ES2865124T3 - Procedimiento en una etapa para la purificación de ácidos nuncleicos - Google Patents

Procedimiento en una etapa para la purificación de ácidos nuncleicos Download PDF

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Publication number
ES2865124T3
ES2865124T3 ES14765295T ES14765295T ES2865124T3 ES 2865124 T3 ES2865124 T3 ES 2865124T3 ES 14765295 T ES14765295 T ES 14765295T ES 14765295 T ES14765295 T ES 14765295T ES 2865124 T3 ES2865124 T3 ES 2865124T3
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nucleic acids
sample
solid substrate
gel
nucleic acid
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ES14765295T
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English (en)
Spanish (es)
Inventor
Gerard Gundling
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Abbott Molecular Inc
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Abbott Molecular Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Virology (AREA)
ES14765295T 2013-03-15 2014-03-14 Procedimiento en una etapa para la purificación de ácidos nuncleicos Active ES2865124T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361789622P 2013-03-15 2013-03-15
PCT/US2014/028518 WO2014144209A1 (en) 2013-03-15 2014-03-14 One-step procedure for the purification of nucleic acids

Publications (1)

Publication Number Publication Date
ES2865124T3 true ES2865124T3 (es) 2021-10-15

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ID=51528702

Family Applications (1)

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ES14765295T Active ES2865124T3 (es) 2013-03-15 2014-03-14 Procedimiento en una etapa para la purificación de ácidos nuncleicos

Country Status (6)

Country Link
US (2) US9803230B2 (cg-RX-API-DMAC7.html)
EP (2) EP3828284A1 (cg-RX-API-DMAC7.html)
JP (2) JP6431522B2 (cg-RX-API-DMAC7.html)
CN (1) CN105120986B (cg-RX-API-DMAC7.html)
ES (1) ES2865124T3 (cg-RX-API-DMAC7.html)
WO (1) WO2014144209A1 (cg-RX-API-DMAC7.html)

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US20200041449A1 (en) * 2016-10-04 2020-02-06 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
CN111278550B (zh) * 2017-06-30 2023-04-25 赛库乐米克斯公司 使用热塑性硅石纳米材料进行尺寸选择纯化
CN117500936A (zh) * 2021-05-06 2024-02-02 雅培分子公司 用于简单样品提取的组合物和方法
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WO2023034222A1 (en) * 2021-08-30 2023-03-09 Abbott Molecular Inc. Selective purification of rna
WO2024238645A2 (en) * 2023-05-16 2024-11-21 Atila Biosystems Incorporated Penetrable gel in a reaction vessel

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Also Published As

Publication number Publication date
CN105120986A (zh) 2015-12-02
US9803230B2 (en) 2017-10-31
US20180010168A1 (en) 2018-01-11
EP2969140A1 (en) 2016-01-20
JP2016514453A (ja) 2016-05-23
CN105120986B (zh) 2019-03-12
EP2969140B1 (en) 2021-02-17
US20140272944A1 (en) 2014-09-18
EP2969140A4 (en) 2017-05-03
WO2014144209A4 (en) 2014-11-20
EP3828284A1 (en) 2021-06-02
WO2014144209A1 (en) 2014-09-18
WO2014144209A8 (en) 2014-10-16
JP6431522B2 (ja) 2018-11-28
JP6684868B2 (ja) 2020-04-22
JP2018198599A (ja) 2018-12-20

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