ES2262180T3 - Il12 para la terapia genetica de tumores. - Google Patents
Il12 para la terapia genetica de tumores.Info
- Publication number
- ES2262180T3 ES2262180T3 ES97927949T ES97927949T ES2262180T3 ES 2262180 T3 ES2262180 T3 ES 2262180T3 ES 97927949 T ES97927949 T ES 97927949T ES 97927949 T ES97927949 T ES 97927949T ES 2262180 T3 ES2262180 T3 ES 2262180T3
- Authority
- ES
- Spain
- Prior art keywords
- baselineskip
- tumor
- cells
- construction
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 130
- 108010065805 Interleukin-12 Proteins 0.000 title claims abstract description 120
- 102000013462 Interleukin-12 Human genes 0.000 title claims abstract description 94
- 238000002560 therapeutic procedure Methods 0.000 title abstract description 28
- 230000002068 genetic effect Effects 0.000 title abstract description 17
- 238000010276 construction Methods 0.000 claims abstract description 40
- 238000011282 treatment Methods 0.000 claims abstract description 34
- 108090000623 proteins and genes Proteins 0.000 claims description 58
- 239000013612 plasmid Substances 0.000 claims description 42
- 239000002245 particle Substances 0.000 claims description 34
- 108020004414 DNA Proteins 0.000 claims description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 18
- 102000004169 proteins and genes Human genes 0.000 claims description 17
- 238000001727 in vivo Methods 0.000 claims description 15
- 241000124008 Mammalia Species 0.000 claims description 10
- 238000012546 transfer Methods 0.000 claims description 9
- 241000701022 Cytomegalovirus Species 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 241000699670 Mus sp. Species 0.000 description 64
- 210000004027 cell Anatomy 0.000 description 53
- 108090000663 Annexin A1 Proteins 0.000 description 19
- 102400000124 Cyclin-dependent kinase 5 activator 1, p35 Human genes 0.000 description 16
- 210000001744 T-lymphocyte Anatomy 0.000 description 15
- 230000004614 tumor growth Effects 0.000 description 15
- 230000000694 effects Effects 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 14
- 238000000034 method Methods 0.000 description 14
- 210000003491 skin Anatomy 0.000 description 14
- 210000004881 tumor cell Anatomy 0.000 description 14
- 230000014509 gene expression Effects 0.000 description 13
- 241001529936 Murinae Species 0.000 description 12
- 230000000259 anti-tumor effect Effects 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 12
- 239000013598 vector Substances 0.000 description 12
- 150000001413 amino acids Chemical class 0.000 description 11
- 238000001415 gene therapy Methods 0.000 description 11
- 238000002513 implantation Methods 0.000 description 11
- 241000699666 Mus <mouse, genus> Species 0.000 description 10
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 10
- 239000010931 gold Substances 0.000 description 10
- 229910052737 gold Inorganic materials 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 238000000338 in vitro Methods 0.000 description 9
- 230000001404 mediated effect Effects 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 238000001890 transfection Methods 0.000 description 8
- 108020004684 Internal Ribosome Entry Sites Proteins 0.000 description 7
- 210000001015 abdomen Anatomy 0.000 description 6
- 230000009885 systemic effect Effects 0.000 description 6
- 102000004127 Cytokines Human genes 0.000 description 5
- 108090000695 Cytokines Proteins 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 230000001133 acceleration Effects 0.000 description 5
- 230000028993 immune response Effects 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 230000005809 anti-tumor immunity Effects 0.000 description 4
- 230000006023 anti-tumor response Effects 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 230000008488 polyadenylation Effects 0.000 description 4
