FI105105B - Självreplikerande DNA-vektor för immunisering mot HIV - Google Patents
Självreplikerande DNA-vektor för immunisering mot HIV Download PDFInfo
- Publication number
- FI105105B FI105105B FI980463A FI980463A FI105105B FI 105105 B FI105105 B FI 105105B FI 980463 A FI980463 A FI 980463A FI 980463 A FI980463 A FI 980463A FI 105105 B FI105105 B FI 105105B
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- self
- vector
- papillomavirus
- hiv
- replicating
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
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- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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Claims (11)
1. Självreplikerande rekombinant vektor, kännetecknad av att den innehäller papillomavirus nukleotidsekvenser, vilka väsentligen bestär 5 av (i) en papilloma-virus E1 gen och E2 gen, (ii) en minimal replikon av papillomavirus och (iii) ett minikromosomalt upprätthällningselement av papillomavirus, och 10 en heterolog nukleotidsekvens, som kodar det regulatoriska protei- net NEF, REV eller TAT eller immunologiskt aktiva fragment därav.
2. Självreplikerande vektor enligt patentkrav 1, kännetecknad av att papillomaviruset är papillomavirus av nöt (BPV).
3. Sjävreplikerande vektor enligt patentkrav 1 eller 2, k ä n n e- 15 tecknadavatt den heterologa nukleotidsekvensen kodar NEF-proteinet av HIV-1.
4. Självreplikerande vektor enligt vilket som heist av de föregäende patentkraven, kännetecknad avattEI kontrolleras av sra-promotom eller tymidinkinaspromotorn.
5 C) värdcellen odlas, och D) vektorn tas till vara.
5. Självreplikerande vektor enligt patentkrav 4, känneteck nad av att den är den i figur 2, 3 eller 4 angivna pBNtkREV, pBNsraTAT eller pBNsraNEF.
6. Vaccin för DNA-immunisation mot HIV, kännetecknat av att 25 det innehäller en självreplikerande vektor enligt vilket som heist av patentkraven 1-5.
7. Förfarande för framställning av en självreplikerande vektor enligt vilket som heist av patentkraven 1-5, kännetecknat av att förfarandet omfattar följande:
30 A) en heterolog nukleotidsekvens, som kodar det regulatoriska proteinet NEF, REV eller TAT eller immunologiskt aktiva fragment därav, sätts in i en vektor som innehäller papillomavirus nukleotidsekvenser som väsentligen bestär av - (i) en papilloma-virus E1 gen och E2 gen, 35 - (ii) en minimal replikon av papillomavirus och 21 105105 - (iii) ett minikromosomalt upprätthällningselement av papillomavirus, och B) en värdcell transformeras med den resulterande självreplikeran-de rekombinanta vektorn,
8. Förfarande enligt patentkrav 7, kännetecknat avatt värdcellen är en E.co//-cell.
9. Användning av en självreplikerande vektor enligt vilket som heist 10 av patentkraven 1 - 5 för framställning av ett DNA-immunisationsvaccin mot HIV.
