ES8800344A1 - Un metodo de produccion de actividad de isopenicilina n sintetasa en una celula huesped - Google Patents

Un metodo de produccion de actividad de isopenicilina n sintetasa en una celula huesped

Info

Publication number
ES8800344A1
ES8800344A1 ES554218A ES554218A ES8800344A1 ES 8800344 A1 ES8800344 A1 ES 8800344A1 ES 554218 A ES554218 A ES 554218A ES 554218 A ES554218 A ES 554218A ES 8800344 A1 ES8800344 A1 ES 8800344A1
Authority
ES
Spain
Prior art keywords
synthetase
isopenicillin
acremonium
novel
expression vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES554218A
Other languages
English (en)
Spanish (es)
Other versions
ES554218A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of ES554218A0 publication Critical patent/ES554218A0/es
Publication of ES8800344A1 publication Critical patent/ES8800344A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
ES554218A 1985-04-22 1986-04-21 Un metodo de produccion de actividad de isopenicilina n sintetasa en una celula huesped Expired ES8800344A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72587085A 1985-04-22 1985-04-22
US79938485A 1985-11-18 1985-11-18

Publications (2)

Publication Number Publication Date
ES554218A0 ES554218A0 (es) 1987-11-01
ES8800344A1 true ES8800344A1 (es) 1987-11-01

Family

ID=27111238

Family Applications (3)

Application Number Title Priority Date Filing Date
ES554218A Expired ES8800344A1 (es) 1985-04-22 1986-04-21 Un metodo de produccion de actividad de isopenicilina n sintetasa en una celula huesped
ES557658A Expired ES8802585A1 (es) 1985-04-22 1987-08-11 Un procedimiento para la preparacion de un plasmido que codifica isopenicilina n sintetasa
ES557829A Expired ES8900142A1 (es) 1985-04-22 1988-04-15 Un procedimiento para aislar el plasmido it335

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES557658A Expired ES8802585A1 (es) 1985-04-22 1987-08-11 Un procedimiento para la preparacion de un plasmido que codifica isopenicilina n sintetasa
ES557829A Expired ES8900142A1 (es) 1985-04-22 1988-04-15 Un procedimiento para aislar el plasmido it335

Country Status (14)

Country Link
EP (1) EP0200425B1 (US07488766-20090210-C00029.png)
JP (1) JPH0787788B2 (US07488766-20090210-C00029.png)
KR (1) KR860008287A (US07488766-20090210-C00029.png)
CN (1) CN86102604A (US07488766-20090210-C00029.png)
AU (2) AU592791B2 (US07488766-20090210-C00029.png)
BG (2) BG45047A3 (US07488766-20090210-C00029.png)
CA (1) CA1309673C (US07488766-20090210-C00029.png)
DE (1) DE3683080D1 (US07488766-20090210-C00029.png)
DK (1) DK178386A (US07488766-20090210-C00029.png)
ES (3) ES8800344A1 (US07488766-20090210-C00029.png)
FI (1) FI95810C (US07488766-20090210-C00029.png)
HU (1) HU202279B (US07488766-20090210-C00029.png)
IE (1) IE58780B1 (US07488766-20090210-C00029.png)
IL (1) IL78531A0 (US07488766-20090210-C00029.png)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3177213D1 (de) 1980-04-03 1990-10-18 Biogen Inc Dns-sequenzen, rekombinante dns-molekuele und verfahren zur herstellung von dem menschlichen fibroblast-interferon.
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum
DE3789880T2 (de) * 1986-01-31 1994-09-08 Beecham Group Plc Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen.
CA1327170C (en) * 1987-03-04 1994-02-22 Stephen Wyatt Queener Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase and deacetylcephalosporin c synthetase
GB2208077A (en) * 1987-04-24 1989-02-22 Antibioticos Sa Isopenicillin n synthetase gene derived from aspergillus nidulans
EP0303364A1 (en) * 1987-08-10 1989-02-15 The Governors of the University of Alberta Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene from streptomyces clavuligerus
US4885252A (en) * 1987-09-08 1989-12-05 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from aspergillus nidulans
US4950603A (en) * 1987-11-02 1990-08-21 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from Streptomyces lipmanii
US6258555B1 (en) 1987-12-09 2001-07-10 Beecham Group P.L.C. DNA encoding ACV synthetase
GB8728811D0 (en) * 1987-12-09 1988-01-27 Beecham Group Plc Novel substance
KR0171897B1 (ko) * 1989-12-27 1999-02-01 후지사와 도모끼찌로 7-아미노세펨 화합물 또는 그 염류의 제조 방법
ATE180279T1 (de) * 1990-01-12 1999-06-15 Glaxo Group Ltd Für ein enzym der antibiotika-biosynthese kodierende dna-sequenz
JP3764166B2 (ja) * 1994-09-28 2006-04-05 ノボザイムス アクティーゼルスカブ 二次代謝産物を産生するための方法
DE19755302A1 (de) * 1997-12-12 1999-06-17 Hoechst Marion Roussel De Gmbh Neuer Transkriptionsfaktor CPCR1 zur Regulation des Isopenicillin N-Synthetase-Gens (pcbC) und des AC-/ACV-Synthetase-Gens (pcbAB)
AU4181000A (en) * 1999-04-02 2000-10-23 Du Pont Pharmaceuticals Company Novel lactam inhibitors of matrix metalloproteinases, tnf-alpha, and aggrecanase

