ES2539105T3 - Células mesenquimatosas y osteoblastos procedentes de células madre embrionarias humanas - Google Patents
Células mesenquimatosas y osteoblastos procedentes de células madre embrionarias humanas Download PDFInfo
- Publication number
- ES2539105T3 ES2539105T3 ES02756367.5T ES02756367T ES2539105T3 ES 2539105 T3 ES2539105 T3 ES 2539105T3 ES 02756367 T ES02756367 T ES 02756367T ES 2539105 T3 ES2539105 T3 ES 2539105T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- embryonic stem
- human embryonic
- mesenchymal
- osteoblast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000000963 osteoblast Anatomy 0.000 title abstract description 7
- 210000001671 embryonic stem cell Anatomy 0.000 title abstract 2
- 239000001963 growth medium Substances 0.000 abstract description 8
- 102100024505 Bone morphogenetic protein 4 Human genes 0.000 abstract description 2
- 229960005070 ascorbic acid Drugs 0.000 abstract description 2
- 239000011668 ascorbic acid Substances 0.000 abstract description 2
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 abstract description 2
- 229960003957 dexamethasone Drugs 0.000 abstract description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 2
- 101000762379 Homo sapiens Bone morphogenetic protein 4 Proteins 0.000 abstract 1
- 235000010323 ascorbic acid Nutrition 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 34
- 102100021579 Enhancer of filamentation 1 Human genes 0.000 description 8
- 101800000791 Hemagglutinin-esterase-fusion glycoprotein chain 1 Proteins 0.000 description 8
- 101000655352 Homo sapiens Telomerase reverse transcriptase Proteins 0.000 description 8
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
- 108010017842 Telomerase Proteins 0.000 description 4
- 241000283707 Capra Species 0.000 description 3
- 229920000209 Hexadimethrine bromide Polymers 0.000 description 3
- 102000004067 Osteocalcin Human genes 0.000 description 3
- 108090000573 Osteocalcin Proteins 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229950010131 puromycin Drugs 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000001143 conditioned effect Effects 0.000 description 2
- 108010082117 matrigel Proteins 0.000 description 2
- 230000001177 retroviral effect Effects 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241001430294 unidentified retrovirus Species 0.000 description 2
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 description 1
- 108010049955 Bone Morphogenetic Protein 4 Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108060003393 Granulin Proteins 0.000 description 1
- 101001094700 Homo sapiens POU domain, class 5, transcription factor 1 Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/14—Calcium; Ca chelators; Calcitonin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/44—Thiols, e.g. mercaptoethanol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/04—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from germ cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Rheumatology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Orthopedic Medicine & Surgery (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Physical Education & Sports Medicine (AREA)
- Developmental Biology & Embryology (AREA)
- Neurology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US30373201P | 2001-07-06 | 2001-07-06 | |
| US303732P | 2001-07-06 | ||
| PCT/US2002/020998 WO2003004605A2 (en) | 2001-07-06 | 2002-07-03 | Mesenchymal cells and osteoblasts from human embryonic stem cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2539105T3 true ES2539105T3 (es) | 2015-06-26 |
