CN102534812A - 一种细胞dna文库及其构建方法 - Google Patents
一种细胞dna文库及其构建方法 Download PDFInfo
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- CN102534812A CN102534812A CN2010106196883A CN201010619688A CN102534812A CN 102534812 A CN102534812 A CN 102534812A CN 2010106196883 A CN2010106196883 A CN 2010106196883A CN 201010619688 A CN201010619688 A CN 201010619688A CN 102534812 A CN102534812 A CN 102534812A
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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CN2010106196883A CN102534812A (zh) | 2010-12-31 | 2010-12-31 | 一种细胞dna文库及其构建方法 |
PCT/CN2011/084958 WO2012089147A1 (zh) | 2010-12-31 | 2011-12-29 | 针对核酸样本构建测序文库的方法及其用途 |
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CN2010106196883A CN102534812A (zh) | 2010-12-31 | 2010-12-31 | 一种细胞dna文库及其构建方法 |
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CN102534812A true CN102534812A (zh) | 2012-07-04 |
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CN2010106196883A Pending CN102534812A (zh) | 2010-12-31 | 2010-12-31 | 一种细胞dna文库及其构建方法 |
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CN (1) | CN102534812A (zh) |
WO (1) | WO2012089147A1 (zh) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102534813A (zh) * | 2011-11-15 | 2012-07-04 | 杭州联川生物信息技术有限公司 | 构建中小片段rna测序文库的方法 |
CN102971434A (zh) * | 2010-08-11 | 2013-03-13 | 中国科学院心理研究所 | 一种甲基化dna的高通量测序方法及其应用 |
CN104152437A (zh) * | 2014-08-12 | 2014-11-19 | 上海派森诺生物科技有限公司 | 一种应用于甲基结合蛋白测序中的甲基化dna富集方法 |
CN105463090A (zh) * | 2015-12-21 | 2016-04-06 | 同济大学 | 应用于斑马鱼胚胎的先加标签的染色质免疫共沉淀高通量测序实验方法 |
CN106156536A (zh) * | 2015-04-15 | 2016-11-23 | 深圳华大基因科技有限公司 | 对样本免疫组库测序数据进行处理的方法和系统 |
CN106987645A (zh) * | 2017-05-05 | 2017-07-28 | 武汉爱基百客生物科技有限公司 | 一种表观组学技术 |
WO2017181880A1 (zh) * | 2016-04-19 | 2017-10-26 | 清华大学 | 构建待测基因组的dna测序文库的方法及其应用 |
CN107841533A (zh) * | 2017-11-13 | 2018-03-27 | 深圳先进技术研究院 | 一种染色质破碎方法及其应用 |
CN109136363A (zh) * | 2018-08-31 | 2019-01-04 | 领航基因科技(杭州)有限公司 | 一种检测胎儿染色体非整数倍的试剂盒和检测胎儿甲基化dna拷贝数的方法 |
CN109576800A (zh) * | 2018-12-07 | 2019-04-05 | 北京安智因生物技术有限公司 | 一种遗传性扩张型心肌病的基因检测文库的构建方法及其试剂盒 |
CN111386362A (zh) * | 2017-11-27 | 2020-07-07 | 深圳华大生命科学研究院 | 一种体液游离dna的文库构建方法及其应用 |
CN116218953A (zh) * | 2023-01-03 | 2023-06-06 | 南京诺唯赞生物科技股份有限公司 | 一种连接核酸片段与接头的方法 |
CN116287166A (zh) * | 2023-04-19 | 2023-06-23 | 纳昂达(南京)生物科技有限公司 | 甲基化测序接头及其应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101845500A (zh) * | 2010-05-18 | 2010-09-29 | 苏州众信生物技术有限公司 | 一种利用dna序列条码矫正二代高通量测序的序列丰度偏差的方法 |
