DK0636179T3 - Olielegemeproteiner som bærestoffer af höjværdi-peptider i planter - Google Patents

Olielegemeproteiner som bærestoffer af höjværdi-peptider i planter

Info

Publication number
DK0636179T3
DK0636179T3 DK92908088T DK92908088T DK0636179T3 DK 0636179 T3 DK0636179 T3 DK 0636179T3 DK 92908088 T DK92908088 T DK 92908088T DK 92908088 T DK92908088 T DK 92908088T DK 0636179 T3 DK0636179 T3 DK 0636179T3
Authority
DK
Denmark
Prior art keywords
oil body
fusion protein
protein
polypeptide
interest
Prior art date
Application number
DK92908088T
Other languages
Danish (da)
English (en)
Inventor
Maurice M Moloney
Original Assignee
Sembiosys Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sembiosys Genetics Inc filed Critical Sembiosys Genetics Inc
Application granted granted Critical
Publication of DK0636179T3 publication Critical patent/DK0636179T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Seasonings (AREA)
  • Edible Oils And Fats (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK92908088T 1992-04-15 1992-04-15 Olielegemeproteiner som bærestoffer af höjværdi-peptider i planter DK0636179T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CA1992/000161 WO1993021320A1 (en) 1991-02-22 1992-04-15 Oil-body proteins as carriers of high-value peptides in plants

Publications (1)

Publication Number Publication Date
DK0636179T3 true DK0636179T3 (da) 2004-12-20

Family

ID=32778493

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92908088T DK0636179T3 (da) 1992-04-15 1992-04-15 Olielegemeproteiner som bærestoffer af höjværdi-peptider i planter

Country Status (11)

Country Link
EP (1) EP0636179B1 (de)
JP (1) JP3639592B2 (de)
KR (1) KR950700992A (de)
AT (1) ATE277180T1 (de)
CA (1) CA2118113C (de)
DE (1) DE69233416T2 (de)
DK (1) DK0636179T3 (de)
ES (1) ES2227511T3 (de)
FI (1) FI120266B (de)
NO (1) NO323038B1 (de)
WO (1) WO1993021320A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650554A (en) * 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
US7091401B2 (en) 1991-02-22 2006-08-15 Sembiosys Genetics Inc. Expression of epidermal growth factor in plant seeds
US5869602A (en) * 1995-03-17 1999-02-09 Novo Nordisk A/S Peptide derivatives
US5824870A (en) * 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
WO1998021348A1 (en) * 1996-11-12 1998-05-22 Battelle Memorial Institute Method of producing human growth factors from whole plants or plant cell cultures
US5856452A (en) * 1996-12-16 1999-01-05 Sembiosys Genetics Inc. Oil bodies and associated proteins as affinity matrices
FR2757874B1 (fr) * 1996-12-17 2003-04-25 Biocem Collagenes recombinants et proteines derivees produits par les plantes, leurs procedes d'obtention et leurs utilisations
US7585645B2 (en) * 1997-05-27 2009-09-08 Sembiosys Genetics Inc. Thioredoxin and thioredoxin reductase containing oil body based products
US7008664B1 (en) 1998-06-11 2006-03-07 E. I. Du Pont De Nemours And Company Method for improving the carcass quality of an animal
GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
WO2004113540A1 (en) * 2003-06-18 2004-12-29 The University Of York Expression of heterologous protein
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
NZ567603A (en) * 2005-10-19 2012-03-30 Agriculture Victoria Serv Pty Constructs including one or more nucleic acids encoding between three and twenty oleosin repeat units
JP2009542240A (ja) * 2006-07-11 2009-12-03 ビーエーエスエフ ソシエタス・ヨーロピア 脂質体へのタンパク質ターゲティング
CN114409729B (zh) * 2021-11-11 2023-06-20 南京财经大学 一种菜籽肽及其在制备药物纳米载体方面的应用

Also Published As

Publication number Publication date
JPH07507199A (ja) 1995-08-10
DE69233416D1 (de) 2004-10-28
AU678136B2 (en) 1997-05-22
CA2118113C (en) 2002-01-08
AU1583292A (en) 1993-11-18
DE69233416T2 (de) 2005-03-03
FI944841A (fi) 1994-12-14
JP3639592B2 (ja) 2005-04-20
NO943914L (no) 1994-12-09
EP0636179B1 (de) 2004-09-22
KR950700992A (ko) 1995-02-20
EP0636179A1 (de) 1995-02-01
ES2227511T3 (es) 2005-04-01
FI120266B (fi) 2009-08-31
CA2118113A1 (en) 1993-10-28
FI944841A0 (fi) 1994-10-14
NO323038B1 (no) 2006-12-27
NO943914D0 (no) 1994-10-14
ATE277180T1 (de) 2004-10-15
WO1993021320A1 (en) 1993-10-28

Similar Documents

Publication Publication Date Title
DK0636179T3 (da) Olielegemeproteiner som bærestoffer af höjværdi-peptider i planter
AR000610A1 (es) Un método para la expresión de un polipéptido recombinante por una célula huésped
Tenenhouse et al. The isolation and characterization of thyrocalcitonin
DE112006000677A5 (de) Rekombinante Expression von Proteinen in einer disulfidverbrückten, zweikettigen Form
ES2114539T3 (es) Expresion de polipeptidos recombinados con purificacion mejorada.
DE69227041D1 (de) Expression und reinigung von rekombinantem, löslichem gewebefaktor
IL152886A0 (en) B-7 related nucleic acids and polypeptides and pharmaceutical compositions containing the same
EA199900575A1 (ru) Композиции на основе слитого белка ов и способы их применения
AR058982A1 (es) Vector de expresion de la proteina bicunina y metodo para producir dicha proteina
IE883612L (en) Vectors and compounds for direct expression of activated human protein c
Travis et al. The amino acid sequence of ferredoxin from the sulfate reducing bacterium, Desulfovibrio gigas
ES2129487T3 (es) Peptidos sinteticos, anticuerpos dirigidos contra ellos y su utilizacion.
DK430486A (da) Fremgangsmaade til udvinding af protein
Nakai et al. Amino acid sequence of toxin A from the venom of the Indian cobra (Naja naja)
DE68927379T2 (de) Plättchen blockierende peptide
WO2005056578A3 (en) High-level expression of fusion polypeptides in plant seeds utilizing seed-storage proteins as fusion carriers
NO930428D0 (no) Proteinstrukturen til plantetoksingelonin
EP0369816A3 (de) Gegen menschliches polymorphes epitheliales Muzin spezifische monoklonale Antikörper
NO900758D0 (no) Fremgangsmaate for fremstilling av hybridprotein.
ITRM920658A1 (it) Procedimento per la purificazione della proteina big endotelina
Langlais et al. The phospholipase A2 of human spermatozoa; purification and partial sequence
DE3853383D1 (de) Isolierung von einkettigen proteinen mit fsh unter- drückender aktivität aus follikularflüssigkeit.
DE3873273D1 (de) Verfahren zur selektiven spaltung von fusionsproteinen.
RU94046181A (ru) Слитые полипептиды, химерная днк, экспрессионные кассеты, способы осуществления экспрессии, способ получения очищенного пептида, клетки растения, растения, семена, способ получения пептида
Mizuta et al. Subunit composition of two distinct types of collagen in the muscle of the squid Todarodes pacificus