CN107208075B - 重组酶突变体 - Google Patents

重组酶突变体 Download PDF

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Publication number
CN107208075B
CN107208075B CN201580059870.9A CN201580059870A CN107208075B CN 107208075 B CN107208075 B CN 107208075B CN 201580059870 A CN201580059870 A CN 201580059870A CN 107208075 B CN107208075 B CN 107208075B
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Chinese (zh)
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CN107208075A (zh
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E.博马蒂
M.W.凯林格
J.M.鲍特尔
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Illumina Cambridge Ltd
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Illumina Cambridge Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01008—ATP diphosphatase (3.6.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201580059870.9A 2014-09-29 2015-09-29 重组酶突变体 Active CN107208075B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462057056P 2014-09-29 2014-09-29
US62/057,056 2014-09-29
PCT/US2015/053012 WO2016054088A1 (en) 2014-09-29 2015-09-29 Recombinase mutants

Publications (2)

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CN107208075A CN107208075A (zh) 2017-09-26
CN107208075B true CN107208075B (zh) 2021-08-24

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CN201580059870.9A Active CN107208075B (zh) 2014-09-29 2015-09-29 重组酶突变体

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US (2) US9982244B2 (enExample)
EP (1) EP3201222B1 (enExample)
JP (2) JP2017529093A (enExample)
KR (3) KR20170065028A (enExample)
CN (1) CN107208075B (enExample)
AU (1) AU2015323936B2 (enExample)
BR (1) BR112017006542B1 (enExample)
CA (1) CA2962941C (enExample)
DK (1) DK3201222T3 (enExample)
ES (1) ES2729302T3 (enExample)
IL (1) IL251422B (enExample)
MX (1) MX385063B (enExample)
MY (1) MY182395A (enExample)
NZ (1) NZ730593A (enExample)
RU (1) RU2721920C2 (enExample)
SG (1) SG11201702493WA (enExample)
WO (1) WO2016054088A1 (enExample)
ZA (1) ZA201702248B (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017529093A (ja) * 2014-09-29 2017-10-05 イルミナ ケンブリッジ リミテッド リコンビナーゼ変異体
WO2017172699A1 (en) * 2016-03-28 2017-10-05 Illumina, Inc. Recombinase mutants
JP7048609B2 (ja) * 2016-12-09 2022-04-05 ボリアル ジェノミクス, インコーポレイテッド 連結型ライゲーション
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN108913700B (zh) * 2018-08-16 2021-04-09 深圳市艾伟迪生物科技有限公司 gp32单链结合蛋白的制备方法、表达基因、重组表达载体及应用
CN115975956A (zh) * 2022-02-23 2023-04-18 杨挥 一种重组酶突变体及包含该突变体的核酸等温扩增反应体系

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008035205A2 (en) * 2006-05-04 2008-03-27 Asm Scientific, Inc. Recombinase polymerase amplification
WO2010141940A1 (en) * 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2013188582A1 (en) * 2012-06-15 2013-12-19 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries

Family Cites Families (10)

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Publication number Priority date Publication date Assignee Title
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
US7742463B2 (en) 2005-08-18 2010-06-22 Hong Kong Applied Science And Technology Research Institute Co., Ltd. Security gatekeeper for a packetized voice communication network
CN103153960B (zh) 2010-09-21 2015-02-25 巴斯夫欧洲公司 制备取代的n-苯基羟胺的方法
EP3978620A1 (en) * 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
JP2017529093A (ja) * 2014-09-29 2017-10-05 イルミナ ケンブリッジ リミテッド リコンビナーゼ変異体

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008035205A2 (en) * 2006-05-04 2008-03-27 Asm Scientific, Inc. Recombinase polymerase amplification
WO2010141940A1 (en) * 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2013188582A1 (en) * 2012-06-15 2013-12-19 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries

Also Published As

Publication number Publication date
AU2015323936A1 (en) 2017-04-20
SG11201702493WA (en) 2017-04-27
KR102126641B1 (ko) 2020-06-24
CA2962941C (en) 2022-06-14
KR20200026320A (ko) 2020-03-10
JP2019213545A (ja) 2019-12-19
IL251422A0 (en) 2017-05-29
BR112017006542B1 (pt) 2023-11-14
JP6879519B2 (ja) 2021-06-02
US10344269B2 (en) 2019-07-09
DK3201222T3 (da) 2019-06-24
JP2017529093A (ja) 2017-10-05
NZ730593A (en) 2023-06-30
WO2016054088A1 (en) 2016-04-07
US20160090581A1 (en) 2016-03-31
US9982244B2 (en) 2018-05-29
IL251422B (en) 2020-10-29
EP3201222A1 (en) 2017-08-09
MY182395A (en) 2021-01-23
RU2017110582A3 (enExample) 2019-04-15
CN107208075A (zh) 2017-09-26
RU2721920C2 (ru) 2020-05-25
AU2015323936B2 (en) 2021-03-11
ZA201702248B (en) 2019-06-26
US20180258409A1 (en) 2018-09-13
MX2017004034A (es) 2017-11-17
KR20170065028A (ko) 2017-06-12
ES2729302T3 (es) 2019-10-31
BR112017006542A2 (pt) 2018-01-23
CA2962941A1 (en) 2016-04-07
MX385063B (es) 2025-03-14
EP3201222B1 (en) 2019-04-10
KR20190037364A (ko) 2019-04-05
RU2017110582A (ru) 2018-11-07

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