ES2648045T3 - Producción mejorada de riboflavina - Google Patents
Producción mejorada de riboflavina Download PDFInfo
- Publication number
- ES2648045T3 ES2648045T3 ES09751895.5T ES09751895T ES2648045T3 ES 2648045 T3 ES2648045 T3 ES 2648045T3 ES 09751895 T ES09751895 T ES 09751895T ES 2648045 T3 ES2648045 T3 ES 2648045T3
- Authority
- ES
- Spain
- Prior art keywords
- rib
- riboflavin
- polynucleotide
- seq
- leader sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 title abstract description 56
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- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 229940103141 riboflavin 100 mg Drugs 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229940073490 sodium glutamate Drugs 0.000 description 1
- GFWRVVCDTLRWPK-KPKJPENVSA-N sofalcone Chemical compound C1=CC(OCC=C(C)C)=CC=C1\C=C\C(=O)C1=CC=C(OCC=C(C)C)C=C1OCC(O)=O GFWRVVCDTLRWPK-KPKJPENVSA-N 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 101150079396 trpC2 gene Proteins 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08019492A EP2186880A1 (en) | 2008-11-07 | 2008-11-07 | Improved production of riboflavin |
| EP08019492 | 2008-11-07 | ||
| PCT/EP2009/064825 WO2010052319A1 (en) | 2008-11-07 | 2009-11-09 | Improved production of riboflavin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2648045T3 true ES2648045T3 (es) | 2017-12-28 |
Family
ID=40329198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09751895.5T Active ES2648045T3 (es) | 2008-11-07 | 2009-11-09 | Producción mejorada de riboflavina |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10633684B2 (enExample) |
| EP (2) | EP2186880A1 (enExample) |
| JP (1) | JP5787089B2 (enExample) |
| KR (1) | KR101726886B1 (enExample) |
| CN (2) | CN103757000A (enExample) |
| ES (1) | ES2648045T3 (enExample) |
| WO (1) | WO2010052319A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101335853B1 (ko) * | 2011-12-01 | 2013-12-02 | 씨제이제일제당 (주) | Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법 |
| DK3102669T3 (da) * | 2014-02-07 | 2019-08-19 | Dsm Ip Assets Bv | Forbedret bacillevært |
| CN103865944B (zh) * | 2014-02-20 | 2016-01-20 | 天津大学 | 一种生产核黄素的大肠杆菌菌株及构建方法及用途 |
| KR102561864B1 (ko) * | 2014-11-19 | 2023-08-01 | 바스프 에스이 | Rib７ 프로모터를 사용하여 유전자 발현을 하향조절하기 위한 에레모테시움의 유전적 변형 |
| WO2017036903A1 (en) * | 2015-08-28 | 2017-03-09 | Dsm Ip Assets B.V. | Improved vitamin production |
| KR101844778B1 (ko) * | 2017-01-18 | 2018-04-04 | 주식회사한국야쿠르트 | 비타민 b2 생합성능이 매우 우수한 락토바실러스 플란타룸 hy7715 및 이를 유효성분으로 함유하는 제품 |
| CN108330136A (zh) * | 2017-05-05 | 2018-07-27 | 北京航空航天大学 | 一种构建产黄素单核苷酸的工程菌株的方法及其应用 |
| US20210032639A1 (en) * | 2017-09-13 | 2021-02-04 | Danisco Us Inc. | Modified 5'-untranslated region (utr) sequences for increased protein production in bacillus |
| CN111935981B (zh) | 2018-04-05 | 2024-06-14 | 帝斯曼知识产权资产管理有限公司 | 变体麦芽糖α-淀粉酶 |
| EA202191360A1 (ru) * | 2018-11-15 | 2021-08-11 | ДСМ АйПи АССЕТС Б.В. | Усовершенствованное получение рибофлавина |
| CN115715328A (zh) | 2020-06-23 | 2023-02-24 | 帝斯曼知识产权资产管理有限公司 | 发酵方法 |
| CN114592000B (zh) * | 2020-12-03 | 2023-07-21 | 上海市农业科学院 | 一种六基因组合在水稻种子中提高vb2含量的应用及方法 |
| CN112538453B (zh) * | 2020-12-15 | 2022-07-29 | 通辽梅花生物科技有限公司 | 一种产核黄素的枯草芽孢杆菌及其构建方法与应用 |
| CN116463305B (zh) * | 2023-06-15 | 2023-10-17 | 北京易醒生物科技有限公司 | 一种提高用于乙醇氧化的醇氧化酶表达量的方法、优化的核黄素生物合成基因 |
| CN117660577B (zh) * | 2023-12-08 | 2024-06-25 | 苏州华赛生物工程技术有限公司 | LtaSA蛋白或其编码基因在核黄素生产中的应用 |
| CN117568433B (zh) * | 2024-01-15 | 2024-04-16 | 黑龙江新和成生物科技有限公司 | 一种提高枯草芽孢杆菌发酵生产核黄素的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5837528A (en) * | 1989-06-22 | 1998-11-17 | Hoffmann La Roche, Inc. | Bacterial strains which overproduce riboflavin |
| CN1066486C (zh) | 1989-06-22 | 2001-05-30 | 霍夫曼-拉罗奇有限公司 | 高产核黄素的细菌菌株 |
| US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
| US6656721B1 (en) | 2000-08-08 | 2003-12-02 | Roche Vitamins, Inc. | Polynucleotide portions of the biotin operon from B. subtilis for use in enhanced fermentation |
| DE602004029897D1 (de) | 2003-06-02 | 2010-12-16 | Dsm Ip Assets Bv | Herstellung von thiamin durch fermentation |
| WO2004113510A2 (en) | 2003-06-18 | 2004-12-29 | Dsm Ip Assets B.V. | Production of pantothenate using microorganisms incapable of sporulation |
| CN101300344B (zh) | 2005-11-02 | 2012-07-04 | 帝斯曼知识产权资产管理有限公司 | 经修饰的转酮酶及其用途 |
-
2008
- 2008-11-07 EP EP08019492A patent/EP2186880A1/en not_active Withdrawn
-
2009
- 2009-11-09 JP JP2011533772A patent/JP5787089B2/ja active Active
- 2009-11-09 CN CN201310713983.9A patent/CN103757000A/zh active Pending
- 2009-11-09 US US13/127,618 patent/US10633684B2/en active Active
- 2009-11-09 ES ES09751895.5T patent/ES2648045T3/es active Active
- 2009-11-09 WO PCT/EP2009/064825 patent/WO2010052319A1/en not_active Ceased
- 2009-11-09 KR KR1020117012802A patent/KR101726886B1/ko active Active
- 2009-11-09 EP EP09751895.5A patent/EP2356211B1/en active Active
- 2009-11-09 CN CN200980144726.XA patent/CN102209780B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR20110093847A (ko) | 2011-08-18 |
| EP2186880A1 (en) | 2010-05-19 |
| EP2356211B1 (en) | 2017-09-20 |
| US10633684B2 (en) | 2020-04-28 |
| JP5787089B2 (ja) | 2015-09-30 |
| CN102209780B (zh) | 2016-02-10 |
| WO2010052319A1 (en) | 2010-05-14 |
| EP2356211A1 (en) | 2011-08-17 |
| CN103757000A (zh) | 2014-04-30 |
| CN102209780A (zh) | 2011-10-05 |
| US20110312025A1 (en) | 2011-12-22 |
| JP2012507984A (ja) | 2012-04-05 |
| KR101726886B1 (ko) | 2017-04-13 |
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