- 108091026890 Coding region Proteins 0.000 description 3
- 206010027476 Metastases Diseases 0.000 description 3
- 206010039491 Sarcoma Diseases 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 125000003275 alpha amino acid group Chemical group 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 108010006025 bovine growth hormone Proteins 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 230000002163 immunogen Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000003393 splenic effect Effects 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 206010053759 Growth retardation Diseases 0.000 description 2
- 101000694288 Homo sapiens 40S ribosomal protein SA Proteins 0.000 description 2
- 101000959738 Homo sapiens Annexin A1 Proteins 0.000 description 2
- 101000583935 Homo sapiens CDK-activating kinase assembly factor MAT1 Proteins 0.000 description 2
- 101000912009 Homo sapiens Cyclin-dependent kinase 5 activator 1 Proteins 0.000 description 2
- 101001038346 Homo sapiens GTP cyclohydrolase 1 feedback regulatory protein Proteins 0.000 description 2
- 101001090483 Homo sapiens Glutathione S-transferase LANCL1 Proteins 0.000 description 2
- 101000980900 Homo sapiens Sororin Proteins 0.000 description 2
- 101000808126 Homo sapiens Uroplakin-3b Proteins 0.000 description 2
- 102000015696 Interleukins Human genes 0.000 description 2
- 108010063738 Interleukins Proteins 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 241000237955 Nassarius Species 0.000 description 2
- 108700019961 Neoplasm Genes Proteins 0.000 description 2
- 102000048850 Neoplasm Genes Human genes 0.000 description 2
- 208000000474 Poliomyelitis Diseases 0.000 description 2
- 108010001267 Protein Subunits Proteins 0.000 description 2
- 102000002067 Protein Subunits Human genes 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
- 210000001339 epidermal cell Anatomy 0.000 description 2
- 239000001307 helium Substances 0.000 description 2
- 229910052734 helium Inorganic materials 0.000 description 2
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 2
- 102000048091 human CDCA5 Human genes 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 201000006512 mast cell neoplasm Diseases 0.000 description 2
- 208000006971 mastocytoma Diseases 0.000 description 2
- 201000001441 melanoma Diseases 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 230000001394 metastastic effect Effects 0.000 description 2
- 206010061289 metastatic neoplasm Diseases 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000001177 retroviral effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000011287 therapeutic dose Methods 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 108010062580 Concanavalin A Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
- 108010031111 EBV-encoded nuclear antigen 1 Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000710188 Encephalomyocarditis virus Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 208000001382 Experimental Melanoma Diseases 0.000 description 1
- 201000008808 Fibrosarcoma Diseases 0.000 description 1
- 101150036477 Gen gene Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 241000608571 Murina Species 0.000 description 1
- 101000959737 Mus musculus Annexin A1 Proteins 0.000 description 1
- 101000583937 Mus musculus CDK-activating kinase assembly factor MAT1 Proteins 0.000 description 1
- 101001038345 Mus musculus GTP cyclohydrolase 1 feedback regulatory protein Proteins 0.000 description 1
- 101000808124 Mus musculus Uroplakin-3b Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 208000006265 Renal cell carcinoma Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920006355 Tefzel Polymers 0.000 description 1
- 210000004241 Th2 cell Anatomy 0.000 description 1