Family
ID=23173436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES02756367.5T Expired - Lifetime ES2539105T3 (es) | 2001-07-06 | 2002-07-03 | Células mesenquimatosas y osteoblastos procedentes de células madre embrionarias humanas |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US20030021771A1 (enExample) |
| EP (1) | EP1412481B1 (enExample) |
| JP (1) | JP2004535808A (enExample) |
| CN (2) | CN1636054A (enExample) |
| AU (1) | AU2002322379B2 (enExample) |
| CA (1) | CA2453068C (enExample) |
| ES (1) | ES2539105T3 (enExample) |
| GB (1) | GB2392674B (enExample) |
| IL (2) | IL159578A0 (enExample) |
| WO (1) | WO2003004605A2 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7410798B2 (en) | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| CA2435826A1 (en) * | 2001-01-24 | 2002-08-01 | The Government Of The United States Of America | Differentiation of stem cells to pancreatic endocrine cells |
| US20030211605A1 (en) * | 2001-05-01 | 2003-11-13 | Lee Sang-Hun | Derivation of midbrain dopaminergic neurons from embryonic stem cells |
| GB0222846D0 (en) | 2002-10-03 | 2002-11-06 | Choo Yen | Cell culture |
| PT1572984E (pt) | 2002-12-16 | 2016-06-03 | Technion Res & Dev Foundation | Sistema de cultura para células estaminais embrionárias humanas sem células alimentadoras e sem xeno |
| US20030224411A1 (en) * | 2003-03-13 | 2003-12-04 | Stanton Lawrence W. | Genes that are up- or down-regulated during differentiation of human embryonic stem cells |
| JP2005304443A (ja) * | 2004-04-26 | 2005-11-04 | Institute Of Physical & Chemical Research | 前駆間葉系幹細胞 |
| US7592176B2 (en) * | 2004-05-07 | 2009-09-22 | Wisconsin Alumni Research Foundation | Method of forming mesenchymal stem cells from embryonic stem cells |
| WO2005113751A1 (en) * | 2004-05-14 | 2005-12-01 | Becton, Dickinson And Company | Cell culture environments for the serum-free expansion of mesenchymal stem cells |
| AU2005271723B2 (en) | 2004-07-13 | 2010-12-16 | Asterias Biotherapeutics, Inc. | Medium for growing human embryonic stem cells |
| US8367405B2 (en) | 2005-04-12 | 2013-02-05 | Mesoblast, Inc. | Isolation of adult multipotential cells by tissue non-specific alkaline phosphatase |
| US9074181B2 (en) | 2005-06-22 | 2015-07-07 | Asterias Biotherapeutics, Inc. | Suspension culture of human embryonic stem cells |
| WO2007013430A1 (ja) * | 2005-07-29 | 2007-02-01 | Matsumoto Dental University | 歯の再生方法 |
| WO2007026353A2 (en) | 2005-08-29 | 2007-03-08 | Technion Research & Development Foundation Ltd. | Media for culturing stem cells |
| WO2008020815A1 (en) * | 2006-08-15 | 2008-02-21 | Agency For Science, Technology And Research | Mesenchymal stem cell conditioned medium |
| WO2007027157A1 (en) * | 2005-09-02 | 2007-03-08 | Agency For Science, Technology And Research | Method of deriving progenitor cell line |
| GB0526664D0 (en) * | 2005-11-30 | 2006-02-08 | Plasticell Ltd | Method |
| US8241902B2 (en) * | 2006-01-11 | 2012-08-14 | Technion Research & Development Foundation Ltd. | Preparation of adult stem cell-derived connective tissue progenitors |
| PL1989295T3 (pl) | 2006-02-16 | 2021-02-22 | Université Libre de Bruxelles | Różnicowanie osteogenne komórek macierzystych szpiku kostnego oraz komórki i populacje osteoprogenitorowe lub osteoblastyczne |
| WO2007122233A1 (en) * | 2006-04-25 | 2007-11-01 | Vrije Universiteit Brussel | Preparation of mesenchymal progenitor cells, particularly osteogenic progenitor cells |
