ES8103174A1 - Un metodo y su correspondiente equipo para la deteccion o determinacion de una incognita analizable. - Google Patents

Un metodo y su correspondiente equipo para la deteccion o determinacion de una incognita analizable.

Info

Publication number
ES8103174A1
ES8103174A1 ES487849A ES487849A ES8103174A1 ES 8103174 A1 ES8103174 A1 ES 8103174A1 ES 487849 A ES487849 A ES 487849A ES 487849 A ES487849 A ES 487849A ES 8103174 A1 ES8103174 A1 ES 8103174A1
Authority
ES
Spain
Prior art keywords
specific binding
enzyme
components
testkit
bindable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES487849A
Other languages
English (en)
Other versions
ES487849A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever NV filed Critical Unilever NV
Publication of ES487849A0 publication Critical patent/ES487849A0/es
Publication of ES8103174A1 publication Critical patent/ES8103174A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

METODO Y EQUIPO PARA DETECTAR Y DETERMINAR LOS AGENTES LIGANTES PROTEINICOS ESPECIFICOS Y MATERIALES LIGABLES A ELLOS. EL METODO DE ANALISIS CONSISTE EN: 1) INMOVILIZAR UN ANTICUERPO DE UN ANTIGENO ANFIPATICO (QUE HA DE SER DETECTADO O DETERMINADO) SOBRE UNA SUPERFICIE SOLIDA ACTIVADA PARA FORMAR UN INMUNOADSORBENTE; 2) FIJAR CUALQUIER ANTIGENO PRESENTE EN LA MUESTRA A LAS MEMBRANAS SUPERFICIALES DE LAS VESICULAS, LIPOSOMAS PREFERIBLEMENTE, LLENAS DE UN MARCADOR ENZIMATICO; 3) PONER EN CONTACTO LAS VESICULAS QUE CONTIENEN EL ANTIGENO CON EL INMUNOADSORBENTE PARA LIGAR CUALQUIER ANTIGENO CON EL ANTICUERPO; 4) LIBERAR EL MARCADOR ENZIMATICO PRODUCIENDO LA LISIS DE LA VESICULA; 5) MEDIR LA CANTIDAD DE MARCADOR ENZIMATICO. EL EQUIPO PARA TAL DETECCION CONSTA DE: A) VESICULAS LIPIDAS; B) MATERIAL SOLIDO DE SOPORTE; C) AGENTE LITICO; D) SUSTRATO QUE REACCIONE CON LA ENZIMA PARA FORMAR UN PRODUCTO DETECTABLE. SE PRESENTAN ALTERNATIVAS ESPECIFICAS PARA CASOS PARTICULARES. N
ES487849A 1979-01-18 1980-01-18 Un metodo y su correspondiente equipo para la deteccion o determinacion de una incognita analizable. Expired ES8103174A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB7901866 1979-01-18

Publications (2)

Publication Number Publication Date
ES487849A0 ES487849A0 (es) 1981-02-16
ES8103174A1 true ES8103174A1 (es) 1981-02-16

Family

ID=10502595

Family Applications (1)

Application Number Title Priority Date Filing Date
ES487849A Expired ES8103174A1 (es) 1979-01-18 1980-01-18 Un metodo y su correspondiente equipo para la deteccion o determinacion de una incognita analizable.

Country Status (9)

