ES2384736T3 - Un fragmento aislado de ADN del promotor humano de SPARC y su uso - Google Patents
Un fragmento aislado de ADN del promotor humano de SPARC y su uso Download PDFInfo
- Publication number
- ES2384736T3 ES2384736T3 ES07776311T ES07776311T ES2384736T3 ES 2384736 T3 ES2384736 T3 ES 2384736T3 ES 07776311 T ES07776311 T ES 07776311T ES 07776311 T ES07776311 T ES 07776311T ES 2384736 T3 ES2384736 T3 ES 2384736T3
- Authority
- ES
- Spain
- Prior art keywords
- gene
- cells
- sequence
- dna
- promoter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000012634 fragment Substances 0.000 title description 37
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 12
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 10
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 86
- 241000701161 unidentified adenovirus Species 0.000 claims description 69
- 206010028980 Neoplasm Diseases 0.000 claims description 64
- 230000014509 gene expression Effects 0.000 claims description 55
- 239000013598 vector Substances 0.000 claims description 41
- 239000013612 plasmid Substances 0.000 claims description 25
- 239000013604 expression vector Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 16
- 238000011282 treatment Methods 0.000 claims description 16
- 108020004999 messenger RNA Proteins 0.000 claims description 15
- 230000010076 replication Effects 0.000 claims description 14
- 101710199711 Early E1A protein Proteins 0.000 claims description 11
- 230000001225 therapeutic effect Effects 0.000 claims description 10
- 206010021143 Hypoxia Diseases 0.000 claims description 9
- 230000000174 oncolytic effect Effects 0.000 claims description 9
- 230000007954 hypoxia Effects 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 7
- 230000004044 response Effects 0.000 claims description 6
- 239000013603 viral vector Substances 0.000 claims description 6
- 108020004440 Thymidine kinase Proteins 0.000 claims description 5
- 239000008194 pharmaceutical composition Substances 0.000 claims description 4
- 101100404740 Glycine max INR2 gene Proteins 0.000 claims description 3
- 230000006698 induction Effects 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 101100404734 Glycine max INR1 gene Proteins 0.000 claims description 2
- 108010063738 Interleukins Proteins 0.000 claims description 2
- 230000005855 radiation Effects 0.000 claims description 2
- 108700026220 vif Genes Proteins 0.000 claims description 2
- 102000015696 Interleukins Human genes 0.000 claims 1
- 150000003254 radicals Chemical class 0.000 claims 1
- 241001529453 unidentified herpesvirus Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 155
- 108020004414 DNA Proteins 0.000 description 80
- 241000700605 Viruses Species 0.000 description 47
- 230000000694 effects Effects 0.000 description 46
- 201000001441 melanoma Diseases 0.000 description 37
- IRSCQMHQWWYFCW-UHFFFAOYSA-N ganciclovir Chemical compound O=C1NC(N)=NC2=C1N=CN2COC(CO)CO IRSCQMHQWWYFCW-UHFFFAOYSA-N 0.000 description 30
- 229960002963 ganciclovir Drugs 0.000 description 29
- 210000002950 fibroblast Anatomy 0.000 description 24
- 210000004881 tumor cell Anatomy 0.000 description 24
- 210000001072 colon Anatomy 0.000 description 23
- 102000004190 Enzymes Human genes 0.000 description 20
- 108090000790 Enzymes Proteins 0.000 description 20
- 235000018102 proteins Nutrition 0.000 description 20
- 102000004169 proteins and genes Human genes 0.000 description 20
- 208000015181 infectious disease Diseases 0.000 description 18
- 230000003612 virological effect Effects 0.000 description 18
- 238000012360 testing method Methods 0.000 description 16
- 241001465754 Metazoa Species 0.000 description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 description 15
- 230000002101 lytic effect Effects 0.000 description 15
- 238000003556 assay Methods 0.000 description 14
- 230000003362 replicative effect Effects 0.000 description 14
- 239000002609 medium Substances 0.000 description 13
- 210000000481 breast Anatomy 0.000 description 12
- 238000001727 in vivo Methods 0.000 description 12
- 108060001084 Luciferase Proteins 0.000 description 11
- 208000029742 colonic neoplasm Diseases 0.000 description 11
