ES2877367T3 - Elementos reguladores postranscripcionales de hepatitis modificados - Google Patents
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| WO2016073602A2 (en) | 2014-11-05 | 2016-05-12 | Juno Therapeutics, Inc. | Methods for transduction and cell processing |
| CA2994969A1 (en) | 2015-08-06 | 2017-02-09 | Dana-Farber Cancer Institute, Inc. | Targeted protein degradation to attenuate adoptive t-cell therapy associated adverse inflammatory responses |
| CN109641063B (zh) | 2016-04-20 | 2022-11-15 | 能源环境和技术研究中心O.A., M.P. | 用于增强pklr的基因表达的组合物和方法 |
| JP7042263B2 (ja) | 2016-10-20 | 2022-03-25 | サンガモ セラピューティクス， インコーポレイテッド | ファブリー病の治療のための方法および組成物 |
| CN107058315B (zh) * | 2016-12-08 | 2019-11-08 | 上海优卡迪生物医药科技有限公司 | 敲减人PD-1的siRNA、重组表达CAR-T载体及其构建方法和应用 |
| EP3580212A4 (en) | 2017-02-08 | 2021-03-17 | Dana Farber Cancer Institute, Inc. | REGULATION OF CHEMERIC ANTIGEN RECEPTORS |
| US11713468B2 (en) * | 2017-08-02 | 2023-08-01 | Dna Twopointo Inc. | DNA vectors and elements for sustained gene expression in eukaryotic cells |
| CA3076337A1 (en) | 2017-09-19 | 2019-03-28 | Massachusetts Institute Of Technology | Compositions for chimeric antigen receptor t cell therapy and uses thereof |
| CN111163810B (zh) | 2017-10-16 | 2024-09-10 | 能源环境和技术研究中心O.A.,M.P. | 用于递送pklr以治疗丙酮酸激酶缺乏症的慢病毒载体 |
| SG11202008400SA (en) | 2018-04-11 | 2020-09-29 | Rocket Pharmaceuticals Ltd | Compositions and methods for stem cell transplant |
| CA3096293A1 (en) | 2018-04-27 | 2019-10-31 | Rocket Pharmaceuticals, Ltd. | Gene therapy for cns degeneration |
| MX2021003435A (es) | 2018-09-28 | 2021-06-15 | Massachusetts Inst Technology | Moleculas inmunomoduladoras localizadas en el colageno y metodos de las mismas. |
| KR20210152515A (ko) | 2019-04-12 | 2021-12-15 | 씨4 테라퓨틱스, 인코포레이티드 | 이카로스 및 아이올로스의 트리시클릭 분해제 |
| CN111825769B (zh) * | 2019-04-16 | 2022-03-25 | 上海科技大学 | 一种泛素化缺失的嵌合抗原受体及其用途 |
| JP2022536692A (ja) | 2019-06-11 | 2022-08-18 | シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド | 調節不全の血漿カリクレインによって媒介される疾患の治療のための抗体のアデノ随伴ウイルスベクター送達 |
| US11642409B2 (en) | 2019-06-26 | 2023-05-09 | Massachusetts Insttute of Technology | Immunomodulatory fusion protein-metal hydroxide complexes and methods thereof |
| WO2021061648A1 (en) | 2019-09-23 | 2021-04-01 | Massachusetts Institute Of Technology | Methods and compositions for stimulation of endogenous t cell responses |
| CN115176022A (zh) | 2019-10-23 | 2022-10-11 | 夏尔人类遗传性治疗公司 | 用于遗传性血管性水肿的基于腺相关病毒载体的基因疗法 |
| IL296242A (en) | 2020-03-10 | 2022-11-01 | Massachusetts Inst Technology | Methods for producing engineered memory-like nk cells and preparations containing them |
| WO2021183207A1 (en) | 2020-03-10 | 2021-09-16 | Massachusetts Institute Of Technology | COMPOSITIONS AND METHODS FOR IMMUNOTHERAPY OF NPM1c-POSITIVE CANCER |
| DE102020106710A1 (de) | 2020-03-11 | 2021-09-16 | Immatics US, Inc. | Wpre-mutantenkonstrukte, zusammensetzungen und zugehörige verfahren |
| DE102020111571A1 (de) * | 2020-03-11 | 2021-09-16 | Immatics US, Inc. | Wpre-mutantenkonstrukte, zusammensetzungen und zugehörige verfahren |
| WO2021222133A1 (en) | 2020-04-27 | 2021-11-04 | Juno Therapeutics, Inc. | Stabilization of polyethyleneimine-deoxyribonucleic acid complex size and activity |
