JP2004535808A - ヒト胚幹細胞由来の間葉性細胞および骨芽細胞 - Google Patents

ヒト胚幹細胞由来の間葉性細胞および骨芽細胞 Download PDF

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JP2004535808A
JP2004535808A JP2003510764A JP2003510764A JP2004535808A JP 2004535808 A JP2004535808 A JP 2004535808A JP 2003510764 A JP2003510764 A JP 2003510764A JP 2003510764 A JP2003510764 A JP 2003510764A JP 2004535808 A JP2004535808 A JP 2004535808A
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チュンフイ フ
アール. スコット ティース
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ジェロン コーポレイション
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JP2003510764A 2001-07-06 2002-07-03 ヒト胚幹細胞由来の間葉性細胞および骨芽細胞 Pending JP2004535808A (ja)

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US30373201P 2001-07-06 2001-07-06
PCT/US2002/020998 WO2003004605A2 (en) 2001-07-06 2002-07-03 Mesenchymal cells and osteoblasts from human embryonic stem cell

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US (4) US20030021771A1 (enExample)
EP (1) EP1412481B1 (enExample)
JP (1) JP2004535808A (enExample)
CN (2) CN1636054A (enExample)
AU (1) AU2002322379B2 (enExample)
CA (1) CA2453068C (enExample)
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008538495A (ja) * 2005-04-12 2008-10-30 アンジオブラスト・システムズ・インコーポレーテッド 組織非特異的アルカリホスファターゼによる成体多能性細胞の単離
JP2010500047A (ja) * 2006-08-15 2010-01-07 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ 間葉系幹細胞馴化培地

