KR910001379A - 핵산 결정 방법 - Google Patents

핵산 결정 방법 Download PDF

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Publication number
KR910001379A
KR910001379A KR1019900008705A KR900008705A KR910001379A KR 910001379 A KR910001379 A KR 910001379A KR 1019900008705 A KR1019900008705 A KR 1019900008705A KR 900008705 A KR900008705 A KR 900008705A KR 910001379 A KR910001379 A KR 910001379A
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KR
South Korea
Prior art keywords
nucleic acid
biological particles
test solution
virus
carrier
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KR1019900008705A
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English (en)
Inventor
요하네스 마리아리욘트 에스 페트러스
플러스 클레멘스 쿠요퍼스 네오나르더스
아놀드 헤이요팅크 루돌프
Original Assignee
에프. 지. 엠 헤르만스 피. 씨. 숄크비요크
악조 엔. 브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 에프. 지. 엠 헤르만스 피. 씨. 숄크비요크, 악조 엔. 브이. filed Critical 에프. 지. 엠 헤르만스 피. 씨. 숄크비요크
Publication of KR910001379A publication Critical patent/KR910001379A/ko

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • G01N33/5761—Hepatitis B
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Communicable Diseases (AREA)
  • Medicinal Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

HIV p24 정량 패널
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (5)

  1. 시험액의 핵산을 정성적 및/또는 정량적으로 결정하는 방법에 있어서, 시험액을 면역화학적 성분이 제공된 고형 캐리어와 항은 처리하여 면역 호학 반응에 의해 고형캐리어를 검출될 핵산을 함유하는 상기 시험액속의 생물학적 입자와 결합시킨후, 캐리어에 결합된 생물학적 입자를 시험액에서 분리한 다음, 캐리어에 결합된 생물학적 입자를 제제와 항온 처리하여 생물학적 입자를 개방시켜 핵산을 방출하게 하고, 동시 또는 후속적으로, 방출된 핵산을 증폭시킨후 핵산의 존재 유무 및/또는 양을 결정하여 시험액에서의 생물학적 입자의 존재 유무 및/또는 양을 측정하는 척도를 제공하는 것을 특징으로 하는 방법.
  2. 제1항에 있어서, 상기 생물학적 입자가 단백질을 함유하는 외피(coat)를 가지며 상기 고형 캐리어에는 상기 생물학적 입자 외피의 에피토프에 지향된 단일 클론성 항체가 제공되는 것을 특징으로 하는 방법.
  3. 제2항에 있어서, 생물학적 입자가 바이러스이고, 캐리어에 결합된 단일 클론성 항체가 이 바이러스의 에피토프에 지향된 것을 특징으로 하는 방법
  4. 제3항에 있어서, 상기 바이러스가 간염 B 바이러스이고 상기 단일클론성 항체가 항-preSl 단일클론성 항체인 방법.
  5. 제1항에 따른 결정과정을 수행하는 시험용 키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900008705A 1989-06-15 1990-06-14 핵산 결정 방법 KR910001379A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8901512 1989-06-15
NL8901512 1989-06-15

Publications (1)

Publication Number Publication Date
KR910001379A true KR910001379A (ko) 1991-01-30

Family

ID=19854838

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900008705A KR910001379A (ko) 1989-06-15 1990-06-14 핵산 결정 방법

Country Status (12)

Country Link
EP (1) EP0402997B1 (ko)
JP (1) JPH0333656A (ko)
KR (1) KR910001379A (ko)
AT (1) ATE111533T1 (ko)
AU (1) AU641399B2 (ko)
CA (1) CA2018877A1 (ko)
DE (1) DE69012440T2 (ko)
DK (1) DK0402997T3 (ko)
ES (1) ES2063902T3 (ko)
FI (1) FI902934A0 (ko)
IE (1) IE64040B1 (ko)
ZA (1) ZA904600B (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100469568B1 (ko) * 2001-12-18 2005-02-02 한국전자통신연구원 버퍼 모니터링을 통한 오디오 잡음 감쇄 제어 장치 및 그방법

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9002696A (nl) * 1990-11-15 1992-06-01 U Gene Research Bv Werkwijze en kit voor het aantonen van microoerganismen.
EP0488243A1 (en) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Method of extracting virus genome from sample derived from living body infected with the virus and detecting the genome
FR2674632B1 (fr) * 1991-03-29 1994-07-22 Bertin & Cie Procede et dispositif de detection rapide de micro-organismes indesirables dans un produit du domaine agro-alimentaire ou medico-veterinaire.
GB9107124D0 (en) * 1991-04-05 1991-05-22 Dynal As Chemical process
CA2127883A1 (en) * 1992-01-13 1993-07-22 Denis R. Henrard A method for amplifying and detecting a target nucleic acid sequence of hiv-1
GB9208000D0 (en) * 1992-04-10 1992-05-27 Univ London Quantitative viral assay
ES2044784B1 (es) * 1992-06-12 1994-09-01 Inia Procedimiento para la deteccion e identificacion de patogenos virales y subvirales.
ES2074961B1 (es) * 1993-12-21 1996-04-01 Inia Procedimiento para analizar estructuralmente el genoma de patogenos virales y subvirales.
ES2121698B1 (es) * 1996-12-02 1999-07-01 Inia Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method
EP1002138B1 (en) * 1997-08-08 2004-11-17 Organon Teknika B.V. Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of hiv-1
FR2810049A1 (fr) * 2000-06-09 2001-12-14 Inst Nat Sante Rech Med Methode de detection et/ou de quantification de variants minoritaires au sein d'une population virale heterogene dans un echantillon biologique
ES2182667B1 (es) * 2000-12-01 2004-06-01 Universidade Da Coruña Metodo universal de extraccion de adn de alta calidad.
GB0304832D0 (en) * 2003-03-04 2003-04-09 Secr Defence Assay method
WO2008156135A1 (ja) * 2007-06-20 2008-12-24 Toyohashi University Of Technology 核酸を有する微粒子の計測方法および装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4526690A (en) * 1983-02-04 1985-07-02 Millipore Corporation Apparatus for nucleic acid quantification
GB8331071D0 (en) * 1983-11-22 1983-12-29 Karayiannis P Assay for dna/rna
AU606043B2 (en) * 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
NO870614L (no) * 1986-03-06 1987-09-07 Molecular Diagnostics Inc Rask dna-pŸvisning av mikrober.
US5077192A (en) * 1988-10-25 1991-12-31 The General Hospital Corporation Method of detecting antigenic, nucleic acid-containing macromolecular entities

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100469568B1 (ko) * 2001-12-18 2005-02-02 한국전자통신연구원 버퍼 모니터링을 통한 오디오 잡음 감쇄 제어 장치 및 그방법

Also Published As

Publication number Publication date
CA2018877A1 (en) 1990-12-15
EP0402997B1 (en) 1994-09-14
AU641399B2 (en) 1993-09-23
FI902934A0 (fi) 1990-06-12
DE69012440T2 (de) 1995-02-09
IE902029L (en) 1990-12-15
IE64040B1 (en) 1995-06-28
EP0402997A3 (en) 1991-12-04
DK0402997T3 (da) 1994-10-31
ZA904600B (en) 1991-05-29
ES2063902T3 (es) 1995-01-16
EP0402997A2 (en) 1990-12-19
AU5716090A (en) 1990-12-20
JPH0333656A (ja) 1991-02-13
ATE111533T1 (de) 1994-09-15
DE69012440D1 (de) 1994-10-20

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