Country Link
EP (1) EP0014530B1 (es)
JP (1) JPS56500030A (es)
AT (1) ATE6698T1 (es)
AU (2) AU5471780A (es)
DE (1) DE3066899D1 (es)
ES (1) ES8103174A1 (es)
GB (1) GB2051360B (es)
IE (1) IE50457B1 (es)
WO (1) WO1980001515A1 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342826A (en) * 1980-02-04 1982-08-03 Collaborative Research, Inc. Immunoassay products and methods
US4598051A (en) * 1980-03-12 1986-07-01 The Regents Of The University Of California Liposome conjugates and diagnostic methods therewith
US4806466A (en) * 1981-10-29 1989-02-21 The Regents Of The University Of California Cell agglutination reagent comprising conjugates of antibody covalently bound to liposomes
US4517303A (en) * 1982-10-20 1985-05-14 E. I. Du Pont De Nemours And Company Specific binding assays utilizing analyte-cytolysin conjugates
JPS5984162A (ja) * 1982-11-05 1984-05-15 Toshiba Corp 免疫分析法
EP0134222A1 (en) * 1982-12-23 1985-03-20 Cooper-Lipotech, Inc. Lipid-vesicle-surface assay reagent and method
FR2542871B1 (fr) * 1983-03-15 1986-05-16 Pasteur Institut Procede de dosage en phase heterogene de substances biologiques antigeniques, mettant en oeuvre des conjugues hybrides d'anticorps et reactifs pour sa mise en oeuvre
US4713324A (en) * 1983-09-01 1987-12-15 Technicon Instruments Corporation Inverted latency specific binding assay
US4703017C1 (en) * 1984-02-14 2001-12-04 Becton Dickinson Co Solid phase assay with visual readout
US4743560A (en) * 1984-03-26 1988-05-10 Becton Dickinson And Company Solid phase assay
US4708933A (en) * 1984-06-12 1987-11-24 Leaf Huang Immunoliposome assay-methods and products
US4957735A (en) * 1984-06-12 1990-09-18 The University Of Tennessee Research Corporation Target-sensitive immunoliposomes- preparation and characterization
US4666830A (en) * 1984-08-23 1987-05-19 Wagner Daniel B Assay employing sacs and sac lysing agent
US4752572A (en) * 1985-08-30 1988-06-21 Eastman Kodak Company Lipid vesicles containing labeled species and their analytical uses
US4937188A (en) * 1986-04-15 1990-06-26 Northeastern University Enzyme activity amplification method for increasing assay sensitivity
US5190864A (en) * 1986-04-15 1993-03-02 Northeastern University Enzyme amplification by using free enzyme to release enzyme from an immobilized enzyme material
US5096837A (en) * 1990-02-08 1992-03-17 Pacific Biotech, Inc. Immunochromatographic assay and method of using same
US5223220A (en) * 1990-03-27 1993-06-29 Pacific Biotech, Inc. Solid phase immunoassay device and method of making same
CA2074151A1 (en) * 1991-07-23 1993-01-24 Minoru Fujita Process for lysing liposome membrane
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
WO1994026299A1 (en) * 1993-05-13 1994-11-24 Becton Dickinson And Company Liposomes incorporating density media
US6306642B1 (en) 1997-11-24 2001-10-23 Quidel Corporation Enzyme substrate delivery and product registration in one step enzyme immunoassays
AU2003243348B2 (en) 2002-05-31 2009-12-03 Cornell Research Foundation, Inc. Universal biosensor and methods of use
EP4268800A1 (en) 2022-04-28 2023-11-01 Prescience Biotechnology Inc. Liposome comprising rapamycin or a derivative thereof and use thereof in therapy

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2322562C2 (de) * 1972-11-06 1984-03-29 Pharmacia AB, Uppsala Verfahren zur Bestimmung von Antigenen in einer Probe
US3887698A (en) * 1973-03-12 1975-06-03 Univ Leland Stanford Junior Sacs with epitopic sites on walls enclosing stable free radicals
FR2221731B1 (es) * 1973-03-12 1978-11-10 Univ Leland Stanford Junior
GB1470955A (en) * 1973-04-04 1977-04-21 Nat Res Dev Polymeric materials
FR2400204A1 (fr) * 1977-08-11 1979-03-09 Technical Research Affiliates Detection d'antigenes au moyen de particules microbiennes sensiblisees par un anticorps

Also Published As

Publication number Publication date
IE800100L (en) 1980-07-18
AU5471780A (en) 1980-07-24
ES487849A0 (es) 1981-02-16
DE3066899D1 (en) 1984-04-19
ATE6698T1 (de) 1984-03-15
EP0014530B1 (en) 1984-03-14
AU3466084A (en) 1985-03-14
WO1980001515A1 (en) 1980-07-24
GB2051360A (en) 1981-01-14
GB2051360B (en) 1983-05-18
IE50457B1 (en) 1986-04-30
JPS56500030A (es) 1981-01-08
EP0014530A1 (en) 1980-08-20

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000301