ES2246257T3 - Procedimiento para la distincion de modificaciones de mutilacion en la posicion 5. - Google Patents
Procedimiento para la distincion de modificaciones de mutilacion en la posicion 5.Info
- Publication number
- ES2246257T3 ES2246257T3 ES00987010T ES00987010T ES2246257T3 ES 2246257 T3 ES2246257 T3 ES 2246257T3 ES 00987010 T ES00987010 T ES 00987010T ES 00987010 T ES00987010 T ES 00987010T ES 2246257 T3 ES2246257 T3 ES 2246257T3
- Authority
- ES
- Spain
- Prior art keywords
- cytosine
- sequence
- dna
- methylated
- detected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 84
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims abstract description 189
- 229940104302 cytosine Drugs 0.000 claims abstract description 93
- 108020004414 DNA Proteins 0.000 claims abstract description 79
- 230000035772 mutation Effects 0.000 claims abstract description 28
- 238000001514 detection method Methods 0.000 claims abstract description 23
- 230000011987 methylation Effects 0.000 claims abstract description 20
- 238000007069 methylation reaction Methods 0.000 claims abstract description 20
- 229940113082 thymine Drugs 0.000 claims abstract description 18
- 239000000126 substance Substances 0.000 claims abstract description 15
- 238000004458 analytical method Methods 0.000 claims abstract description 14
- 239000002773 nucleotide Substances 0.000 claims abstract description 12
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 12
- 102000054765 polymorphisms of proteins Human genes 0.000 claims abstract description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 6
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 31
- 238000006243 chemical reaction Methods 0.000 claims description 28
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 26
- 239000012634 fragment Substances 0.000 claims description 26
- 230000003321 amplification Effects 0.000 claims description 24
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 24
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 18
- 230000000295 complement effect Effects 0.000 claims description 16
- 230000008569 process Effects 0.000 claims description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 15
- 238000009396 hybridization Methods 0.000 claims description 9
- 238000005259 measurement Methods 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 8
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 claims description 7
- 238000004949 mass spectrometry Methods 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 6
- 238000012986 modification Methods 0.000 claims description 6
- 230000007758 mating behavior Effects 0.000 claims description 5
- 102000055027 Protein Methyltransferases Human genes 0.000 claims description 4
- 230000001419 dependent effect Effects 0.000 claims description 4
- 108091034117 Oligonucleotide Proteins 0.000 claims description 3
- 108700040121 Protein Methyltransferases Proteins 0.000 claims description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 3
- 230000033228 biological regulation Effects 0.000 claims description 3
- 238000005251 capillar electrophoresis Methods 0.000 claims description 3
- 238000007385 chemical modification Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 125000004122 cyclic group Chemical group 0.000 claims description 2
- 238000001962 electrophoresis Methods 0.000 claims description 2
- 239000003999 initiator Substances 0.000 claims description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical compound [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 claims description 2
- 238000002372 labelling Methods 0.000 claims 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 abstract description 6
- 108060004795 Methyltransferase Proteins 0.000 abstract description 4
- 102000016397 Methyltransferase Human genes 0.000 abstract description 4
- WBZKQQHYRPRKNJ-UHFFFAOYSA-L disulfite Chemical compound [O-]S(=O)S([O-])(=O)=O WBZKQQHYRPRKNJ-UHFFFAOYSA-L 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 23
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 9
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 6
- 239000002253 acid Substances 0.000 description 6
- 150000007513 acids Chemical class 0.000 description 6
- 230000000875 corresponding effect Effects 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- CTMZLDSMFCVUNX-VMIOUTBZSA-N cytidylyl-(3'->5')-guanosine Chemical group O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=C(C(N=C(N)N3)=O)N=C2)O)[C@@H](CO)O1 CTMZLDSMFCVUNX-VMIOUTBZSA-N 0.000 description 4