- 241000287433 Turdus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000005975 antitumor immune response Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 238000001516 cell proliferation assay Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000016396 cytokine production Effects 0.000 description 1
- 230000007402 cytotoxic response Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008029 eradication Effects 0.000 description 1
- QHSJIZLJUFMIFP-UHFFFAOYSA-N ethene;1,1,2,2-tetrafluoroethene Chemical compound C=C.FC(F)=C(F)F QHSJIZLJUFMIFP-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000001476 gene delivery Methods 0.000 description 1
- 238000012637 gene transfection Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000000833 heterodimer Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 230000006054 immunological memory Effects 0.000 description 1
- 230000009463 immunological memory response Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229940065638 intron a Drugs 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- PWPJGUXAGUPAHP-UHFFFAOYSA-N lufenuron Chemical compound C1=C(Cl)C(OC(F)(F)C(C(F)(F)F)F)=CC(Cl)=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F PWPJGUXAGUPAHP-UHFFFAOYSA-N 0.000 description 1
- 230000015654 memory Effects 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000011049 pearl Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 208000011581 secondary neoplasm Diseases 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000007390 skin biopsy Methods 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000005740 tumor formation Effects 0.000 description 1
- 231100000402 unacceptable toxicity Toxicity 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/20—Interleukins [IL]
- A61K38/208—IL-12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US659206 | 1996-06-05 | ||
| US08/659,206 US5922685A (en) | 1996-06-05 | 1996-06-05 | IL-12 gene therapy of tumors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2262180T3 true ES2262180T3 (es) | 2006-11-16 |
Family
ID=24644498
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES97927949T Expired - Lifetime ES2262180T3 (es) | 1996-06-05 | 1997-06-04 | Il12 para la terapia genetica de tumores. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US5922685A (enExample) |
| EP (1) | EP0963207B1 (enExample) |
| JP (1) | JP2000512980A (enExample) |
| DE (1) | DE69735643T2 (enExample) |
| ES (1) | ES2262180T3 (enExample) |
| WO (1) | WO1997046263A1 (enExample) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998041229A1 (en) * | 1997-03-19 | 1998-09-24 | F. Hoffmann-La Roche Ag | USE OF IL-12p40 AS IMMUNOSTIMULANT |
| JP4336452B2 (ja) | 1997-12-08 | 2009-09-30 | メルク パテント ゲーエムベーハー | 標的化免疫治療および一般の免疫刺激に対して有用である二量体融合タンパク質 |
| US20030105294A1 (en) * | 1998-02-25 | 2003-06-05 | Stephen Gillies | Enhancing the circulating half life of antibody-based fusion proteins |
| AU758860B2 (en) * | 1998-04-15 | 2003-04-03 | Merck Patent Gmbh | Enhancement of antibody-cytokine fusion protein mediated immune responses by co-administration with angiogenesis inhibitor |
| JP2002524530A (ja) * | 1998-09-15 | 2002-08-06 | ジェネティックス・インスチチュート・インコーポレーテッド | Ｉｌ−１２を用いるカポジ肉腫の治療 |
| SK782002A3 (en) | 1999-07-21 | 2003-08-05 | Lexigen Pharm Corp | FC fusion proteins for enhancing the immunogenicity of protein and peptide antigens |
| US7067110B1 (en) | 1999-07-21 | 2006-06-27 | Emd Lexigen Research Center Corp. | Fc fusion proteins for enhancing the immunogenicity of protein and peptide antigens |
| BR0013231A (pt) * | 1999-08-09 | 2002-07-23 | Lexigen Pharm Corp | Complexos citocina-anticorpo múltiplos |
| US20030190305A1 (en) * | 1999-09-15 | 2003-10-09 | Wyeth And National Institutes Of Health | Treatment of Kaposi's sarcoma with IL-12 |
| US6802826B1 (en) | 1999-10-11 | 2004-10-12 | Felton International, Inc. | Universal anti-infectious protector for needleless injectors |
