ES2180092T3 - Inmunoensayo marcado con una enzima y dispositivo para ello. - Google Patents

Inmunoensayo marcado con una enzima y dispositivo para ello.

Info

Publication number
ES2180092T3
ES2180092T3 ES98107362T ES98107362T ES2180092T3 ES 2180092 T3 ES2180092 T3 ES 2180092T3 ES 98107362 T ES98107362 T ES 98107362T ES 98107362 T ES98107362 T ES 98107362T ES 2180092 T3 ES2180092 T3 ES 2180092T3
Authority
ES
Spain
Prior art keywords
enzyme
labeled reagent
labeled
zone
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98107362T
Other languages
English (en)
Inventor
Yoshihiro Ashihara
Mitsuo Isomura
Atsuka Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Inc
Original Assignee
Fujirebio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujirebio Inc filed Critical Fujirebio Inc
Application granted granted Critical
Publication of ES2180092T3 publication Critical patent/ES2180092T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/81—Tube, bottle, or dipstick

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE LLEVA A CABO UN INMUNOENSAYO DE IDENTIFICACION DE ENZIMAS MEDIANTE LAS ETAPAS DE PERMITIR QUE UNA MUESTRA DE ENSAYO REACCIONE CON UN REACTIVO DE IDENTIFICACION DE ENCIMAS, PERMITIENDO QUE UN SUSTRATO REACCIONE CON LA ENZIMA PARA FORMAR UN INDICADOR, E INMOVILIZAR EL REACTIVO, EVITANDO LA FORMACION POSTERIOR DE INDICADOR TRANSCURRIDO UN TIEMPO DETERMINADO TRAS LA INMOVILIZACION DEL REACTIVO IDENTIFICADOR DE ENZIMAS, UTILIZANDO UN INHIBIDOR DE ENZIMAS. EL DISPOSITIVO PARA REALIZAR ESTE INMUNOENSAYO DE IDENTIFICACION DE ENZIMAS INCLUYE UN MATERIAL ABSORBENTE CAPAZ DE TRANSPORTAR UNA SOLUCION DE REVELADO POR ACCION CAPILAR, INCLUYENDO EL MATERIAL ABSORBENTE (A) UNA ZONA DE APLICACION DE LIQUIDO DE REVELADO, (B) UNA ZONA DE RECTIVO IDENTIFICADOR DE ENZIMAS QUE CONTIENE UN RECTIVO IDENTIFICADOR DE ENZIMAS, (C) UNA ZONA DE ALOJAMIENTO DE MUESTRA Y (D) UNA ZONA DE REACTIVO INDICADOR CAPAZ DE INMOVILIZAR EL REACTIVO IDENTIFICADOR DE ENZIMAS TRAS LA REACCION DE LA MUESTRA DE ENSAYO CON EL REACTIVOIDENTIFICADOR DE ENZIMAS EN UNA CANTIDAD DEPENDIENTE DEL RESULTADO DEL ENSAYO, SIENDO APLICADO UN INHIBIDOR DE ENZIMAS A UNA PARTE DEL MATERIAL ABSORBENTE AGUAS ARRIBA DE LA ZONA DE REACTIVO IDENTIFICADOR DE ENZIMAS, EVITANDO EL INHIBIDOR DE ENZIMAS LA FORMACION DE UN INDICADOR UNA VEZ TRANSCURRIDO UN TIEMPO PREDETERMINADO TRAS SER INMOVILIZADO EL RECTIVO IDENTIFICADOR DE ENZIMAS EN LA ZONA DE REACTIVO INDICADOR.
ES98107362T 1997-04-23 1998-04-22 Inmunoensayo marcado con una enzima y dispositivo para ello. Expired - Lifetime ES2180092T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11864697A JP3334558B2 (ja) 1997-04-23 1997-04-23 酵素免疫測定方法及び試験片

Publications (1)

Publication Number Publication Date
ES2180092T3 true ES2180092T3 (es) 2003-02-01

Family

ID=14741717

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98107362T Expired - Lifetime ES2180092T3 (es) 1997-04-23 1998-04-22 Inmunoensayo marcado con una enzima y dispositivo para ello.

Country Status (6)

