JP5787089B2 - リボフラビンの改善された生産 - Google Patents

リボフラビンの改善された生産 Download PDF

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Publication number
JP5787089B2
JP5787089B2 JP2011533772A JP2011533772A JP5787089B2 JP 5787089 B2 JP5787089 B2 JP 5787089B2 JP 2011533772 A JP2011533772 A JP 2011533772A JP 2011533772 A JP2011533772 A JP 2011533772A JP 5787089 B2 JP5787089 B2 JP 5787089B2
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Japan
Prior art keywords
riboflavin
rib
polynucleotide
leader
seq
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JP2011533772A
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English (en)
Japanese (ja)
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JP2012507984A (ja
JP2012507984A5 (enExample
Inventor
マーティン レーマン，
マーティン レーマン，
ミハエル ハンス，
ミハエル ハンス，
ハンスーピーター ホーマン，
ハンスーピーター ホーマン，
ディエトマール ロデルト，
ディエトマール ロデルト，
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DSM IP Assets BV
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DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2011533772A 2008-11-07 2009-11-09 リボフラビンの改善された生産 Active JP5787089B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08019492A EP2186880A1 (en) 2008-11-07 2008-11-07 Improved production of riboflavin
EP08019492.1 2008-11-07
PCT/EP2009/064825 WO2010052319A1 (en) 2008-11-07 2009-11-09 Improved production of riboflavin

Publications (3)

Publication Number Publication Date
JP2012507984A JP2012507984A (ja) 2012-04-05
JP2012507984A5 JP2012507984A5 (enExample) 2012-12-27
JP5787089B2 true JP5787089B2 (ja) 2015-09-30

Family

ID=40329198

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011533772A Active JP5787089B2 (ja) 2008-11-07 2009-11-09 リボフラビンの改善された生産

Country Status (7)

Country Link
US (1) US10633684B2 (enExample)
EP (2) EP2186880A1 (enExample)
JP (1) JP5787089B2 (enExample)
KR (1) KR101726886B1 (enExample)
CN (2) CN102209780B (enExample)
ES (1) ES2648045T3 (enExample)
WO (1) WO2010052319A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101335853B1 (ko) 2011-12-01 2013-12-02 씨제이제일제당 (주) Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법
DK3102669T3 (da) * 2014-02-07 2019-08-19 Dsm Ip Assets Bv Forbedret bacillevært
CN103865944B (zh) * 2014-02-20 2016-01-20 天津大学 一种生产核黄素的大肠杆菌菌株及构建方法及用途
KR102561864B1 (ko) * 2014-11-19 2023-08-01 바스프 에스이 Rib７ 프로모터를 사용하여 유전자 발현을 하향조절하기 위한 에레모테시움의 유전적 변형
CN107922464B (zh) * 2015-08-28 2022-03-15 帝斯曼知识产权资产管理有限公司 经改进的维生素生产
KR101844778B1 (ko) * 2017-01-18 2018-04-04 주식회사한국야쿠르트 비타민 b2 생합성능이 매우 우수한 락토바실러스 플란타룸 hy7715 및 이를 유효성분으로 함유하는 제품
CN108330136A (zh) * 2017-05-05 2018-07-27 北京航空航天大学 一种构建产黄素单核苷酸的工程菌株的方法及其应用
KR20200047668A (ko) * 2017-09-13 2020-05-07 다니스코 유에스 인크. 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열
CN111935981B (zh) 2018-04-05 2024-06-14 帝斯曼知识产权资产管理有限公司 变体麦芽糖α-淀粉酶
CN113015811A (zh) * 2018-11-15 2021-06-22 帝斯曼知识产权资产管理有限公司 改善的核黄素生产
WO2021260057A2 (en) 2020-06-23 2021-12-30 Dsm Ip Assets B.V. Fermentation process
CN114592000B (zh) * 2020-12-03 2023-07-21 上海市农业科学院 一种六基因组合在水稻种子中提高vb2含量的应用及方法
CN112538453B (zh) * 2020-12-15 2022-07-29 通辽梅花生物科技有限公司 一种产核黄素的枯草芽孢杆菌及其构建方法与应用
CN116463305B (zh) * 2023-06-15 2023-10-17 北京易醒生物科技有限公司 一种提高用于乙醇氧化的醇氧化酶表达量的方法、优化的核黄素生物合成基因
CN117660577B (zh) * 2023-12-08 2024-06-25 苏州华赛生物工程技术有限公司 LtaSA蛋白或其编码基因在核黄素生产中的应用
CN117568433B (zh) * 2024-01-15 2024-04-16 黑龙江新和成生物科技有限公司 一种提高枯草芽孢杆菌发酵生产核黄素的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1066486C (zh) * 1989-06-22 2001-05-30 霍夫曼-拉罗奇有限公司 高产核黄素的细菌菌株
US5837528A (en) * 1989-06-22 1998-11-17 Hoffmann La Roche, Inc. Bacterial strains which overproduce riboflavin
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US6656721B1 (en) 2000-08-08 2003-12-02 Roche Vitamins, Inc. Polynucleotide portions of the biotin operon from B. subtilis for use in enhanced fermentation
US20060127993A1 (en) 2003-06-02 2006-06-15 Goese Markus G Thiamin production by fermentation
KR101185656B1 (ko) 2003-06-18 2012-09-24 디에스엠 아이피 어셋츠 비.브이. 포자를 형성할 수 없는 미생물을 사용한 판토텐산염의 제조방법
JP5364376B2 (ja) 2005-11-02 2013-12-11 ディーエスエム アイピー アセッツ ビー．ブイ． 改変型トランスケトラーゼおよびその使用

Also Published As

Publication number Publication date
EP2356211A1 (en) 2011-08-17
JP2012507984A (ja) 2012-04-05
CN102209780B (zh) 2016-02-10
CN103757000A (zh) 2014-04-30
US10633684B2 (en) 2020-04-28
WO2010052319A1 (en) 2010-05-14
US20110312025A1 (en) 2011-12-22
CN102209780A (zh) 2011-10-05
EP2356211B1 (en) 2017-09-20
KR20110093847A (ko) 2011-08-18
EP2186880A1 (en) 2010-05-19
KR101726886B1 (ko) 2017-04-13
ES2648045T3 (es) 2017-12-28

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