| US7074210B2 (en) * | 1999-10-11 | 2006-07-11 | Felton International, Inc. | Universal protector cap with auto-disable features for needle-free injectors |
| EP1142590B8 (en) * | 1999-10-29 | 2008-11-26 | AnGes MG, Inc. | Gene therapy for diabetic ischemic disease |
| WO2001036489A2 (en) | 1999-11-12 | 2001-05-25 | Merck Patent Gmbh | Erythropoietin forms with improved properties |
| US6770054B1 (en) | 1999-11-23 | 2004-08-03 | Felton International, Inc. | Injector assembly with driving means and locking means |
| US7887506B1 (en) | 1999-11-23 | 2011-02-15 | Pulse Needlefree Systems, Inc. | Safety mechanism to prevent accidental patient injection and methods of same |
| US7115712B1 (en) | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
| MXPA02007733A (es) | 2000-02-11 | 2004-09-10 | Merck Patent Gmbh | Mejoramiento de la vida media circulante de proteinas de fusion basadas en anticuerpos. |
| US20040213798A1 (en) * | 2000-06-08 | 2004-10-28 | Powderject Vaccines, Inc. | Spray-dried alum compositions |
| US20020120228A1 (en) * | 2000-06-08 | 2002-08-29 | Yuh-Fun Maa | Powder compositions |
| PL358582A1 (en) * | 2000-06-29 | 2004-08-09 | Merck Patent Gmbh | Enhancement of antibody-cytokine fusion protein mediated immune responses by combined treatment with immunocytokine uptake enhancing agents |
| US20020086036A1 (en) | 2000-12-05 | 2002-07-04 | Allergan Sales, Inc. | Methods for treating hyperhidrosis |
| US7255865B2 (en) * | 2000-12-05 | 2007-08-14 | Allergan, Inc. | Methods of administering botulinum toxin |
| PL206701B1 (pl) * | 2001-03-07 | 2010-09-30 | Merck Patent Gmbh | Immunoglobulinowe białko fuzyjne, kodujący je kwas nukleinowy, replikujący wektor ekspresji zawierający taki kwas nukleinowy, eukariotyczna komórka gospodarza zawierająca taki wektor ekspresji oraz sposób zwiększania ekspresji takiego białka fuzyjnego |
| WO2002079415A2 (en) * | 2001-03-30 | 2002-10-10 | Lexigen Pharmaceuticals Corp. | Reducing the immunogenicity of fusion proteins |
| KR100900166B1 (ko) * | 2001-05-03 | 2009-06-02 | 메르크 파텐트 게엠베하 | 재조합 종양 특이적 항체 및 이들의 용도 |
| CA2349506C (en) * | 2001-06-14 | 2009-12-08 | Duke University | A method for selective expression of therapeutic genes by hyperthermia |
| EP2354791A1 (en) * | 2001-12-04 | 2011-08-10 | Merck Patent GmbH | Immunocytokines with modulated selectivity |
| AU2003298187B2 (en) * | 2002-12-17 | 2010-09-16 | Merck Patent Gmbh | Humanized antibody (H14.18) of the mouse 14.18 antibody binding to GD2 and its fusion with IL-2 |
| US8420087B2 (en) * | 2004-01-05 | 2013-04-16 | Antisoma Research Limited | Interleukin-12 targeted to oncofoetal fibronectin |
| CN101351475B (zh) * | 2005-12-30 | 2013-05-15 | 默克专利有限公司 | 具有提高的稳定性的白细胞介素12p40变体 |
| US7833754B2 (en) * | 2006-01-13 | 2010-11-16 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | IL-12 for expression in mammalian cell |
| ES2636460T3 (es) * | 2007-10-08 | 2017-10-05 | Intrexon Corporation | Células dendríticas modificadas por ingeniería y usos para el tratamiento del cáncer |
| EP2356219A4 (en) | 2008-10-08 | 2017-07-05 | Intrexon Corporation | Engineered cells expressing multiple immunomodulators and uses thereof |
| WO2010126766A1 (en) * | 2009-04-30 | 2010-11-04 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Inducible interleukin-12 |
| WO2010147971A2 (en) * | 2009-06-15 | 2010-12-23 | New York University | Il23 modified viral vector for recombinant vaccines and tumor treatment |
| SG11201809381XA (en) | 2016-05-18 | 2018-12-28 | Modernatx Inc | Polynucleotides encoding interleukin-12 (il12) and uses thereof |
| JP7285220B2 (ja) | 2017-05-18 | 2023-06-01 | モデルナティエックス インコーポレイテッド | 連結したインターロイキン－１２（ｉｌ１２）ポリペプチドをコードするポリヌクレオチドを含む脂質ナノ粒子 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5833975A (en) * | 1989-03-08 | 1998-11-10 | Virogenetics Corporation | Canarypox virus expressing cytokine and/or tumor-associated antigen DNA sequence |
