ES2609848T3 - Nuevos genes marcadores seleccionables - Google Patents
Nuevos genes marcadores seleccionables Download PDFInfo
- Publication number
- ES2609848T3 ES2609848T3 ES13160763.2T ES13160763T ES2609848T3 ES 2609848 T3 ES2609848 T3 ES 2609848T3 ES 13160763 T ES13160763 T ES 13160763T ES 2609848 T3 ES2609848 T3 ES 2609848T3
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- Prior art keywords
- cells
- vector
- selectable marker
- marker genes
- polynucleotide
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8277—Phosphinotricin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01183—Phosphinothricin acetyltransferase (2.3.1.183)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Un método de selección de una célula vegetal, en el que dicho método comprende: proporcionar un vector a una pluralidad de células vegetales, comprendiendo el vector: un promotor operable en una célula vegetal y un polinucleótido unido operablemente a dicho promotor, donde dicho polinucleótido codifica una proteína que tiene actividad de fosfinotricina acetiltransferasa y en donde dicha proteína tiene al menos 90% de identidad de secuencia con la secuencia de aminoácidos de SEQ ID NO: 2; y cultivar dicha pluralidad de células en una concentración de un herbicida que permite que las células que expresan dicho polinucleótido crezcan mientras se matan o inhibe el crecimiento de células que no comprenden dicho vector, en el que dicho herbicida comprende fosfinotricina.
Description
Claims (1)
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imagen1 imagen2 imagen3
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US87360206P | 2006-12-07 | 2006-12-07 | |
US873602P | 2006-12-07 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2609848T3 true ES2609848T3 (es) | 2017-04-24 |
Family
ID=39493101
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES07869044.3T Active ES2553331T3 (es) | 2006-12-07 | 2007-12-07 | Nuevos genes marcadores seleccionables |
ES13160763.2T Active ES2609848T3 (es) | 2006-12-07 | 2007-12-07 | Nuevos genes marcadores seleccionables |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES07869044.3T Active ES2553331T3 (es) | 2006-12-07 | 2007-12-07 | Nuevos genes marcadores seleccionables |
Country Status (17)
Country | Link |
---|---|
US (3) | US10752913B2 (es) |
EP (2) | EP2094831B1 (es) |
JP (1) | JP5563825B2 (es) |
CN (3) | CN106987561A (es) |
AR (2) | AR064200A1 (es) |
AU (1) | AU2007329208B2 (es) |
BR (1) | BRPI0720226B8 (es) |
CA (1) | CA2671365C (es) |
DK (2) | DK2610334T3 (es) |
ES (2) | ES2553331T3 (es) |
HU (2) | HUE031454T2 (es) |
MX (1) | MX2009006093A (es) |
NZ (1) | NZ577363A (es) |
PL (1) | PL2610334T3 (es) |
PT (1) | PT2610334T (es) |
SI (1) | SI2610334T1 (es) |
WO (1) | WO2008070845A2 (es) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
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US8563805B2 (en) | 2008-02-27 | 2013-10-22 | Okanagan Specialty Fruits Inc. | Genetically modified reduced-browning fruit-producing plant and produced fruit thereof, and method of obtaining such |
CN102118966A (zh) * | 2008-06-11 | 2011-07-06 | 陶氏益农公司 | 表达除草剂耐受基因的构建体、相关植物和相关的性状组合 |
WO2010046422A2 (en) * | 2008-10-22 | 2010-04-29 | Basf Se | Use of auxin type herbicides on cultivated plants |
WO2010046420A2 (en) * | 2008-10-22 | 2010-04-29 | Basf Se | Use of protoporphyrinogen oxidase inhibitors on cultivated plants |
EP2362723A1 (en) | 2008-11-04 | 2011-09-07 | Dow Agrosciences LLC | Omega-9 quality brassica juncea |
KR101787776B1 (ko) | 2009-08-19 | 2017-10-18 | 다우 아그로사이언시즈 엘엘씨 | Aad-1 이벤트 das-40278-9, 관련 트랜스제닉 옥수수 식물주, 및 그의 이벤트-특이적 확인 |
US8598413B2 (en) * | 2009-08-19 | 2013-12-03 | Dow AgroSciecnes, LLC. | AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1 |
