ES2813437T3 - Métodos para mejorar la eficiencia de la transducción vectorial en linfocitos T - Google Patents

Métodos para mejorar la eficiencia de la transducción vectorial en linfocitos T Download PDF

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ES2813437T3
ES2813437T3 ES15818898T ES15818898T ES2813437T3 ES 2813437 T3 ES2813437 T3 ES 2813437T3 ES 15818898 T ES15818898 T ES 15818898T ES 15818898 T ES15818898 T ES 15818898T ES 2813437 T3 ES2813437 T3 ES 2813437T3
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primary
lymphocyte
transduction
lymphocytes
contacting
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Bitao Liang
Wei Liu
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Celgene Corp
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Celgene Corp
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
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    • C12N5/0636T lymphocytes
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    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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    • A61K2039/5158Antigen-pulsed cells, e.g. T-cells
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    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N2501/20Cytokines; Chemokines
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    • C12N2501/2302Interleukin-2 (IL-2)
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/51B7 molecules, e.g. CD80, CD86, CD28 (ligand), CD152 (ligand)
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50Cell markers; Cell surface determinants
    • C12N2501/515CD3, T-cell receptor complex
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2510/00Genetically modified cells
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    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/15011Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041Use of virus, viral particle or viral elements as a vector
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    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/15011Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041Use of virus, viral particle or viral elements as a vector
    • C12N2740/15042Use of virus, viral particle or viral elements as a vector virus or viral particle as vehicle, e.g. encapsulating small organic molecule

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  • Health & Medical Sciences (AREA)
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  • Hematology (AREA)
  • Physics & Mathematics (AREA)
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  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
ES15818898T 2014-07-11 2015-07-10 Métodos para mejorar la eficiencia de la transducción vectorial en linfocitos T Active ES2813437T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462023618P 2014-07-11 2014-07-11
PCT/US2015/039892 WO2016007827A1 (en) 2014-07-11 2015-07-10 Methods of improving vector transduction efficiency into t lymphocytes

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ES2813437T3 true ES2813437T3 (es) 2021-03-23

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ES15818898T Active ES2813437T3 (es) 2014-07-11 2015-07-10 Métodos para mejorar la eficiencia de la transducción vectorial en linfocitos T
ES20176483T Active ES2973524T3 (es) 2014-07-11 2015-07-10 Métodos para mejorar la eficacia de la transducción de vectores en los linfocitos T

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ES20176483T Active ES2973524T3 (es) 2014-07-11 2015-07-10 Métodos para mejorar la eficacia de la transducción de vectores en los linfocitos T

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US (2) US10982189B2 (enExample)
EP (2) EP3167058B1 (enExample)
JP (3) JP6754761B2 (enExample)
ES (2) ES2813437T3 (enExample)
WO (1) WO2016007827A1 (enExample)

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CN105247042B (zh) 2013-03-15 2021-06-11 普林斯顿大学理事会 用于高通量纯化的方法和设备
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
US10982189B2 (en) * 2014-07-11 2021-04-20 Celgene Corporation Methods of improving vector transduction efficiency into T lymphocytes
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
CA3074495A1 (en) 2017-09-01 2019-03-07 Gpb Scientific, Llc Methods for preparing therapeutically active cells using microfluidics
DE102018010282A1 (de) 2018-01-17 2019-07-18 Immatics US, Inc. Verfahren zur Feststellung der Wirksamkeit von viralen Vektoren
DE102018100967B4 (de) 2018-01-17 2019-08-14 Immatics US, Inc. Verfahren zur feststellung der wirksamkeit von viralen vektoren
CN108715865B (zh) * 2018-05-28 2021-09-21 福建师范大学 用于提高细胞转染效率的试剂组合物
EP3999081A1 (en) 2019-07-18 2022-05-25 GPB Scientific, Inc. Ordered processing of blood products to produce therapeutically active cells
KR20220129566A (ko) 2019-12-28 2022-09-23 지피비 싸이언티픽, 인크 입자 및 세포를 처리하기 위한 미세 유체 카트리지
KR20230167384A (ko) * 2021-04-06 2023-12-08 유니세트 바이오테크 씨오. 엘엘씨 γδ T 세포의 바이러스 형질 도입의 안정성을 개선하기 위한 방법 및 이의 응용
WO2023286088A1 (en) * 2021-07-16 2023-01-19 Indian Institute Of Science Education And Research Bhopal Methods and compositions for viral vector transduction
CN119530302B (zh) * 2025-01-22 2025-06-27 青岛华赛伯曼医学细胞生物有限公司 提高til细胞的慢病毒转导效率及转导稳定性的方法及试剂盒

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Also Published As

Publication number Publication date
ES2973524T3 (es) 2024-06-20
JP2017521101A (ja) 2017-08-03
JP2020191901A (ja) 2020-12-03
US11629335B2 (en) 2023-04-18
EP3167058A1 (en) 2017-05-17
US20210309966A1 (en) 2021-10-07
EP3750993A1 (en) 2020-12-16
US20170166866A1 (en) 2017-06-15
EP3167058B1 (en) 2020-05-27
JP6754761B2 (ja) 2020-09-16
EP3167058A4 (en) 2017-12-13
WO2016007827A1 (en) 2016-01-14
EP3750993B1 (en) 2023-12-27
JP2023040152A (ja) 2023-03-22
US10982189B2 (en) 2021-04-20

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