IE894177L - Plasmids with a translation start-stop-start codon¹configuration for the expression of proteins in E. coli - Google Patents

Plasmids with a translation start-stop-start codon¹configuration for the expression of proteins in E. coli

Info

Publication number
IE894177L
IE894177L IE894177A IE417789A IE894177L IE 894177 L IE894177 L IE 894177L IE 894177 A IE894177 A IE 894177A IE 417789 A IE417789 A IE 417789A IE 894177 L IE894177 L IE 894177L
Authority
IE
Ireland
Prior art keywords
expression
proteins
plasmids
coli
stop
Prior art date
Application number
IE894177A
Original Assignee
Behringwerke Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke Ag filed Critical Behringwerke Ag
Publication of IE894177L publication Critical patent/IE894177L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Abstract

The invention relates to expression plasmids which bring about a considerable increase in expression of proteins, in particular of the thermolabile E.coli enterotoxin subunit B (LT-B). This entails a strong promoter being placed in front of the DNA coding for LT-B so that the so-called Shine-Dalgarno sequence GGGA of the LT-B gene is located six base-pairs (bp) in front of the translation start of the LT-B, and where the stop codon TGA of the LT-A sequence, which precedes in the natural case, is retained. [EP0379710A1]
IE894177A 1988-12-24 1989-12-21 Plasmids with a translation start-stop-start codon¹configuration for the expression of proteins in E. coli IE894177L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3843894A DE3843894A1 (en) 1988-12-24 1988-12-24 PLASMIDE WITH TRANSLATION-START-STOP-START CODON CONFIGURATION FOR EXPRESSION OF PROTEINS IN E.COLI

Publications (1)

Publication Number Publication Date
IE894177L true IE894177L (en) 1990-06-24

Family

ID=6370260

Family Applications (1)

Application Number Title Priority Date Filing Date
IE894177A IE894177L (en) 1988-12-24 1989-12-21 Plasmids with a translation start-stop-start codon¹configuration for the expression of proteins in E. coli

Country Status (9)

Country Link
EP (1) EP0379710A1 (en)
JP (1) JPH02231087A (en)
KR (1) KR900009988A (en)
AU (1) AU624667B2 (en)
CA (1) CA2006534A1 (en)
DE (1) DE3843894A1 (en)
DK (1) DK661489A (en)
IE (1) IE894177L (en)
PT (1) PT92656A (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3901681A1 (en) * 1989-01-21 1990-07-26 Behringwerke Ag SIGNAL PEPTIDE FOR THE SECRETION OF PEPTIDES IN ESCHERICHIA COLI, METHOD FOR ITS EFFECT AND ITS USE
JPH03187383A (en) * 1989-09-04 1991-08-15 Takeda Chem Ind Ltd Manifestation plasmid and use thereof
CA2444298A1 (en) 2001-04-13 2002-10-24 Zymogenetics, Inc. Method for enhancing the translation and expression of recombinant proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
ZA851626B (en) * 1984-03-06 1986-10-29 Lilly Co Eli Recombinant dna expression vectors and method for gene expression
CA1340372C (en) * 1984-07-09 1999-02-02 John D. Clements Production of e. coli lt-b enterotoxin subunit

Also Published As

Publication number Publication date
DK661489D0 (en) 1989-12-22
AU4720289A (en) 1990-06-28
KR900009988A (en) 1990-07-06
PT92656A (en) 1990-05-31
AU624667B2 (en) 1992-06-18
EP0379710A1 (en) 1990-08-01
DK661489A (en) 1990-06-25
DE3843894A1 (en) 1990-06-28
JPH02231087A (en) 1990-09-13
CA2006534A1 (en) 1990-06-24

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