ES2180092T3 - Inmunoensayo marcado con una enzima y dispositivo para ello. - Google Patents

Inmunoensayo marcado con una enzima y dispositivo para ello.

Info

Publication number
ES2180092T3
ES2180092T3 ES98107362T ES98107362T ES2180092T3 ES 2180092 T3 ES2180092 T3 ES 2180092T3 ES 98107362 T ES98107362 T ES 98107362T ES 98107362 T ES98107362 T ES 98107362T ES 2180092 T3 ES2180092 T3 ES 2180092T3
Authority
ES
Spain
Prior art keywords
enzyme
labeled reagent
labeled
zone
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98107362T
Other languages
English (en)
Inventor
Yoshihiro Ashihara
Mitsuo Isomura
Atsuka Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Inc
Original Assignee
Fujirebio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujirebio Inc filed Critical Fujirebio Inc
Application granted granted Critical
Publication of ES2180092T3 publication Critical patent/ES2180092T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/81—Tube, bottle, or dipstick

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE LLEVA A CABO UN INMUNOENSAYO DE IDENTIFICACION DE ENZIMAS MEDIANTE LAS ETAPAS DE PERMITIR QUE UNA MUESTRA DE ENSAYO REACCIONE CON UN REACTIVO DE IDENTIFICACION DE ENCIMAS, PERMITIENDO QUE UN SUSTRATO REACCIONE CON LA ENZIMA PARA FORMAR UN INDICADOR, E INMOVILIZAR EL REACTIVO, EVITANDO LA FORMACION POSTERIOR DE INDICADOR TRANSCURRIDO UN TIEMPO DETERMINADO TRAS LA INMOVILIZACION DEL REACTIVO IDENTIFICADOR DE ENZIMAS, UTILIZANDO UN INHIBIDOR DE ENZIMAS. EL DISPOSITIVO PARA REALIZAR ESTE INMUNOENSAYO DE IDENTIFICACION DE ENZIMAS INCLUYE UN MATERIAL ABSORBENTE CAPAZ DE TRANSPORTAR UNA SOLUCION DE REVELADO POR ACCION CAPILAR, INCLUYENDO EL MATERIAL ABSORBENTE (A) UNA ZONA DE APLICACION DE LIQUIDO DE REVELADO, (B) UNA ZONA DE RECTIVO IDENTIFICADOR DE ENZIMAS QUE CONTIENE UN RECTIVO IDENTIFICADOR DE ENZIMAS, (C) UNA ZONA DE ALOJAMIENTO DE MUESTRA Y (D) UNA ZONA DE REACTIVO INDICADOR CAPAZ DE INMOVILIZAR EL REACTIVO IDENTIFICADOR DE ENZIMAS TRAS LA REACCION DE LA MUESTRA DE ENSAYO CON EL REACTIVOIDENTIFICADOR DE ENZIMAS EN UNA CANTIDAD DEPENDIENTE DEL RESULTADO DEL ENSAYO, SIENDO APLICADO UN INHIBIDOR DE ENZIMAS A UNA PARTE DEL MATERIAL ABSORBENTE AGUAS ARRIBA DE LA ZONA DE REACTIVO IDENTIFICADOR DE ENZIMAS, EVITANDO EL INHIBIDOR DE ENZIMAS LA FORMACION DE UN INDICADOR UNA VEZ TRANSCURRIDO UN TIEMPO PREDETERMINADO TRAS SER INMOVILIZADO EL RECTIVO IDENTIFICADOR DE ENZIMAS EN LA ZONA DE REACTIVO INDICADOR.
ES98107362T 1997-04-23 1998-04-22 Inmunoensayo marcado con una enzima y dispositivo para ello. Expired - Lifetime ES2180092T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11864697A JP3334558B2 (ja) 1997-04-23 1997-04-23 酵素免疫測定方法及び試験片

Publications (1)

Publication Number Publication Date
ES2180092T3 true ES2180092T3 (es) 2003-02-01

Family

ID=14741717

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98107362T Expired - Lifetime ES2180092T3 (es) 1997-04-23 1998-04-22 Inmunoensayo marcado con una enzima y dispositivo para ello.

