ES2729302T3 - Mutantes de recombinasas - Google Patents

Mutantes de recombinasas Download PDF

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Publication number
ES2729302T3
ES2729302T3 ES15779119T ES15779119T ES2729302T3 ES 2729302 T3 ES2729302 T3 ES 2729302T3 ES 15779119 T ES15779119 T ES 15779119T ES 15779119 T ES15779119 T ES 15779119T ES 2729302 T3 ES2729302 T3 ES 2729302T3
Authority
ES
Spain
Prior art keywords
uvsx
nucleic acid
seq
recombinase
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES15779119T
Other languages
English (en)
Spanish (es)
Inventor
Erin Bomati
Matthew William Kellinger
Jonathan Mark Boutell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Cambridge Ltd
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Application granted granted Critical
Publication of ES2729302T3 publication Critical patent/ES2729302T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01008—ATP diphosphatase (3.6.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES15779119T 2014-09-29 2015-09-29 Mutantes de recombinasas Active ES2729302T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462057056P 2014-09-29 2014-09-29
PCT/US2015/053012 WO2016054088A1 (en) 2014-09-29 2015-09-29 Recombinase mutants

Publications (1)

Publication Number Publication Date
ES2729302T3 true ES2729302T3 (es) 2019-10-31

Family

ID=54293382

Family Applications (1)

Application Number Title Priority Date Filing Date
ES15779119T Active ES2729302T3 (es) 2014-09-29 2015-09-29 Mutantes de recombinasas

Country Status (18)

Country Link
US (2) US9982244B2 (enExample)
EP (1) EP3201222B1 (enExample)
JP (2) JP2017529093A (enExample)
KR (3) KR20170065028A (enExample)
CN (1) CN107208075B (enExample)
AU (1) AU2015323936B2 (enExample)
BR (1) BR112017006542B1 (enExample)
CA (1) CA2962941C (enExample)
DK (1) DK3201222T3 (enExample)
ES (1) ES2729302T3 (enExample)
IL (1) IL251422B (enExample)
MX (1) MX385063B (enExample)
MY (1) MY182395A (enExample)
NZ (1) NZ730593A (enExample)
RU (1) RU2721920C2 (enExample)
SG (1) SG11201702493WA (enExample)
WO (1) WO2016054088A1 (enExample)
ZA (1) ZA201702248B (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017529093A (ja) * 2014-09-29 2017-10-05 イルミナ ケンブリッジ リミテッド リコンビナーゼ変異体
WO2017172699A1 (en) * 2016-03-28 2017-10-05 Illumina, Inc. Recombinase mutants
JP7048609B2 (ja) * 2016-12-09 2022-04-05 ボリアル ジェノミクス, インコーポレイテッド 連結型ライゲーション
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN108913700B (zh) * 2018-08-16 2021-04-09 深圳市艾伟迪生物科技有限公司 gp32单链结合蛋白的制备方法、表达基因、重组表达载体及应用
CN115975956A (zh) * 2022-02-23 2023-04-18 杨挥 一种重组酶突变体及包含该突变体的核酸等温扩增反应体系

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
US7742463B2 (en) 2005-08-18 2010-06-22 Hong Kong Applied Science And Technology Research Institute Co., Ltd. Security gatekeeper for a packetized voice communication network
EP2029782B1 (en) * 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
WO2010141940A1 (en) * 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
CN103153960B (zh) 2010-09-21 2015-02-25 巴斯夫欧洲公司 制备取代的n-苯基羟胺的方法
EP3978620A1 (en) * 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
JP2017529093A (ja) * 2014-09-29 2017-10-05 イルミナ ケンブリッジ リミテッド リコンビナーゼ変異体

Also Published As

Publication number Publication date
AU2015323936A1 (en) 2017-04-20
SG11201702493WA (en) 2017-04-27
KR102126641B1 (ko) 2020-06-24
CA2962941C (en) 2022-06-14
KR20200026320A (ko) 2020-03-10
JP2019213545A (ja) 2019-12-19
IL251422A0 (en) 2017-05-29
BR112017006542B1 (pt) 2023-11-14
JP6879519B2 (ja) 2021-06-02
US10344269B2 (en) 2019-07-09
DK3201222T3 (da) 2019-06-24
JP2017529093A (ja) 2017-10-05
NZ730593A (en) 2023-06-30
WO2016054088A1 (en) 2016-04-07
US20160090581A1 (en) 2016-03-31
CN107208075B (zh) 2021-08-24
US9982244B2 (en) 2018-05-29
IL251422B (en) 2020-10-29
EP3201222A1 (en) 2017-08-09
MY182395A (en) 2021-01-23
RU2017110582A3 (enExample) 2019-04-15
CN107208075A (zh) 2017-09-26
RU2721920C2 (ru) 2020-05-25
AU2015323936B2 (en) 2021-03-11
ZA201702248B (en) 2019-06-26
US20180258409A1 (en) 2018-09-13
MX2017004034A (es) 2017-11-17
KR20170065028A (ko) 2017-06-12
BR112017006542A2 (pt) 2018-01-23
CA2962941A1 (en) 2016-04-07
MX385063B (es) 2025-03-14
EP3201222B1 (en) 2019-04-10
KR20190037364A (ko) 2019-04-05
RU2017110582A (ru) 2018-11-07

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