ES2656961T3 - Uso de G-Clamp para mejorar la PCR específica de alelo - Google Patents
Uso de G-Clamp para mejorar la PCR específica de alelo Download PDFInfo
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- ES2656961T3 ES2656961T3 ES12770415.3T ES12770415T ES2656961T3 ES 2656961 T3 ES2656961 T3 ES 2656961T3 ES 12770415 T ES12770415 T ES 12770415T ES 2656961 T3 ES2656961 T3 ES 2656961T3
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- oligonucleotide
- nucleotide
- variant
- target sequence
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
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- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161538650P | 2011-09-23 | 2011-09-23 | |
| US201161538650P | 2011-09-23 | ||
| PCT/EP2012/003817 WO2013041194A1 (en) | 2011-09-23 | 2012-09-12 | Use of g-clamp for improved allele-specific pcr |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2656961T3 true ES2656961T3 (es) | 2018-03-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12770415.3T Active ES2656961T3 (es) | 2011-09-23 | 2012-09-12 | Uso de G-Clamp para mejorar la PCR específica de alelo |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9476091B2 (enExample) |
| EP (1) | EP2758546B1 (enExample) |
| JP (1) | JP6050820B2 (enExample) |
| CN (2) | CN107267615A (enExample) |
| CA (1) | CA2847943C (enExample) |
| ES (1) | ES2656961T3 (enExample) |
| WO (1) | WO2013041194A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0684425B2 (ja) | 1988-02-17 | 1994-10-26 | 帝人株式会社 | ポリエステルの製造法 |
| EP2758546B1 (en) | 2011-09-23 | 2017-11-22 | Roche Diagnostics GmbH | Use of g-clamp for improved allele-specific pcr |
| US9382581B2 (en) | 2012-12-13 | 2016-07-05 | Roche Molecular Systems, Inc. | Primers with modified phosphate and base in allele-specific PCR |
| CN108130373A (zh) * | 2013-03-13 | 2018-06-08 | 豪夫迈·罗氏有限公司 | 在人pi3kca (pik3ca)基因中检测突变的方法和组合物 |
| CN109477097A (zh) | 2016-04-20 | 2019-03-15 | Jbs科学公司 | 检测CTNNBl和HTERT突变的试剂盒和方法及其在HCC检测和疾病管理中的用途 |
| CN112359112A (zh) * | 2020-11-11 | 2021-02-12 | 凯杰生物工程（深圳）有限公司 | 一种用于检测calr基因突变的试剂和试剂盒 |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
| US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DE3529478A1 (de) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
| US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5216141A (en) | 1988-06-06 | 1993-06-01 | Benner Steven A | Oligonucleotide analogs containing sulfur linkages |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| WO1991012342A1 (en) | 1990-02-16 | 1991-08-22 | F. Hoffmann-La Roche Ag | Improvements in the specificity and convenience of the polymerase chain reaction |
| US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
| US5386023A (en) | 1990-07-27 | 1995-01-31 | Isis Pharmaceuticals | Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| EP0553264A4 (en) | 1990-10-05 | 1994-07-13 | Wayne M Barnes | Thermostable dna polymerase |
| JPH04234422A (ja) | 1990-10-31 | 1992-08-24 | Internatl Business Mach Corp <Ibm> | 二重硬化エポキシバックシール処方物 |
| US5840867A (en) | 1991-02-21 | 1998-11-24 | Gilead Sciences, Inc. | Aptamer analogs specific for biomolecules |
| US5644048A (en) | 1992-01-10 | 1997-07-01 | Isis Pharmaceuticals, Inc. | Process for preparing phosphorothioate oligonucleotides |
| DE4234086A1 (de) | 1992-02-05 | 1993-08-12 | Diagen Inst Molekularbio | Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5637684A (en) | 1994-02-23 | 1997-06-10 | Isis Pharmaceuticals, Inc. | Phosphoramidate and phosphorothioamidate oligomeric compounds |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| DE69739357D1 (de) | 1996-06-04 | 2009-05-28 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| US6361940B1 (en) * | 1996-09-24 | 2002-03-26 | Qiagen Genomics, Inc. | Compositions and methods for enhancing hybridization and priming specificity |
| US6028183A (en) | 1997-11-07 | 2000-02-22 | Gilead Sciences, Inc. | Pyrimidine derivatives and oligonucleotides containing same |
| US20030027135A1 (en) * | 2001-03-02 | 2003-02-06 | Ecker David J. | Method for rapid detection and identification of bioagents |
| US20030228571A1 (en) * | 2002-04-01 | 2003-12-11 | Ecker David J. | Method for rapid detection and identification of viral bioagents |
| CN1197976C (zh) * | 2003-01-31 | 2005-04-20 | 中国人民解放军南京军区联勤部军事医学研究所 | 基因多态性检测方法 |
| US7408051B2 (en) | 2004-04-14 | 2008-08-05 | Applera Corporation | Modified oligonucleotides and applications thereof |
| CN1737162A (zh) * | 2005-03-16 | 2006-02-22 | 南京中医药大学附属医院(江苏省中医院) | 表皮生长因子受体(egfr)基因测序检测方法 |
| US8999634B2 (en) * | 2007-04-27 | 2015-04-07 | Quest Diagnostics Investments Incorporated | Nucleic acid detection combining amplification with fragmentation |
| EP2337865B1 (en) * | 2008-10-20 | 2014-11-19 | Roche Diagnostics GmbH | Allele-specific amplification using a primer with a modified nucleotide |
| US20120164641A1 (en) * | 2010-12-22 | 2012-06-28 | Roche Molecular Systems, Inc. | Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene |
| EP2758546B1 (en) | 2011-09-23 | 2017-11-22 | Roche Diagnostics GmbH | Use of g-clamp for improved allele-specific pcr |
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2012
- 2012-09-12 EP EP12770415.3A patent/EP2758546B1/en active Active
- 2012-09-12 JP JP2014531125A patent/JP6050820B2/ja active Active
- 2012-09-12 WO PCT/EP2012/003817 patent/WO2013041194A1/en not_active Ceased
- 2012-09-12 CA CA2847943A patent/CA2847943C/en active Active
- 2012-09-12 CN CN201710514760.8A patent/CN107267615A/zh active Pending
- 2012-09-12 ES ES12770415.3T patent/ES2656961T3/es active Active
- 2012-09-12 CN CN201280045391.8A patent/CN103797132A/zh active Pending
- 2012-09-21 US US13/624,077 patent/US9476091B2/en active Active
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Also Published As
| Publication number | Publication date |
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| WO2013041194A1 (en) | 2013-03-28 |
| US20130078630A1 (en) | 2013-03-28 |
| CN107267615A (zh) | 2017-10-20 |
| CA2847943A1 (en) | 2013-03-28 |
| US20160369330A1 (en) | 2016-12-22 |
| EP2758546B1 (en) | 2017-11-22 |
| US9476091B2 (en) | 2016-10-25 |
| CN103797132A (zh) | 2014-05-14 |
| CA2847943C (en) | 2017-08-29 |
| JP6050820B2 (ja) | 2016-12-21 |
| EP2758546A1 (en) | 2014-07-30 |
| JP2014527823A (ja) | 2014-10-23 |
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