FI114318B - Menetelmä rekombinanttiadenovirusrokotteiden valmistamiseksi - Google Patents
Menetelmä rekombinanttiadenovirusrokotteiden valmistamiseksi Download PDFInfo
- Publication number
- FI114318B FI114318B FI933495A FI933495A FI114318B FI 114318 B FI114318 B FI 114318B FI 933495 A FI933495 A FI 933495A FI 933495 A FI933495 A FI 933495A FI 114318 B FI114318 B FI 114318B
- Authority
- FI
- Finland
- Prior art keywords
- gag
- hiv
- adenovirus
- recombinant
- tplhrev
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 15
- 238000002360 preparation method Methods 0.000 title description 13
- 229940021704 adenovirus vaccine Drugs 0.000 title description 4
- 230000008569 process Effects 0.000 title description 2
- 241000701161 unidentified adenovirus Species 0.000 claims abstract description 68
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 25
- 229960005486 vaccine Drugs 0.000 claims abstract description 25
- 239000000427 antigen Substances 0.000 claims abstract description 20
- 108091007433 antigens Proteins 0.000 claims abstract description 20
- 102000036639 antigens Human genes 0.000 claims abstract description 20
- 230000024932 T cell mediated immunity Effects 0.000 claims abstract description 10
- 241000124008 Mammalia Species 0.000 claims abstract description 7
- 238000004519 manufacturing process Methods 0.000 claims abstract description 7
- 210000002845 virion Anatomy 0.000 claims abstract description 3
- 230000001939 inductive effect Effects 0.000 claims abstract 2
- 241000725303 Human immunodeficiency virus Species 0.000 claims description 37
- 241000713772 Human immunodeficiency virus 1 Species 0.000 claims description 17
- 210000003928 nasal cavity Anatomy 0.000 claims description 11
- 108700004026 gag Genes Proteins 0.000 claims description 8
- 108700004030 rev Genes Proteins 0.000 claims description 7
- 108020004705 Codon Proteins 0.000 claims description 6
- 108090000288 Glycoproteins Proteins 0.000 claims description 6
- 102000003886 Glycoproteins Human genes 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 101150098213 rev gene Proteins 0.000 claims description 6
- 230000036039 immunity Effects 0.000 claims description 5
- 241000598171 Human adenovirus sp. Species 0.000 claims description 4
- 238000003780 insertion Methods 0.000 claims description 4
- 230000037431 insertion Effects 0.000 claims description 4
- 108700005075 Regulator Genes Proteins 0.000 claims description 3
- 230000010076 replication Effects 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 2
- 101150059999 pro gene Proteins 0.000 claims description 2
- 238000011144 upstream manufacturing Methods 0.000 claims description 2
- 239000003086 colorant Substances 0.000 claims 2
- RYXPMWYHEBGTRV-UHFFFAOYSA-N Omeprazole sodium Chemical compound [Na+].N=1C2=CC(OC)=CC=C2[N-]C=1S(=O)CC1=NC=C(C)C(OC)=C1C RYXPMWYHEBGTRV-UHFFFAOYSA-N 0.000 claims 1
- WDVSHHCDHLJJJR-UHFFFAOYSA-N Proflavine Chemical compound C1=CC(N)=CC2=NC3=CC(N)=CC=C3C=C21 WDVSHHCDHLJJJR-UHFFFAOYSA-N 0.000 claims 1
- MKUXAQIIEYXACX-UHFFFAOYSA-N aciclovir Chemical compound N1C(N)=NC(=O)C2=C1N(COCCO)C=N2 MKUXAQIIEYXACX-UHFFFAOYSA-N 0.000 claims 1
- 239000010445 mica Substances 0.000 claims 1
- 229910052618 mica group Inorganic materials 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 238000001228 spectrum Methods 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 abstract description 18
- 230000002458 infectious effect Effects 0.000 abstract description 6
- 101710172711 Structural protein Proteins 0.000 abstract description 2
- 241000700605 Viruses Species 0.000 description 51
- 241000282579 Pan Species 0.000 description 37
- 210000004027 cell Anatomy 0.000 description 35
- 241001465754 Metazoa Species 0.000 description 24
- 241000282577 Pan troglodytes Species 0.000 description 18
- 208000031886 HIV Infections Diseases 0.000 description 15
- 230000003053 immunization Effects 0.000 description 14
- 238000002649 immunization Methods 0.000 description 14
- 238000006386 neutralization reaction Methods 0.000 description 13
- 210000002966 serum Anatomy 0.000 description 13
- 230000036436 anti-hiv Effects 0.000 description 12
- 230000004044 response Effects 0.000 description 12
- 230000003472 neutralizing effect Effects 0.000 description 10
- 238000002255 vaccination Methods 0.000 description 10
- 230000003612 virological effect Effects 0.000 description 10
- 238000001262 western blot Methods 0.000 description 10
- 239000012634 fragment Substances 0.000 description 9
- 239000002775 capsule Substances 0.000 description 8
- 230000028327 secretion Effects 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 210000003608 fece Anatomy 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 230000000968 intestinal effect Effects 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 5
- 238000002965 ELISA Methods 0.000 description 5
- 229940037003 alum Drugs 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 208000030507 AIDS Diseases 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 230000005875 antibody response Effects 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 101150098622 gag gene Proteins 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 230000003248 secreting effect Effects 0.000 description 4
- 108091026890 Coding region Proteins 0.000 description 3
- 101710177291 Gag polyprotein Proteins 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 108010078428 env Gene Products Proteins 0.000 description 3
- 230000002550 fecal effect Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 230000028996 humoral immune response Effects 0.000 description 3
- 230000005847 immunogenicity Effects 0.000 description 3
- 230000021633 leukocyte mediated immunity Effects 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 229920001220 nitrocellulos Polymers 0.000 description 3
- 210000003296 saliva Anatomy 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241001200329 Chanda Species 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 108700020121 Human Immunodeficiency Virus-1 rev Proteins 0.000 description 2
- 241000701096 Human adenovirus 7 Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000700618 Vaccinia virus Species 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- GAMPNQJDUFQVQO-UHFFFAOYSA-N acetic acid;phthalic acid Chemical compound CC(O)=O.OC(=O)C1=CC=CC=C1C(O)=O GAMPNQJDUFQVQO-UHFFFAOYSA-N 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000003263 anti-adenoviral effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000013480 data collection Methods 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 108700004025 env Genes Proteins 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 230000002163 immunogen Effects 0.000 description 2
- 238000010255 intramuscular injection Methods 0.000 description 2
- 239000007927 intramuscular injection Substances 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 2
- 210000001331 nose Anatomy 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 201000001178 Bacterial Pneumonia Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241001609030 Brosme brosme Species 0.000 description 1
- 210000004366 CD4-positive T-lymphocyte Anatomy 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 231100000023 Cell-mediated cytotoxicity Toxicity 0.000 description 1
- 206010057250 Cell-mediated cytotoxicity Diseases 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 101710091045 Envelope protein Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 229940033330 HIV vaccine Drugs 0.000 description 1
- MSPSOUKLPSVQMY-UHFFFAOYSA-N Hirtin Natural products CC12C(OC(=O)CC)C(OC(C)=O)C3C4(C)C=CC(=O)C(C)(C(=O)OC)C4=C(O)C(=O)C3(C)C32OC3CC1C=1C=COC=1 MSPSOUKLPSVQMY-UHFFFAOYSA-N 0.000 description 1
- 108010065155 Human Immunodeficiency Virus env Gene Products Proteins 0.000 description 1
- 102100034349 Integrase Human genes 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 241000282553 Macaca Species 0.000 description 1
- 101710125418 Major capsid protein Proteins 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- 101710188315 Protein X Proteins 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000193998 Streptococcus pneumoniae Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000534944 Thia Species 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000011888 autopsy Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008512 biological response Effects 0.000 description 1
- 230000035584 blastogenesis Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000005890 cell-mediated cytotoxicity Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 210000003756 cervix mucus Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 101150030339 env gene Proteins 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 239000007903 gelatin capsule Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- JFCQEDHGNNZCLN-UHFFFAOYSA-N glutaric acid Chemical compound OC(=O)CCCC(O)=O JFCQEDHGNNZCLN-UHFFFAOYSA-N 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 210000003563 lymphoid tissue Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229940111688 monobasic potassium phosphate Drugs 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 210000001989 nasopharynx Anatomy 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 229940126578 oral vaccine Drugs 0.000 description 1
- 229910052763 palladium Inorganic materials 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- -1 phosphate-10 Chemical compound 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002594 sorbent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 229940031000 streptococcus pneumoniae Drugs 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 229940125575 vaccine candidate Drugs 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
- C12N2740/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Biophysics (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- AIDS & HIV (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US92649192A | 1992-08-07 | 1992-08-07 | |
| US92649192 | 1992-08-07 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI933495A0 FI933495A0 (fi) | 1993-08-06 |
| FI933495A FI933495A (fi) | 1994-02-08 |
| FI114318B true FI114318B (fi) | 2004-09-30 |
Family
ID=25453282
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI933495A FI114318B (fi) | 1992-08-07 | 1993-08-06 | Menetelmä rekombinanttiadenovirusrokotteiden valmistamiseksi |
Country Status (18)
| Country | Link |
|---|---|
| EP (1) | EP0586076B1 (fr) |
| JP (1) | JP4093597B2 (fr) |
| KR (1) | KR100347219B1 (fr) |
| AT (1) | ATE243755T1 (fr) |
| AU (2) | AU680826B2 (fr) |
| BR (1) | BR9303226A (fr) |
| CA (1) | CA2101463C (fr) |
| DE (1) | DE69333057T2 (fr) |
| DK (1) | DK0586076T3 (fr) |
| ES (1) | ES2201055T3 (fr) |
| FI (1) | FI114318B (fr) |
| HK (1) | HK1009978A1 (fr) |
| HU (2) | HU214364B (fr) |
| IL (1) | IL106508A (fr) |
| MX (1) | MX9304765A (fr) |
| NZ (1) | NZ248328A (fr) |
| PT (1) | PT586076E (fr) |
| ZA (1) | ZA935355B (fr) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2196018T3 (es) * | 1993-08-11 | 2003-12-16 | Wyeth Corp | Vacunas de adenovirus recombinantes. |
| IL113817A (en) * | 1994-06-30 | 2001-03-19 | Merck & Co Inc | Polynucleotide for vaccination against the umbilical cord virus |
| FR2727867B1 (fr) * | 1994-12-13 | 1997-01-31 | Rhone Poulenc Rorer Sa | Transfert de genes dans les motoneurones medullaires au moyen de vecteurs adenoviraux |
| JPH10512243A (ja) * | 1994-12-30 | 1998-11-24 | カイロン コーポレイション | 遺伝子送達ビヒクルの非外傷性投与 |
| KR100464395B1 (ko) * | 1997-10-13 | 2005-02-28 | 삼성전자주식회사 | 반도체소자의비아홀형성방법 |
| EP1200622A4 (fr) | 1999-07-06 | 2004-12-22 | Merck & Co Inc | Vaccin contre le vih comprenant un adenovirus vehiculant le gene gag |
| US6733993B2 (en) | 2000-09-15 | 2004-05-11 | Merck & Co., Inc. | Enhanced first generation adenovirus vaccines expressing codon optimized HIV1-gag, pol, nef and modifications |
| US8926987B2 (en) | 2004-11-18 | 2015-01-06 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Replication-competent adenoviral vectors |
| US9132031B2 (en) | 2006-09-26 | 2015-09-15 | Zeltiq Aesthetics, Inc. | Cooling device having a plurality of controllable cooling elements to provide a predetermined cooling profile |
| US20080287839A1 (en) | 2007-05-18 | 2008-11-20 | Juniper Medical, Inc. | Method of enhanced removal of heat from subcutaneous lipid-rich cells and treatment apparatus having an actuator |
| US8523927B2 (en) | 2007-07-13 | 2013-09-03 | Zeltiq Aesthetics, Inc. | System for treating lipid-rich regions |
| US8285390B2 (en) | 2007-08-21 | 2012-10-09 | Zeltiq Aesthetics, Inc. | Monitoring the cooling of subcutaneous lipid-rich cells, such as the cooling of adipose tissue |
| US8603073B2 (en) | 2008-12-17 | 2013-12-10 | Zeltiq Aesthetics, Inc. | Systems and methods with interrupt/resume capabilities for treating subcutaneous lipid-rich cells |
| EP2769703B1 (fr) | 2009-04-30 | 2022-04-06 | Zeltiq Aesthetics, Inc. | Dispositif d'élimination de chaleur à partir de cellules riches en lipide sous-cutanées |
| BR112012018521A2 (pt) | 2010-01-25 | 2019-09-24 | Zeltiq Aesthetics Inc | aplicadores para uso doméstico para remover de forma não invasiva calor de células subcutâneas ricas em lipídeos por meio de refrigerantes de mudança de fase, e dispositivos, sistemas e métodos associados |
| US8676338B2 (en) | 2010-07-20 | 2014-03-18 | Zeltiq Aesthetics, Inc. | Combined modality treatment systems, methods and apparatus for body contouring applications |
| US10722395B2 (en) | 2011-01-25 | 2020-07-28 | Zeltiq Aesthetics, Inc. | Devices, application systems and methods with localized heat flux zones for removing heat from subcutaneous lipid-rich cells |
| WO2020028472A1 (fr) | 2018-07-31 | 2020-02-06 | Zeltiq Aesthetics, Inc. | Procédés, dispositifs et systèmes pour l'amélioration d'une ou de plusieurs caractéristiques de la peau |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4925784A (en) * | 1986-04-04 | 1990-05-15 | Hoffmann-La Roche Inc. | Expression and purification of an HTLV-III gag/env gene protein |
| US5585264A (en) * | 1988-07-15 | 1996-12-17 | University Of Saskatchewan | Nucleotide sequences encoding recombinant bovine herpesvirus type-1 GI, GIII and GIV polypeptides |
| GB8919102D0 (en) * | 1989-08-22 | 1989-10-04 | Oxford Virology Ltd | A recombinant adenovirus dna sequence,a recombinant adenovirus expressing the dna sequence and a rhabdovirus vaccine including the recombinant adenovirus |
| AU9179891A (en) * | 1990-12-19 | 1992-07-22 | Epitope, Inc. | Hiv reverse transcriptase vaccine |
| GB9114437D0 (en) * | 1991-07-03 | 1991-08-21 | Health Lab Service Board | Recombinant adenoviruses and the use thereof for detecting the presence in eukaryotic cells of the expression products of specific genes |
-
1993
- 1993-07-23 EP EP93305833A patent/EP0586076B1/fr not_active Expired - Lifetime
- 1993-07-23 DE DE69333057T patent/DE69333057T2/de not_active Expired - Fee Related
- 1993-07-23 PT PT93305833T patent/PT586076E/pt unknown
