ES8303443A1 - "un procedimiento para la purificacion de quimopapaina bruta". - Google Patents
"un procedimiento para la purificacion de quimopapaina bruta".Info
- Publication number
- ES8303443A1 ES8303443A1 ES512113A ES512113A ES8303443A1 ES 8303443 A1 ES8303443 A1 ES 8303443A1 ES 512113 A ES512113 A ES 512113A ES 512113 A ES512113 A ES 512113A ES 8303443 A1 ES8303443 A1 ES 8303443A1
- Authority
- ES
- Spain
- Prior art keywords
- chymopapain
- purified
- disc
- proteolytically
- toxic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PROCEDIMIENTO PARA LA PURIFICACION DE QUIMOPAPAINA BRUTA PARA OBTENER QUIMOPAPAINA DE TOXICIDAD REDUCIDA. CONSISTE EN PESAR UNA DISOLUCION ACUOSA TAMPONADA DE QUIMOPAPAINA BRUTA A TRAVES DE UN CAMBIADOR DE CATIONES DEBILMENTE ACIDO, CONSTITUIDO POR UN GEL DE AGAROSA RETICULADA SUSTITUIDA CON CARBOXIMETILO, A PH 6,5 A 7,5. ELUIR CON DISOLUCION ACUOSA TAMPON SIMILAR. DESECHAR UNA PRIMERA SERIE DE FRACCIONES HASTA QUE EL ELUYENTE TENGA UNA CONCENTRACION SALINA ENTRE 0,25 Y 0,4 M. RETENER LAS SERIES ULTERIORES HASTA AGOTAMIENTO DE LA QUIMOPAPAINA Y SEPARAR DE LAS MISMAS LAS SALES INORGANICAS Y LOS COMPONENTES DEL TAMPON. FINALMENTE, LIOFILIZAR LA DISOLUCION RESULTANTE. ESTE COMPUESTO TIENE APLICACIONES FARMACOLOGICAS PARA EL TRATAMIENTO DE DISCOS INTERVERTEBRALES ANORMALES.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/263,197 US4439423A (en) | 1981-05-13 | 1981-05-13 | Chymopapain and method for its use |
US06/263,196 US4374926A (en) | 1981-05-13 | 1981-05-13 | Method for the production of improved chymopapain |
Publications (2)
Publication Number | Publication Date |
---|---|
ES512113A0 ES512113A0 (es) | 1983-02-01 |
ES8303443A1 true ES8303443A1 (es) | 1983-02-01 |
Family
ID=26949703
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES512113A Expired ES8303443A1 (es) | 1981-05-13 | 1982-05-12 | "un procedimiento para la purificacion de quimopapaina bruta". |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0065395B1 (es) |
AR (1) | AR229179A1 (es) |
AU (1) | AU555487B2 (es) |
DE (1) | DE3278934D1 (es) |
DK (1) | DK163525C (es) |
ES (1) | ES8303443A1 (es) |
GB (2) | GB2098997B (es) |
MX (1) | MX157870A (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2156821A (en) * | 1984-04-03 | 1985-10-16 | Simmons James W | Process for purifying chymopapain |
GB2193720B (en) * | 1986-08-15 | 1990-09-19 | Agricultural & Food Res | Payaya proteinase b separation and uses |
GB8909836D0 (en) * | 1989-04-28 | 1989-06-14 | Boots Co Plc | Therapeutic agent |
CN112813057A (zh) * | 2021-01-08 | 2021-05-18 | 海南大学 | 一种木瓜酶制剂的高效制备方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3558433A (en) * | 1967-11-07 | 1971-01-26 | Baxter Laboratories Inc | Process for purification of chymopapain |
-
1982
- 1982-05-07 EP EP82302339A patent/EP0065395B1/en not_active Expired
- 1982-05-07 DE DE8282302339T patent/DE3278934D1/de not_active Expired
- 1982-05-11 AR AR289363A patent/AR229179A1/es active
- 1982-05-12 ES ES512113A patent/ES8303443A1/es not_active Expired
- 1982-05-12 AU AU83641/82A patent/AU555487B2/en not_active Expired
- 1982-05-12 GB GB8213797A patent/GB2098997B/en not_active Expired
- 1982-05-12 MX MX192651A patent/MX157870A/es unknown
- 1982-05-13 DK DK215482A patent/DK163525C/da not_active IP Right Cessation
-
1984
- 1984-07-04 GB GB08417000A patent/GB2145518B/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
AU555487B2 (en) | 1986-09-25 |
DK163525B (da) | 1992-03-09 |
GB2098997A (en) | 1982-12-01 |
AU8364182A (en) | 1982-11-18 |
ES512113A0 (es) | 1983-02-01 |
MX157870A (es) | 1988-12-19 |
DK215482A (da) | 1982-11-14 |
EP0065395B1 (en) | 1988-08-24 |
EP0065395A2 (en) | 1982-11-24 |
DK163525C (da) | 1992-07-27 |
GB2145518A (en) | 1985-03-27 |
GB2145518B (en) | 1985-11-06 |
AR229179A1 (es) | 1983-06-30 |
DE3278934D1 (en) | 1988-09-29 |
EP0065395A3 (en) | 1983-07-27 |
GB2098997B (en) | 1985-11-06 |
GB8417000D0 (en) | 1984-08-08 |
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