ES2729563T3 - Derivación sin alimentadores de células madre pluripotentes inducidas humanas con ARN mensajero sintético - Google Patents

Derivación sin alimentadores de células madre pluripotentes inducidas humanas con ARN mensajero sintético Download PDF

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Publication number
ES2729563T3
ES2729563T3 ES13790064T ES13790064T ES2729563T3 ES 2729563 T3 ES2729563 T3 ES 2729563T3 ES 13790064 T ES13790064 T ES 13790064T ES 13790064 T ES13790064 T ES 13790064T ES 2729563 T3 ES2729563 T3 ES 2729563T3
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cells
reprogramming
mrna
cell
somatic
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Spanish (es)
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Jiwu Wang
Luigi Warren
Yuhui Ni
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Allele Biotechnology and Pharmaceuticals Inc
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Allele Biotechnology and Pharmaceuticals Inc
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111General methods applicable to biologically active non-coding nucleic acids
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering nucleic acids [NA]
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/602Sox-2
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/606Transcription factors c-Myc
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/608Lin28
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
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    • C12N2510/00Genetically modified cells

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  • Transplantation (AREA)
  • Developmental Biology & Embryology (AREA)
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  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
ES13790064T 2012-05-13 2013-05-13 Derivación sin alimentadores de células madre pluripotentes inducidas humanas con ARN mensajero sintético Active ES2729563T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261646292P 2012-05-13 2012-05-13
PCT/US2013/040814 WO2013173248A2 (en) 2012-05-13 2013-05-13 Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

Publications (1)

Publication Number Publication Date
ES2729563T3 true ES2729563T3 (es) 2019-11-04

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ES19163001T Active ES2951822T3 (es) 2012-05-13 2013-05-13 Derivación sin alimentadores de células madre pluripotentes inducidas humanas con ARN mensajero sintético
ES13790064T Active ES2729563T3 (es) 2012-05-13 2013-05-13 Derivación sin alimentadores de células madre pluripotentes inducidas humanas con ARN mensajero sintético

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ES19163001T Active ES2951822T3 (es) 2012-05-13 2013-05-13 Derivación sin alimentadores de células madre pluripotentes inducidas humanas con ARN mensajero sintético

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EP (2) EP2850179B1 (enExample)
JP (2) JP6448531B2 (enExample)
KR (2) KR102215413B1 (enExample)
CN (2) CN104520424B (enExample)
AU (2) AU2013263101B2 (enExample)
CA (1) CA2872688C (enExample)
DK (1) DK2850179T3 (enExample)
ES (2) ES2951822T3 (enExample)
WO (1) WO2013173248A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014013664A2 (pt) 2011-12-05 2020-11-03 Factor Bioscience Inc. métodos e produtos para transfectar células
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
US10155929B2 (en) 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
US10119150B2 (en) 2012-05-13 2018-11-06 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
JP6448531B2 (ja) * 2012-05-13 2019-01-09 アリール バイオテクノロジー アンド ファーマシューティカルズ, インコーポレイテッド 合成メッセンジャーrnaを使用したヒト人工多能性幹細胞のフィーダーフリー誘導
BR122019025681B1 (pt) * 2012-11-01 2023-04-18 Factor Bioscience Inc Método para inserir uma sequência de ácido nucleico em uma localização segura de um genoma de uma célula
US9770489B2 (en) 2014-01-31 2017-09-26 Factor Bioscience Inc. Methods and products for nucleic acid production and delivery
US11241505B2 (en) 2015-02-13 2022-02-08 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
US11459560B2 (en) 2015-11-23 2022-10-04 The Regents Of The University Of Colorado Methods and compositions for reprogramming cells
US10576167B2 (en) 2016-08-17 2020-03-03 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
EP3541927A4 (en) * 2016-11-16 2020-11-25 Allele Biotechnology And Pharmaceuticals, Inc. INDUCTION OF PANCREATIC BETA CELLS BY DIFFERENTIATION OF STEM CELLS WITH RNA
US20180135023A1 (en) * 2016-11-16 2018-05-17 Allele Biotechnology And Pharmaceuticals, Inc. Induction of hepatocytes by stem cell differentiation with rna
KR102122336B1 (ko) * 2018-10-26 2020-06-12 서울시립대학교 산학협력단 세포전환용 rna 나노입자를 이용한 유도만능줄기세포의 제조방법
JP2022535192A (ja) 2019-05-14 2022-08-05 アレフ ファームス リミテッド 多能性細胞凝集体およびそれらの使用
KR20220039719A (ko) 2019-07-03 2022-03-29 팩터 바이오사이언스 인크. 양이온성 지질 및 이의 용도
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
US20230053060A1 (en) * 2020-01-24 2023-02-16 I Peace, Inc. Method for manufacturing induced pluripotent stem cells
JP2022104813A (ja) 2020-12-29 2022-07-11 アイ ピース,インコーポレイテッド リプログラミング因子を導入された細胞の培養方法

