FI120266B - Öljyonteloproteiinit arvokkaiden peptidien kantajina kasveissa - Google Patents

Öljyonteloproteiinit arvokkaiden peptidien kantajina kasveissa Download PDF

Info

Publication number
FI120266B
FI120266B FI944841A FI944841A FI120266B FI 120266 B FI120266 B FI 120266B FI 944841 A FI944841 A FI 944841A FI 944841 A FI944841 A FI 944841A FI 120266 B FI120266 B FI 120266B
Authority
FI
Finland
Prior art keywords
protein
chimeric
peptide
dna
expression
Prior art date
Application number
FI944841A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI944841A (fi
FI944841A0 (fi
Inventor
Maurice M Moloney
Original Assignee
Sembiosys Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sembiosys Genetics Inc filed Critical Sembiosys Genetics Inc
Priority to FI944841A priority Critical patent/FI120266B/fi
Publication of FI944841A0 publication Critical patent/FI944841A0/fi
Publication of FI944841A publication Critical patent/FI944841A/fi
Application granted granted Critical
Publication of FI120266B publication Critical patent/FI120266B/fi

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormone [GH], i.e. somatotropin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8234Seed-specific, e.g. embryo, endosperm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6483Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23Aspartic endopeptidases (3.4.23)
    • C12Y304/23004Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Seasonings (AREA)
  • Edible Oils And Fats (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FI944841A 1992-04-15 1994-10-14 Öljyonteloproteiinit arvokkaiden peptidien kantajina kasveissa FI120266B (fi)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FI944841A FI120266B (fi) 1992-04-15 1994-10-14 Öljyonteloproteiinit arvokkaiden peptidien kantajina kasveissa

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
PCT/CA1992/000161 WO1993021320A1 (en) 1991-02-22 1992-04-15 Oil-body proteins as carriers of high-value peptides in plants
CA9200161 1992-04-15
FI944841A FI120266B (fi) 1992-04-15 1994-10-14 Öljyonteloproteiinit arvokkaiden peptidien kantajina kasveissa
FI944841 1994-10-14

Publications (3)

Publication Number Publication Date
FI944841A0 FI944841A0 (fi) 1994-10-14
FI944841A FI944841A (fi) 1994-12-14
FI120266B true FI120266B (fi) 2009-08-31

Family

ID=32778493

Family Applications (1)

Application Number Title Priority Date Filing Date
FI944841A FI120266B (fi) 1992-04-15 1994-10-14 Öljyonteloproteiinit arvokkaiden peptidien kantajina kasveissa

Country Status (11)

Country Link
EP (1) EP0636179B1 (de)
JP (1) JP3639592B2 (de)
KR (1) KR950700992A (de)
AT (1) ATE277180T1 (de)
CA (1) CA2118113C (de)
DE (1) DE69233416T2 (de)
DK (1) DK0636179T3 (de)
ES (1) ES2227511T3 (de)
FI (1) FI120266B (de)
NO (1) NO323038B1 (de)
WO (1) WO1993021320A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650554A (en) * 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
US7091401B2 (en) 1991-02-22 2006-08-15 Sembiosys Genetics Inc. Expression of epidermal growth factor in plant seeds
US5869602A (en) 1995-03-17 1999-02-09 Novo Nordisk A/S Peptide derivatives
US5824870A (en) * 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
WO1998021348A1 (en) * 1996-11-12 1998-05-22 Battelle Memorial Institute Method of producing human growth factors from whole plants or plant cell cultures
US5856452A (en) 1996-12-16 1999-01-05 Sembiosys Genetics Inc. Oil bodies and associated proteins as affinity matrices
FR2757874B1 (fr) * 1996-12-17 2003-04-25 Biocem Collagenes recombinants et proteines derivees produits par les plantes, leurs procedes d'obtention et leurs utilisations
US7585645B2 (en) * 1997-05-27 2009-09-08 Sembiosys Genetics Inc. Thioredoxin and thioredoxin reductase containing oil body based products
US7008664B1 (en) * 1998-06-11 2006-03-07 E. I. Du Pont De Nemours And Company Method for improving the carcass quality of an animal
GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
WO2004113540A1 (en) * 2003-06-18 2004-12-29 The University Of York Expression of heterologous protein
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
WO2007045019A2 (en) 2005-10-19 2007-04-26 Agriculture Victoria Services Pty Ltd Polyoleosins
EP2044207A1 (de) * 2006-07-11 2009-04-08 Basf Se Steuern von proteinen auf lipidkörper als ziel
CN114409729B (zh) * 2021-11-11 2023-06-20 南京财经大学 一种菜籽肽及其在制备药物纳米载体方面的应用