- 241001135569 Human adenovirus 5 Species 0.000 description 10
- 230000000875 corresponding effect Effects 0.000 description 10
- 239000013078 crystal Substances 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 10
- 101000881168 Homo sapiens SPARC Proteins 0.000 description 9
- 230000000120 cytopathologic effect Effects 0.000 description 9
- 241000283690 Bos taurus Species 0.000 description 8
- 101100440985 Danio rerio crad gene Proteins 0.000 description 8
- 239000005089 Luciferase Substances 0.000 description 8
- 101100440987 Mus musculus Cracd gene Proteins 0.000 description 8
- 101100467905 Mus musculus Rdh16 gene Proteins 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 238000010367 cloning Methods 0.000 description 8
- 230000036961 partial effect Effects 0.000 description 8
- 229940002612 prodrug Drugs 0.000 description 8
- 239000000651 prodrug Substances 0.000 description 8
- 108091008146 restriction endonucleases Proteins 0.000 description 8
- 230000035897 transcription Effects 0.000 description 8
- 238000013518 transcription Methods 0.000 description 8
- 102100040164 ADP-ribosylation factor-binding protein GGA1 Human genes 0.000 description 7
- 206010009944 Colon cancer Diseases 0.000 description 7
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 7
- 101001037093 Homo sapiens ADP-ribosylation factor-binding protein GGA1 Proteins 0.000 description 7
- 201000011510 cancer Diseases 0.000 description 7
- 210000003679 cervix uteri Anatomy 0.000 description 7
- 239000002299 complementary DNA Substances 0.000 description 7
- 238000010276 construction Methods 0.000 description 7
- 238000012217 deletion Methods 0.000 description 7
- 230000037430 deletion Effects 0.000 description 7
- 210000002889 endothelial cell Anatomy 0.000 description 7
- 102000045436 human SPARC Human genes 0.000 description 7
- 210000002510 keratinocyte Anatomy 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 230000004614 tumor growth Effects 0.000 description 7
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000001415 gene therapy Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 239000002356 single layer Substances 0.000 description 6
- 238000010186 staining Methods 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 208000026310 Breast neoplasm Diseases 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 231100000002 MTT assay Toxicity 0.000 description 5
- 238000000134 MTT assay Methods 0.000 description 5
- 108700008625 Reporter Genes Proteins 0.000 description 5
- 108091027981 Response element Proteins 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 230000003511 endothelial effect Effects 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 238000005462 in vivo assay Methods 0.000 description 5
- 239000012212 insulator Substances 0.000 description 5
- 210000002752 melanocyte Anatomy 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 238000011144 upstream manufacturing Methods 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- 206010006187 Breast cancer Diseases 0.000 description 4
- 108700019961 Neoplasm Genes Proteins 0.000 description 4
- 102000048850 Neoplasm Genes Human genes 0.000 description 4
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 4
- 108020005202 Viral DNA Proteins 0.000 description 4
- 230000030833 cell death Effects 0.000 description 4
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 4
- 210000003038 endothelium Anatomy 0.000 description 4
- 238000000099 in vitro assay Methods 0.000 description 4
- 230000003211 malignant effect Effects 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 230000029812 viral genome replication Effects 0.000 description 4
- 102100038710 Capping protein-inhibiting regulator of actin dynamics Human genes 0.000 description 3
- 108090000331 Firefly luciferases Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101000957909 Homo sapiens Capping protein-inhibiting regulator of actin dynamics Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241001529936 Murinae Species 0.000 description 3
- 108010077077 Osteonectin Proteins 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 241000714474 Rous sarcoma virus Species 0.000 description 3