Country Link
US (2) US6221625B1 (es)
EP (1) EP0874240B1 (es)
JP (1) JP3334558B2 (es)
AT (1) ATE221659T1 (es)
DE (1) DE69806841T2 (es)
ES (1) ES2180092T3 (es)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6627459B1 (en) * 2000-04-19 2003-09-30 Applied Biotech, Inc. Immunoassay controls
MXPA03006284A (es) * 2001-01-15 2003-09-16 Dsm Ip Assets Bv Dispositivo de ensayo.
GB0109925D0 (en) * 2001-04-23 2001-06-13 Axis Shield Asa Method
AT501069A1 (de) * 2001-08-20 2006-06-15 Walter Ing Pils Vorrichtung und verfahren zum nachweis eines analyten
EP1369689B1 (en) * 2001-12-27 2008-03-26 Matsushita Electric Industrial Co., Ltd. Immunoassay method and immunoassay reagent kit to be used therein
AU2004225472B2 (en) * 2003-03-27 2011-02-10 Children's Hospital Medical Center A method and kit for detecting the early onset of renal tubular cell injury
CN1934133B (zh) * 2003-07-23 2011-12-14 富士瑞必欧株式会社 抗a型流感病毒单克隆抗体及使用该抗体的免疫测定器具
CN100441597C (zh) * 2003-07-23 2008-12-10 富士瑞必欧株式会社 抗b型流感病毒单克隆抗体及使用该抗体的免疫测定器具
US20080254440A1 (en) * 2003-10-31 2008-10-16 Yoshiaki Uchida Anti-Sars Virus Antibody, Hybridoma Producing the Antibody and Immunoassay Reagent Using the Antibody
US20050272101A1 (en) * 2004-06-07 2005-12-08 Prasad Devarajan Method for the early detection of renal injury
US7473678B2 (en) 2004-10-14 2009-01-06 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods of use thereof
US7442557B1 (en) 2004-10-22 2008-10-28 Idexx Laboratories, Inc. Bi-directional flow assay device with reagent bridge
US7189522B2 (en) 2005-03-11 2007-03-13 Chembio Diagnostic Systems, Inc. Dual path immunoassay device
WO2006098804A2 (en) 2005-03-11 2006-09-21 Chembio Diagnostic Systems, Inc. Dual path immunoassay device
US20070037232A1 (en) * 2005-03-31 2007-02-15 Barasch Jonathan M Detection of NGAL in chronic renal disease
US20060263244A1 (en) * 2005-05-04 2006-11-23 Rannikko Minna A Devices, systems, and methods for the containment and use of liquid solutions
US7749453B2 (en) * 2005-05-04 2010-07-06 Bionostics, Inc. Devices, systems, and methods for the containment and use of liquid solutions
US7850923B2 (en) * 2005-05-04 2010-12-14 Bionostics, Inc. Container for maintaining stabilized control solution and container for single-use control solution including prior use indicator
US20080206589A1 (en) * 2007-02-28 2008-08-28 Bruce Gardiner Aitken Low tempertature sintering using Sn2+ containing inorganic materials to hermetically seal a device
US7722929B2 (en) 2005-08-18 2010-05-25 Corning Incorporated Sealing technique for decreasing the time it takes to hermetically seal a device and the resulting hermetically sealed device
US20070040501A1 (en) * 2005-08-18 2007-02-22 Aitken Bruce G Method for inhibiting oxygen and moisture degradation of a device and the resulting device
US7829147B2 (en) 2005-08-18 2010-11-09 Corning Incorporated Hermetically sealing a device without a heat treating step and the resulting hermetically sealed device
US20080090304A1 (en) * 2006-10-13 2008-04-17 Barasch Jonathan Matthew Diagnosis and monitoring of chronic renal disease using ngal
CA2630077C (en) 2005-11-17 2014-07-15 Biomimetic Therapeutics, Inc. Maxillofacial bone augmentation using rhpdgf-bb and a biocompatible matrix
WO2007092622A2 (en) 2006-02-09 2007-08-16 Biomimetic Therapeutics, Inc. Compositions and methods for treating bone
US9161967B2 (en) 2006-06-30 2015-10-20 Biomimetic Therapeutics, Llc Compositions and methods for treating the vertebral column
ES2664229T3 (es) * 2006-06-30 2018-04-18 Biomimetic Therapeutics, Llc Composiciones y métodos de biomatriz-PDGF para el tratamiento de lesiones del manguito rotador
US20080048178A1 (en) * 2006-08-24 2008-02-28 Bruce Gardiner Aitken Tin phosphate barrier film, method, and apparatus
EP2086598B1 (en) 2006-11-03 2015-05-27 BioMimetic Therapeutics, LLC Compositions and methods for arthrodetic procedures
US20080195476A1 (en) * 2007-02-09 2008-08-14 Marchese Michael A Abandonment remarketing system
ES2422259T3 (es) * 2008-02-07 2013-09-10 Biomimetic Therapeutics Inc Composiciones para la osteogénesis por distracción
US20090263905A1 (en) 2008-04-18 2009-10-22 Kim Scheuringer Detection test assembly for detecting the presence of a substance in a sample
US20100022916A1 (en) 2008-07-24 2010-01-28 Javanbakhsh Esfandiari Method and Apparatus for Collecting and Preparing Biological Samples for Testing
NZ591338A (en) * 2008-09-09 2013-02-22 Biomimetic Therapeutics Inc Platelet-derived growth factor compositions and methods for the treatment of tendon and ligament injuries
JP2010117213A (ja) * 2008-11-12 2010-05-27 Dic Plastics Inc 酵素免疫測定器具及び酵素免疫測定方法
US20100247651A1 (en) * 2009-03-05 2010-09-30 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods for the treatment of osteochondral defects
WO2011103598A1 (en) 2010-02-22 2011-08-25 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods for the treatment of tendinopathies
WO2011111108A1 (ja) * 2010-03-11 2011-09-15 Ｄｉｃプラスチック株式会社 酵素免疫測定器具及び酵素免疫測定方法
US8603835B2 (en) 2011-02-10 2013-12-10 Chembio Diagnostic Systems, Inc. Reduced step dual path immunoassay device and method
US20140004547A1 (en) * 2011-03-23 2014-01-02 Anp Technologies Inc. Rapid Tests for the Detection of Inhibitors of Enzymes and Human Exposure to the Same
US20130108670A1 (en) 2011-10-25 2013-05-02 Biomimetic Therapeutics, Inc. Compositions and methods for treating full thickness burn injuries
WO2013062995A1 (en) 2011-10-25 2013-05-02 Biomimetic Therapeutics, Inc. Compositions and methods for treating deep partial and full thickness wounds and injuries
US9791376B2 (en) * 2012-06-12 2017-10-17 Panasonic Intellectual Property Management Co., Ltd. Optical sensor
WO2014116727A1 (en) * 2013-01-23 2014-07-31 Anp Technologies, Inc. One-step rapid assay for the detection of inhibitors of enzymes
MX2016012881A (es) 2014-04-02 2017-05-10 Chembio Diagnostic Systems Inc Inmunoensayo que utiliza un conjugado de captura.
JP6871855B2 (ja) 2014-10-14 2021-05-19 サムエル リンチ， 創傷を処置するための組成物
US20160116466A1 (en) 2014-10-27 2016-04-28 Chembio Diagnostic Systems, Inc. Rapid Screening Assay for Qualitative Detection of Multiple Febrile Illnesses
BR112019019630A2 (pt) 2017-05-19 2020-04-14 Philip Morris Products Sa teste diagnóstico para distinguir o status de tabagismo de um sujeito
JP7241696B2 (ja) 2017-12-22 2023-03-17 株式会社三和化学研究所 イムノクロマトグラフィー装置

