ES2217253T3 - Tioesterasas vegetales de cadena media. - Google Patents

Tioesterasas vegetales de cadena media.

Info

Publication number
ES2217253T3
ES2217253T3 ES92913220T ES92913220T ES2217253T3 ES 2217253 T3 ES2217253 T3 ES 2217253T3 ES 92913220 T ES92913220 T ES 92913220T ES 92913220 T ES92913220 T ES 92913220T ES 2217253 T3 ES2217253 T3 ES 2217253T3
Authority
ES
Spain
Prior art keywords
thioesterases
medium chain
chain vegetable
vegetable
plant thioesterases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92913220T
Other languages
English (en)
Inventor
Toni Alois Voelker
Huw Maelor Davies
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Calgene LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene LLC filed Critical Calgene LLC
Application granted granted Critical
Publication of ES2217253T3 publication Critical patent/ES2217253T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings, cooking oils
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6418Fatty acids by hydrolysis of fatty acid esters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Polymers & Plastics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

MEDIANTE ESTA INVENCION SE SUMINISTRA PROPIEDADES Y USOS ADICIONALES DE TIOESTERASAS DE CADENA MEDIA DE PLANTAS. EN UNA PRIMERA CONFORMACION, ESTA INVENCION SE REFIERE A SEMILLAS DE PLANTAS Y AL ACEITE DERIVADO DE ESAS SEMILLAS, QUE NORMALMENTE NO CONTIENE LAURATO, PERO AHORA SE HA ENCONTRADO QUE CONTIENE LAURATO. EN OTRA CONFORMACION DIFERENTE, ESTA INVENCION SE REFIERE A UNA SECUENCIA DE TIOESTERASA DE CADENA MEDIA PARTICULAR, LA SECUENCIA DE DNA DE TIOESTERASA DE CADENA MEDIA DE BAY Y A CONSTRUCCIONES DE DNA PARA LA EXPRESION DE ESTA ENZIMA EN CELULAS ANFITRIONAS. OTROS ASPECTOS DE LA INVENCION SE REFIEREN A METODOS PARA USAR UNA TIOESTERASA DE CADENA MEDIA DE PLANTA. SE SUMINISTRA UNA EXPRESION DE UNA TIOESTERASA DE CADENA MEDIA DE PLANTAS EN UNA CELULA BACTERIANA PARA PRODUCIR ACIDOS GRASOS DE CADENA MEDIA. ASIMISMO SE PRESENTAN METODOS PARA PRODUCIR UNA TIOESTERASA DE CADENA MEDIA INSATURADA MEDIANTE EL USO DE UNA TIOESTERASA DE CADENA MEDIA DE PLANTAS.
ES92913220T 1991-05-21 1992-05-21 Tioesterasas vegetales de cadena media. Expired - Lifetime ES2217253T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US70486191A 1991-05-21 1991-05-21
US77309691A 1991-10-07 1991-10-07
US78226391A 1991-10-24 1991-10-24
US07/824,247 US5512482A (en) 1990-04-26 1992-01-22 Plant thioesterases

Publications (1)

Publication Number Publication Date
ES2217253T3 true ES2217253T3 (es) 2004-11-01

Family

ID=27505488

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92913220T Expired - Lifetime ES2217253T3 (es) 1991-05-21 1992-05-21 Tioesterasas vegetales de cadena media.

Country Status (8)

Country Link
US (1) US5512482A (es)
EP (1) EP0557469B1 (es)
JP (1) JP2002238386A (es)
AT (1) ATE262042T1 (es)
CA (1) CA2109580C (es)
DE (1) DE69233323T2 (es)
ES (1) ES2217253T3 (es)
WO (1) WO1992020236A1 (es)

Families Citing this family (86)

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US7053267B2 (en) * 1990-03-16 2006-05-30 Calgene Llc Plant seed oils
US5512482A (en) * 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases
US6028247A (en) * 1990-04-26 2000-02-22 Voelker; Toni Alois Plant C18:1 preferring thioesterases
US5455167A (en) * 1991-05-21 1995-10-03 Calgene Inc. Medium-chain thioesterases in plants
GB9204583D0 (en) * 1992-03-03 1992-04-15 Unilever Plc Recombinant plant enzyme
US5654495A (en) * 1992-10-30 1997-08-05 Calgene, Inc. Production of myristate in plant cells
US5850022A (en) * 1992-10-30 1998-12-15 Calgene, Inc. Production of myristate in plant cells
WO1995006740A2 (de) * 1993-09-03 1995-03-09 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Mittelkettenspezifische thioesterasen
EP0716707A1 (de) * 1993-09-04 1996-06-19 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Promotoren
CA2176137A1 (en) * 1993-11-10 1995-05-18 Alois Toni Voelker Plant acyl acp thioesterase sequences
US5807893A (en) * 1993-11-18 1998-09-15 Voelker; Toni Alois Plant thioesterases and use for modification of fatty acid composition in plant seed oils
US6093568A (en) * 1994-04-06 2000-07-25 Calgene, Inc. Plant lysophosphatidic acid acyltransferases
US5563058A (en) * 1994-04-06 1996-10-08 Calgene, Inc. Plant lysophosphatidic acid acyltransferases
US5910630A (en) * 1994-04-06 1999-06-08 Davies; Huw Maelor Plant lysophosphatidic acid acyltransferases
DE69507467T2 (de) * 1994-05-10 1999-08-19 Unilever Nv Verfahren zur Herstellung eines Backfettes, Backfett und ein plastischer Brotaufstrich, der dieses Backfett enthält
PL319103A1 (en) * 1994-08-31 1997-07-21 Du Pont Sequences of nucleotides of palmiotolylo-acp thioesterase in colza and soya and their application in regulating content of fatty acids in oil obtained from seeds of colza and soya
JP3256952B2 (ja) 1994-11-09 2002-02-18 日本製紙株式会社 植物への遺伝子導入用ベクター、並びにこれを用いた遺伝子導入植物の作成方法及び植物への遣伝子多重導入方法
US5955329A (en) * 1995-05-15 1999-09-21 Calgene, Inc. Engineering plant thioesterases for altered substrate specificity
EP0830453A2 (en) * 1995-05-15 1998-03-25 Calgene, Inc. Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
US6150512A (en) * 1995-05-15 2000-11-21 Yuan; Ling Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
CA2205657A1 (en) * 1995-09-29 1997-04-03 Calgene, Inc. Plant stearoyl-acp thioesterase sequences and methods to increase stearate content in plant seed oils
DK0862368T3 (da) 1995-11-10 2000-04-25 Unilever Nv Spiseligt fedtsmøremateriale
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Also Published As

Publication number Publication date
DE69233323T2 (de) 2005-02-24
EP0557469B1 (en) 2004-03-17
EP0557469A4 (en) 1993-07-14
EP0557469A1 (en) 1993-09-01
WO1992020236A1 (en) 1992-11-26
CA2109580A1 (en) 1992-11-26
US5512482A (en) 1996-04-30
DE69233323D1 (de) 2004-04-22
JP2002238386A (ja) 2002-08-27
CA2109580C (en) 2009-08-04
ATE262042T1 (de) 2004-04-15

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