| US4945050A (en) * | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US5015580A (en) * | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
| US5149655A (en) * | 1990-06-21 | 1992-09-22 | Agracetus, Inc. | Apparatus for genetic transformation |
| ES2192573T3 (es) * | 1994-01-21 | 2003-10-16 | Powderject Vaccines Inc | Instrumento de deposito de genes mediante arrastre gaseoso. |
| JP2001503258A (ja) * | 1996-10-18 | 2001-03-13 | バレンティス・インコーポレーテッド | 遺伝子発現および搬送系および用途 |
-
1996
- 1996-06-05 US US08/659,206 patent/US5922685A/en not_active Expired - Fee Related
-
1997
- 1997-06-04 EP EP97927949A patent/EP0963207B1/en not_active Expired - Lifetime
- 1997-06-04 DE DE69735643T patent/DE69735643T2/de not_active Expired - Fee Related
- 1997-06-04 WO PCT/US1997/009591 patent/WO1997046263A1/en not_active Ceased
- 1997-06-04 ES ES97927949T patent/ES2262180T3/es not_active Expired - Lifetime
- 1997-06-04 JP JP10500803A patent/JP2000512980A/ja not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP0963207A1 (en) | 1999-12-15 |
| US5922685A (en) | 1999-07-13 |
| DE69735643D1 (de) | 2006-05-18 |
| HK1024622A1 (en) | 2000-10-20 |
| JP2000512980A (ja) | 2000-10-03 |
| DE69735643T2 (de) | 2006-12-14 |
| EP0963207A4 (en) | 2003-01-22 |
| WO1997046263A1 (en) | 1997-12-11 |
| EP0963207B1 (en) | 2006-04-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2262180T3 (es) | Il12 para la terapia genetica de tumores. | |
| Ashley et al. | Bone marrow–generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors | |
| EP0915708B1 (en) | Immunogenic composition comprising tumor associated antigen and human cytokine | |
| ES2382775T3 (es) | Inmunoterapia adoptiva con supervivencia de linfocitos T potenciada. | |
| Kaufman et al. | Insertion of interleukin-2 (IL-2) and interleukin-12 (IL-12) genes into vaccinia virus results in effective anti-tumor responses without toxicity | |
| JPH07503455A (ja) | 癌のリンホカイン遺伝子療法 | |
| JPH11508762A (ja) | エフェクタ細胞調節用遺伝子療法 | |
| Shi et al. | Granulocyte-macrophage colony-stimulating factor (GM-CSF) secreted by cDNA-transfected tumor cells induces a more potent antitumor response than exogenous GM-CSF | |
| CA2490863A1 (en) | Cancer vaccine containing cross-species epitopes of telomerase reverse transcriptase | |
| US20190328855A1 (en) | Optimized Synthetic Consensus Immunogenic Compositions Targeting Fibroblast Activation Protein | |
| Oshikawa et al. | Interleukin 12 gene transfer into skin distant from the tumor site elicits antimetastatic effects equivalent to local gene transfer | |
| Shawler et al. | Comparison of gene therapy with interleukin-2 gene modified fibroblasts and tumor cells in the murine CT-26 model of colorectal carcinoma | |
| EP3515466A1 (en) | Optimized synthetic consensus inmunogenic compositions targeting fibroblast activation protein | |
| Lichtor et al. | Cytokine immuno-gene therapy for treatment of brain tumors | |
| EP1513551B1 (en) | Vaccination with immuno-isolated cells producing an immunomodulator | |
| Krejci et al. | Immunotherapy for urological malignancies | |
| JP5954174B2 (ja) | 免疫誘導剤 | |
| US20050203037A1 (en) | Complex immuno-gene medical composition for inhibiting tumor cells | |
| US5935569A (en) | Genetic anticancer vaccine | |
| US20030018006A1 (en) | In vivo electroporation-mediated cytokine/immunocytokine-based antitumoral gene | |
| JP4459060B2 (ja) | ポリヌクレオチドからなるワクチン | |
| WO2022242652A1 (en) | Vaccine, use thereof and cancer vaccine cocktail | |
| HK1024622B (en) | Il 12 for the gene therapy of tumours | |
| CA3035524A1 (en) | Optimized synthetic consensus immunogenic compositions targeting the follicle stimulating hormone receptor (fshr) | |
| WO2025031347A1 (zh) | 肿瘤疫苗及其用途 |