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3330003A1 (de) * 1982-10-21 1984-10-31 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Dna-sequenz und dna-strukturen und sie verwendendes verfahren zur herstellung von penicillin-acylase
CA1278540C (en) * 1983-07-22 1991-01-02 Eli Lilly And Company Modified antibiotic resistance gene
US4762786A (en) * 1984-09-27 1988-08-09 Eli Lilly And Company Vectors and conditions which allow genetic transformation of cephalosporium
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum

Also Published As

Publication number Publication date
AU4234789A (en) 1990-01-11
AU5643186A (en) 1986-10-30
HUT40705A (en) 1987-01-28
ES8802585A1 (es) 1988-08-16
FI95810B (fi) 1995-12-15
EP0200425A3 (en) 1988-07-06
DK178386D0 (da) 1986-04-18
IL78531A0 (en) 1986-08-31
ES554218A0 (es) 1987-11-01
ES557829A0 (es) 1989-01-16
FI861653A0 (fi) 1986-04-18
IE58780B1 (en) 1993-11-17
EP0200425A2 (en) 1986-11-05
BG45047A3 (bg) 1989-03-15
CN86102604A (zh) 1986-11-26
ES8900142A1 (es) 1989-01-16
JPS61254192A (ja) 1986-11-11
AU619596B2 (en) 1992-01-30
JPH0787788B2 (ja) 1995-09-27
FI95810C (fi) 1996-03-25
KR860008287A (ko) 1986-11-14
EP0200425B1 (en) 1991-12-27
DK178386A (da) 1986-10-23
CA1309673C (en) 1992-11-03
FI861653A (fi) 1986-10-23
HU202279B (en) 1991-02-28
AU592791B2 (en) 1990-01-25
IE861029L (en) 1986-10-22
ES557658A0 (es) 1988-08-16
BG45219A3 (bg) 1989-04-14
DE3683080D1 (de) 1992-02-06

Similar Documents

Publication Publication Date Title
ES8800344A1 (es) Un metodo de produccion de actividad de isopenicilina n sintetasa en una celula huesped
IL80710A0 (en) Recombinant dna expression vectors and dna compounds that encode isopenicillin n synthetase from penicillium chrysogenum
EP0136829A3 (en) Improved expression vector and use thereof
AU560965B2 (en) Cloning vectors for expression of exogenous protein
JPS57181099A (en) Bacillus cloning vector, recombinant dna molecule, bacillus host transformed thereby and manufacture of polypeptide expressing dna order and being coded thereby
ES8302774A1 (es) Un metodo para producir un vector de clonacion mejorado.
JPS5648884A (US07488766-20090210-C00029.png)
AU555574B2 (en) Recombinant dna cloning vector and eukaryotic/prokaryotic transformants
ES8105391A1 (es) Un procedimiento para la preparacion de un vector de trans- ferencia.
IL79837A (en) Upstream activation sequence(uas)elements from the yeast ph05 promoter,yeast hybrid promoters and vectors,hosts transformed therewith and processes for the preparation thereof and of recombinant polypeptides
IL85020A (en) Pancreatic secretory trypsin inhibitor variants, their production, expression vectors and recombinant hosts therefor and pharmaceutical compositions containing them
GB2162851B (en) Novel b-urogastrone gene, corresponding recombinant plasmids, corresponding transformants and preparations thereof and of b-urogastrone.
DE3666838D1 (en) Plasmid vectors for expression in escherichia coli and/or bacillus subtilis
HU896734D0 (en) Process for the preparation of dna encoding for isopenicillin n epimerase (racemase) and recombinant dna expression vector
AU6103086A (en) Cloning and characterization of the bovine interleukin-2 gene
EP0255320A3 (en) Production of proteins in myeloma cells
DE3479816D1 (en) A promotor and use thereof
IL72449A0 (en) Plasmid vector and an escherichia coli transformant obtained therewith
EP0595334A3 (en) Mutated AOX2 promoter, container vector, cells transformed by this vector and production of heterologous proteins.
HUT36498A (en) Gene of modeified antibiotic resistency
ES8802075A1 (es) Procedimiento para la multiplicacion de un adn heterologo

Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20050915