- 241000700584 Simplexvirus Species 0.000 description 3
- 102000006601 Thymidine Kinase Human genes 0.000 description 3
- 108700009124 Transcription Initiation Site Proteins 0.000 description 3
- 208000036142 Viral infection Diseases 0.000 description 3
- 230000002238 attenuated effect Effects 0.000 description 3
- 210000002449 bone cell Anatomy 0.000 description 3
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 3
- 229910000389 calcium phosphate Inorganic materials 0.000 description 3
- 239000001506 calcium phosphate Substances 0.000 description 3
- 235000011010 calcium phosphates Nutrition 0.000 description 3
- 210000000234 capsid Anatomy 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 230000006037 cell lysis Effects 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 239000003623 enhancer Substances 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000001605 fetal effect Effects 0.000 description 3
- 230000006801 homologous recombination Effects 0.000 description 3
- 238000002744 homologous recombination Methods 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 244000309459 oncolytic virus Species 0.000 description 3
- 210000000496 pancreas Anatomy 0.000 description 3
- 208000008443 pancreatic carcinoma Diseases 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000013605 shuttle vector Substances 0.000 description 3
- 210000002536 stromal cell Anatomy 0.000 description 3
- 238000010361 transduction Methods 0.000 description 3
- 230000026683 transduction Effects 0.000 description 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 230000009385 viral infection Effects 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 102100040190 ADP-ribosylation factor-binding protein GGA2 Human genes 0.000 description 2
- 101710197337 Adenovirus death protein Proteins 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101100297347 Caenorhabditis elegans pgl-3 gene Proteins 0.000 description 2
- 241000450599 DNA viruses Species 0.000 description 2
- 102100031780 Endonuclease Human genes 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 208000009889 Herpes Simplex Diseases 0.000 description 2
- 101001037082 Homo sapiens ADP-ribosylation factor-binding protein GGA2 Proteins 0.000 description 2
- 101100478240 Homo sapiens SPARC gene Proteins 0.000 description 2
- 102100021244 Integral membrane protein GPR180 Human genes 0.000 description 2
- 102000013462 Interleukin-12 Human genes 0.000 description 2
- 108010065805 Interleukin-12 Proteins 0.000 description 2
- 241000254158 Lampyridae Species 0.000 description 2
- 239000012097 Lipofectamine 2000 Substances 0.000 description 2
- 241000699660 Mus musculus Species 0.000 description 2
- 101000881173 Mus musculus SPARC Proteins 0.000 description 2
- 102000009890 Osteonectin Human genes 0.000 description 2
- 241000242739 Renilla Species 0.000 description 2
- 102100037599 SPARC Human genes 0.000 description 2
- 101150036757 SPARC gene Proteins 0.000 description 2
- 102220497176 Small vasohibin-binding protein_T47D_mutation Human genes 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 229960004150 aciclovir Drugs 0.000 description 2
- MKUXAQIIEYXACX-UHFFFAOYSA-N aciclovir Chemical compound N1C(N)=NC(=O)C2=C1N(COCCO)C=N2 MKUXAQIIEYXACX-UHFFFAOYSA-N 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000008186 active pharmaceutical agent Substances 0.000 description 2
- 150000001413 amino acids Chemical group 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000003602 anti-herpes Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 210000002403 aortic endothelial cell Anatomy 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 238000009739 binding Methods 0.000 description 2
- 230000000981 bystander Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 239000013553 cell monolayer Substances 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000002079 cooperative effect Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000003670 luciferase enzyme activity assay Methods 0.000 description 2
- 201000010225 mixed cell type cancer Diseases 0.000 description 2
