ES2930344T3 - Aislamiento de micro ARN a partir de fluido biológico - Google Patents

Aislamiento de micro ARN a partir de fluido biológico Download PDF

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Publication number
ES2930344T3
ES2930344T3 ES20152748T ES20152748T ES2930344T3 ES 2930344 T3 ES2930344 T3 ES 2930344T3 ES 20152748 T ES20152748 T ES 20152748T ES 20152748 T ES20152748 T ES 20152748T ES 2930344 T3 ES2930344 T3 ES 2930344T3
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ES
Spain
Prior art keywords
mirna
sodium
surfactant
plasma
binding protein
Prior art date
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Active
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ES20152748T
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English (en)
Spanish (es)
Inventor
Carol Kreader
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Sigma-Aldrich Co LLC
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Sigma-Aldrich Co LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21014—Microbial serine proteases (3.4.21.14)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
ES20152748T 2013-11-27 2014-11-25 Aislamiento de micro ARN a partir de fluido biológico Active ES2930344T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361909834P 2013-11-27 2013-11-27
US201461985000P 2014-04-28 2014-04-28

Publications (1)

Publication Number Publication Date
ES2930344T3 true ES2930344T3 (es) 2022-12-12

Family

ID=53403495

Family Applications (2)

Application Number Title Priority Date Filing Date
ES20152748T Active ES2930344T3 (es) 2013-11-27 2014-11-25 Aislamiento de micro ARN a partir de fluido biológico
ES14872184T Active ES2778223T3 (es) 2013-11-27 2014-11-25 Aislamiento de ARNmicro de fluido biológico

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES14872184T Active ES2778223T3 (es) 2013-11-27 2014-11-25 Aislamiento de ARNmicro de fluido biológico

Country Status (10)

Country Link
US (2) US10131902B2 (enExample)
EP (2) EP3702361B1 (enExample)
JP (1) JP6644686B2 (enExample)
KR (1) KR102181103B1 (enExample)
CN (2) CN110511924B (enExample)
BR (1) BR112016011770B1 (enExample)
CA (1) CA2930704C (enExample)
ES (2) ES2930344T3 (enExample)
IL (1) IL245646B (enExample)
WO (1) WO2015094609A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112016011770B1 (pt) 2013-11-27 2022-10-25 Sigma-Aldrich Co. Llc Método e kit para isolamento de microrna a partir de amostra biológica
CN107385014B (zh) * 2017-06-13 2020-12-22 深圳大学 一种直接定量检测循环miRNA的RT-qPCR方法
KR102003594B1 (ko) 2017-08-08 2019-07-24 숙명여자대학교산학협력단 염증 반응 매개 신혈관생성 질환 진단용 조성물
CN111278844B (zh) 2017-09-13 2023-12-19 贝克顿·迪金森公司 用于使用氧化铁颗粒提取核酸的方法和组合物
CA3094752A1 (en) 2018-03-22 2019-09-26 Idexx Laboratories, Inc. Methods for measuring bacteria in biological samples
CN109490533B (zh) * 2018-12-29 2022-02-15 南京澳林生物科技有限公司 一种胃蛋白酶原ⅱ(pgⅱ)检测试剂盒及生产工艺
US20240312562A1 (en) * 2020-10-26 2024-09-19 Eclipse Bioinnovations, Inc. Methods and kits for enriching for polynucleotides
US20240318163A1 (en) * 2021-07-08 2024-09-26 National Institute Of Advanced Industrial Science And Technology Method for noninvasively acquiring micro-rna from cells
WO2023023584A2 (en) 2021-08-19 2023-02-23 Eclipse Bioinnovations, Inc. Methods for detecting rna binding protein complexes
CN113980954A (zh) * 2021-11-11 2022-01-28 德州学院 一种病毒rna保护剂及其制备方法与应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050142609A1 (en) * 2003-10-08 2005-06-30 Ebioscience Native immunoglobulin binding reagents and methods for making and using same
JP5349838B2 (ja) * 2007-11-30 2013-11-20 和光純薬工業株式会社 ｓｍａｌｌＲＮＡの取得用担体、取得方法及び取得用試薬
US8841073B2 (en) * 2008-07-28 2014-09-23 The Rockefeller University Methods for identifying RNA segments bound by RNA-binding proteins or ribonucleoprotein complexes
US8846314B2 (en) * 2009-03-03 2014-09-30 The Board Of Trustees Of The Leland Stanford Junior University Isotachophoretic focusing of nucleic acids
WO2012039395A1 (ja) * 2010-09-24 2012-03-29 和光純薬工業株式会社 small RNAの取得方法
JP2014507160A (ja) * 2011-02-22 2014-03-27 カリス ライフ サイエンシズ ルクセンブルク ホールディングス エス．アー．エール．エル． 循環バイオマーカー
ES2632212T3 (es) * 2011-08-04 2017-09-11 Yeda Research And Development Co. Ltd. miR-135 y composiciones que lo comprenden para el tratamiento de afecciones médicas asociadas con la serotonina
CN102628038A (zh) * 2012-04-09 2012-08-08 武汉生命之美科技有限公司 一种改进的clip法鉴定rna结合蛋白的靶标rna的方法
CA2892490A1 (en) * 2012-11-26 2014-05-30 Caris Science, Inc. Biomarker compositions and methods
EP3027617B1 (en) * 2013-07-31 2026-02-18 Ionis Pharmaceuticals, Inc. Methods and compounds useful in conditions related to repeat expansion
BR112016011770B1 (pt) 2013-11-27 2022-10-25 Sigma-Aldrich Co. Llc Método e kit para isolamento de microrna a partir de amostra biológica
US20160289734A1 (en) * 2015-04-03 2016-10-06 University Of Massachusetts Methods of using oligonucleotide-guided argonaute proteins

Also Published As

Publication number Publication date
EP3074413B1 (en) 2020-01-22
WO2015094609A1 (en) 2015-06-25
JP2017501689A (ja) 2017-01-19
CA2930704C (en) 2023-02-21
CN105934439A (zh) 2016-09-07
ES2778223T3 (es) 2020-08-10
EP3702361B1 (en) 2022-08-31
EP3702361A1 (en) 2020-09-02
BR112016011770B1 (pt) 2022-10-25
CA2930704A1 (en) 2015-06-25
EP3074413A1 (en) 2016-10-05
IL245646B (en) 2018-06-28
KR102181103B1 (ko) 2020-11-23
IL245646A0 (en) 2016-06-30
KR20160089398A (ko) 2016-07-27
US10711266B2 (en) 2020-07-14
CN105934439B (zh) 2019-09-10
JP6644686B2 (ja) 2020-02-12
CN110511924A (zh) 2019-11-29
CN110511924B (zh) 2023-11-03
US10131902B2 (en) 2018-11-20
US20160376582A1 (en) 2016-12-29
BR112016011770A2 (enExample) 2017-08-08
US20190144850A1 (en) 2019-05-16
EP3074413A4 (en) 2017-05-31

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