI 91085B
Authority
FI
Finland
Prior art keywords
gram
bacteria
filter
sample
negative bacteria
Prior art date
Application number
FI910359A
Other languages
English (en)
Finnish (fi)
Other versions
FI910359A0 (sv
FI91085C (sv
FI910359A (sv
Inventor
Helena Marjatta Tuompo
Heljae Benita Glasin
Original Assignee
Orion Yhtymae Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orion Yhtymae Oy filed Critical Orion Yhtymae Oy
Publication of FI910359A0 publication Critical patent/FI910359A0/sv
Priority to FI910359A priority Critical patent/FI91085C/sv
Priority to US07/740,320 priority patent/US5420017A/en
Priority to AT92101132T priority patent/ATE144288T1/de
Priority to JP4032904A priority patent/JPH078292A/ja
Priority to DE69214473T priority patent/DE69214473T2/de
Priority to EP92101132A priority patent/EP0496409B1/en
Publication of FI910359A publication Critical patent/FI910359A/sv
Application granted granted Critical
Publication of FI91085B publication Critical patent/FI91085B/sv
Publication of FI91085C publication Critical patent/FI91085C/sv
Priority to US08/450,630 priority patent/US5714343A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/528—Atypical element structures, e.g. gloves, rods, tampons, toilet paper
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/20—Redox indicators
    • C12Q2304/24—Tetrazolium; Formazan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C12Q2326/92—Nitro blue tetrazolium chloride, i.e. NBT

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (8)

1. Hetod för pävisande av gram-negativa bakterier ur prov i vätskeform kännetecknad av att 5 a) mikroorganismerna urskiljs frän suspensionen medelst filtreringsmaterialet (3), vars porstorlek är sädan att mikroberna förhindras frän att genomtränga den vari-genom de koncentreras och optimal känslighet erhälles, 10 men ändä tillräckligt stor för att släppa igenom stö- rande fria reducerande föreningar, och b) pävisa närvaron av de gram-negativa bakteriernas dehydrogenasenzym pä filtret utan att sönderdela 15 organismerna genom att använda som dehydrogenasenzymets substrat sädana precipiterande redox-föreningar som förändras fr Iin färglös tili kolorerade direkt pä filtret där mikroorganismerna kvarblivit vid filtre-ringen, och 20 c) andra än gram-negativa bakteriers reducerande reaktion förhindras medelst kaotropiska eller ej-ioniserande detergent eller medelst en kombination av dessa. . 25
2. En metod enligt patentkrav 1, kännetecknad av att provet i vätskeform är urin eller processvätska.
3. En metod enligt patentkrav 2, kännetecknad 30 av att provet i vätskeform är urin.
4. En metod enligt patentkrav 1, kännetecknad av att redox-föreningen är precipiterande trifenyltet-ratsoliumklorid (TTC), jodonitrotetratsolium (INT), 35 neotetratsoliumklorid (NTC), blue tetratsolium (BT) eller nitrobluetetratsolium (NBT). 91085
5. En metod enligt patentkrav 1, kännetecknad av att filtrets (3) porstorlek är 0.75 - 1.2 μια.
6. En metod enligt patentkrav 1, kännetecknad 5 av att den kaotropiska föreningen är guanidin eller urea.
7. En metod enligt patentkrav 1, kännetecknad av att den ej-ioniserande alkylglukosidliknande deter- 10 genten är oktylglukosid eller oktylmaltosid.
8. En metod enligt patentkrav 1, kännetecknad av att det använda filtret är fäst till anordningen sälunda, att den begränsar sig tili den adsorberande 15 enheten som adsorberar överskotts vätskan samt de lösliga störande faktorerna. 20 25 30 35
FI910359A 1991-01-24 1991-01-24 Metod för bestämning och analysering av gram-negativa bakterier FI91085C (sv)

Priority Applications (7)

