ES8705033A1 - Un metodo para preparar una vacuna para uso en el tratamiento prevencion de diarrea porcina neonatol. - Google Patents
Un metodo para preparar una vacuna para uso en el tratamiento prevencion de diarrea porcina neonatol.Info
- Publication number
- ES8705033A1 ES8705033A1 ES549531A ES549531A ES8705033A1 ES 8705033 A1 ES8705033 A1 ES 8705033A1 ES 549531 A ES549531 A ES 549531A ES 549531 A ES549531 A ES 549531A ES 8705033 A1 ES8705033 A1 ES 8705033A1
- Authority
- ES
- Spain
- Prior art keywords
- cloning
- dna
- vaccine
- large fragment
- vehicle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000010367 cloning Methods 0.000 title abstract 4
- 239000012634 fragment Substances 0.000 title abstract 3
- 101710177917 Fimbrial protein Proteins 0.000 title abstract 2
- 229960005486 vaccine Drugs 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 3
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000206602 Eukaryota Species 0.000 abstract 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 238000005192 partition Methods 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Abstract
METODO DE PREPARAR UNA VACUNA PARA USO EN EL TRATAMIENTO O PREVENCION DE DIARREA PORCINA NEONATAL. CONSISTE EN: A) PURIFICAR ANTIGENOS FIMBRICOS DISTINTOS DE 987P; B) CULTIVAR CELULAS HOSPEDANTES RECOMBINANTES DE E. COLI QUE EXPRESAN EL GEN 987P EN UN MEDIO QUE COMPRENDE SALES DE EXTRACTO DE LEVADURA Y TRIPTONA, Y UNA FUENTE DE CARBONO; C) AISLAR EL PRODUCTO FIMBRIAL SEPARANDO DEL CALDO DE CULTIVO LA BIOMASA RESIDUAL POR CENTRIFUGACION Y LAVANDO LA SUSPENSION DE FIMBRIAS CON SOLUCIONES TAMPON; D) AÑADIR LA PROTEINA FIMBRICA 987P, RESULTANTE DE LA OPERACION C), A LOS ANTIGENOS FIMBRICOS PURIFICADOS DISTINTOS DE 987P; Y E) AÑADIR AL MATERIAL RESULTANTE UN VEHICULO, DILUYENTE O COADYUVANTE. TIENE APLICACIONES EN EL CAMPO DE LA INVESTIGACION VETERINARIA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AUPG840384 | 1984-12-04 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8705033A1 true ES8705033A1 (es) | 1987-04-16 |
ES549531A0 ES549531A0 (es) | 1987-04-16 |
Family
ID=3770864
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES549531A Expired ES8705033A1 (es) | 1984-12-04 | 1985-12-03 | Un metodo para preparar una vacuna para uso en el tratamiento prevencion de diarrea porcina neonatol. |
Country Status (14)
Country | Link |
---|---|
EP (1) | EP0242359B1 (es) |
JP (1) | JPS62501675A (es) |
KR (1) | KR870700021A (es) |
CN (1) | CN1017451B (es) |
AU (1) | AU596958B2 (es) |
DE (1) | DE3587473T2 (es) |
DK (1) | DK370186A (es) |
ES (1) | ES8705033A1 (es) |
FI (1) | FI863145A (es) |
HK (1) | HK70594A (es) |
NZ (1) | NZ214428A (es) |
SG (1) | SG87794G (es) |
WO (1) | WO1986003410A1 (es) |
ZA (1) | ZA859297B (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU616662B2 (en) * | 1987-04-27 | 1991-11-07 | Commonwealth Scientific And Industrial Research Organisation | Peptide production by protein engineering |
EP0314224B1 (en) * | 1987-10-26 | 1992-05-27 | Akzo N.V. | Vaccine against e.coli septicaemia in poultry |