- 238000001502 gel electrophoresis Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 229940035893 uracil Drugs 0.000 description 3
- HWPZZUQOWRWFDB-UHFFFAOYSA-N 1-methylcytosine Chemical compound CN1C=CC(N)=NC1=O HWPZZUQOWRWFDB-UHFFFAOYSA-N 0.000 description 2
- 101150037478 Col11a2 gene Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 101150091617 PSMC2 gene Proteins 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000001973 epigenetic effect Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 230000007067 DNA methylation Effects 0.000 description 1
- 108010063593 DNA modification methylase SssI Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000001698 laser desorption ionisation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000037425 regulation of transcription Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus For Radiation Diagnosis (AREA)
- Mechanical Treatment Of Semiconductor (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Stereophonic System (AREA)
- Superconductors And Manufacturing Methods Therefor (AREA)
- Paper (AREA)
- Control And Other Processes For Unpacking Of Materials (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Nonmetallic Welding Materials (AREA)
- Control Of Positive-Displacement Air Blowers (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19951189 | 1999-10-15 | ||
| DE19951189A DE19951189C2 (de) | 1999-10-15 | 1999-10-15 | Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2246257T3 true ES2246257T3 (es) | 2006-02-16 |
Family
ID=7926702
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00987010T Expired - Lifetime ES2246257T3 (es) | 1999-10-15 | 2000-10-13 | Procedimiento para la distincion de modificaciones de mutilacion en la posicion 5. |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US7179594B1 (https=) |
| EP (1) | EP1228246B1 (https=) |
| JP (1) | JP4528475B2 (https=) |
| AT (1) | ATE299950T1 (https=) |
| AU (1) | AU775085B2 (https=) |
| CA (1) | CA2387148A1 (https=) |
| DE (2) | DE19951189C2 (https=) |
| DK (1) | DK1228246T3 (https=) |
| ES (1) | ES2246257T3 (https=) |
| IL (1) | IL149067A0 (https=) |
| IS (1) | IS6328A (https=) |
| NZ (1) | NZ518318A (https=) |
| WO (1) | WO2001027317A2 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19957827C2 (de) * | 1999-11-25 | 2003-06-12 | Epigenomics Ag | Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche |
| DE10010282B4 (de) * | 2000-02-25 | 2006-11-16 | Epigenomics Ag | Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| DE10151055B4 (de) * | 2001-10-05 | 2005-05-25 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln |
| DE10151069A1 (de) * | 2001-10-05 | 2003-04-30 | Epigenomics Ag | Verfahren zum Nachweis von DNA-Methylierung mittels markierten S-Adenosylmethioninanaloga |
| US6916621B2 (en) | 2002-03-27 | 2005-07-12 | Spectral Genomics, Inc. | Methods for array-based comparitive binding assays |
| US7238518B2 (en) | 2002-10-04 | 2007-07-03 | Nisshinbo Industries, Inc. | Oligonucleotide-immobilized substrate for detecting methylation |
| DE10338308B4 (de) * | 2003-08-15 | 2006-10-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in DNA |
| US20100137154A1 (en) * | 2008-12-01 | 2010-06-03 | Ach Robert A | Genome analysis using a methyltransferase |
| DE102015009187B3 (de) | 2015-07-16 | 2016-10-13 | Dimo Dietrich | Verfahren zur Bestimmung einer Mutation in genomischer DNA, Verwendung des Verfahrens und Kit zur Durchführung des Verfahrens |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| EP0754240B1 (en) * | 1994-02-07 | 2003-08-20 | Beckman Coulter, Inc. | Ligase/polymerase-mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis |
| US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| CZ204597A3 (cs) * | 1997-06-26 | 1998-12-16 | I.N.T., Prof. Ing. Dr. Radko Krčma Drsc. | Způsob výroby objemné textilie s hladkým povrchem a zařízení k jeho provádění |
| WO1999010540A1 (en) * | 1997-08-29 | 1999-03-04 | Lopez Osvaldo J | Dna methyltransferase genotyping |
| DE19754482A1 (de) * | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| DE19935772C2 (de) * | 1999-07-26 | 2002-11-07 | Epigenomics Ag | Verfahren zur relativen Quantifizierung der Methylierung von Cytosin Basen in DNA-Proben |
| EP1942197A1 (de) * | 2000-09-01 | 2008-07-09 | Epigenomics AG | Verfahren zur Bestimmung des Methylierungsgrades von bestimmten Cytosinen in genomischer DNA im Sequenzkontext 5'-CpG-3' |
