ES451047A1 - Procedimiento de separacion de proteinas. - Google Patents

Procedimiento de separacion de proteinas.

Info

Publication number
ES451047A1
ES451047A1 ES451047A ES451047A ES451047A1 ES 451047 A1 ES451047 A1 ES 451047A1 ES 451047 A ES451047 A ES 451047A ES 451047 A ES451047 A ES 451047A ES 451047 A1 ES451047 A1 ES 451047A1
Authority
ES
Spain
Prior art keywords
ion exchange
separating proteins
proteins
exchanging groups
separating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES451047A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rhone Poulenc Industries SA
Original Assignee
Rhone Poulenc Industries SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR7526530A external-priority patent/FR2321932A1/fr
Priority claimed from FR7622985A external-priority patent/FR2359634A2/fr
Application filed by Rhone Poulenc Industries SA filed Critical Rhone Poulenc Industries SA
Publication of ES451047A1 publication Critical patent/ES451047A1/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462Lysozyme (3.2.1.17)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/14Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment
    • A23C9/146Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by ion-exchange
    • A23C9/1465Chromatographic separation of protein or lactose fraction; Adsorption of protein or lactose fraction followed by elution
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/001Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/08Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from eggs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/16Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste water of starch-manufacturing plant or like wastes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00Ion-exchange processes in general; Apparatus therefor
    • B01J47/014Ion-exchange processes in general; Apparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00Ion-exchange processes in general; Apparatus therefor
    • B01J47/016Modification or after-treatment of ion-exchangers
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065Purification, fragmentation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12HPASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/02Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material
    • C12H1/04Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material
    • C12H1/0432Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material with the aid of ion-exchange material or inert clarification material, e.g. adsorption material with the aid of ion-exchange material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S526/00Synthetic resins or natural rubbers -- part of the class 520 series
    • Y10S526/936Physiological use, e.g. pharmaceutical, veterinary, dental
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827Proteins from mammals or birds
    • Y10S530/829Blood
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827Proteins from mammals or birds
    • Y10S530/832Milk; colostrum
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/859Waste, waste material, refuse or sludge, e.g. effluents, fecal matter
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00Stock material or miscellaneous articles
    • Y10T428/29Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991Coated
    • Y10T428/2998Coated including synthetic resin or polymer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Filtering Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Procedimiento de separación de proteínas, caracterizado porque comprende las operaciones siguientes: a) Puesta en contacto a una temperatura, bajo una fuerza ionizante y un pH compatibles con la o las proteínas, de una solución de proteínas con una resina intercambiadora de iones, constituida por un soporte mineral poroso que tiene una granulometría comprendida entre 4 um y 5mm, una superficie específica del orden de 5 a 150 m2/gr, un diámetro de poro comprendido entre 500 y 2.500 A, un volumen poroso de 0,4 a 2 ml/g revestido con una cantidad inferior a 15 mg/m2 de una película de polímero reticulado que contiene o es portador, bien de grupos intercambiadores de aniones, representados por aminas terciarias o sales de amonio cuaternario, bien agrupamientos intercambiadores de cationes representados por funciones ácidas, y que posee una capacidad de intercambio inferior a 2 meg/g; b) fijación sobre la resina intercambiadora de iones de una o varias proteínas; c) elusión de las proteínas fijadas con una solución que tenga un pH y/o una fuerza iónica diferentes a las de la solución a tratar.
ES451047A 1975-08-28 1976-08-27 Procedimiento de separacion de proteinas. Expired ES451047A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR7526530A FR2321932A1 (fr) 1975-08-28 1975-08-28 Procede de separation de proteines par echange d'ions
FR7622985A FR2359634A2 (fr) 1976-07-28 1976-07-28 Procede de separation de proteines par echange d'ions

Publications (1)

Publication Number Publication Date
ES451047A1 true ES451047A1 (es) 1978-05-01

Family

ID=26219047

Family Applications (1)

Application Number Title Priority Date Filing Date
ES451047A Expired ES451047A1 (es) 1975-08-28 1976-08-27 Procedimiento de separacion de proteinas.