- 208000029638 mixed neoplasm Diseases 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000011580 nude mouse model Methods 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 201000002528 pancreatic cancer Diseases 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000000865 phosphorylative effect Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000002685 pulmonary effect Effects 0.000 description 2
- 230000007115 recruitment Effects 0.000 description 2
- 231100000241 scar Toxicity 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 241001430294 unidentified retrovirus Species 0.000 description 2
- 210000002845 virion Anatomy 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 101800000263 Acidic protein Proteins 0.000 description 1
- 206010001497 Agitation Diseases 0.000 description 1
- 101100203828 Arabidopsis thaliana SPP3A gene Proteins 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 101000881198 Bos taurus SPARC Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 208000017897 Carcinoma of esophagus Diseases 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 102000008198 Coxsackie and Adenovirus Receptor Like Membrane Protein Human genes 0.000 description 1
- 108010035601 Coxsackie and Adenovirus Receptor Like Membrane Protein Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 208000016192 Demyelinating disease Diseases 0.000 description 1
- 206010012305 Demyelination Diseases 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 238000003718 Dual-Luciferase Reporter Assay System Methods 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241000701533 Escherichia virus T4 Species 0.000 description 1
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 208000032612 Glial tumor Diseases 0.000 description 1
- 206010018338 Glioma Diseases 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical class C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000827785 Homo sapiens Alpha-fetoprotein Proteins 0.000 description 1
- 101000848653 Homo sapiens Tripartite motif-containing protein 26 Proteins 0.000 description 1
- 241000701109 Human adenovirus 2 Species 0.000 description 1
- 241000193096 Human adenovirus B3 Species 0.000 description 1
- 241000598171 Human adenovirus sp. Species 0.000 description 1
- 241000700588 Human alphaherpesvirus 1 Species 0.000 description 1
- -1 IL -10 Proteins 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 102000000589 Interleukin-1 Human genes 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 101150008942 J gene Proteins 0.000 description 1
- 108010076502 Matrix Metalloproteinase 11 Proteins 0.000 description 1
- 102000002274 Matrix Metalloproteinases Human genes 0.000 description 1
- 108010000684 Matrix Metalloproteinases Proteins 0.000 description 1
- 208000037196 Medullary thyroid carcinoma Diseases 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010061309 Neoplasm progression Diseases 0.000 description 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 101100534124 Oryza sativa subsp. japonica SPP3 gene Proteins 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 241000254064 Photinus pyralis Species 0.000 description 1
- 101000621511 Potato virus M (strain German) RNA silencing suppressor Proteins 0.000 description 1
- 102000009572 RNA Polymerase II Human genes 0.000 description 1
- 108010009460 RNA Polymerase II Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108010052090 Renilla Luciferases Proteins 0.000 description 1
- 241000242743 Renilla reniformis Species 0.000 description 1
- 101710100111 SPARC Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 102000017303 Stromelysin-3 Human genes 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 101150003725 TK gene Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 238000010162 Tukey test Methods 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000019606 astringent taste Nutrition 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000011888 autopsy Methods 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 108010006025 bovine growth hormone Proteins 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- OQUUTERJWTYTHP-UHFFFAOYSA-N butanedioate;1h-tetrazol-1-ium Chemical compound [NH2+]1C=NN=N1.[NH2+]1C=NN=N1.[O-]C(=O)CCC([O-])=O OQUUTERJWTYTHP-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000022534 cell killing Effects 0.000 description 1