| DE10112515B4 (de) * | 2001-03-09 | 2004-02-12 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
| DE10201138B4 (de) * | 2002-01-08 | 2005-03-10 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA) |
-
1999
- 1999-10-15 DE DE19951189A patent/DE19951189C2/de not_active Expired - Fee Related
-
2000
- 2000-10-13 JP JP2001529448A patent/JP4528475B2/ja not_active Expired - Fee Related
- 2000-10-13 DE DE50010776T patent/DE50010776D1/de not_active Expired - Lifetime
- 2000-10-13 EP EP00987010A patent/EP1228246B1/de not_active Expired - Lifetime
- 2000-10-13 AU AU23451/01A patent/AU775085B2/en not_active Ceased
- 2000-10-13 NZ NZ518318A patent/NZ518318A/en not_active IP Right Cessation
- 2000-10-13 US US10/110,610 patent/US7179594B1/en not_active Expired - Fee Related
- 2000-10-13 WO PCT/DE2000/003726 patent/WO2001027317A2/de not_active Ceased
- 2000-10-13 IL IL14906700A patent/IL149067A0/xx unknown
- 2000-10-13 DK DK00987010T patent/DK1228246T3/da active
- 2000-10-13 AT AT00987010T patent/ATE299950T1/de active
- 2000-10-13 CA CA002387148A patent/CA2387148A1/en not_active Abandoned
- 2000-10-13 ES ES00987010T patent/ES2246257T3/es not_active Expired - Lifetime
-
2002
- 2002-03-27 IS IS6328A patent/IS6328A/is unknown
-
2007
- 2007-02-20 US US11/708,944 patent/US20070161036A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE19951189C2 (de) | 2003-11-06 |
| NZ518318A (en) | 2003-11-28 |
| EP1228246B1 (de) | 2005-07-20 |
| WO2001027317A3 (de) | 2002-01-17 |
| CA2387148A1 (en) | 2001-04-19 |
| DE19951189A1 (de) | 2001-05-03 |
| AU2345101A (en) | 2001-04-23 |
| AU775085B2 (en) | 2004-07-15 |
| JP4528475B2 (ja) | 2010-08-18 |
| US20070161036A1 (en) | 2007-07-12 |
| IS6328A (is) | 2002-03-27 |
| ATE299950T1 (de) | 2005-08-15 |
| IL149067A0 (en) | 2002-11-10 |
| US7179594B1 (en) | 2007-02-20 |
| DE50010776D1 (de) | 2005-08-25 |
| JP2003511056A (ja) | 2003-03-25 |
| WO2001027317A2 (de) | 2001-04-19 |
| EP1228246A2 (de) | 2002-08-07 |
| DK1228246T3 (da) | 2005-10-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100870486B1 (ko) | 게놈 디엔에이 시료의 서열 콘택스트 5'-씨피쥐-3'에서 특정 시토신의 메틸화 정도 측정방법 | |
| Laird | Principles and challenges of genome-wide DNA methylation analysis | |
| ES2688458T3 (es) | Recuento varietal de ácidos nucleicos para obtener información del número de copias genómicas | |
| JP4484431B2 (ja) | シトシンメチル化の高感度での検出方法 | |
| CA2399281C (en) | Method for detecting cytosine methylation in dna samples | |
| ES2305305T3 (es) | Procedimiento para la deteccion de una metilacion de citosina en islotes de cpg. | |
| ES2325121T3 (es) | Procedimiento para la deteccion de metilaciones de citosina en muestras de adn inmovilizado. | |
| Ai et al. | DNA methylation as a biomarker for neuropsychiatric diseases | |
| CN110484621B (zh) | 一种肝癌早期预警的方法 | |
| CN113166809B (zh) | 一种dna甲基化检测的方法、试剂盒、装置和应用 | |
| ES2599816T3 (es) | Métodos relacionados con el gen GLI3 para la detección de cáncer colorrectal | |
| US20050164193A1 (en) | Method for the analysis of methylation patterns within nucleic acids by means of mass spectrometry | |
| CN102534812A (zh) | 一种细胞dna文库及其构建方法 | |
| US20070161036A1 (en) | Method for distinguishing 5-position methylation changes of cytosine bases and cytosine-to-thymine mutations and for detecting single nucleotide polymorphisms (SNPs) or point mutations in genomic DNA | |
| JP2004529661A (ja) | 前立腺腫瘍鑑別のための方法および核酸 | |
| KR101778877B1 (ko) | 황금배와 미니배의 품종 구별을 위한 분자마커 | |
| ES2560529T3 (es) | Métodos epigenéticos | |
| ES2272636T3 (es) | Procedimiento para la determinacion cuantitativa del grado de metilac ion de citosinas en posiciones cpg. | |
| WO2022232795A1 (en) | Compositions and methods related to modification and detection of pseudouridine and 5-hydroxymethylcytosine | |
| US20050202420A1 (en) | Oligonucleotides or pna oligomers and a method for detecting the methylation state of genomic dna in a parallel manner | |
| JP2003511056A5 (https=) | ||
| US20040248120A1 (en) | Detection of specific dinucleotides in dna-samples by fluorescence resonance energy transfer (fret) | |
| CN105886497A (zh) | 多态性短串联重复基因座等位基因梯、其制备方法、鉴定及应用 | |
| US20060275806A1 (en) | Whole genome methylation profiles via single molecule analysis | |
| RU2800083C2 (ru) | Способ получения молекулярных однонуклеотидных маркеров для идентификации неизвестного индивида методом мультиплексной амплификации для работы с образцами деградированной ДНК, набор для получения молекулярных маркеров |