ES2175416T3 - Enmascaramiento de la fluorescencia y luminiscencia de fondo en el analisis optico de ensayos biomedicos. - Google Patents

Enmascaramiento de la fluorescencia y luminiscencia de fondo en el analisis optico de ensayos biomedicos.

Info

Publication number
ES2175416T3
ES2175416T3 ES97927032T ES97927032T ES2175416T3 ES 2175416 T3 ES2175416 T3 ES 2175416T3 ES 97927032 T ES97927032 T ES 97927032T ES 97927032 T ES97927032 T ES 97927032T ES 2175416 T3 ES2175416 T3 ES 2175416T3
Authority
ES
Spain
Prior art keywords
solution
light
lighting
mask
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97927032T
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English (en)
Inventor
Thomas Krahn
Wolfgang Paffhausen
Andreas Schade
Martin Bechem
Delf Schmidt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
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Bayer AG
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=7795451&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2175416(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bayer AG filed Critical Bayer AG
Application granted granted Critical
Publication of ES2175416T3 publication Critical patent/ES2175416T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/823—Immunogenic carrier or carrier per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/13—Tracers or tags

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

EN UN PROCEDIMIENTO PARA EL ANALISIS OPTICO CUANTITATIVO DE CELULAS BIOLOGICAS (5) MARCADAS CON FLUORESCENCIA, SE APLICA UNA CAPA CELULAR EN UN SOPORTE TRANSPARENTE EN LA BASE (2) DE UN RECIPIENTE DE REACCION (1), DE TAL MANERA QUE ESTA EN CONTACTO CON LA SOLUCION (3) QUE CONTIENE EL TINTE FLUORESCENTE (4). LA SENSIBILIDAD DE LA DETERMINACION SE PUEDE MEJORAR DE MANERA SIGNIFICANTE AÑADIENDO A LA SOLUCION (3) UN TINTE DE ENMASCARAMIENTO (9), QUE ABSORBE LA LUZ DE EXCITACION (6) DEL TINTE FLUORESCENTE (4) QUE SE ENCUENTRA PRESENTE EN LA SOLUCION (3) Y/O SU LUZ EMITIDA (7) Y/O APLICANDO UNA CAPA INTERMEDIA (10) QUE ES PERMEABLE A LA SOLUCION PERO ABSORBE Y/O REFLEJA LA LUZ DE EXCITACION (6) O LA LUZ EMITIDA (7) A LA CAPA CELULAR DE LA BASE (2). SE PUEDE UTILIZAR EL MISMO PROCEDIMIENTO PARA MEJORAR LA SENSIBILIDAD EN ANALISIS OPTICOS CUANTITATIVOS DE UNA CAPA CELULAR BIOLUMINISCENTE. EN ESTE ULTIMO CASO LA CAPA INTERMEDIA (10) DEBERIA ESTAR DE TAL MANERA COMPUESTA QUE TUVIERA UN ELEVADO FACTORDE REFLEXION CON RESPECTO A LA LUZ LUMINISCENTE (11). DE MANERA ANALOGA, ESTOS PRINCIPIOS SE PUEDEN APLICAR A ESTUDIOS DEL RECEPTOR PARA ENMASCARAR RADIACIONES DE ALTERACION DE FONDO EN ANALISIS OPTICOS Y CUANTITATIVOS DE COMPONENTES MARCADOS POR LUMINISCENCIA O FLUORESCENCIA EN UNA REACCION. EN ESTE CASO UNA CAPA RECEPTORA (12) SE COLOCA EN LA BASE (2) DE UN RECIPIENTE DE REACCION (1) EN CONTACTO CON UNA SOLUCION (SOBRENADANTE 3) EN LA CUAL SE HA DISUELTO UN LIGANDO FLUORESCENTE O LUMINISCENTE (13). LA SENSIBILIDAD Y REPRODUCTIVIDAD DE LA DETERMINACION SE PUEDEN MEJORAR DE MANERA SIGNIFICANTE SI SE AÑADE UN TINTE DE ENMASCARAMIENTO (9) QUE ABSORBE LA LUZ DE EXCITACION (6) PARA EL TINTE FLUORESCENTE Y/O SU LUZ EMITIDA O (CASO DE LIGANDOS LUMINISCENTES) LA LUZ LUMINISCENTE, ESTO SE AÑADE AL SOBRENADANTE (3). UNA CAPA INTERMEDIA (10) QUE ES PERMEABLE A LA SOLUCION (3) PERO ABSORBE Y/O REFLEJA LA LUZ DE EXCITACION (6) Y/O LA LUZ EMITIDA O LA LUZ LUMINISCENTE SE PUEDE APLICAR A LA CAPA CELULARO RECEPTOR (12) EN LA BASE (2) EN VEZ DEL TINTE DE ENMASCARAMIENTO EN LA SOLUCION (3) O SI ES POSIBLE COMO UNA MEDIDA SUPLEMENTARIA.
ES97927032T 1996-05-28 1997-05-23 Enmascaramiento de la fluorescencia y luminiscencia de fondo en el analisis optico de ensayos biomedicos. Expired - Lifetime ES2175416T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19621312A DE19621312A1 (de) 1996-05-28 1996-05-28 Maskierung der Hintergrundfluoreszenz und Signalverstärkung bei der optischen Analyse biologisch medizinischer Assays

Publications (1)

Publication Number Publication Date
ES2175416T3 true ES2175416T3 (es) 2002-11-16

Family

ID=7795451

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97927032T Expired - Lifetime ES2175416T3 (es) 1996-05-28 1997-05-23 Enmascaramiento de la fluorescencia y luminiscencia de fondo en el analisis optico de ensayos biomedicos.

Country Status (9)

Country Link
US (5) US6420183B1 (es)
EP (1) EP0906572B1 (es)
JP (1) JP3452068B2 (es)
AT (1) ATE215698T1 (es)
CA (1) CA2256629C (es)
DE (2) DE19621312A1 (es)
DK (1) DK0906572T3 (es)
ES (1) ES2175416T3 (es)
WO (1) WO1997045739A1 (es)

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Also Published As

Publication number Publication date
WO1997045739A1 (de) 1997-12-04
DE19621312A1 (de) 1997-12-04
US7615376B2 (en) 2009-11-10
US20020009754A1 (en) 2002-01-24
US20030092081A1 (en) 2003-05-15
US20020015969A1 (en) 2002-02-07
ATE215698T1 (de) 2002-04-15
CA2256629A1 (en) 1997-12-04
CA2256629C (en) 2003-07-22
DE59706875D1 (de) 2002-05-08
JP2000512746A (ja) 2000-09-26
DK0906572T3 (da) 2002-07-08
US20080318270A1 (en) 2008-12-25
US6420183B1 (en) 2002-07-16
US7138280B2 (en) 2006-11-21
US20020022274A1 (en) 2002-02-21
JP3452068B2 (ja) 2003-09-29
EP0906572B1 (de) 2002-04-03
US8178359B2 (en) 2012-05-15
EP0906572A1 (de) 1999-04-07
US7063952B2 (en) 2006-06-20

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