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
CA2435826A1 (en) * 2001-01-24 2002-08-01 The Government Of The United States Of America Differentiation of stem cells to pancreatic endocrine cells
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells
GB0222846D0 (en) 2002-10-03 2002-11-06 Choo Yen Cell culture
PT1572984E (pt) 2002-12-16 2016-06-03 Technion Res & Dev Foundation Sistema de cultura para células estaminais embrionárias humanas sem células alimentadoras e sem xeno
US20030224411A1 (en) * 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
JP2005304443A (ja) * 2004-04-26 2005-11-04 Institute Of Physical & Chemical Research 前駆間葉系幹細胞
US7592176B2 (en) * 2004-05-07 2009-09-22 Wisconsin Alumni Research Foundation Method of forming mesenchymal stem cells from embryonic stem cells
WO2005113751A1 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Cell culture environments for the serum-free expansion of mesenchymal stem cells
AU2005271723B2 (en) 2004-07-13 2010-12-16 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
US9074181B2 (en) 2005-06-22 2015-07-07 Asterias Biotherapeutics, Inc. Suspension culture of human embryonic stem cells
WO2007013430A1 (ja) * 2005-07-29 2007-02-01 Matsumoto Dental University 歯の再生方法
WO2007026353A2 (en) 2005-08-29 2007-03-08 Technion Research & Development Foundation Ltd. Media for culturing stem cells
WO2007027157A1 (en) * 2005-09-02 2007-03-08 Agency For Science, Technology And Research Method of deriving progenitor cell line
GB0526664D0 (en) * 2005-11-30 2006-02-08 Plasticell Ltd Method
US8241902B2 (en) * 2006-01-11 2012-08-14 Technion Research & Development Foundation Ltd. Preparation of adult stem cell-derived connective tissue progenitors
PL1989295T3 (pl) 2006-02-16 2021-02-22 Université Libre de Bruxelles Różnicowanie osteogenne komórek macierzystych szpiku kostnego oraz komórki i populacje osteoprogenitorowe lub osteoblastyczne
WO2007122233A1 (en) * 2006-04-25 2007-11-01 Vrije Universiteit Brussel Preparation of mesenchymal progenitor cells, particularly osteogenic progenitor cells
DK2733203T3 (en) 2006-08-02 2019-02-04 Technion Res & Dev Foundation PROCEDURES FOR EXPANSION OF EMBRYONAL STEM CELLS IN A SUSPENSION CULTURE
AU2007304423A1 (en) * 2006-10-02 2008-04-10 Cellartis Ab Novel toxicity assay based on human blastocyst-derived stem cells and progenitor cells
KR100937456B1 (ko) * 2007-08-01 2010-01-19 한국생명공학연구원 인간배아줄기세포를 조골세포 직계열로 분화시키는 방법
WO2010105311A1 (en) * 2009-03-20 2010-09-23 Angioblast Systems, Inc. Production of reprogrammed pluripotent cells
CA2766164A1 (en) * 2009-06-25 2010-12-29 Geron Corporation Differentiated pluripotent stem cell progeny depleted of extraneous phenotypes
KR101135636B1 (ko) * 2009-10-27 2012-04-17 서울대학교산학협력단 인간 만능줄기세포로부터 중배엽 줄기세포를 생산하는 방법 및 이에 의해 생성된 중배엽 줄기세포
WO2011058558A2 (en) 2009-11-12 2011-05-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2011124741A1 (es) * 2010-04-08 2011-10-13 Fundación Progreso Y Salud Uso de un medio de cultivo condicionado por células madre mesenquimales para la diferenciación de células madre pluripotentes humanas
EP2556147B1 (en) 2010-04-08 2018-10-03 The University Court Of The University of Edinburgh Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof
WO2011159797A2 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. A compendium of ready-built stem cell models for interrogation of biological response
CA2829804A1 (en) 2011-03-29 2012-10-04 Geron Corporation Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells
CN103083653B (zh) * 2011-10-28 2015-07-22 辽宁成大动物药业有限公司 一种采用微载体高密度细胞培养技术生产猪瘟活疫苗的方法
CN103777009B (zh) * 2012-10-18 2016-03-02 辽宁成大动物药业有限公司 一种使用辣根过氧化物酶标抗体检测猪瘟弱毒病毒滴度的方法
KR20160145186A (ko) 2014-04-24 2016-12-19 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 입양 세포 요법 생성물을 생성하기 위한 유도 만능 줄기 세포의 응용
US11285177B2 (en) 2018-01-03 2022-03-29 Globus Medical, Inc. Allografts containing viable cells and methods thereof
CN108570448B (zh) * 2018-01-26 2019-04-02 皓昇莱生物制药有限公司 一种高效的hPSCs向MSCs分化的方法
KR102241185B1 (ko) * 2019-04-23 2021-04-16 (주)셀라토즈테라퓨틱스 근골격계 줄기세포의 선택적 분화 조절방법
CN113462642A (zh) * 2021-08-12 2021-10-01 呈诺再生医学科技(珠海横琴新区)有限公司 间充质干细胞的快速诱导分化方法、试剂盒及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999020741A1 (en) * 1997-10-23 1999-04-29 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells
WO2000027995A1 (en) * 1998-11-09 2000-05-18 Monash University Embryonic stem cells
WO2001029206A1 (en) * 1999-10-15 2001-04-26 Advanced Cell Technology, Inc. Methods of producing differentiated progenitor cells and lineage-defective embryonic stem cells
WO2001035968A1 (en) * 1999-11-19 2001-05-25 Children's Medical Center Corporation Methods for inducing chondrogenesis and producing de novo cartilage in vitro