Application Number Priority Date Filing Date Title
FI910359A FI91085C (sv) 1991-01-24 1991-01-24 Metod för bestämning och analysering av gram-negativa bakterier
US07/740,320 US5420017A (en) 1991-01-24 1991-08-05 Method and kit for the detection of microorganisms
AT92101132T ATE144288T1 (de) 1991-01-24 1992-01-24 Ein verfahren zum nachweis gramnegativer bakterien
JP4032904A JPH078292A (ja) 1991-01-24 1992-01-24 グラム陰性細菌の検出方法
DE69214473T DE69214473T2 (de) 1991-01-24 1992-01-24 Ein Verfahren zum Nachweis gramnegativer Bakterien
EP92101132A EP0496409B1 (en) 1991-01-24 1992-01-24 A method for the detection of gram-negative bacteria
US08/450,630 US5714343A (en) 1991-01-24 1995-07-31 Method and kit for the detection of microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI910359A FI91085C (sv) 1991-01-24 1991-01-24 Metod för bestämning och analysering av gram-negativa bakterier
FI910359 1991-01-24

Publications (4)

Publication Number Publication Date
FI910359A0 FI910359A0 (sv) 1991-01-24
FI910359A FI910359A (sv) 1992-07-25
FI91085B true FI91085B (sv) 1994-01-31
FI91085C FI91085C (sv) 1994-05-10

Family

ID=8531792

Family Applications (1)

Application Number Title Priority Date Filing Date
FI910359A FI91085C (sv) 1991-01-24 1991-01-24 Metod för bestämning och analysering av gram-negativa bakterier

Country Status (5)

Country Link
EP (1) EP0496409B1 (sv)
JP (1) JPH078292A (sv)
AT (1) ATE144288T1 (sv)
DE (1) DE69214473T2 (sv)
FI (1) FI91085C (sv)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4220653A1 (de) * 1992-06-26 1994-01-13 Boehringer Mannheim Gmbh Verfahren und Mittel zum Nachweis eines Analyten enthaltend glykosidische Tenside
FR2732037B1 (fr) * 1995-03-20 1997-05-30 Dbv Procede de detection de microorganismes par separation et culture sur un systeme gelifie, systeme gelifie et necessaire de dosage pour mettre en oeuvre ce procede, utilisation en microbiologie
JP3825501B2 (ja) 1996-06-10 2006-09-27 吉郎 岡見 微小物質保持担体、その懸濁系、微小物質操作装置及び微小物質位置制御方法
US6790661B1 (en) 1999-07-16 2004-09-14 Verax Biomedical, Inc. System for detecting bacteria in blood, blood products, and fluids of tissues
US20110045470A1 (en) * 2007-08-01 2011-02-24 Hitachi Chemical Co., Ltd. Pathogen detection in large-volume particulate samples
US9598715B2 (en) * 2011-09-30 2017-03-21 Arkray, Inc. Method for measuring color change of oxidation-reduction indicator
JP6135700B2 (ja) 2015-03-11 2017-05-31 トヨタ自動車株式会社 車体前部構造
CN115461466A (zh) * 2020-05-12 2022-12-09 株式会社日立高新技术 自动分析装置、自动分析方法
CN112080547A (zh) * 2020-09-04 2020-12-15 斯微(上海)生物科技有限公司 一种快速的无菌检查方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4525453A (en) * 1982-10-26 1985-06-25 Eastman Kodak Company Process for rapidly differentiating between gram-negative and gram-positive microorganisms

Also Published As

Publication number Publication date
FI910359A0 (sv) 1991-01-24
FI91085C (sv) 1994-05-10
DE69214473T2 (de) 1997-05-22
EP0496409A1 (en) 1992-07-29
ATE144288T1 (de) 1996-11-15
DE69214473D1 (de) 1996-11-21
FI910359A (sv) 1992-07-25
JPH078292A (ja) 1995-01-13
EP0496409B1 (en) 1996-10-16

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FG Patent granted

Owner name: ORION-YHTYMAE OY

BB Publication of examined application