HU221664B1 (hu) * | 1999-03-31 | 2002-12-28 | MTA Állatorvostudományi Kutatóintézete | Élő, szájon át adható Escherichia coli vakcina készítésére alkalmas törzs a sertések választási hasmenésének megelőzésére és a törzs előállítására alkalmas eljárás |
CN107693786B (zh) * | 2017-11-07 | 2021-06-29 | 聊城大学 | 特异性防治驴驹大肠杆菌性腹泻的复合疫苗 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2094314A (en) * | 1981-02-05 | 1982-09-15 | Wellcome Found | Recombinant DNA |
NZ199866A (en) * | 1981-03-09 | 1984-11-09 | Cetus Corp | Vaccine for prevention of gastroenteric disease:non-pathogenic strain genetically engineered to comprise genes for adhesin and/or toxoids |
DK242883A (da) * | 1982-06-02 | 1983-12-03 | Cetus Corp | Fremgangsmaade til frembringelse af konstruerede rekombinant plasmider |
DK410783D0 (da) * | 1982-09-16 | 1983-09-09 | Benzon A Salfred | Fremgangsmade til stabilisering af plasmider |
JPH074257B2 (ja) * | 1983-06-17 | 1995-01-25 | 三菱油化株式会社 | 新規なプラスミド |
EP0180192B1 (en) * | 1984-10-29 | 1992-02-05 | Research Association For Utilization Of Light Oil | Novel plasmids |
JPH0694095A (ja) * | 1992-09-08 | 1994-04-05 | Mitsubishi Steel Mfg Co Ltd | 回転抵抗調整機構 |
-
1985
- 1985-12-03 ES ES549531A patent/ES8705033A1/es not_active Expired
- 1985-12-04 JP JP61500188A patent/JPS62501675A/ja active Pending
- 1985-12-04 CN CN85109506A patent/CN1017451B/zh not_active Expired
- 1985-12-04 EP EP86900017A patent/EP0242359B1/en not_active Expired - Lifetime
- 1985-12-04 AU AU52097/86A patent/AU596958B2/en not_active Expired
- 1985-12-04 KR KR860700521A patent/KR870700021A/ko not_active Application Discontinuation
- 1985-12-04 ZA ZA859297A patent/ZA859297B/xx unknown
- 1985-12-04 DE DE86900017T patent/DE3587473T2/de not_active Expired - Fee Related
- 1985-12-04 NZ NZ214428A patent/NZ214428A/xx unknown
- 1985-12-04 WO PCT/AU1985/000306 patent/WO1986003410A1/en active IP Right Grant
-
1986
- 1986-08-01 FI FI863145A patent/FI863145A/fi not_active Application Discontinuation
- 1986-08-04 DK DK370186A patent/DK370186A/da not_active Application Discontinuation
-
1994
- 1994-06-30 SG SG87794A patent/SG87794G/en unknown
- 1994-07-21 HK HK70594A patent/HK70594A/xx unknown
Also Published As
Publication number | Publication date |
---|---|
ZA859297B (en) | 1986-08-27 |
CN85109506A (zh) | 1986-09-10 |
EP0242359A4 (en) | 1989-02-23 |
DE3587473D1 (es) | 1993-08-26 |
JPS62501675A (ja) | 1987-07-09 |
FI863145A0 (fi) | 1986-08-01 |
DE3587473T2 (de) | 1993-11-04 |
SG87794G (en) | 1994-10-14 |
EP0242359B1 (en) | 1993-07-21 |
KR870700021A (ko) | 1987-02-28 |
FI863145A (fi) | 1986-08-01 |
NZ214428A (en) | 1989-04-26 |
AU5209786A (en) | 1986-07-01 |
EP0242359A1 (en) | 1987-10-28 |
CN1017451B (zh) | 1992-07-15 |
DK370186A (da) | 1987-03-20 |
HK70594A (en) | 1994-07-29 |
DK370186D0 (da) | 1986-08-04 |
WO1986003410A1 (en) | 1986-06-19 |
ES549531A0 (es) | 1987-04-16 |
AU596958B2 (en) | 1990-05-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19971103 |