DE69618955D1 - Verfahren zur detektion von influenzavirus endonuklease durch dna-polymerase verlängerung - Google Patents

Verfahren zur detektion von influenzavirus endonuklease durch dna-polymerase verlängerung

Info

Publication number
DE69618955D1
DE69618955D1 DE69618955T DE69618955T DE69618955D1 DE 69618955 D1 DE69618955 D1 DE 69618955D1 DE 69618955 T DE69618955 T DE 69618955T DE 69618955 T DE69618955 T DE 69618955T DE 69618955 D1 DE69618955 D1 DE 69618955D1
Authority
DE
Germany
Prior art keywords
dna polymerase
assay
product
polymerase extension
virus endonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69618955T
Other languages
English (en)
Other versions
DE69618955T2 (de
Inventor
L Cole
C Kuo
B Olsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Publication of DE69618955D1 publication Critical patent/DE69618955D1/de
Application granted granted Critical
Publication of DE69618955T2 publication Critical patent/DE69618955T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69618955T 1995-06-07 1996-06-03 Verfahren zur detektion von influenzavirus endonuklease durch dna-polymerase verlängerung Expired - Fee Related DE69618955T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/487,759 US5660989A (en) 1995-06-07 1995-06-07 DNA polymerase extension assay for influenza virus endonuclease
PCT/US1996/008320 WO1996040993A1 (en) 1995-06-07 1996-06-03 Dna polymerase extension assay for influenza virus endonuclease

Publications (2)

Publication Number Publication Date
DE69618955D1 true DE69618955D1 (de) 2002-03-14
DE69618955T2 DE69618955T2 (de) 2002-08-14

Family

ID=23937008

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69618955T Expired - Fee Related DE69618955T2 (de) 1995-06-07 1996-06-03 Verfahren zur detektion von influenzavirus endonuklease durch dna-polymerase verlängerung

Country Status (8)

Country Link
US (1) US5660989A (de)
EP (1) EP0832292B1 (de)
JP (1) JPH11506606A (de)
AT (1) ATE212675T1 (de)
CA (1) CA2222753A1 (de)
DE (1) DE69618955T2 (de)
ES (1) ES2170860T3 (de)
WO (1) WO1996040993A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0833945B1 (de) * 1995-06-07 2002-01-30 Merck & Co., Inc. Dna-polymerase verlängungs assay
US6100028A (en) * 1996-06-03 2000-08-08 Merck & Co., Inc. DNA polymerase extension assay
ATE435305T1 (de) 2000-10-05 2009-07-15 Hai Kang Life Corp Ltd Kit zum nachweis von nichtpathogenem oder pathogenem influenza-a-virus vom subtyp h5
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
WO2014164874A2 (en) 2013-03-13 2014-10-09 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
EP0833945B1 (de) * 1995-06-07 2002-01-30 Merck & Co., Inc. Dna-polymerase verlängungs assay

Also Published As

Publication number Publication date
JPH11506606A (ja) 1999-06-15
EP0832292A4 (de) 1999-05-26
WO1996040993A1 (en) 1996-12-19
ES2170860T3 (es) 2002-08-16
DE69618955T2 (de) 2002-08-14
EP0832292A1 (de) 1998-04-01
US5660989A (en) 1997-08-26
EP0832292B1 (de) 2002-01-30
ATE212675T1 (de) 2002-02-15
CA2222753A1 (en) 1996-12-19

Similar Documents

Publication Publication Date Title
DE69838839D1 (de) Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen
DE3675684D1 (de) Diagnostisches reagenz, satz und verfahren unter verwendung von polynukleotid-versetzung, trennung, enzymatischer spaltung und nachweis von adenosinphosphat.
ATE201238T1 (de) Dns-sequenzierung durch stufenweise ligierung und spaltung
DE68926784D1 (de) Verfahren zur charakterisierung von hla dp
DE69814629D1 (de) Verfahren und verbindungen zur analysierung von nukleinsaeuren durch massenspektrometrie
DE69628626D1 (de) Verfahren zur Reinigung und Übertragung von sequenzierten DNA-Proben nach Trenn-/Feststellungssystem und Platte dafür
DE69434117D1 (de) Verfahren zur typisierung von hepatitis c viren und dafür zu verwendende reagenzien
ATE257246T1 (de) Verfahren zur analytischen trennung von viren
ATE188744T1 (de) Verfahren zur erhöhung der nachweisempfindlichkeit von nukleinsäure-hybriden (target/probe)
DE69524083D1 (de) Stoffen und verfahren zum nachweis von mycobakterium tuberculosis
DE69627408D1 (de) Primers und Sonden zum Nachweis von HIV
DE69607830D1 (de) Verfahren zur isothermer amplifikation von nukleinsäure molekülen
ATE236269T1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
ATE278771T1 (de) Vorrichtung und verfahren zur analyse flüssiger proben
DE69624824D1 (de) Verfahren zur erkennung, identifizierung, isolierung, selektiver markierung und zielgerichteter erkennung von th1 lymphozyten mit hilfe von lag-3 protein
DE69518697D1 (de) Nachweis von Treponema Pallidum und Haemophilus Ducreyi
DE69618955D1 (de) Verfahren zur detektion von influenzavirus endonuklease durch dna-polymerase verlängerung
SE9702008D0 (sv) A method and a system for nucleic acid seouence analysis
ATE130047T1 (de) Charakterisierung von human-leukocyt-antigen.
DE69940890D1 (de) Ein verfahren zur analyse von polynukleotiden
ATE264546T1 (de) Verfahren und vorrichtung zum erkennen der katalytischen aktivität von feststoffen
DE69330604D1 (de) Verfahren und system zur molekularbiologischen diagnose
DE60311244D1 (de) Verfahren und automatische extraktion von nucleinsäuren aus komplexen mischungen
DE69618957D1 (de) Dna-polymerase verlängungs assay
ATE359369T1 (de) Rns-abhängige rns-polymerase, die vorzugsweise anhand einer rna-matrix funktioniert und ein promoter-abhängiger transkriptionsprozess , der diese rns-abhängige rns-polymerase benutzt.

Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee