FI106385B - Förfaranden för att producera protein C - Google Patents
Förfaranden för att producera protein C Download PDFInfo
- Publication number
- FI106385B FI106385B FI945877A FI945877A FI106385B FI 106385 B FI106385 B FI 106385B FI 945877 A FI945877 A FI 945877A FI 945877 A FI945877 A FI 945877A FI 106385 B FI106385 B FI 106385B
- Authority
- FI
- Finland
- Prior art keywords
- protein
- cell line
- human
- adenovirus
- cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6464—Protein C (3.4.21.69)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2523/00—Culture process characterised by temperature
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (10)
1. Förfarande för ökning av produktionen av protein C i en med adenovirus transformerad rekombinanta ) 5 däggdjursvärdcell, kännetecknat av att i nämn-da förfarande odlas nämnda med denovirus transformerade rekombinanta däggdjursvärdcell vid en temperatur pä ca 38 - 39 °C.
2. Förfarande enligt patentkrav 1, k ä n n e -10 tecknat av att den rekombinanta däggdjursvärdcellen är en 293-cell.
3. Förfarande enligt patentkrav 1, k ä n n e -tecknat av att inkuberingstemperaturen är cirka 3 8 °C. 15
4. Förfarande enligt patentkrav 1, k ä n n e - tecknat av att inkuberingstemperaturen är cirka 3 9 °C.
5. Förfarande enligt patentkrav 2, kanne -tecknat av att inkuberingstemperaturen är cirka 20 38 °C.
6. Förfarande enligt patentkrav 2, kanne -tecknat av att inkuberingstemperaturen är cirka 38,5 °C.
7. Förfarande enligt patentkrav 2, k ä n n e - .. 25 tecknat av att inkuberingstemperaturen är cirka 3 9 °C.
8. Förfarande för ökning av gammakarboxylations-graden hos en pä rekombinant sätt producerad protein C-molekyl, kännetecknat av att i nämnda förfa- 30 rande odlas nämnda pä rekombinant sätt producerade protein ; C-molekyl i en rekombinant AV12-värdcell vid en temperatur pä cirka 38 - 39 °C.
9. Förfarande enligt patentkrav 8,känne- v tecknat av att den rekombinanta AV12-värdcellen od- 35 las vid cirka 38 °C. 106385
10. Förfarande enligt patentkrav 8, kanne -tecknat av att den rekombinanta AV12-värdcellen od-las vid cirka 39 °C. ^ 1 < • ·
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/168,035 US5618714A (en) | 1993-12-15 | 1993-12-15 | Methods for producing protein C |
| US16803593 | 1993-12-15 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI945877A0 FI945877A0 (sv) | 1994-12-14 |
| FI945877A FI945877A (sv) | 1995-06-16 |
| FI106385B true FI106385B (sv) | 2001-01-31 |
Family
ID=22609820
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI945877A FI106385B (sv) | 1993-12-15 | 1994-12-14 | Förfaranden för att producera protein C |
Country Status (28)
| Country | Link |
|---|---|
| US (1) | US5618714A (sv) |
| EP (1) | EP0658626B1 (sv) |
| JP (1) | JP3662614B2 (sv) |
| KR (1) | KR950018454A (sv) |
| CN (1) | CN1107887A (sv) |
| AT (1) | ATE196165T1 (sv) |
| AU (1) | AU687914B2 (sv) |
| BR (1) | BR9404991A (sv) |
| CA (1) | CA2138101A1 (sv) |
| CO (1) | CO4340654A1 (sv) |
| CZ (2) | CZ315794A3 (sv) |
| DE (1) | DE69425809T2 (sv) |
| DK (1) | DK0658626T3 (sv) |
| ES (1) | ES2149244T3 (sv) |
| FI (1) | FI106385B (sv) |
| GR (1) | GR3034909T3 (sv) |
| HU (1) | HU219508B (sv) |
| IL (1) | IL111983A0 (sv) |
| NO (1) | NO944846L (sv) |
| NZ (1) | NZ270140A (sv) |
| PE (1) | PE42695A1 (sv) |
| PL (1) | PL176381B1 (sv) |
| PT (1) | PT658626E (sv) |
| RU (1) | RU2110574C1 (sv) |
| SI (1) | SI0658626T1 (sv) |
| UA (1) | UA26870C2 (sv) |
| YU (1) | YU73494A (sv) |
| ZA (1) | ZA949981B (sv) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19724543A1 (de) * | 1997-06-11 | 1998-12-17 | Philips Patentverwaltung | Wechsler-Gerät für Informationsplatten |
| WO2002069232A2 (en) | 2001-02-19 | 2002-09-06 | Merck Patent Gmbh | Method for identification of t-cell epitopes and use for preparing molecules with reeduced immunogenicity |
| AU2003226012B2 (en) | 2002-03-29 | 2007-05-24 | Merck Sharp & Dohme Corp. | Methods of virus production |
| US20070142272A1 (en) * | 2003-01-24 | 2007-06-21 | Zlokovic Berislav V | Neuroprotective activity of activated protein c independent of its anticoagulant activity |
| EP1773371A4 (en) * | 2004-07-23 | 2009-12-30 | Univ Rochester | ACTIVATED PROTEIN C INHIBITS SIDE EFFECTS OF PLASMINOGEN ACTIVATOR IN THE BRAIN |
| WO2006136033A1 (en) * | 2005-06-23 | 2006-12-28 | The University Of British Columbia | Coagulation factor iii polymorphisms associated with prediction of subject outcome and response to therapy |
| WO2007140625A1 (en) * | 2006-06-09 | 2007-12-13 | The University Of British Columbia | Interferon gamma polymorphisms as indicators of subject outcome in critically ill subjects |
| WO2009089620A1 (en) * | 2008-01-15 | 2009-07-23 | The Universityof British Columbia | Protein c rs2069915 as a response predictor to survival and administration of activated protein c or protein c-like compound |
| WO2012068519A2 (en) | 2010-11-19 | 2012-05-24 | Sirius Genomics Inc. | Markers associated with response to activated protein c administration, and uses thereof |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4775624A (en) * | 1985-02-08 | 1988-10-04 | Eli Lilly And Company | Vectors and compounds for expression of human protein C |
| US4959318A (en) * | 1985-06-27 | 1990-09-25 | Zymogenetics, Inc. | Expression of protein C |
| US4968626A (en) * | 1985-08-15 | 1990-11-06 | Board Of Reagents Of The University Of Washington | DNA sequence coding for protein C |
| EP0245949B1 (en) * | 1986-04-09 | 1997-10-29 | Eli Lilly And Company | A method of using eukaryotic expression vectors comprising the bk virus enhancer |
| EP0289586A4 (en) * | 1986-11-17 | 1990-04-10 | New England Medical Ct | INCREASING GAMMA CARBOXYLATION OF RECOMBINANT, VITAMIN K-DEPENDENT PROTEINS. |
| US4992373A (en) * | 1987-12-04 | 1991-02-12 | Eli Lilly And Company | Vectors and compounds for direct expression of activated human protein C |
| ZA889497B (en) * | 1987-12-28 | 1990-08-29 | Lilly Co Eli | Vectors and compounds for expression of zymogen forms of human protein c |
| JPH0246296A (ja) * | 1988-08-09 | 1990-02-15 | Hoechst Japan Ltd | 雑種プロテインｃ及びその製造方法 |
| US4981952A (en) * | 1988-10-04 | 1991-01-01 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
| CA1332049C (en) * | 1988-10-07 | 1994-09-20 | Eli Lilly And Company | Eukaryotic expression |
| ES2047946T3 (es) * | 1989-08-11 | 1994-03-01 | Zymogenetics Inc | Metodos de cultivos celulares para producir proteina c activada. |
| IL97312A (en) * | 1990-02-23 | 1999-01-26 | Lilly Co Eli | A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient |
| AU1180692A (en) * | 1991-01-29 | 1992-08-27 | Teijin Limited | Human protein c expression vector |
-
1993
- 1993-12-15 US US08/168,035 patent/US5618714A/en not_active Expired - Lifetime
-
1994
- 1994-12-14 BR BR9404991A patent/BR9404991A/pt not_active Application Discontinuation
- 1994-12-14 CO CO94056544A patent/CO4340654A1/es unknown
- 1994-12-14 NZ NZ270140A patent/NZ270140A/en unknown
- 1994-12-14 ES ES94309314T patent/ES2149244T3/es not_active Expired - Lifetime
- 1994-12-14 UA UA94129175A patent/UA26870C2/uk unknown
- 1994-12-14 RU RU94043769A patent/RU2110574C1/ru active
- 1994-12-14 DK DK94309314T patent/DK0658626T3/da active
- 1994-12-14 ZA ZA949981A patent/ZA949981B/xx unknown
- 1994-12-14 EP EP94309314A patent/EP0658626B1/en not_active Expired - Lifetime
- 1994-12-14 HU HU9403600A patent/HU219508B/hu not_active IP Right Cessation
- 1994-12-14 DE DE69425809T patent/DE69425809T2/de not_active Expired - Lifetime
- 1994-12-14 FI FI945877A patent/FI106385B/sv active
- 1994-12-14 PE PE1994257065A patent/PE42695A1/es not_active Application Discontinuation
- 1994-12-14 YU YU73494A patent/YU73494A/sh unknown
- 1994-12-14 CZ CZ943157A patent/CZ315794A3/cs unknown
- 1994-12-14 AT AT94309314T patent/ATE196165T1/de not_active IP Right Cessation
- 1994-12-14 AU AU80452/94A patent/AU687914B2/en not_active Ceased
- 1994-12-14 IL IL11198394A patent/IL111983A0/xx unknown
- 1994-12-14 CA CA002138101A patent/CA2138101A1/en not_active Abandoned
- 1994-12-14 KR KR1019940034085A patent/KR950018454A/ko not_active Application Discontinuation
- 1994-12-14 SI SI9430347T patent/SI0658626T1/xx unknown
- 1994-12-14 PT PT94309314T patent/PT658626E/pt unknown
- 1994-12-14 NO NO944846A patent/NO944846L/no not_active Application Discontinuation
- 1994-12-15 CN CN94119106A patent/CN1107887A/zh active Pending
- 1994-12-15 PL PL94306291A patent/PL176381B1/pl unknown
- 1994-12-15 JP JP31160994A patent/JP3662614B2/ja not_active Expired - Fee Related
-
2000
- 2000-11-24 GR GR20000402615T patent/GR3034909T3/el not_active IP Right Cessation
-
2001
- 2001-06-22 CZ CZ20012322A patent/CZ290724B6/cs not_active IP Right Cessation
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