| DK2733203T3 (en) | 2006-08-02 | 2019-02-04 | Technion Res & Dev Foundation | PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE |
| AU2007304423A1 (en) * | 2006-10-02 | 2008-04-10 | Cellartis Ab | Novel toxicity assay based on human blastocyst-derived stem cells and progenitor cells |
| KR100937456B1 (ko) * | 2007-08-01 | 2010-01-19 | 한국생명공학연구원 | 인간배아줄기세포를 조골세포 직계열로 분화시키는 방법 |
| WO2010105311A1 (en) * | 2009-03-20 | 2010-09-23 | Angioblast Systems, Inc. | Production of reprogrammed pluripotent cells |
| CA2766164A1 (en) * | 2009-06-25 | 2010-12-29 | Geron Corporation | Differentiated pluripotent stem cell progeny depleted of extraneous phenotypes |
| KR101135636B1 (ko) * | 2009-10-27 | 2012-04-17 | 서울대학교산학협력단 | 인간 만능줄기세포로부터 중배엽 줄기세포를 생산하는 방법 및 이에 의해 생성된 중배엽 줄기세포 |
| WO2011058558A2 (en) | 2009-11-12 | 2011-05-19 | Technion Research & Development Foundation Ltd. | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| WO2011124741A1 (es) * | 2010-04-08 | 2011-10-13 | Fundación Progreso Y Salud | Uso de un medio de cultivo condicionado por células madre mesenquimales para la diferenciación de células madre pluripotentes humanas |
| EP2556147B1 (en) | 2010-04-08 | 2018-10-03 | The University Court Of The University of Edinburgh | Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof |
| WO2011159797A2 (en) | 2010-06-15 | 2011-12-22 | Cellular Dynamics International, Inc. | A compendium of ready-built stem cell models for interrogation of biological response |
| CA2829804A1 (en) | 2011-03-29 | 2012-10-04 | Geron Corporation | Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells |
| CN103083653B (zh) * | 2011-10-28 | 2015-07-22 | 辽宁成大动物药业有限公司 | 一种采用微载体高密度细胞培养技术生产猪瘟活疫苗的方法 |
| CN103777009B (zh) * | 2012-10-18 | 2016-03-02 | 辽宁成大动物药业有限公司 | 一种使用辣根过氧化物酶标抗体检测猪瘟弱毒病毒滴度的方法 |
| KR20160145186A (ko) | 2014-04-24 | 2016-12-19 | 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 | 입양 세포 요법 생성물을 생성하기 위한 유도 만능 줄기 세포의 응용 |
| US11285177B2 (en) | 2018-01-03 | 2022-03-29 | Globus Medical, Inc. | Allografts containing viable cells and methods thereof |
| CN108570448B (zh) * | 2018-01-26 | 2019-04-02 | 皓昇莱生物制药有限公司 | 一种高效的hPSCs向MSCs分化的方法 |
| KR102241185B1 (ko) * | 2019-04-23 | 2021-04-16 | (주)셀라토즈테라퓨틱스 | 근골격계 줄기세포의 선택적 분화 조절방법 |
| CN113462642A (zh) * | 2021-08-12 | 2021-10-01 | 呈诺再生医学科技(珠海横琴新区)有限公司 | 间充质干细胞的快速诱导分化方法、试剂盒及其应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5486359A (en) * | 1990-11-16 | 1996-01-23 | Osiris Therapeutics, Inc. | Human mesenchymal stem cells |
| US5654186A (en) * | 1993-02-26 | 1997-08-05 | The Picower Institute For Medical Research | Blood-borne mesenchymal cells |
| US5681701A (en) * | 1993-07-12 | 1997-10-28 | Mayo Foundation For Medical Education And Research | Immortalized human fetal osteoblastic cells |
| US5691175A (en) * | 1993-07-12 | 1997-11-25 | Mayo Foundation For Medical Education And Research | Immortalized human fetal osteoblastic cells |
| US5972703A (en) * | 1994-08-12 | 1999-10-26 | The Regents Of The University Of Michigan | Bone precursor cells: compositions and methods |
| US5843780A (en) * | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| GB9516268D0 (en) * | 1995-08-08 | 1995-10-11 | Danbiosyst Uk | Compositiion for enhanced uptake of polar drugs from the colon |
| CA2237890C (en) * | 1995-11-16 | 2011-03-29 | Case Western Reserve University | In vitro chondrogenic induction of human mesenchymal stem cells |
| US6482231B1 (en) * | 1995-11-20 | 2002-11-19 | Giovanni Abatangelo | Biological material for the repair of connective tissue defects comprising mesenchymal stem cells and hyaluronic acid derivative |
