ES2900331T3 - Elementos promotores y reguladores para mejorar la expresión de genes heterólogos en células hospederas - Google Patents
Elementos promotores y reguladores para mejorar la expresión de genes heterólogos en células hospederas Download PDFInfo
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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| PCT/US2015/055549 WO2016061240A1 (en) | 2014-10-15 | 2015-10-14 | Promoter and regulatory elements for improved expression of heterologous genes in host cells |
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| EP4218799A1 (en) | 2009-07-15 | 2023-08-02 | GlaxoSmithKline Biologicals S.A. | Rsv f protein compositions and methods for making same |
| DK2707385T3 (da) | 2011-05-13 | 2017-11-20 | Glaxosmithkline Biologicals Sa | RSV-F-præfusionsantigener |
| TW202233660A (zh) | 2020-10-30 | 2022-09-01 | 美商安進公司 | 過表現胰島素樣生長因子受體突變體以調節ｉｇｆ補充 |
| CN114196703B (zh) * | 2021-12-22 | 2023-02-03 | 西北农林科技大学 | 一种提高牦牛克隆胚胎发育率的载体、细胞和方法 |
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| US4657866A (en) | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| GB8516415D0 (en) | 1985-06-28 | 1985-07-31 | Celltech Ltd | Culture of animal cells |
| US4927762A (en) | 1986-04-01 | 1990-05-22 | Cell Enterprises, Inc. | Cell culture medium with antioxidant |
| US5567610A (en) | 1986-09-04 | 1996-10-22 | Bioinvent International Ab | Method of producing human monoclonal antibodies and kit therefor |
| WO1990001063A1 (en) | 1988-07-23 | 1990-02-08 | Delta Biotechnology Limited | New secretory leader sequences |
| AU632065B2 (en) | 1988-09-23 | 1992-12-17 | Novartis Vaccines And Diagnostics, Inc. | Cell culture medium for enhanced cell growth, culture longevity and product expression |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| US5175384A (en) | 1988-12-05 | 1992-12-29 | Genpharm International | Transgenic mice depleted in mature t-cells and methods for making transgenic mice |
| EP1690934A3 (en) | 1990-01-12 | 2008-07-30 | Abgenix, Inc. | Generation of xenogeneic antibodies |
| US5229275A (en) | 1990-04-26 | 1993-07-20 | Akzo N.V. | In-vitro method for producing antigen-specific human monoclonal antibodies |
| US5427908A (en) | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
| GB9014932D0 (en) | 1990-07-05 | 1990-08-22 | Celltech Ltd | Recombinant dna product and method |
| GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| GB9022543D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Antibody production |
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| ES2136092T3 (es) | 1991-09-23 | 1999-11-16 | Medical Res Council | Procedimientos para la produccion de anticuerpos humanizados. |
| ATE244763T1 (de) | 1992-02-11 | 2003-07-15 | Cell Genesys Inc | Erzielen von homozygotem durch zielgerichtete genetische ereignisse |
| US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
| NZ255101A (en) | 1992-07-24 | 1997-08-22 | Cell Genesys Inc | A yeast artificial chromosome (yac) vector containing an hprt minigene expressible in murine stem cells and genetically modified rodent therefor |
| US6054287A (en) | 1994-05-27 | 2000-04-25 | Methodist Hospital Of Indiana, Inc. | Cell-type-specific methods and devices for the low temperature preservation of the cells of an animal species |
| US6130364A (en) | 1995-03-29 | 2000-10-10 | Abgenix, Inc. | Production of antibodies using Cre-mediated site-specific recombination |
| CA2219361C (en) | 1995-04-27 | 2012-02-28 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| GB9712818D0 (en) | 1996-07-08 | 1997-08-20 | Cambridge Antibody Tech | Labelling and selection of specific binding molecules |
| EP1500329B1 (en) | 1996-12-03 | 2012-03-21 | Amgen Fremont Inc. | Human antibodies that specifically bind human TNF alpha |
| US20020032315A1 (en) | 1997-08-06 | 2002-03-14 | Manuel Baca | Anti-vegf antibodies |
| US20030044772A1 (en) | 1997-08-04 | 2003-03-06 | Applied Molecular Evolution [Formerly Ixsys] | Methods for identifying ligand specific binding molecules |
| US6022952A (en) | 1998-04-01 | 2000-02-08 | University Of Alberta | Compositions and methods for protein secretion |
| US20020029391A1 (en) | 1998-04-15 | 2002-03-07 | Claude Geoffrey Davis | Epitope-driven human antibody production and gene expression profiling |
| US6210924B1 (en) | 1998-08-11 | 2001-04-03 | Amgen Inc. | Overexpressing cyclin D 1 in a eukaryotic cell line |
| US6660843B1 (en) | 1998-10-23 | 2003-12-09 | Amgen Inc. | Modified peptides as therapeutic agents |
| CA2350226C (en) | 1998-11-20 | 2012-04-24 | Fuso Pharmaceutical Industries, Ltd. | Protein expression vector and utilization thereof |
| JP2002538841A (ja) | 1999-03-18 | 2002-11-19 | ヒューマン ジノーム サイエンシーズ， インコーポレイテッド | ２７個のヒト分泌タンパク質 |
| CN1487996B (zh) | 2000-11-30 | 2010-06-16 | 米德列斯公司 | 用于生产人类抗体的转基因转染色体啮齿动物 |
| US20040257396A1 (en) | 2003-06-19 | 2004-12-23 | Toshiba Tec Kabushiki Kaisha | Ink jet head cleaning apparatus and ink jet recording apparatus |
| GB0509965D0 (en) * | 2005-05-17 | 2005-06-22 | Ml Lab Plc | Improved expression elements |
| TW200722436A (en) | 2005-10-21 | 2007-06-16 | Hoffmann La Roche | A peptide-immunoglobulin-conjugate |
| JP2010525789A (ja) * | 2007-01-08 | 2010-07-29 | ミリポア・コーポレイション | １つ又はそれ以上のきょう雑物の低下したレベルの存在下で組換えタンパク質を生産するための細胞培養法 |
| EP2788483B1 (en) * | 2011-12-07 | 2018-06-27 | Glenmark Pharmaceuticals S.A. | Expression cassette |
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- 2015-10-14 AU AU2015332577A patent/AU2015332577B2/en active Active
- 2015-10-14 ES ES20173389T patent/ES2962385T3/es active Active
- 2015-10-14 US US15/519,820 patent/US10202616B2/en active Active
- 2015-10-14 EP EP20173389.6A patent/EP3722433B1/en active Active
- 2015-10-14 JP JP2017520418A patent/JP6749898B2/ja active Active
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| Publication number | Publication date |
|---|---|
| EP3722433A1 (en) | 2020-10-14 |
| AU2015332577B2 (en) | 2021-12-23 |
| EP3207146B1 (en) | 2021-09-29 |
| EP3207146A1 (en) | 2017-08-23 |
| AU2015332577A1 (en) | 2017-05-04 |
| CA2964313A1 (en) | 2016-04-21 |
| JP6749898B2 (ja) | 2020-09-02 |
| ES2962385T3 (es) | 2024-03-18 |
| WO2016061240A1 (en) | 2016-04-21 |
| US10202616B2 (en) | 2019-02-12 |
| MX2017004769A (es) | 2017-10-16 |
| WO2016061240A8 (en) | 2017-05-18 |
| CA2964313C (en) | 2023-03-14 |
| EP3722433B1 (en) | 2023-07-26 |
| US20170253889A1 (en) | 2017-09-07 |
| MX377591B (es) | 2025-03-10 |
| JP2017530718A (ja) | 2017-10-19 |
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