ES2074961B1 - Procedimiento para analizar estructuralmente el genoma de patogenos virales y subvirales. - Google Patents

Procedimiento para analizar estructuralmente el genoma de patogenos virales y subvirales.

Info

Publication number
ES2074961B1
ES2074961B1 ES09302657A ES9302657A ES2074961B1 ES 2074961 B1 ES2074961 B1 ES 2074961B1 ES 09302657 A ES09302657 A ES 09302657A ES 9302657 A ES9302657 A ES 9302657A ES 2074961 B1 ES2074961 B1 ES 2074961B1
Authority
ES
Spain
Prior art keywords
genome
procedure
pathogens
viral
immobilization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES09302657A
Other languages
English (en)
Other versions
ES2074961A1 (es
Inventor
Barbosa Gustavo Nuno
David Martinez
Flora Sanchez
Javier Romero
Fernando Ponz
Pascual Vicente Torres
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Original Assignee
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA filed Critical Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority to ES09302657A priority Critical patent/ES2074961B1/es
Priority to EP94500206A priority patent/EP0663448A1/en
Priority to CA002143150A priority patent/CA2143150A1/en
Priority to JP7073121A priority patent/JPH08294398A/ja
Publication of ES2074961A1 publication Critical patent/ES2074961A1/es
Application granted granted Critical
Publication of ES2074961B1 publication Critical patent/ES2074961B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

PROCEDIMIENTO PARA ANALIZAR ESTRUCTURALMENTE EL GENOMA DE PATOGENOS VIRALES Y SUBVIRALES. LA PRESENTE INVENCION SE REFIERE A UN PROCEDIMIENTO PARA ANALIZAR ESTRUCTURALMENTE EL GENOMA DE PATOGENOS VIRALES Y SUBVIRALES. EL PROCEDIMIENTO PARTE DE LA INMOVILIZACION SOBRE UNA FASE SOLIDA DE ESTRUCTURAS MACROMOLECULARES RELACIONADAS CON EL PATOGENO. TRAS LA INMOVILIZACION DEL PATOGENO SE PROCEDE A LA AMPLIFICACION ENZIMATICA DE UN FRAGMENTO DE SU GENOMA EN EL CASO DE PATOGENOS CON GENOMA DNA. CUANDO SE TRATE DE PATOGENOS CON GENOMA RNA, DESPUES DE LA INMOVILIZACION Y ANTES DE LA AMPLIFICACION ENZIMATICA, SE LLEVA A CABO LA TRANSCRIPCION INVERSA DE UN FRAGMENTO DEL GENOMA DEL PATOGENO. EL PROCEDIMIENTO SE TERMINA REALIZANDO UN ESTUDIO DE LA ESTRUCTURA DE LOS PRODUCTOS RESULTANTES DE LA ETAPA DE AMPLIFICACION ENZIMATICA.
ES09302657A 1993-12-21 1993-12-21 Procedimiento para analizar estructuralmente el genoma de patogenos virales y subvirales. Expired - Fee Related ES2074961B1 (es)

Priority Applications (4)

Application Number Priority Date Filing Date Title
ES09302657A ES2074961B1 (es) 1993-12-21 1993-12-21 Procedimiento para analizar estructuralmente el genoma de patogenos virales y subvirales.
EP94500206A EP0663448A1 (en) 1993-12-21 1994-12-20 Process for structurally analyzing the genome of viral and subviral pathogens
CA002143150A CA2143150A1 (en) 1993-12-21 1995-02-22 Process for structurally analyzing the genome of viral and subviral pathogens
JP7073121A JPH08294398A (ja) 1993-12-21 1995-03-30 ウイルス性又はサブウイルス性の病原体のゲノムの構造分析方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ES09302657A ES2074961B1 (es) 1993-12-21 1993-12-21 Procedimiento para analizar estructuralmente el genoma de patogenos virales y subvirales.
CA002143150A CA2143150A1 (en) 1993-12-21 1995-02-22 Process for structurally analyzing the genome of viral and subviral pathogens
JP7073121A JPH08294398A (ja) 1993-12-21 1995-03-30 ウイルス性又はサブウイルス性の病原体のゲノムの構造分析方法

Publications (2)

Publication Number Publication Date
ES2074961A1 ES2074961A1 (es) 1995-09-16
ES2074961B1 true ES2074961B1 (es) 1996-04-01

Family

ID=27169963

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09302657A Expired - Fee Related ES2074961B1 (es) 1993-12-21 1993-12-21 Procedimiento para analizar estructuralmente el genoma de patogenos virales y subvirales.

Country Status (4)

Country Link
EP (1) EP0663448A1 (es)
JP (1) JPH08294398A (es)
CA (1) CA2143150A1 (es)
ES (1) ES2074961B1 (es)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5077192A (en) * 1988-10-25 1991-12-31 The General Hospital Corporation Method of detecting antigenic, nucleic acid-containing macromolecular entities
IE64040B1 (en) * 1989-06-15 1995-06-28 Akzo Nv Method for determining nucleic acid
WO1991011534A1 (en) * 1990-01-24 1991-08-08 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce A rapid and sensitive test for detecting hepatitis a virus
ES2044784B1 (es) * 1992-06-12 1994-09-01 Inia Procedimiento para la deteccion e identificacion de patogenos virales y subvirales.

Also Published As

Publication number Publication date
CA2143150A1 (en) 1996-08-23
EP0663448A1 (en) 1995-07-19
JPH08294398A (ja) 1996-11-12
ES2074961A1 (es) 1995-09-16

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20071222