Also Published As

Publication number Publication date
NO943914D0 (no) 1994-10-14
WO1993021320A1 (en) 1993-10-28
FI944841A (fi) 1994-12-14
NO323038B1 (no) 2006-12-27
DK0636179T3 (da) 2004-12-20
AU678136B2 (en) 1997-05-22
KR950700992A (ko) 1995-02-20
CA2118113C (en) 2002-01-08
ES2227511T3 (es) 2005-04-01
EP0636179A1 (de) 1995-02-01
DE69233416T2 (de) 2005-03-03
DE69233416D1 (de) 2004-10-28
FI944841A0 (fi) 1994-10-14
ATE277180T1 (de) 2004-10-15
CA2118113A1 (en) 1993-10-28
AU1583292A (en) 1993-11-18
EP0636179B1 (de) 2004-09-22
NO943914L (no) 1994-12-09
JP3639592B2 (ja) 2005-04-20
JPH07507199A (ja) 1995-08-10

Similar Documents

Publication Publication Date Title
FI120266B (fi) Öljyonteloproteiinit arvokkaiden peptidien kantajina kasveissa
US5650554A (en) Oil-body proteins as carriers of high-value peptides in plants
US5948682A (en) Preparation of heterologous proteins on oil bodies
US5487991A (en) Process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
FI120314B (fi) Öljyonteloproteiini-cis-elementit säätelysignaaleina
JP3232068B2 (ja) 遺伝子導入植物内の修飾された貯蔵種子タンパク質遺伝子の発現による生物学的に活性なペプチドの製造方法
US6753167B2 (en) Preparation of heterologous proteins on oil bodies
Saalbach et al. A chimeric gene encoding the methionine-rich 2S albumin of the Brazil nut (Bertlrolletia excelsa HBK) is stably expressed and inherited in transgenic grain legumes
US6777591B1 (en) Legume-like storage protein promoter isolated from flax and methods of expressing proteins in plant seeds using the promoter
ZA200103557B (en) Novel expression cassette for expressing genes in plant seed.
US6288304B1 (en) Expression of somatotropin in plant seeds
KR20050024419A (ko) 식물 소포체 파생적 단백질체들 내의 축적에 의한 펩티드및 단백질들의 생산
CA1337048C (en) Process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
JPH04229182A (ja) 新規シグナル配列
US5670635A (en) Seed storage protein with nutritionally balanced amino acid composition
EP0319353B1 (de) Verfahren zur Herstellung eines biologisch-aktiven Peptides durch Expression von modifizierten Speicherproteingenen in transgenetischen Pflanzen
RU2201449C2 (ru) Слитый полипептид, способный к целенаправленному переносу к масляному телу, химерная днк-контрукция, экспрессирующая кассета
AU678136C (en) Oil-body proteins as carriers of high-value peptides in plants
JP3927975B2 (ja) 植物中の高価値ペプチドの担体用の油体タンパク質
US20040117874A1 (en) Methods for accumulating translocated proteins
EP0723019A1 (de) Verfahren zur Herstellung eines biologisch-aktiven Peptides durch Expression von modifizierten Speicherproteingenen in transgenen Pflanzen
KR100333315B1 (ko) 식물에서 가치있는 펩타이드의 운반체인 기름체 단백질

Legal Events

Date Code Title Description
GB Transfer or assigment of application

Owner name: SEMBIOSYS GENETICS INC.

FG Patent granted

Ref document number: 120266

Country of ref document: FI

MM Patent lapsed