| US6200606B1 (en) * | 1996-01-16 | 2001-03-13 | Depuy Orthopaedics, Inc. | Isolation of precursor cells from hematopoietic and nonhematopoietic tissues and their use in vivo bone and cartilage regeneration |
| US6090622A (en) * | 1997-03-31 | 2000-07-18 | The Johns Hopkins School Of Medicine | Human embryonic pluripotent germ cells |
| US6077987A (en) * | 1997-09-04 | 2000-06-20 | North Shore-Long Island Jewish Research Institute | Genetic engineering of cells to enhance healing and tissue regeneration |
| WO1999020740A2 (en) * | 1997-10-23 | 1999-04-29 | Geron Corporation | Methods and materials for the growth of primate-derived primordial stem cells |
| US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| EP1129176A4 (en) * | 1998-11-09 | 2002-10-30 | Es Cell Int Pte Ltd | EMBRYONIC STEM CELLS |
| CA2379683A1 (en) * | 1999-07-20 | 2001-01-25 | University Of Southern California | Identification of pluripotent pre-mesenchymal, pre-hematopoietic progenitor cell |
| JP2003512052A (ja) * | 1999-10-15 | 2003-04-02 | アドバンスド セル テクノロジー、インコーポレイテッド | 分化前駆細胞及び系統欠失胚性幹細胞の産生方法 |
| WO2001035968A1 (en) * | 1999-11-19 | 2001-05-25 | Children's Medical Center Corporation | Methods for inducing chondrogenesis and producing de novo cartilage in vitro |
| GB0003930D0 (en) * | 2000-02-18 | 2000-04-12 | King S College London | Cell |
| US20040224403A1 (en) * | 2001-12-07 | 2004-11-11 | Robarts Research Institute | Reconstituting hematopoietic cell function using human embryonic stem cells |
-
2002
- 2002-07-03 IL IL15957802A patent/IL159578A0/xx unknown
- 2002-07-03 US US10/189,154 patent/US20030021771A1/en not_active Abandoned
- 2002-07-03 CN CNA028135555A patent/CN1636054A/zh active Pending
- 2002-07-03 GB GB0400481A patent/GB2392674B/en not_active Expired - Fee Related
- 2002-07-03 WO PCT/US2002/020998 patent/WO2003004605A2/en not_active Ceased
- 2002-07-03 CA CA2453068A patent/CA2453068C/en not_active Expired - Lifetime
- 2002-07-03 AU AU2002322379A patent/AU2002322379B2/en not_active Ceased
- 2002-07-03 JP JP2003510764A patent/JP2004535808A/ja active Pending
- 2002-07-03 EP EP02756367.5A patent/EP1412481B1/en not_active Expired - Lifetime
- 2002-07-03 ES ES02756367.5T patent/ES2539105T3/es not_active Expired - Lifetime
- 2002-07-03 US US10/189,276 patent/US20030036194A1/en not_active Abandoned
- 2002-07-03 CN CN200910152133.XA patent/CN101696397B/zh not_active Expired - Fee Related
-
2003
- 2003-12-25 IL IL159578A patent/IL159578A/en unknown
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2005
- 2005-08-16 US US11/205,433 patent/US20050282274A1/en not_active Abandoned
- 2005-10-17 US US11/252,467 patent/US20060057720A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2453068C (en) | 2018-02-20 |
| WO2003004605A3 (en) | 2003-11-13 |
| IL159578A0 (en) | 2004-06-01 |
| EP1412481A2 (en) | 2004-04-28 |
| US20060057720A1 (en) | 2006-03-16 |
| GB2392674B (en) | 2005-08-10 |
| CN101696397A (zh) | 2010-04-21 |
| GB0400481D0 (en) | 2004-02-11 |
| WO2003004605A2 (en) | 2003-01-16 |
| US20050282274A1 (en) | 2005-12-22 |
| EP1412481B1 (en) | 2015-04-01 |
| IL159578A (en) | 2010-11-30 |
| US20030036194A1 (en) | 2003-02-20 |
| CA2453068A1 (en) | 2003-01-16 |
| HK1141552A1 (en) | 2010-11-12 |
| EP1412481A4 (en) | 2005-01-05 |
| JP2004535808A (ja) | 2004-12-02 |
| CN1636054A (zh) | 2005-07-06 |
| US20030021771A1 (en) | 2003-01-30 |
| AU2002322379B2 (en) | 2007-02-15 |
| GB2392674A (en) | 2004-03-10 |
| CN101696397B (zh) | 2015-07-08 |
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