Country Status (21)

Country Link
US (1) US4100149A (es)
JP (1) JPS5257200A (es)
AR (1) AR209193A1 (es)
AU (1) AU500134B2 (es)
BR (1) BR7605661A (es)
CA (1) CA1069843A (es)
CH (1) CH602780A5 (es)
DE (1) DE2638764C3 (es)
DK (1) DK156577C (es)
ES (1) ES451047A1 (es)
GB (1) GB1513195A (es)
IE (1) IE43536B1 (es)
IT (1) IT1066221B (es)
MX (1) MX4333E (es)
NL (1) NL178294C (es)
NO (1) NO145508C (es)
NZ (1) NZ181884A (es)
RO (1) RO78229A (es)
SE (1) SE426911B (es)
SU (1) SU688124A3 (es)
YU (1) YU209676A (es)

Families Citing this family (60)

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US4326008A (en) * 1976-08-27 1982-04-20 California Institute Of Technology Protein specific fluorescent microspheres for labelling a protein
US4229342A (en) * 1977-05-18 1980-10-21 Rhone-Poulenc Industries Process for extracting proteins from milk using silica and anion exchange resins
GB1573684A (en) * 1977-07-04 1980-08-28 Trinchera C Process for the production of lysozyme
US4171204A (en) * 1978-09-01 1979-10-16 Abbott Laboratories Precipitation of protein
FR2439791A1 (fr) * 1978-10-27 1980-05-23 Rhone Poulenc Ind Procede de purification de proteines vegetales
JPS5598117A (en) * 1979-01-17 1980-07-25 Chemo Sero Therapeut Res Inst Purification of gamma-globulin derivative
US4305870A (en) * 1979-01-31 1981-12-15 Immuno Aktiengesellschaft Fur Chemisch-Medizinische Produkte Intravenous plasma derivatives and their production
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JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンW R Grace & Co−Conn 官能化担体材料並びに官能化担体材料を作製及び使用する方法
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US3557082A (en) * 1969-01-24 1971-01-19 Tee Pak Inc Process for separating ionic materials from component mixtures
DE2319581C2 (de) * 1973-04-18 1975-03-27 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur chromatographischen Fraktionierung von Molke
FR2236552B1 (es) * 1973-07-13 1977-05-06 Rhone Progil
JPS5426396B2 (es) * 1974-06-04 1979-09-04

Also Published As

Publication number Publication date
CA1069843A (en) 1980-01-15
NO145508B (no) 1981-12-28
IE43536B1 (en) 1981-03-25
AU500134B2 (en) 1979-05-10
YU209676A (en) 1982-10-31
DK389176A (da) 1977-03-01
AR209193A1 (es) 1977-03-31
JPS5257200A (en) 1977-05-11
AU1721776A (en) 1978-03-02
NL178294C (nl) 1986-03-03
IE43536L (en) 1977-02-28
SU688124A3 (ru) 1979-09-25
RO78229A (ro) 1982-02-26
DK156577C (da) 1990-01-29
DK156577B (da) 1989-09-11
US4100149A (en) 1978-07-11
GB1513195A (en) 1978-06-07
NL7609562A (nl) 1977-03-02
NZ181884A (en) 1978-04-28
DE2638764B2 (de) 1978-09-14
NO145508C (no) 1982-04-14
IT1066221B (it) 1985-03-04
DE2638764C3 (de) 1981-02-12
DE2638764A1 (de) 1977-03-03
JPS5715840B2 (es) 1982-04-01
SE426911B (sv) 1983-02-21
SE7609479L (sv) 1977-03-01
BR7605661A (pt) 1978-03-21
MX4333E (es) 1982-03-26
NO762959L (es) 1977-03-01
CH602780A5 (es) 1978-07-31

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