- 230000008619 cell matrix interaction Effects 0.000 description 1
- 238000001516 cell proliferation assay Methods 0.000 description 1
- 230000006364 cellular survival Effects 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 238000002784 cytotoxicity assay Methods 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 229940087451 cytovene Drugs 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940000406 drug candidate Drugs 0.000 description 1
- 238000010864 dual luciferase reporter gene assay Methods 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 201000005619 esophageal carcinoma Diseases 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 208000005017 glioblastoma Diseases 0.000 description 1
- 210000000224 granular leucocyte Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000003966 growth inhibitor Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 206010073071 hepatocellular carcinoma Diseases 0.000 description 1
- 231100000844 hepatocellular carcinoma Toxicity 0.000 description 1
- 102000046101 human AFP Human genes 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006882 induction of apoptosis Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 229940117681 interleukin-12 Drugs 0.000 description 1
- 230000002601 intratumoral effect Effects 0.000 description 1
- 230000005865 ionizing radiation Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000001325 log-rank test Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 208000029565 malignant colon neoplasm Diseases 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 208000023356 medullary thyroid gland carcinoma Diseases 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 210000002824 peroxisome Anatomy 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 108091008025 regulatory factors Proteins 0.000 description 1
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
- 230000035806 respiratory chain Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000005496 tempering Methods 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 208000013818 thyroid gland medullary carcinoma Diseases 0.000 description 1
- 229950002376 tirapazamine Drugs 0.000 description 1
- QVMPZNRFXAKISM-UHFFFAOYSA-N tirapazamine Chemical compound C1=CC=C2[N+]([O-])=NC(=N)N(O)C2=C1 QVMPZNRFXAKISM-UHFFFAOYSA-N 0.000 description 1
- 238000011200 topical administration Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- ILJSQTXMGCGYMG-UHFFFAOYSA-N triacetic acid Chemical compound CC(=O)CC(=O)CC(O)=O ILJSQTXMGCGYMG-UHFFFAOYSA-N 0.000 description 1
- 230000005751 tumor progression Effects 0.000 description 1
- 230000006490 viral transcription Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4748—Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP060101724A AR053246A1 (es) | 2006-04-28 | 2006-04-28 | Un fragmento aislado de adn del promotor humano de sparc y su uso para dirigir la expresion de un gen heterologo en celulas tumorales |
| ARP060101724 | 2006-04-28 | ||
| PCT/US2007/010196 WO2007127347A2 (en) | 2006-04-28 | 2007-04-26 | An isolated dna fragment of the sparc human promoter and its use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2384736T3 true ES2384736T3 (es) | 2012-07-11 |
Family
ID=38006653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07776311T Active ES2384736T3 (es) | 2006-04-28 | 2007-04-26 | Un fragmento aislado de ADN del promotor humano de SPARC y su uso |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8436160B2 (enExample) |
| EP (1) | EP2048954B1 (enExample) |
| JP (1) | JP5215290B2 (enExample) |
| AR (1) | AR053246A1 (enExample) |
| AT (1) | ATE550943T1 (enExample) |
| AU (1) | AU2007243252B2 (enExample) |
| ES (1) | ES2384736T3 (enExample) |
| NZ (1) | NZ572350A (enExample) |
| WO (1) | WO2007127347A2 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2304281B1 (es) * | 2006-02-01 | 2009-08-12 | Dnatrix Inc. | Adenovirus oncoliticos para el tratamiento del cancer. |
| US9056133B2 (en) * | 2011-05-25 | 2015-06-16 | Cti-S.A. | Pharmaceutical kit and method for treating cancer |
