IL149067A0 - Method for distinguishing 5-position methylation changes - Google Patents
Method for distinguishing 5-position methylation changesInfo
- Publication number
- IL149067A0 IL149067A0 IL14906700A IL14906700A IL149067A0 IL 149067 A0 IL149067 A0 IL 149067A0 IL 14906700 A IL14906700 A IL 14906700A IL 14906700 A IL14906700 A IL 14906700A IL 149067 A0 IL149067 A0 IL 149067A0
- Authority
- IL
- Israel
- Prior art keywords
- cytosine
- methylated
- base
- way
- genomic dna
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 230000011987 methylation Effects 0.000 title abstract 2
- 238000007069 methylation reaction Methods 0.000 title abstract 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 8
- 108020004414 DNA Proteins 0.000 abstract 4
- 229940104302 cytosine Drugs 0.000 abstract 4
- 230000035772 mutation Effects 0.000 abstract 2
- 102000016397 Methyltransferase Human genes 0.000 abstract 1
- 108060004795 Methyltransferase Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- WBZKQQHYRPRKNJ-UHFFFAOYSA-L disulfite Chemical compound [O-]S(=O)S([O-])(=O)=O WBZKQQHYRPRKNJ-UHFFFAOYSA-L 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102000054765 polymorphisms of proteins Human genes 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 229940113082 thymine Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus For Radiation Diagnosis (AREA)
- Mechanical Treatment Of Semiconductor (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Stereophonic System (AREA)
- Superconductors And Manufacturing Methods Therefor (AREA)
- Paper (AREA)
- Control And Other Processes For Unpacking Of Materials (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Nonmetallic Welding Materials (AREA)
- Control Of Positive-Displacement Air Blowers (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19951189A DE19951189C2 (de) | 1999-10-15 | 1999-10-15 | Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA |
| PCT/DE2000/003726 WO2001027317A2 (de) | 1999-10-15 | 2000-10-13 | Verfahren zur unterscheidung von 5-position methylierungsänderungen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL149067A0 true IL149067A0 (en) | 2002-11-10 |
Family
ID=7926702
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL14906700A IL149067A0 (en) | 1999-10-15 | 2000-10-13 | Method for distinguishing 5-position methylation changes |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US7179594B1 (https=) |
| EP (1) | EP1228246B1 (https=) |
| JP (1) | JP4528475B2 (https=) |
| AT (1) | ATE299950T1 (https=) |
| AU (1) | AU775085B2 (https=) |
| CA (1) | CA2387148A1 (https=) |
| DE (2) | DE19951189C2 (https=) |
| DK (1) | DK1228246T3 (https=) |
| ES (1) | ES2246257T3 (https=) |
| IL (1) | IL149067A0 (https=) |
| IS (1) | IS6328A (https=) |
| NZ (1) | NZ518318A (https=) |
| WO (1) | WO2001027317A2 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19957827C2 (de) * | 1999-11-25 | 2003-06-12 | Epigenomics Ag | Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche |
| DE10010282B4 (de) * | 2000-02-25 | 2006-11-16 | Epigenomics Ag | Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| DE10151055B4 (de) * | 2001-10-05 | 2005-05-25 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln |
| DE10151069A1 (de) * | 2001-10-05 | 2003-04-30 | Epigenomics Ag | Verfahren zum Nachweis von DNA-Methylierung mittels markierten S-Adenosylmethioninanaloga |
| US6916621B2 (en) | 2002-03-27 | 2005-07-12 | Spectral Genomics, Inc. | Methods for array-based comparitive binding assays |
| US7238518B2 (en) | 2002-10-04 | 2007-07-03 | Nisshinbo Industries, Inc. | Oligonucleotide-immobilized substrate for detecting methylation |
| DE10338308B4 (de) * | 2003-08-15 | 2006-10-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in DNA |
| US20100137154A1 (en) * | 2008-12-01 | 2010-06-03 | Ach Robert A | Genome analysis using a methyltransferase |
| DE102015009187B3 (de) | 2015-07-16 | 2016-10-13 | Dimo Dietrich | Verfahren zur Bestimmung einer Mutation in genomischer DNA, Verwendung des Verfahrens und Kit zur Durchführung des Verfahrens |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| EP0754240B1 (en) * | 1994-02-07 | 2003-08-20 | Beckman Coulter, Inc. | Ligase/polymerase-mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis |
| US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| CZ204597A3 (cs) * | 1997-06-26 | 1998-12-16 | I.N.T., Prof. Ing. Dr. Radko Krčma Drsc. | Způsob výroby objemné textilie s hladkým povrchem a zařízení k jeho provádění |