10. Värdcell, kännetecknad avatt den innehäller en självreplikerande vektor enligt vilket som heist av patentkraven 1-5.
11. Värdcell enligt patentkrav 10, kännetecknad avatt den 15 är en bakteriecell eller en däggdjurscell. • ·
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI980463A FI105105B (sv) | 1998-02-27 | 1998-02-27 | Självreplikerande DNA-vektor för immunisering mot HIV |
| CN99804629A CN1295623A (zh) | 1998-02-27 | 1999-02-26 | 用于抗hiv之dna免疫的自我复制的载体 |
| OA1200000232A OA11528A (en) | 1998-02-27 | 1999-02-26 | Self-replicating vector for DNA immunization against HIV. |
| EP99906279A EP1056879A1 (en) | 1998-02-27 | 1999-02-26 | Self-replicating vector for dna immunization against hiv |
| APAP/P/2000/001892A AP2000001892A0 (en) | 1998-02-27 | 1999-02-26 | Self-replicating vector for DNA immunization against HIV. |
| PCT/FI1999/000152 WO1999043841A1 (en) | 1998-02-27 | 1999-02-26 | Self-replicating vector for dna immunization against hiv |
| RU2000122617/13A RU2232815C2 (ru) | 1998-02-27 | 1999-02-26 | Самореплицирующийся рекомбинантный вектор, экспрессирующий регуляторные белки вич, способ его получения, вакцина для иммунизации против вич на основе днк, содержащая указанный вектор, способ лечения или профилактики вич |
| AU26266/99A AU2626699A (en) | 1998-02-27 | 1999-02-26 | Self-replicating vector for dna immunization against hiv |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI980463 | 1998-02-27 | ||
| FI980463A FI105105B (sv) | 1998-02-27 | 1998-02-27 | Självreplikerande DNA-vektor för immunisering mot HIV |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI980463A0 FI980463A0 (fi) | 1998-02-27 |
| FI980463A FI980463A (sv) | 1999-09-27 |
| FI105105B true FI105105B (sv) | 2000-06-15 |
Family
ID=8551073
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI980463A FI105105B (sv) | 1998-02-27 | 1998-02-27 | Självreplikerande DNA-vektor för immunisering mot HIV |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1056879A1 (sv) |
| CN (1) | CN1295623A (sv) |
| AP (1) | AP2000001892A0 (sv) |
| AU (1) | AU2626699A (sv) |
| FI (1) | FI105105B (sv) |
| OA (1) | OA11528A (sv) |
| RU (1) | RU2232815C2 (sv) |
| WO (1) | WO1999043841A1 (sv) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI107737B (sv) * | 1999-12-23 | 2001-09-28 | Atso Raasmaja | Plasmid för att uttrycka en tyrosinhydroxylasgen i hjärnan |
| FI116851B (sv) | 2001-05-03 | 2006-03-15 | Fit Biotech Oyj Plc | Expressionsvektor, dess användningar och förfarande för framställning därav samt produkter som innehåller denna |
| EP1279404A1 (en) * | 2001-07-26 | 2003-01-29 | Istituto Superiore di Sanità | Use of HIV-1 tat, fragments or derivatives thereof, to target or to activate antigen-presenting cells, to deliver cargo molecules for vaccination or to treat other diseases |
| JP2006502704A (ja) | 2002-05-16 | 2006-01-26 | バヴァリアン・ノルディック・アクティーゼルスカブ | Ｈｉｖ調節／アクセサリータンパク質の融合タンパク質 |
| KR101471043B1 (ko) * | 2009-01-08 | 2014-12-09 | 주식회사 바이오리더스 | 안정적인 항시적 고발현 자궁경부암 치료백신용 벡터 및 그에 의해 형질전환된 재조합 유산균 |
| LT3216871T (lt) * | 2011-10-17 | 2022-03-25 | Regeneron Pharmaceuticals, Inc. | Pelės, turinčios apribotą imunoglobulino sunkiąją grandinę |
| AU2017277810A1 (en) * | 2016-06-03 | 2018-12-06 | Temple University - Of The Commonwealth System Of Higher Education | Negative feedback regulation of HIV-1 by gene editing strategy |
| CN110747214B (zh) * | 2019-03-13 | 2021-12-31 | 深圳市臻质医疗科技有限公司 | DNA片段、具有长效表达和细胞特异性结合能力的mRNA-抗体融合分子及其制备方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2241172C (en) * | 1995-12-29 | 2008-08-19 | Estonian Biocentre | Episomal vector and uses thereof |
-
1998
- 1998-02-27 FI FI980463A patent/FI105105B/sv not_active IP Right Cessation
-
1999
- 1999-02-26 OA OA1200000232A patent/OA11528A/en unknown
- 1999-02-26 CN CN99804629A patent/CN1295623A/zh active Pending
- 1999-02-26 RU RU2000122617/13A patent/RU2232815C2/ru not_active IP Right Cessation
- 1999-02-26 EP EP99906279A patent/EP1056879A1/en not_active Withdrawn
- 1999-02-26 WO PCT/FI1999/000152 patent/WO1999043841A1/en not_active Application Discontinuation
- 1999-02-26 AU AU26266/99A patent/AU2626699A/en not_active Abandoned
- 1999-02-26 AP APAP/P/2000/001892A patent/AP2000001892A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FI980463A (sv) | 1999-09-27 |
| EP1056879A1 (en) | 2000-12-06 |
| OA11528A (en) | 2004-05-07 |
| CN1295623A (zh) | 2001-05-16 |
| AU2626699A (en) | 1999-09-15 |
| AP2000001892A0 (en) | 2000-09-30 |
| WO1999043841A1 (en) | 1999-09-02 |
| RU2232815C2 (ru) | 2004-07-20 |
| FI980463A0 (fi) | 1998-02-27 |
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