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US5260432A (en) * 1989-06-22 1993-11-09 Sloan-Kettering Institute For Cancer Research Human gamma retinoic acid receptor DNA
EP3231869A1 (en) * 2008-06-13 2017-10-18 Whitehead Institute for Biomedical Research Programming and reprogramming of cells
EP2192174B1 (en) * 2008-11-21 2015-11-11 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Reprogramming cells toward a pluripotent state
JP5898086B2 (ja) * 2009-11-04 2016-04-06 セルラー ダイナミクス インターナショナル, インコーポレイテッド 化学物質を用いるエピソームリプログラミング
LT3112467T (lt) * 2009-12-07 2018-06-25 The Trustees Of The University Of Pennsylvania Išgrynintą modifikuotą rnr apimantys rnr preparatai, skirti ląstelių perprogramavimui
CN102190731B (zh) * 2010-03-09 2016-01-27 中国科学院上海生命科学研究院 用人工转录因子诱导产生多能干细胞
US9580689B2 (en) * 2010-07-22 2017-02-28 Regents Of The University Of Minnesota Induced pluripotent stem cells
WO2012012798A2 (en) * 2010-07-23 2012-01-26 University Of South Florida Materials and methods for reduction of protein tau and treatment of neurodegenerative diseases
CN102417894B (zh) * 2011-10-21 2013-06-05 中国科学院广州生物医药与健康研究院 一种提高诱导生成多能性干细胞效率的方法
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
JP6448531B2 (ja) * 2012-05-13 2019-01-09 アリール バイオテクノロジー アンド ファーマシューティカルズ, インコーポレイテッド 合成メッセンジャーrnaを使用したヒト人工多能性幹細胞のフィーダーフリー誘導
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Also Published As

Publication number Publication date
KR102081811B1 (ko) 2020-02-26
EP2850179A4 (en) 2016-02-24
EP2850179B1 (en) 2019-03-20
WO2013173248A3 (en) 2014-12-18
EP3561054A1 (en) 2019-10-30
JP6448531B2 (ja) 2019-01-09
CN110241087B (zh) 2023-02-03
CN104520424B (zh) 2019-06-28
DK2850179T3 (da) 2019-06-17
ES2951822T3 (es) 2023-10-25
CA2872688A1 (en) 2013-11-21
JP2015517311A (ja) 2015-06-22
CN104520424A (zh) 2015-04-15
KR102215413B1 (ko) 2021-02-16
AU2019203335B2 (en) 2021-10-21
AU2019203335A1 (en) 2019-05-30
EP2850179A2 (en) 2015-03-25
KR20150035594A (ko) 2015-04-06
CN110241087A (zh) 2019-09-17
KR20200021553A (ko) 2020-02-28
CA2872688C (en) 2023-09-19
EP3561054C0 (en) 2023-07-05
AU2013263101A1 (en) 2014-11-27
EP3561054B1 (en) 2023-07-05
WO2013173248A2 (en) 2013-11-21
JP2017184766A (ja) 2017-10-12
JP6530452B2 (ja) 2019-06-12
AU2013263101B2 (en) 2019-02-14
HK1204005A1 (en) 2015-11-06

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