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5486359A (en) * 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5654186A (en) * 1993-02-26 1997-08-05 The Picower Institute For Medical Research Blood-borne mesenchymal cells
US5681701A (en) * 1993-07-12 1997-10-28 Mayo Foundation For Medical Education And Research Immortalized human fetal osteoblastic cells
US5691175A (en) * 1993-07-12 1997-11-25 Mayo Foundation For Medical Education And Research Immortalized human fetal osteoblastic cells
US5972703A (en) * 1994-08-12 1999-10-26 The Regents Of The University Of Michigan Bone precursor cells: compositions and methods
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
GB9516268D0 (en) * 1995-08-08 1995-10-11 Danbiosyst Uk Compositiion for enhanced uptake of polar drugs from the colon
CA2237890C (en) * 1995-11-16 2011-03-29 Case Western Reserve University In vitro chondrogenic induction of human mesenchymal stem cells
US6482231B1 (en) * 1995-11-20 2002-11-19 Giovanni Abatangelo Biological material for the repair of connective tissue defects comprising mesenchymal stem cells and hyaluronic acid derivative
US6200606B1 (en) * 1996-01-16 2001-03-13 Depuy Orthopaedics, Inc. Isolation of precursor cells from hematopoietic and nonhematopoietic tissues and their use in vivo bone and cartilage regeneration
US6090622A (en) * 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6077987A (en) * 1997-09-04 2000-06-20 North Shore-Long Island Jewish Research Institute Genetic engineering of cells to enhance healing and tissue regeneration
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
CA2379683A1 (en) * 1999-07-20 2001-01-25 University Of Southern California Identification of pluripotent pre-mesenchymal, pre-hematopoietic progenitor cell
GB0003930D0 (en) * 2000-02-18 2000-04-12 King S College London Cell
US20040224403A1 (en) * 2001-12-07 2004-11-11 Robarts Research Institute Reconstituting hematopoietic cell function using human embryonic stem cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999020741A1 (en) * 1997-10-23 1999-04-29 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells
WO2000027995A1 (en) * 1998-11-09 2000-05-18 Monash University Embryonic stem cells
WO2001029206A1 (en) * 1999-10-15 2001-04-26 Advanced Cell Technology, Inc. Methods of producing differentiated progenitor cells and lineage-defective embryonic stem cells
WO2001035968A1 (en) * 1999-11-19 2001-05-25 Children's Medical Center Corporation Methods for inducing chondrogenesis and producing de novo cartilage in vitro

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
JPN6007011866, Tissue Engineering, 200102, vol. 7, 88−99 *
JPN6007011867, Mol. Med., 2000, vol. 6, 88−95 *
JPN6007011868, Science, 1998, vol. 282, 1145−1147 *
JPN6007011869, Proc. Natl. Acad. Sci. USA, 1995, vol. 92, 7844−7848 *
JPN6010042953, J. Gene Med., 20010608, vol. 3, 240−251 *
JPN6010042954, Biochem. Biophys. Res. Commun., 200106, vol. 284, 478−484 *
JPN6010042955, Dev. Biol., 20010701, vol. 235, no. 1, 227−228 (Abstract 263) *
JPN6010043372, J. Cell. Biochem., 1997, vol. 64, 295−312 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008538495A (ja) * 2005-04-12 2008-10-30 アンジオブラスト・システムズ・インコーポレーテッド 組織非特異的アルカリホスファターゼによる成体多能性細胞の単離
JP2010500047A (ja) * 2006-08-15 2010-01-07 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ 間葉系幹細胞馴化培地

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EP1412481A2 (en) 2004-04-28
US20060057720A1 (en) 2006-03-16
GB2392674B (en) 2005-08-10
ES2539105T3 (es) 2015-06-26
CN101696397A (zh) 2010-04-21
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WO2003004605A2 (en) 2003-01-16
US20050282274A1 (en) 2005-12-22
EP1412481B1 (en) 2015-04-01
IL159578A (en) 2010-11-30
US20030036194A1 (en) 2003-02-20
CA2453068A1 (en) 2003-01-16
HK1141552A1 (en) 2010-11-12
EP1412481A4 (en) 2005-01-05
CN1636054A (zh) 2005-07-06
US20030021771A1 (en) 2003-01-30
AU2002322379B2 (en) 2007-02-15
GB2392674A (en) 2004-03-10
CN101696397B (zh) 2015-07-08

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