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2010
- 2010-12-31 CN CN2010106196883A patent/CN102534812A/zh active Pending
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2011
- 2011-12-29 WO PCT/CN2011/084958 patent/WO2012089147A1/zh active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101845500A (zh) * | 2010-05-18 | 2010-09-29 | 苏州众信生物技术有限公司 | 一种利用dna序列条码矫正二代高通量测序的序列丰度偏差的方法 |
Non-Patent Citations (2)
Title |
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MARIA ROSARIA MATARAZZO等: "In vivo analysis of DNA methylation patterns recognized by specific proteins: coupling ChIP and bisulfite analysis", 《BIOTECHNIQUES》, vol. 37, no. 4, 31 October 2004 (2004-10-31) * |
MICHAEL L. SIKES 等: "A streamlined method for rapid and sensitive chromatin immunoprecipitation", 《JOURNAL OF IMMUNOLOGICAL METHODS》, vol. 344, 26 March 2009 (2009-03-26) * |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102971434A (zh) * | 2010-08-11 | 2013-03-13 | 中国科学院心理研究所 | 一种甲基化dna的高通量测序方法及其应用 |
CN102971434B (zh) * | 2010-08-11 | 2014-04-09 | 中国科学院心理研究所 | 一种甲基化dna的高通量测序方法及其应用 |
CN102534813A (zh) * | 2011-11-15 | 2012-07-04 | 杭州联川生物信息技术有限公司 | 构建中小片段rna测序文库的方法 |
CN102534813B (zh) * | 2011-11-15 | 2013-09-04 | 杭州联川生物技术有限公司 | 构建中小片段rna测序文库的方法 |
CN104152437A (zh) * | 2014-08-12 | 2014-11-19 | 上海派森诺生物科技有限公司 | 一种应用于甲基结合蛋白测序中的甲基化dna富集方法 |
CN106156536A (zh) * | 2015-04-15 | 2016-11-23 | 深圳华大基因科技有限公司 | 对样本免疫组库测序数据进行处理的方法和系统 |
CN106156536B (zh) * | 2015-04-15 | 2018-11-13 | 深圳华大基因科技有限公司 | 对样本免疫组库测序数据进行处理的方法和系统 |
CN105463090A (zh) * | 2015-12-21 | 2016-04-06 | 同济大学 | 应用于斑马鱼胚胎的先加标签的染色质免疫共沉淀高通量测序实验方法 |
WO2017181880A1 (zh) * | 2016-04-19 | 2017-10-26 | 清华大学 | 构建待测基因组的dna测序文库的方法及其应用 |
CN106987645A (zh) * | 2017-05-05 | 2017-07-28 | 武汉爱基百客生物科技有限公司 | 一种表观组学技术 |
CN107841533A (zh) * | 2017-11-13 | 2018-03-27 | 深圳先进技术研究院 | 一种染色质破碎方法及其应用 |
CN107841533B (zh) * | 2017-11-13 | 2021-11-05 | 深圳先进技术研究院 | 一种染色质破碎方法及其应用 |
CN111386362A (zh) * | 2017-11-27 | 2020-07-07 | 深圳华大生命科学研究院 | 一种体液游离dna的文库构建方法及其应用 |
CN109136363A (zh) * | 2018-08-31 | 2019-01-04 | 领航基因科技(杭州)有限公司 | 一种检测胎儿染色体非整数倍的试剂盒和检测胎儿甲基化dna拷贝数的方法 |
CN109576800A (zh) * | 2018-12-07 | 2019-04-05 | 北京安智因生物技术有限公司 | 一种遗传性扩张型心肌病的基因检测文库的构建方法及其试剂盒 |
CN116218953A (zh) * | 2023-01-03 | 2023-06-06 | 南京诺唯赞生物科技股份有限公司 | 一种连接核酸片段与接头的方法 |
CN116287166A (zh) * | 2023-04-19 | 2023-06-23 | 纳昂达(南京)生物科技有限公司 | 甲基化测序接头及其应用 |
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WO2012089147A1 (zh) | 2012-07-05 |
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