UA111936C2 (uk) | 2009-12-16 | 2016-07-11 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | ТРАНСГЕННА РОСЛИНА, ЯКА МІСТИТЬ ДНК, ЩО КОДУЄ ІНСЕКТИЦИДНИЙ БІЛОК Vip3Ab, І ДНК, ЩО КОДУЄ ІНСЕКТИЦИДНИЙ БІЛОК Cry1Ca, ДЛЯ КЕРУВАННЯ РЕЗИСТЕНТНІСТЮ КОМАХ |
CN102892284B (zh) * | 2009-12-17 | 2016-03-23 | 先锋国际良种公司 | 玉米事件dp-004114-3及其检测方法 |
UA114716C2 (uk) * | 2011-07-13 | 2017-07-25 | Дау Аґросаєнсиз Елелсі | Трасгенна рослина сої, стійка до гербіцидів |
CN102559717B (zh) * | 2012-01-05 | 2013-03-06 | 华中农业大学 | 一种抗寒的草铵膦抗性基因及应用 |
CN104219950B (zh) | 2012-02-01 | 2018-02-06 | 陶氏益农公司 | 人造的芸苔属衍生的叶绿体转运肽 |
CN103320433A (zh) * | 2013-06-06 | 2013-09-25 | 中国科学院广州生物医药与健康研究院 | 一种用于高效构建无抗性标记重组分枝杆菌的抗性表达盒 |
CA2926197A1 (en) | 2013-10-04 | 2015-04-09 | Dow Agrosciences Llc | Zea mays metallothionein-like regulatory elements and uses thereof |
BR102014025499A2 (pt) | 2013-10-15 | 2015-09-29 | Dow Agrosciences Llc | elementos regulatórios de zea mays e uso dos mesmos |
RU2659002C1 (ru) | 2013-12-10 | 2018-06-26 | ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи | Синергетическая борьба с сорняками с помощью гербицидов и улучшенная устойчивость сельскохозяйственной культуры при использовании комбинаций, включающих 2,4-d-холин и глуфосинат, в устойчивых по отношению к 2,4-d-холину и глуфосинату сое, кукурузе, хлопчатнике |
WO2015094884A1 (en) | 2013-12-20 | 2015-06-25 | Dow Agrosciences Llc | Synergistic herbicidal weed control |
AR100785A1 (es) | 2014-06-09 | 2016-11-02 | Dow Agrosciences Llc | Control herbicida de maleza a partir de combinaciones de fluroxipir e inhibidores de als |
AR102333A1 (es) | 2014-08-19 | 2017-02-22 | Regeneron Pharma | Selectividad eficiente de proteínas recombinadas |
US10947556B2 (en) | 2014-12-19 | 2021-03-16 | AgBiome, Inc. | Sequences to facilitate incorporation of DNA into the genome of an organism |
BR102016012010A2 (pt) | 2015-05-29 | 2020-03-24 | Dow Agrosciences Llc | Molécula de ácido nucleico, de ácido ribonucleico (rna) e de ácido ribonucleico de filamento duplo (dsrna), usos de célula, planta e semente, produto primário, bem como métodos para controlar uma população de pragas coleópteras e/ou hemípteras, para melhorar o rendimento de uma cultura, e para produzir uma célula vegetal transgênica e uma planta transgênica |
WO2018136547A1 (en) * | 2017-01-17 | 2018-07-26 | Noble Research Institute, Llc | Dna-free genome editing and selection methods in plants |
CR20200062A (es) * | 2017-08-09 | 2020-02-26 | Basf Se | Mezclas herbicidas que comprenden l-glufosinato, o sus sales, y al menos un inhibidor de protoporfirinógeno-ix oxidasa |
CN107513521A (zh) * | 2017-09-02 | 2017-12-26 | 中国农业科学院生物技术研究所 | 杂交瘤细胞株、所分泌的单克隆抗体及其在检测bar/PAT蛋白中的应用 |
US11920141B2 (en) * | 2021-09-24 | 2024-03-05 | Oms Investments, Inc. | Glufosinate resistance cassettes and plants comprising the same |
WO2023222226A1 (en) | 2022-05-19 | 2023-11-23 | Evonik Operations Gmbh | Enzymatic method for producing l-glufosinate |
WO2023222227A1 (en) | 2022-05-19 | 2023-11-23 | Evonik Operations Gmbh | Enzymatic method for producing l-glufosinate |
CN117470823A (zh) * | 2023-11-16 | 2024-01-30 | 河北科技大学 | 一种铜绿微囊藻荧光检测草铵膦毒性的方法 |
Family Cites Families (21)
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US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
EP0242236B2 (en) | 1986-03-11 | 1996-08-21 | Plant Genetic Systems N.V. | Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering |
US5135867A (en) | 1988-11-01 | 1992-08-04 | Mycogen Corporation | Gene encoding a lepidopteran-active toxin from Bacillus thuringiensis isolate denoted B.t. .PS81GG active against lepidopteran pests |
US5767367A (en) * | 1990-06-23 | 1998-06-16 | Hoechst Aktiengesellschaft | Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation |
DK0536330T3 (da) | 1990-06-25 | 2002-04-22 | Monsanto Technology Llc | Glyphosattolerante planter |
US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
FR2751347B1 (fr) * | 1996-07-16 | 2001-12-07 | Rhone Poulenc Agrochimie | Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides |
US20030041357A1 (en) | 1996-11-07 | 2003-02-27 | Zeneca Limited | Herbicide resistant plants |
US6376754B1 (en) * | 1997-03-07 | 2002-04-23 | Asgrow Seed Company | Plants having resistance to multiple herbicides and its use |
ZA986574B (en) * | 1997-07-23 | 2000-01-12 | Sanford Scient Inc | Improved plastid transformation of higher plants and production of transgenic plants with herbicide resistance. |
AU5482299A (en) * | 1998-08-12 | 2000-03-06 | Maxygen, Inc. | Dna shuffling to produce herbicide selective crops |
WO2000066746A1 (en) * | 1999-04-29 | 2000-11-09 | Syngenta Limited | Herbicide resistant plants |
CZ20013856A3 (cs) * | 1999-04-29 | 2002-04-17 | Syngenta Ltd. | Herbicidně rezistentní rostliny |
CN1360632A (zh) * | 1999-05-04 | 2002-07-24 | 孟山都技术有限公司 | 鞘翅目毒性多肽组合物和抗虫转基因植物 |
DE10102338A1 (de) * | 2001-01-19 | 2002-07-25 | Basf Plant Science Gmbh | Verfahren zur Expression von Biosynthesegenen in pflanzlichen Samen unter Verwendung von neuen multiplen Expressionskonstrukten |
EP2308977B2 (en) | 2004-04-30 | 2017-04-26 | Dow AgroSciences LLC | Novel herbicide resistance gene |
CA2586241C (en) * | 2004-11-17 | 2013-07-30 | Hokko Chemical Industry Co., Ltd. | Herbicide-resistance gene and utilization thereof |
CA2608717A1 (en) | 2005-05-18 | 2006-11-23 | The Board Of Trustees Operating Michigan State University | Resistance to soybean aphid in early maturing soybean germplasm |
CN103361316B (zh) | 2005-10-28 | 2017-05-17 | 美国陶氏益农公司 | 新除草剂抗性基因 |
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2007
- 2007-12-07 ES ES07869044.3T patent/ES2553331T3/es active Active
- 2007-12-07 PT PT131607632T patent/PT2610334T/pt unknown
- 2007-12-07 NZ NZ577363A patent/NZ577363A/en unknown
- 2007-12-07 AU AU2007329208A patent/AU2007329208B2/en not_active Ceased
- 2007-12-07 DK DK13160763.2T patent/DK2610334T3/en active
- 2007-12-07 ES ES13160763.2T patent/ES2609848T3/es active Active
- 2007-12-07 CN CN201710233286.1A patent/CN106987561A/zh active Pending
- 2007-12-07 MX MX2009006093A patent/MX2009006093A/es active IP Right Grant
- 2007-12-07 CA CA2671365A patent/CA2671365C/en active Active
- 2007-12-07 HU HUE13160763A patent/HUE031454T2/en unknown
- 2007-12-07 SI SI200731882A patent/SI2610334T1/sl unknown
- 2007-12-07 DK DK07869044.3T patent/DK2094831T3/en active
- 2007-12-07 CN CN200780051075.0A patent/CN101600790B/zh active Active
- 2007-12-07 BR BRPI0720226A patent/BRPI0720226B8/pt active IP Right Grant
- 2007-12-07 EP EP07869044.3A patent/EP2094831B1/en active Active
- 2007-12-07 JP JP2009540502A patent/JP5563825B2/ja not_active Expired - Fee Related
- 2007-12-07 CN CN202010671836.XA patent/CN111944759A/zh active Pending
- 2007-12-07 WO PCT/US2007/086813 patent/WO2008070845A2/en active Application Filing
- 2007-12-07 PL PL13160763T patent/PL2610334T3/pl unknown
- 2007-12-07 EP EP13160763.2A patent/EP2610334B1/en active Active
- 2007-12-07 HU HUE07869044A patent/HUE026199T2/en unknown
- 2007-12-07 US US12/517,906 patent/US10752913B2/en active Active
- 2007-12-07 AR ARP070105500A patent/AR064200A1/es active IP Right Grant
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2017
- 2017-09-26 AR ARP170102654A patent/AR109558A2/es unknown
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2020
- 2020-08-20 US US16/998,354 patent/US20200385752A1/en active Pending
- 2020-08-20 US US16/998,329 patent/US20200385751A1/en not_active Abandoned
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