| US12433954B2 (en) | 2020-05-01 | 2025-10-07 | Massachusetts Institute Of Technology | Methods of activating anti-CD19 chimeric antigen receptor (CAR) T cells using amphiphilic ligand conjugates comprising CAR-targeting protein sequence motifs |
| US20210340524A1 (en) | 2020-05-01 | 2021-11-04 | Massachusetts Institute Of Technology | Methods for identifying chimeric antigen receptor-targeting ligands and uses thereof |
| EP4165196A1 (en) * | 2020-06-11 | 2023-04-19 | Sangamo Therapeutics, Inc. | Methods and compositions for expressing phenylalanine hydroxylase |
| AU2021300615A1 (en) * | 2020-06-30 | 2023-02-02 | The University Court Of The University Of Edinburgh | Transgene expression system |
| CA3185267A1 (en) | 2020-08-07 | 2022-02-10 | Spacecraft Seven, Llc | Plakophilin-2 (pkp2) gene therapy using aav vector |
| KR20220035693A (ko) * | 2020-09-14 | 2022-03-22 | 주식회사 제너로스 | 표적화된 유전자 전달을 위한 아데노-부속 바이러스 벡터 |
| US20220106614A1 (en) * | 2020-09-29 | 2022-04-07 | NeuExcell Therapeutics Inc. | Dlx2 vector |
| JP2023554066A (ja) | 2020-12-16 | 2023-12-26 | 武田薬品工業株式会社 | 血漿カリクレインの制御不全によって媒介される疾患の処置のための抗体のアデノ随伴ウイルスベクター送達 |
| UY39772A (es) * | 2021-05-21 | 2023-01-31 | Novartis Ag | Composiciones y métodos para mejorar la función visual |
| WO2023081715A1 (en) | 2021-11-03 | 2023-05-11 | Viracta Therapeutics, Inc. | Combination of car t-cell therapy with btk inhibitors and methods of use thereof |
| CA3236331A1 (en) * | 2021-11-04 | 2023-05-11 | Mayo Foundation For Medical Education And Research | Methods and materials for treating cancer |
| JP2025526237A (ja) | 2022-07-07 | 2025-08-13 | サイエンス コーポレイション | ニューラルインターフェースデバイス |
| WO2024077052A1 (en) * | 2022-10-04 | 2024-04-11 | Emergent Product Development Gaithersburg Inc. | Novel vsv virus formulations |
| WO2024226858A1 (en) | 2023-04-26 | 2024-10-31 | Juno Therapeutics, Inc. | Methods for viral vector manufacturing |
| WO2026003067A1 (en) * | 2024-06-25 | 2026-01-02 | Asgard Therapeutics Ab | Transcription factors and reprogramming modulators for reprogramming cells to cdc1 cells, compositions and methods thereof |
Family Cites Families (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4452773A (en) | 1982-04-05 | 1984-06-05 | Canadian Patents And Development Limited | Magnetic iron-dextran microspheres |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4690915A (en) | 1985-08-08 | 1987-09-01 | The United States Of America As Represented By The Department Of Health And Human Services | Adoptive immunotherapy as a treatment modality in humans |
| US4795698A (en) | 1985-10-04 | 1989-01-03 | Immunicon Corporation | Magnetic-polymer particles |
| ATE114507T1 (de) | 1988-12-28 | 1994-12-15 | Stefan Miltenyi | Verfahren sowie materialien zur hochgraduierten magnetischen abspaltung biologischer materialien. |
| US5200084A (en) | 1990-09-26 | 1993-04-06 | Immunicon Corporation | Apparatus and methods for magnetic separation |
| DK0557459T3 (da) | 1990-11-13 | 1997-12-15 | Immunex Corp | Bifunktionelle, selekterbare fusionsgener |
| AU6953394A (en) | 1993-05-21 | 1994-12-20 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| US5834256A (en) | 1993-06-11 | 1998-11-10 | Cell Genesys, Inc. | Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells |
| US5827642A (en) | 1994-08-31 | 1998-10-27 | Fred Hutchinson Cancer Research Center | Rapid expansion method ("REM") for in vitro propagation of T lymphocytes |
| US6013516A (en) | 1995-10-06 | 2000-01-11 | The Salk Institute For Biological Studies | Vector and method of use for nucleic acid delivery to non-dividing cells |
| WO1997034634A1 (en) | 1996-03-20 | 1997-09-25 | Sloan-Kettering Institute For Cancer Research | Single chain fv constructs of anti-ganglioside gd2 antibodies |
| US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| US6136597A (en) | 1997-09-18 | 2000-10-24 | The Salk Institute For Biological Studies | RNA export element |
| US5994136A (en) | 1997-12-12 | 1999-11-30 | Cell Genesys, Inc. | Method and means for producing high titer, safe, recombinant lentivirus vectors |
| FR2777909B1 (fr) | 1998-04-24 | 2002-08-02 | Pasteur Institut | Utilisation de sequences d'adn de structure triplex pour le tranfert de sequences de nucleotides dans des cellules, vecteurs recombinants contenant ces sequences triplex |
| JP2002524081A (ja) | 1998-09-04 | 2002-08-06 | スローン − ケッタリング インスティチュート フォー キャンサー リサーチ | 前立腺−特異的膜抗原に特異的な融合受容体およびその使用 |
| WO2000023573A2 (en) | 1998-10-20 | 2000-04-27 | City Of Hope | Cd20-specific redirected t cells and their use in cellular immunotherapy of cd20+ malignancies |
| US7262049B2 (en) | 1999-03-16 | 2007-08-28 | Dana-Farber Cancer Institute, Inc. | Pseudotyped lentiviral vectors and uses thereof |
| AU2001265346A1 (en) | 2000-06-02 | 2001-12-17 | Memorial Sloan-Kettering Cancer Center | Artificial antigen presenting cells and methods of use thereof |
| US7419829B2 (en) | 2000-10-06 | 2008-09-02 | Oxford Biomedica (Uk) Limited | Vector system |
| CA2425862C (en) | 2000-11-07 | 2013-01-22 | City Of Hope | Cd19-specific redirected immune cells |
| US7070995B2 (en) | 2001-04-11 | 2006-07-04 | City Of Hope | CE7-specific redirected immune cells |
| CA2344208A1 (en) | 2001-04-30 | 2002-10-30 | Oxford Biomedica (Uk) Limited | Method |
| US20090257994A1 (en) | 2001-04-30 | 2009-10-15 | City Of Hope | Chimeric immunoreceptor useful in treating human cancers |
| US20030170238A1 (en) | 2002-03-07 | 2003-09-11 | Gruenberg Micheal L. | Re-activated T-cells for adoptive immunotherapy |
| US7384738B2 (en) | 2002-03-28 | 2008-06-10 | Bremel Robert D | Retrovirus-based genomic screening |
| US7446190B2 (en) | 2002-05-28 | 2008-11-04 | Sloan-Kettering Institute For Cancer Research | Nucleic acids encoding chimeric T cell receptors |
| US20050129671A1 (en) | 2003-03-11 | 2005-06-16 | City Of Hope | Mammalian antigen-presenting T cells and bi-specific T cells |
| US7435596B2 (en) | 2004-11-04 | 2008-10-14 | St. Jude Children's Research Hospital, Inc. | Modified cell line and method for expansion of NK cell |
| DK2141997T3 (da) | 2007-03-30 | 2013-02-11 | Sloan Kettering Inst Cancer | Konstitutiv ekspression af co-stimulatoriske ligander på adoptivt overførte t-lymfocytter |
| DK2433713T3 (en) | 2007-12-07 | 2017-09-25 | Miltenyi Biotec Gmbh | CELL PROCESSING SYSTEMS AND PROCEDURES |
| US8479118B2 (en) | 2007-12-10 | 2013-07-02 | Microsoft Corporation | Switching search providers within a browser search box |