- 1993-07-23 ES ES93305833T patent/ES2201055T3/es not_active Expired - Lifetime
- 1993-07-23 AT AT93305833T patent/ATE243755T1/de not_active IP Right Cessation
- 1993-07-23 DK DK93305833T patent/DK0586076T3/da active
- 1993-07-23 ZA ZA935355A patent/ZA935355B/xx unknown
- 1993-07-28 CA CA002101463A patent/CA2101463C/fr not_active Expired - Fee Related
- 1993-07-28 IL IL106508A patent/IL106508A/en not_active IP Right Cessation
- 1993-07-30 BR BR9303226A patent/BR9303226A/pt not_active Application Discontinuation
- 1993-08-04 NZ NZ248328A patent/NZ248328A/en not_active IP Right Cessation
- 1993-08-04 JP JP19341093A patent/JP4093597B2/ja not_active Expired - Fee Related
- 1993-08-05 MX MX9304765A patent/MX9304765A/es not_active IP Right Cessation
- 1993-08-06 KR KR1019930015236A patent/KR100347219B1/ko not_active IP Right Cessation
- 1993-08-06 FI FI933495A patent/FI114318B/fi active IP Right Grant
- 1993-08-06 AU AU44465/93A patent/AU680826B2/en not_active Ceased
- 1993-08-06 HU HU9302285A patent/HU214364B/hu not_active IP Right Cessation
-
1995
- 1995-06-30 HU HU95P/P00643P patent/HU211691A9/hu unknown
-
1997
- 1997-08-18 AU AU34227/97A patent/AU715190B2/en not_active Ceased
-
1998
- 1998-09-19 HK HK98110746A patent/HK1009978A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2101463A1 (fr) | 1994-02-08 |
| HK1009978A1 (en) | 1999-06-11 |
| HU211691A9 (en) | 1995-12-28 |
| KR100347219B1 (ko) | 2003-02-25 |
| IL106508A0 (en) | 1993-11-15 |
| FI933495A0 (fi) | 1993-08-06 |
| FI933495A (fi) | 1994-02-08 |
| DE69333057T2 (de) | 2004-07-08 |
| PT586076E (pt) | 2003-11-28 |
| EP0586076A3 (fr) | 1994-04-20 |
| IL106508A (en) | 1998-02-22 |
| CA2101463C (fr) | 2007-03-13 |
| EP0586076A2 (fr) | 1994-03-09 |
| DK0586076T3 (da) | 2003-10-20 |
| DE69333057D1 (de) | 2003-07-31 |
| MX9304765A (es) | 1995-01-31 |
| EP0586076B1 (fr) | 2003-06-25 |
| KR940003566A (ko) | 1994-03-12 |
| JPH06165689A (ja) | 1994-06-14 |
| ATE243755T1 (de) | 2003-07-15 |
| HU9302285D0 (en) | 1993-11-29 |
| ES2201055T3 (es) | 2004-03-16 |
| NZ248328A (en) | 1997-05-26 |
| HUT67302A (en) | 1995-03-28 |
| BR9303226A (pt) | 1994-03-08 |
| ZA935355B (en) | 1995-01-23 |
| AU715190B2 (en) | 2000-01-20 |
| AU3422797A (en) | 1997-11-20 |
| HU214364B (hu) | 1998-03-30 |
| AU680826B2 (en) | 1997-08-14 |
| AU4446593A (en) | 1994-02-10 |
| JP4093597B2 (ja) | 2008-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FI114318B (fi) | Menetelmä rekombinanttiadenovirusrokotteiden valmistamiseksi | |
| NATUK et al. | Immunogenicity of recombinant human adenovirus-human immunodeficiency virus vaccines in chimpanzees | |
| US6511845B1 (en) | Methods for producing an immune response against HIV-1 | |
| US5614404A (en) | Self-assembled, defective, non-self-propagating lentivirus particles | |
| CH676247A5 (fr) | ||
| WO1991019803A1 (fr) | Particules virales autoassemblees defectueuses qui ne se propagent pas d'elles-memes | |
| US6051410A (en) | Self-assembled, defective, nonself-propagating viral particles | |
| JP3034025B2 (ja) | 自己アセンブルド欠損非自己増殖性ウイルス粒子 | |
| EP0638316B1 (fr) | Vaccins à adénovirus recombinant | |
| JPH10512151A (ja) | Ｈｔｌｖ抗原を発現する組換え弱毒化ポックスウイルスを含有する免疫原性組成物 | |
| JP2003535577A (ja) | 免疫不全ウイルス用生ウイルスワクチンとしての組換えラブドウイルス | |
| JP2002501369A (ja) | Ｆｉｖワクチン | |
| AU5190900A (en) | Recombinant adenovirus vaccines | |
| Moss | Vaccinia Virus Vectors: Applications to Vaccines | |
| FR2593519A1 (fr) | Vaccin contre le syndrome immunodeficitaire acquis et intermediaires pour la preparation de ce vaccin | |
| WO2002098457A2 (fr) | Rhabdovirus recombinants en tant que vaccins antiviraux actifs pour des virus de l'immunodeficience |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Patent granted |
Ref document number: 114318 Country of ref document: FI |