Country Status (6)

Country Link
US (2) US6221625B1 (es)
EP (1) EP0874240B1 (es)
JP (1) JP3334558B2 (es)
AT (1) ATE221659T1 (es)
DE (1) DE69806841T2 (es)
ES (1) ES2180092T3 (es)

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AT501069A1 (de) * 2001-08-20 2006-06-15 Walter Ing Pils Vorrichtung und verfahren zum nachweis eines analyten
WO2003056333A1 (fr) * 2001-12-27 2003-07-10 Matsushita Electric Industrial Co., Ltd. Methode de dosage immunologique et trousse de reactif de dosage immunologique destinee a etre utilisee dans ladite methode
JP5392980B2 (ja) * 2003-03-27 2014-01-22 チルドレンズ ホスピタル メディカル センター 早発型の腎尿細管細胞障害を検出するための方法およびキット
KR20060054333A (ko) * 2003-07-23 2006-05-22 후지레비오 가부시끼가이샤 항-인플루엔자 ｂ형 바이러스 모노클로날 항체 및 상기항체를 사용하는 면역 측정 기구
CN1934133B (zh) * 2003-07-23 2011-12-14 富士瑞必欧株式会社 抗a型流感病毒单克隆抗体及使用该抗体的免疫测定器具
JPWO2005042579A1 (ja) * 2003-10-31 2008-01-10 富士レビオ株式会社 抗ｓａｒｓウイルス抗体、該抗体を産生するハイブリドーマ及び該抗体を用いる免疫測定試薬
US20050272101A1 (en) * 2004-06-07 2005-12-08 Prasad Devarajan Method for the early detection of renal injury
US7473678B2 (en) 2004-10-14 2009-01-06 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods of use thereof
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WO2006098804A2 (en) 2005-03-11 2006-09-21 Chembio Diagnostic Systems, Inc. Dual path immunoassay device
US20070037232A1 (en) * 2005-03-31 2007-02-15 Barasch Jonathan M Detection of NGAL in chronic renal disease
US7850923B2 (en) * 2005-05-04 2010-12-14 Bionostics, Inc. Container for maintaining stabilized control solution and container for single-use control solution including prior use indicator
US20060263244A1 (en) * 2005-05-04 2006-11-23 Rannikko Minna A Devices, systems, and methods for the containment and use of liquid solutions
US7749453B2 (en) * 2005-05-04 2010-07-06 Bionostics, Inc. Devices, systems, and methods for the containment and use of liquid solutions
US20070040501A1 (en) 2005-08-18 2007-02-22 Aitken Bruce G Method for inhibiting oxygen and moisture degradation of a device and the resulting device
US7722929B2 (en) * 2005-08-18 2010-05-25 Corning Incorporated Sealing technique for decreasing the time it takes to hermetically seal a device and the resulting hermetically sealed device
US7829147B2 (en) * 2005-08-18 2010-11-09 Corning Incorporated Hermetically sealing a device without a heat treating step and the resulting hermetically sealed device
US20080206589A1 (en) * 2007-02-28 2008-08-28 Bruce Gardiner Aitken Low tempertature sintering using Sn2+ containing inorganic materials to hermetically seal a device
US20080090304A1 (en) * 2006-10-13 2008-04-17 Barasch Jonathan Matthew Diagnosis and monitoring of chronic renal disease using ngal
EP1951327A2 (en) * 2005-11-17 2008-08-06 Biomimetic Therapeutics, Inc. Maxillofacial bone augmentation using rhpdgf-bb and a biocompatible matrix
WO2007092622A2 (en) * 2006-02-09 2007-08-16 Biomimetic Therapeutics, Inc. Compositions and methods for treating bone
US9161967B2 (en) * 2006-06-30 2015-10-20 Biomimetic Therapeutics, Llc Compositions and methods for treating the vertebral column
JP5484047B2 (ja) 2006-06-30 2014-05-07 バイオミメティック セラピューティクス， エルエルシー 回旋筋腱板傷害を処置するためのｐｄｇｆ−生体マトリックス組成物および方法
US20080048178A1 (en) * 2006-08-24 2008-02-28 Bruce Gardiner Aitken Tin phosphate barrier film, method, and apparatus
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BRPI0918611B8 (pt) 2008-09-09 2021-06-22 Biomimetic Therapeutics Inc composição que compreende uma matriz biocompatível e um fator de crescimento derivado de plaqueta e kit
JP2010117213A (ja) * 2008-11-12 2010-05-27 Dic Plastics Inc 酵素免疫測定器具及び酵素免疫測定方法
WO2010102266A1 (en) * 2009-03-05 2010-09-10 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods for the treatment of osteochondral defects
CN107080834A (zh) 2010-02-22 2017-08-22 生物模拟治疗有限责任公司 用于治疗腱病的血小板衍生生长因子组合物和方法
WO2011111108A1 (ja) * 2010-03-11 2011-09-15 Ｄｉｃプラスチック株式会社 酵素免疫測定器具及び酵素免疫測定方法
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Also Published As

Publication number Publication date
ATE221659T1 (de) 2002-08-15
DE69806841D1 (de) 2002-09-05
US20010005585A1 (en) 2001-06-28
JPH10300750A (ja) 1998-11-13
DE69806841T2 (de) 2003-03-13
EP0874240A1 (en) 1998-10-28
US6221625B1 (en) 2001-04-24
EP0874240B1 (en) 2002-07-31
JP3334558B2 (ja) 2002-10-15

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