| US20120164718A1 (en) | 2008-05-06 | 2012-06-28 | Innovative Micro Technology | Removable/disposable apparatus for MEMS particle sorting device |
| JP5173594B2 (ja) | 2008-05-27 | 2013-04-03 | キヤノン株式会社 | 管理装置、画像形成装置及びそれらの処理方法 |
| ES2717629T3 (es) | 2009-11-03 | 2019-06-24 | Hope City | Receptor del factor de crecimiento epidérmico truncado (EGFRt) para selección de células T transducidas |
| PH12013501201A1 (en) | 2010-12-09 | 2013-07-29 | Univ Pennsylvania | Use of chimeric antigen receptor-modified t cells to treat cancer |
| RU2688185C2 (ru) | 2011-03-23 | 2019-05-21 | Фред Хатчинсон Кэнсер Рисерч Сентер | Способ и композиции для клеточной иммунотерапии |
| US8398282B2 (en) | 2011-05-12 | 2013-03-19 | Delphi Technologies, Inc. | Vehicle front lighting assembly and systems having a variable tint electrowetting element |
| JP6368243B2 (ja) | 2011-11-11 | 2018-08-08 | フレッド ハッチンソン キャンサー リサーチ センター | がんのためのサイクリンａ１に標的化されたｔ細胞免疫療法 |
| EP2814846B1 (en) | 2012-02-13 | 2020-01-08 | Seattle Children's Hospital d/b/a Seattle Children's Research Institute | Bispecific chimeric antigen receptors and therapeutic uses thereof |
| WO2013126726A1 (en) | 2012-02-22 | 2013-08-29 | The Trustees Of The University Of Pennsylvania | Double transgenic t cells comprising a car and a tcr and their methods of use |
| US9751928B2 (en) | 2012-05-03 | 2017-09-05 | Fred Hutchinson Cancer Research Center | Enhanced affinity T cell receptors and methods for making the same |
| KR102264290B1 (ko) | 2012-08-20 | 2021-06-10 | 프레드 헛친슨 켄서 리서치 센터 | 세포 면역요법을 위한 방법 및 조성물 |
| CN112458057A (zh) | 2012-10-02 | 2021-03-09 | 纪念斯隆-凯特琳癌症中心 | 用于免疫疗法的组合物和方法 |
| US9405601B2 (en) | 2012-12-20 | 2016-08-02 | Mitsubishi Electric Corporation | In-vehicle apparatus and program |
| EP2961831B1 (en) | 2013-02-26 | 2020-06-10 | Memorial Sloan Kettering Cancer Center | Compositions and methods for immunotherapy |
| EP3789487A1 (en) | 2013-04-03 | 2021-03-10 | Memorial Sloan Kettering Cancer Center | Effective generation of tumor-targeted t-cells derived from pluripotent stem cells |
| US9108442B2 (en) | 2013-08-20 | 2015-08-18 | Ricoh Company, Ltd. | Image forming apparatus |
| GB201318804D0 (en) | 2013-10-24 | 2013-12-11 | Adaptimmune Ltd | Vectors for transgene expression |
| SG11201605046YA (en) | 2013-12-20 | 2016-07-28 | Hutchinson Fred Cancer Res | Tagged chimeric effector molecules and receptors thereof |
| CN112877291A (zh) | 2014-04-10 | 2021-06-01 | 西雅图儿童医院 (Dba西雅图儿童研究所) | 基因修饰的t细胞产品、其制备方法和用途 |
| KR20160002030A (ko) * | 2014-06-30 | 2016-01-07 | 성지산업 주식회사 | 도플러 센서를 이용한 대상체 감지 방법 및 장치 |
| WO2016073602A2 (en) | 2014-11-05 | 2016-05-12 | Juno Therapeutics, Inc. | Methods for transduction and cell processing |
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| US20160199412A1 (en) | 2016-07-14 |
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| SA517381912B1 (ar) | 2021-12-30 |
| IL253399B2 (en) | 2023-06-01 |
| KR102554374B1 (ko) | 2023-07-11 |
| IL253399A0 (en) | 2017-09-28 |
| BR112017014917A2 (pt) | 2018-03-13 |
| MX391694B (es) | 2025-03-21 |
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| WO2016115177A1 (en) | 2016-07-21 |
| RU2017126975A (ru) | 2019-02-20 |
| CA2973483A1 (en) | 2016-07-21 |
| EP3245292B1 (en) | 2021-06-09 |
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