ES2170860T3 - Procedimiento de ensayo de extension de dna polimerasa para endonucleasa del virus de la gripe. - Google Patents

Procedimiento de ensayo de extension de dna polimerasa para endonucleasa del virus de la gripe.

Info

Publication number
ES2170860T3
ES2170860T3 ES96920610T ES96920610T ES2170860T3 ES 2170860 T3 ES2170860 T3 ES 2170860T3 ES 96920610 T ES96920610 T ES 96920610T ES 96920610 T ES96920610 T ES 96920610T ES 2170860 T3 ES2170860 T3 ES 2170860T3
Authority
ES
Spain
Prior art keywords
dna polymerase
endonuclease
assay
flu
virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96920610T
Other languages
English (en)
Inventor
James L Cole
Lawrence C Kuo
David B Olsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Application granted granted Critical
Publication of ES2170860T3 publication Critical patent/ES2170860T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE HA DESARROLLADO UN ENSAYO DE LA ENDONUCLEASA DEL VIRUS DE LA GRIPE QUE CONSISTE EN UNA EXTENSION CATALIZADA POR UNA POLIMERASA DEL DNA DEL PRODUCTO DE EXFOLIACION DE LA ENDONUCLEASA VIRAL QUE HACE USO DE NUCLEOTIDOS ETIQUETADOS Y DE UN MOLDE DE DNA QUE CONTIENE UNA REGION 3 COMPLEMENTARIA AL PRODUCTO UNIDA A UNA REGION 5 QUE CONSISTE EN RESIDUOS REPETIDOS. EL ENSAYO ACOPLADO A LA POLIMERASA DEL DNA NO INCLUYE UNA SEPARACION ELECTROFORETICA DE GEL Y PERMITE SELECCIONAR UN ALTO VOLUMEN DE INHIBIDORES POTENCIALES. OTRA CARACTERISTICA CLAVE DEL ENSAYO ES QUE ES LO SUFICIENTEMENTE SENSIBLE COMO PARA DETECTAR 200 ATTOMOLES DE PRODUCTO.
ES96920610T 1995-06-07 1996-06-03 Procedimiento de ensayo de extension de dna polimerasa para endonucleasa del virus de la gripe. Expired - Lifetime ES2170860T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/487,759 US5660989A (en) 1995-06-07 1995-06-07 DNA polymerase extension assay for influenza virus endonuclease

Publications (1)

Publication Number Publication Date
ES2170860T3 true ES2170860T3 (es) 2002-08-16

Family

ID=23937008

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96920610T Expired - Lifetime ES2170860T3 (es) 1995-06-07 1996-06-03 Procedimiento de ensayo de extension de dna polimerasa para endonucleasa del virus de la gripe.

Country Status (8)

Country Link
US (1) US5660989A (es)
EP (1) EP0832292B1 (es)
JP (1) JPH11506606A (es)
AT (1) ATE212675T1 (es)
CA (1) CA2222753A1 (es)
DE (1) DE69618955T2 (es)
ES (1) ES2170860T3 (es)
WO (1) WO1996040993A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2170861T3 (es) * 1995-06-07 2002-08-16 Merck & Co Inc Procedimiento de ensayo de extension de adn polimerasa.
US6100028A (en) * 1996-06-03 2000-08-08 Merck & Co., Inc. DNA polymerase extension assay
MXPA03002995A (es) 2000-10-05 2004-12-06 Hong Kong Dna Chips Ltd Equipo para detectar virus subtipo h5 de influenza a, patogenos y no patogenos.
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
WO2014164874A2 (en) 2013-03-13 2014-10-09 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
ES2170861T3 (es) * 1995-06-07 2002-08-16 Merck & Co Inc Procedimiento de ensayo de extension de adn polimerasa.

Also Published As

Publication number Publication date
CA2222753A1 (en) 1996-12-19
EP0832292A1 (en) 1998-04-01
ATE212675T1 (de) 2002-02-15
JPH11506606A (ja) 1999-06-15
EP0832292B1 (en) 2002-01-30
DE69618955D1 (de) 2002-03-14
US5660989A (en) 1997-08-26
DE69618955T2 (de) 2002-08-14
WO1996040993A1 (en) 1996-12-19
EP0832292A4 (en) 1999-05-26

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