| WO1999010540A1 (en) * | 1997-08-29 | 1999-03-04 | Lopez Osvaldo J | Dna methyltransferase genotyping |
| DE19754482A1 (de) * | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| DE19935772C2 (de) * | 1999-07-26 | 2002-11-07 | Epigenomics Ag | Verfahren zur relativen Quantifizierung der Methylierung von Cytosin Basen in DNA-Proben |
| EP1942197A1 (de) * | 2000-09-01 | 2008-07-09 | Epigenomics AG | Verfahren zur Bestimmung des Methylierungsgrades von bestimmten Cytosinen in genomischer DNA im Sequenzkontext 5'-CpG-3' |
| DE10112515B4 (de) * | 2001-03-09 | 2004-02-12 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
| DE10201138B4 (de) * | 2002-01-08 | 2005-03-10 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA) |
-
1999
- 1999-10-15 DE DE19951189A patent/DE19951189C2/de not_active Expired - Fee Related
-
2000
- 2000-10-13 JP JP2001529448A patent/JP4528475B2/ja not_active Expired - Fee Related
- 2000-10-13 DE DE50010776T patent/DE50010776D1/de not_active Expired - Lifetime
- 2000-10-13 EP EP00987010A patent/EP1228246B1/de not_active Expired - Lifetime
- 2000-10-13 AU AU23451/01A patent/AU775085B2/en not_active Ceased
- 2000-10-13 NZ NZ518318A patent/NZ518318A/en not_active IP Right Cessation
- 2000-10-13 US US10/110,610 patent/US7179594B1/en not_active Expired - Fee Related
- 2000-10-13 WO PCT/DE2000/003726 patent/WO2001027317A2/de not_active Ceased
- 2000-10-13 IL IL14906700A patent/IL149067A0/xx unknown
- 2000-10-13 DK DK00987010T patent/DK1228246T3/da active
- 2000-10-13 AT AT00987010T patent/ATE299950T1/de active
- 2000-10-13 CA CA002387148A patent/CA2387148A1/en not_active Abandoned
- 2000-10-13 ES ES00987010T patent/ES2246257T3/es not_active Expired - Lifetime
-
2002
- 2002-03-27 IS IS6328A patent/IS6328A/is unknown
-
2007
- 2007-02-20 US US11/708,944 patent/US20070161036A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DE19951189C2 (de) | 2003-11-06 |
| NZ518318A (en) | 2003-11-28 |
| ES2246257T3 (es) | 2006-02-16 |
| EP1228246B1 (de) | 2005-07-20 |
| WO2001027317A3 (de) | 2002-01-17 |
| CA2387148A1 (en) | 2001-04-19 |
| DE19951189A1 (de) | 2001-05-03 |
| AU2345101A (en) | 2001-04-23 |
| AU775085B2 (en) | 2004-07-15 |
| JP4528475B2 (ja) | 2010-08-18 |
| US20070161036A1 (en) | 2007-07-12 |
| IS6328A (is) | 2002-03-27 |
| ATE299950T1 (de) | 2005-08-15 |
| US7179594B1 (en) | 2007-02-20 |
| DE50010776D1 (de) | 2005-08-25 |
| JP2003511056A (ja) | 2003-03-25 |
| WO2001027317A2 (de) | 2001-04-19 |
| EP1228246A2 (de) | 2002-08-07 |
| DK1228246T3 (da) | 2005-10-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2002072880A3 (de) | Verfahren zum nachweis von cytosin-methylierungsmustern mit hoher sensitivität | |
| WO2002018632A3 (de) | Verfahren zur bestimmung des methylierungsgrades von bestimmten cytosinen in genomischer dna im sequenzkontext 5'-cpg-3' | |
| ATE506454T1 (de) | Verfahren zum nachweis von cytosin-methylierung mit hoher sensitivität | |
| DK1034309T3 (da) | Fremgangsmåde til fremstilling af komplekse DNA-methylerings-fingeraftryk | |
| CA2372665A1 (en) | Process for high throughput dna methylation analysis | |
| ATE393239T1 (de) | Verfahren zum nachweis von cytosin-methylierung in cpg-inseln | |
| WO2002086163A8 (en) | Methods for high throughput genome analysis using restriction site tagged microarrays | |
| DK0954608T3 (da) | Methyleringsspecifik påvisning | |
| IL149067A0 (en) | Method for distinguishing 5-position methylation changes | |
| AU6163399A (en) | Methods and products related to genotyping and dna analysis | |
| Nell et al. | Quantification of DNA methylation independent of sodium bisulfite conversion using methylation‐sensitive restriction enzymes and digital PCR | |
| NO20011607D0 (no) | Fremgangsmåte for analysering av polynukleotider | |
| AU2002218129A1 (en) | Method for the detection of cytosine methylations | |
| Azhikina et al. | Study of tissue-specific CpG methylation of DNA in extended genomic loci | |
| WO2004010850A3 (en) | Method for identifying cancer risk | |
| WO2001029262A3 (en) | Genotyping reagents, kits and methods of use thereof | |
| JP2003511056A5 (https=) | ||
| WO2004067775A3 (en) | Method for the detection of cytosine methylation patterns with high sensitivity | |
| WO2002004686A3 (en) | Detecting methylated cytosine in polynucleotides | |
| WO2003054224A3 (de) | Verfahren und integrierte vorrichtung zum nachweis von cytosinmethylierungen | |
| GB2414797A (en) | Quantitative multiplex detection of nucleic acids | |
| Tan et al. | A novel sequencing method for genome-wide profiling of 5-hydroxymethylcytosine with single-base resolution |