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4585792A (en) * 1983-05-05 1986-04-29 Technology Unlimited Inc. Protective additive to vaginal products and catamenials
US4835099A (en) 1986-11-20 1989-05-30 Becton, Dickinson And Company Signal enhancement in immunoassay by modulation of enzymatic catalysis
EP0312565B1 (en) 1987-04-29 1993-03-03 Celltech Limited Binding assay device
US4962024A (en) 1988-08-11 1990-10-09 Becton, Dickinson And Company Signal enhancement in assay for an enzyme
US5296347A (en) * 1991-02-08 1994-03-22 Ciba Corning Diagnostics Corp. Bridge immunoassay
TW213503B (es) 1991-05-02 1993-09-21 Fujirebio Kk

Also Published As

Publication number Publication date
DE69806841D1 (de) 2002-09-05
US6221625B1 (en) 2001-04-24
ATE221659T1 (de) 2002-08-15
JP3334558B2 (ja) 2002-10-15
EP0874240B1 (en) 2002-07-31
DE69806841T2 (de) 2003-03-13
US20010005585A1 (en) 2001-06-28
EP0874240A1 (en) 1998-10-28
JPH10300750A (ja) 1998-11-13

Similar Documents

Publication Publication Date Title
ES2180092T3 (es) Inmunoensayo marcado con una enzima y dispositivo para ello.
KR890005513A (ko) 측유동 크로마토그래피 결합분석장치
DE3878820T2 (de) Vorrichtung fuer bindungstest.
DE69521104D1 (de) Spezifische Bindungstests und Reagenzien dafür
DK165198C (da) Analysesaet, testplade eller -ark omfattende en flerhed af analysesaet, samt en bindingsanalyse som udfoeres under anvendelse af et analysesaet
ATE226729T1 (de) Quantitative immunochromatographische assays
ES2079434T3 (es) Ensayos de ligandos-receptores que utilizan un umbral.
DE69624920D1 (de) Testreagentien und Testvorrichtungen
DK165932C (da) Anordning og fremgangsmaade til hurtig kvalitativ og kvantitativ bestemmelse af tilstedevaerelsen af en reaktiv ligand i et fluidum
DK0728309T3 (da) Analyseindretninger og fremstilling heraf
FI875210A (fi) Foerfarande och testanordning foer paovisning av ett aemne i ett prov.
JP4113956B2 (ja) 水晶振動子を用いた微量物質の測定方法
ATE67597T1 (de) Verfahren und materialien zur durchfuehrung von spezifischen bindungstests.
DK0638806T3 (da) Immunoassaymetode
JPH08166388A (ja) 酵素免疫測定方法及び酵素免疫センサ
ES2056132T3 (es) Agente para ensayos inmunoquimicos que contiene oxidos de amina.
DK1200826T3 (da) Immunoassay og testanordning med integreret reference
KR930700849A (ko) 면역진단 검정법