ES8800989A1 - Un metodo de preparacion de una sonda marcada de acido nucleico. - Google Patents
Un metodo de preparacion de una sonda marcada de acido nucleico.Info
- Publication number
- ES8800989A1 ES8800989A1 ES550736A ES550736A ES8800989A1 ES 8800989 A1 ES8800989 A1 ES 8800989A1 ES 550736 A ES550736 A ES 550736A ES 550736 A ES550736 A ES 550736A ES 8800989 A1 ES8800989 A1 ES 8800989A1
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- detection
- nucleic acids
- hybridization assays
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Jib Cranes (AREA)
- Electronic Switches (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
METODO PARA LA PREPARACION DE UNA SONDA MARCADA DE ACIDO NUCLEICO. CONSISTE EN PONER EN CONTACTO LA SONDA CON UN COMPUESTO QUIMICAMENTE FUNCIONALIZADO QUE SE UNE AL ACIDO NUCLEICO, PREFERIBLEMENTE UN COMPUESTO INTERCALADOR; EN SOMETER LA SONDA Y EL CITADO COMPUESTO DE UNION AL ACIDO NUCLEICO A IRRADIACION FOTOQUIMICA PARA EFECTUAR UNA REACCION COVALENTE; Y EN SOMETER EL PRODUCTO DE REACCION A UNA REACCION ADICIONAL, PARA UNIR UN MARCADOR A TRAVES DE UN COMPUESTO ESPACIADOR AL RADICAL QUIMICAMENTE FUNCIONALIZADO. LA SONDA MARCADA DE ACIDO NUCLEICO COMPRENDE UN COMPONENTE ACIDO NUCLEICO, UN LIGANDO QUE SE UNE AL ACIDO NUCLEICO FOTOQUIMICAMENTE ENLAZADO AL COMPONENTE ACIDO NUCLEICO, UN MARCADOR, Y UN ESPACIADOR QUE ENLAZA EL LIGANDO CON EL MARCADOR.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US69033685A | 1985-01-10 | 1985-01-10 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES550736A0 ES550736A0 (es) | 1987-12-01 |
ES8800989A1 true ES8800989A1 (es) | 1987-12-01 |
Family
ID=24772062
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES550736A Expired ES8800989A1 (es) | 1985-01-10 | 1986-01-08 | Un metodo de preparacion de una sonda marcada de acido nucleico. |
Country Status (11)
Country | Link |
---|---|
US (1) | US4950744A (es) |
EP (1) | EP0187332B1 (es) |
JP (1) | JPS61164160A (es) |
AT (1) | ATE40572T1 (es) |
AU (1) | AU588563B2 (es) |
DE (1) | DE3568063D1 (es) |
DK (1) | DK9586A (es) |
ES (1) | ES8800989A1 (es) |
FI (1) | FI860077A (es) |
NZ (1) | NZ214745A (es) |
ZA (1) | ZA86164B (es) |
Families Citing this family (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5605800A (en) * | 1978-04-13 | 1997-02-25 | Institut Pasteur | Method of detecting and characterizing a nucleic acid or a sequence of the latter, and enzymatic reactant for the application of this method |
FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
US4822731A (en) * | 1986-01-09 | 1989-04-18 | Cetus Corporation | Process for labeling single-stranded nucleic acids and hybridizaiton probes |
SE454781B (sv) * | 1986-10-17 | 1988-05-30 | Wallac Oy | Hybridiseringsforfarande for detektion av polynukleotidsekvens |
AU601021B2 (en) * | 1987-03-11 | 1990-08-30 | Molecular Diagnostics, Inc. | Assay for necleic acid sequences in a sample |
JPH0827281B2 (ja) * | 1987-06-09 | 1996-03-21 | エヌオーケー株式会社 | Dnaの簡易検出法 |
US6004745A (en) * | 1987-09-21 | 1999-12-21 | Gen-Probe Incorporated | Hybridization protection assay |
US5283174A (en) * | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
US5639604A (en) * | 1987-09-21 | 1997-06-17 | Gen-Probe Incorporated | Homogeneous protection assay |
EP0383803B1 (en) * | 1987-10-28 | 2000-05-03 | Howard Florey Institute Of Experimental Physiology And Medicine | Oligonucleotide-polyamide conjugates |
US5525465A (en) * | 1987-10-28 | 1996-06-11 | Howard Florey Institute Of Experimental Physiology And Medicine | Oligonucleotide-polyamide conjugates and methods of production and applications of the same |
US5449602A (en) * | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
DE3940597A1 (de) * | 1989-12-08 | 1991-06-13 | Bayer Ag | Verfahren zur herstellung von di- und trialkyl-4'-phthalimidomethyl-furocumarinen |
US5677440A (en) * | 1990-07-16 | 1997-10-14 | Howard Florey Institute Of Experimental Physiology And Medicine | Oligonucleotide-polyamide conjugates |
DE4132466A1 (de) * | 1991-09-30 | 1993-04-01 | Bayer Ag | Photochemisch verknuepfbare polymere |
DE4132467A1 (de) * | 1991-09-30 | 1993-04-01 | Bayer Ag | Fotochemisch reaktive initiatoren fuer die radikalische polymerisation |
DE4222254A1 (de) * | 1992-07-07 | 1994-01-13 | Bayer Ag | Fotochemische Markierung von Nukleinsäuren mit Digoxigenin-Reagenzien und ihre Verwendung in Gensondentestsystemen |
DE4222255A1 (de) * | 1992-07-07 | 1994-01-13 | Bayer Ag | Fotochemische Markierung von Nukleinsäuren mit Europiumchelat-Reagenzien und ihre Verwendung in Gensondentestsystemen |