| US11542526B2 (en) | 2019-02-21 | 2023-01-03 | Unleash Immuno Oncolytics, Inc. | Oncolytic adenoviral vector and methods of use |
| CA3183673A1 (en) | 2020-06-26 | 2021-12-30 | Robert Allen | Oncolytic herpes simplex viruses (hsv) expressing immunomodulatory fusion proteins |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5772993A (en) * | 1997-01-21 | 1998-06-30 | The University Of Virginia Patent Foundation | Osteocalcin promoter-based toxic gene therapy for the treatment of calcified tumors and tissues |
| IL146794A0 (en) * | 1999-05-28 | 2002-07-25 | Univ Virginia | Osteonectin based toxic gene therapy for the treatment of calcified tumors and tissues |
| US7563748B2 (en) | 2003-06-23 | 2009-07-21 | Cognis Ip Management Gmbh | Alcohol alkoxylate carriers for pesticide active ingredients |
| US7879542B2 (en) * | 2003-06-24 | 2011-02-01 | The Johns Hopkins University | Methylated gene biomarkers for detecting cancer |
| ATE498358T1 (de) | 2005-06-29 | 2011-03-15 | Compumedics Ltd | Sensoranordnung mit leitfähiger brücke |
-
2006
- 2006-04-28 AR ARP060101724A patent/AR053246A1/es active IP Right Grant
-
2007
- 2007-04-26 WO PCT/US2007/010196 patent/WO2007127347A2/en not_active Ceased
- 2007-04-26 US US12/298,743 patent/US8436160B2/en active Active - Reinstated
- 2007-04-26 EP EP07776311A patent/EP2048954B1/en not_active Not-in-force
- 2007-04-26 AT AT07776311T patent/ATE550943T1/de active
- 2007-04-26 NZ NZ572350A patent/NZ572350A/en not_active IP Right Cessation
- 2007-04-26 ES ES07776311T patent/ES2384736T3/es active Active
- 2007-04-26 AU AU2007243252A patent/AU2007243252B2/en not_active Ceased
- 2007-04-26 JP JP2009507816A patent/JP5215290B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007127347A3 (en) | 2008-01-03 |
| AU2007243252B2 (en) | 2013-06-20 |
| EP2048954A2 (en) | 2009-04-22 |
| JP5215290B2 (ja) | 2013-06-19 |
| NZ572350A (en) | 2011-12-22 |
| JP2009535031A (ja) | 2009-10-01 |
| US8436160B2 (en) | 2013-05-07 |
| EP2048954B1 (en) | 2012-03-28 |
| AR053246A1 (es) | 2007-04-25 |
| WO2007127347A2 (en) | 2007-11-08 |
| EP2048954A4 (en) | 2009-12-02 |
| ATE550943T1 (de) | 2012-04-15 |
| US20090312401A1 (en) | 2009-12-17 |
| AU2007243252A1 (en) | 2007-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102779944B1 (ko) | 키메라 수용체 및 그의 사용 방법 | |
| JP4981229B2 (ja) | ハイブリッド血管内皮成長因子ＤＮＡｓおよびタンパク質に関与する物質および方法 | |
| US7563448B2 (en) | Modified vaccinia ankara expressing P53 in cancer immunotherapy | |
| KR20220090512A (ko) | 액체암의 치료를 위한 조성물 및 방법 | |
| KR20220038362A (ko) | 재조합 ad35 벡터 및 관련 유전자 요법 개선 | |
| JPH10503361A (ja) | 組換えｐ５３アデノウイルス方法と組成物 | |
| KR20190138311A (ko) | 종양용해성 바이러스요법 및 면역요법 | |
| KR20210151785A (ko) | 비바이러스성 dna 벡터 및 fviii 치료제 발현을 위한 이의 용도 | |
| US20240207318A1 (en) | Chimeric costimulatory receptors, chemokine receptors, and the use of same in cellular immunotherapies | |
| CN116887820A (zh) | 腺病毒载体以及关于使用腺病毒载体的方法 | |
| KR20100049084A (ko) | 질병 진단을 위한 방법 및 조성물 | |
| CN114835821B (zh) | 一种高效特异实现碱基颠换的编辑系统、方法及用途 | |
| KR20230003511A (ko) | 안면견갑상완 근이영양증에 대한 crispr-억제 | |
| JP7667595B2 (ja) | Ａｑｐ１ ＲＮＡを標的とするｓｇＲＮＡ及びそのベクターと使用 | |
| Huang et al. | A variant form of ETS1 induces apoptosis in human colon cancer cells | |
| CN113454103B (zh) | 紫外线诱导dna损伤的修复 | |
| Milas et al. | Adenovirus-mediated p53 gene therapy inhibits human sarcoma tumorigenicity | |
| AU782255B2 (en) | Gene transfer vectors for treating autoimmune diseases and diseases with immunopathogenesis by therapy | |
| DK2948157T3 (en) | APPLICATION OF A RE-MODIFIED INFECTIOUS MEASURED VIRUS WITH IMPROVED PROAPOPTOTIC PROPERTIES (MV DELTAC VIRUS) FOR CANCER THERAPY | |
| US6200799B1 (en) | Somatic gene therapy to suppress secondary cataract formation following eye surgery | |
| EA009388B1 (ru) | Векторы экспрессии и способы их применения | |
| JP2003523722A (ja) | 抗新生物性組成物およびその使用 | |
| EP2048954B1 (en) | An isolated dna fragment of the sparc human promoter and its use | |
| US20260021181A1 (en) | Chimeric costimulatory receptors, chemokine receptors, and the use of same in cellular immunotherapies | |
| US7053194B2 (en) | Compositions and methods for p53-mediated repression of gene expression |