US5731148A (en) * | 1995-06-07 | 1998-03-24 | Gen-Probe Incorporated | Adduct protection assay |
EP0822261B1 (en) * | 1996-07-29 | 2002-07-03 | Academia Sinica | Methods for rapid antimicrobial susceptibility testing |
JP4981197B2 (ja) * | 1996-10-08 | 2012-07-18 | クレアテク バイオテクノロジー ベー.ファー. | ヌクレオチドを標識付けする方法、標識ヌクレオチド及び有用な中間体 |
WO1998052961A1 (en) * | 1997-05-19 | 1998-11-26 | Mirus Corporation | Method for single-step attachment of a label to target molecules |
EP1947105A1 (en) * | 1997-05-19 | 2008-07-23 | Mirus Bio Corporation | Method for single-step attachment of a label to target molecules |
US6646149B1 (en) * | 1997-07-15 | 2003-11-11 | Nicolaas M. J. Vermeulin | Polyamine analogues as therapeutic and diagnostic agents |
US6284220B1 (en) * | 1999-06-03 | 2001-09-04 | The General Hospital Corporation | Noninvasive imaging of nucleic acid vectors |
US7049142B1 (en) | 1999-08-02 | 2006-05-23 | Mirus Corporation | Gene expression with covalently modified polynucleotides |
WO2001008710A1 (en) * | 1999-08-02 | 2001-02-08 | Mirus Corporation | Gene expression with covalently modified polynucleotides |
GB0016836D0 (en) * | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (1) |
USRE43327E1 (en) | 2001-01-08 | 2012-04-24 | Aminex Therapeutics, Inc. | Hydrophobic polyamine analogs and methods for their use |
US6664043B2 (en) * | 2001-07-03 | 2003-12-16 | Bayer Corporation | Acridinium ester labels having hydrophilic modifiers |
US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
US7166478B2 (en) * | 2002-03-12 | 2007-01-23 | Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. | Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses |
US6924373B2 (en) * | 2003-05-02 | 2005-08-02 | Asiagen Corporation | DNA labeling reagents, acridinium-9-carboxamide derivatives and process of preparing DNA labeling compounds |
CN105001665B (zh) * | 2014-04-15 | 2017-05-31 | 苏州嘉恒生物科技有限公司 | 一种新型核酸染料的制备及其应用 |
EP3432914B1 (en) | 2016-03-25 | 2020-09-23 | Aminex Therapeutics, Inc. | Bioavailable polyamines |
WO2022072586A1 (en) | 2020-09-30 | 2022-04-07 | Aminex Therapeutics, Inc. | Combination drug substance of polyamine transport inhibitor and dfmo |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1219824A (en) * | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
GB8306426D0 (en) * | 1983-03-09 | 1983-04-13 | Malcolm A D B | Detecting polynucleotide sequence |
CA1222680A (en) * | 1983-07-05 | 1987-06-09 | Nanibhushan Dattagupta | Testing dna samples for particular nucleotide sequences |
US4737454A (en) * | 1983-07-14 | 1988-04-12 | Molecular Diagnostics, Inc. | Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays |
US4626501A (en) * | 1983-09-02 | 1986-12-02 | Integrated Genetics, Inc. | Labeled DNA |
US4599303A (en) * | 1983-12-12 | 1986-07-08 | Hri Associates, Inc. | Nucleic acid hybridization assay employing probes crosslinkable to target sequences |
US4617261A (en) * | 1984-03-21 | 1986-10-14 | Cetus Corporation | Process for labeling nucleic acids and hybridization probes |
US4582789A (en) * | 1984-03-21 | 1986-04-15 | Cetus Corporation | Process for labeling nucleic acids using psoralen derivatives |
AU580042B2 (en) * | 1984-03-22 | 1988-12-22 | Bresatec Limited | Non-radioactive biological probes |
-
1985
- 1985-12-18 EP EP85116199A patent/EP0187332B1/en not_active Expired
- 1985-12-18 AT AT85116199T patent/ATE40572T1/de not_active IP Right Cessation
- 1985-12-18 DE DE8585116199T patent/DE3568063D1/de not_active Expired
-
1986
- 1986-01-07 NZ NZ214745A patent/NZ214745A/xx unknown
- 1986-01-08 AU AU52146/86A patent/AU588563B2/en not_active Expired - Fee Related
- 1986-01-08 JP JP61000819A patent/JPS61164160A/ja active Pending
- 1986-01-08 ES ES550736A patent/ES8800989A1/es not_active Expired
- 1986-01-08 FI FI860077A patent/FI860077A/fi not_active Application Discontinuation
- 1986-01-09 DK DK9586A patent/DK9586A/da not_active Application Discontinuation
- 1986-01-09 ZA ZA86164A patent/ZA86164B/xx unknown
-
1987
- 1987-03-18 US US07/027,384 patent/US4950744A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU588563B2 (en) | 1989-09-21 |
DE3568063D1 (en) | 1989-03-09 |
ZA86164B (en) | 1986-09-24 |
FI860077A0 (fi) | 1986-01-08 |
EP0187332A3 (en) | 1987-02-25 |
US4950744A (en) | 1990-08-21 |
AU5214686A (en) | 1986-07-17 |
EP0187332A2 (en) | 1986-07-16 |
JPS61164160A (ja) | 1986-07-24 |
EP0187332B1 (en) | 1989-02-01 |
ES550736A0 (es) | 1987-12-01 |
DK9586D0 (da) | 1986-01-09 |
NZ214745A (en) | 1989-06-28 |
DK9586A (da) | 1986-07-11 |
ATE40572T1 (de) | 1989-02-15 |
FI860077A (fi) | 1986-07-11 |
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