DE60311244D1 - Verfahren und automatische extraktion von nucleinsäuren aus komplexen mischungen - Google Patents

Verfahren und automatische extraktion von nucleinsäuren aus komplexen mischungen

Info

Publication number
DE60311244D1
DE60311244D1 DE60311244T DE60311244T DE60311244D1 DE 60311244 D1 DE60311244 D1 DE 60311244D1 DE 60311244 T DE60311244 T DE 60311244T DE 60311244 T DE60311244 T DE 60311244T DE 60311244 D1 DE60311244 D1 DE 60311244D1
Authority
DE
Germany
Prior art keywords
nucleic acids
microorganisms
complex mixtures
automatic extraction
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60311244T
Other languages
English (en)
Other versions
DE60311244T2 (de
Inventor
Sylvie Hallier-Soulier
Gaille Leborgne
Nadine Batisse-Debitte
Gabriel Festoc
Xavier Badiche
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pall Genedisc Technologies SAS
Original Assignee
GeneSystems SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GeneSystems SA filed Critical GeneSystems SA
Application granted granted Critical
Publication of DE60311244D1 publication Critical patent/DE60311244D1/de
Publication of DE60311244T2 publication Critical patent/DE60311244T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE60311244T 2002-04-09 2003-04-09 Verfahren und automatische extraktion von nucleinsäuren aus komplexen mischungen Expired - Lifetime DE60311244T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0204424 2002-04-09
FR0204424A FR2838066B1 (fr) 2002-04-09 2002-04-09 Procede et automate d'extraction d'acides nucleiques a partir d'un melange complexe
PCT/FR2003/001123 WO2003085109A2 (fr) 2002-04-09 2003-04-09 Procede et automate d'extraction d'acides nucleiques a partir d'un melange complexe

Publications (2)

Publication Number Publication Date
DE60311244D1 true DE60311244D1 (de) 2007-03-08
DE60311244T2 DE60311244T2 (de) 2007-11-15

Family

ID=28052243

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60311244T Expired - Lifetime DE60311244T2 (de) 2002-04-09 2003-04-09 Verfahren und automatische extraktion von nucleinsäuren aus komplexen mischungen

Country Status (10)

Country Link
EP (1) EP1495119B1 (de)
JP (1) JP2005526509A (de)
AT (1) ATE351903T1 (de)
AU (1) AU2003246804A1 (de)
DE (1) DE60311244T2 (de)
DK (1) DK1495119T3 (de)
ES (1) ES2279960T3 (de)
FR (1) FR2838066B1 (de)
PT (1) PT1495119E (de)
WO (1) WO2003085109A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2829500B1 (fr) 2001-09-13 2003-12-12 Hemosystem Procede de concentration et de detection de germes pathogenes a partir de produits sanguins et/ou de leurs derives et dispositif pour le mettre en oeuvre
US20050250138A1 (en) 2004-05-04 2005-11-10 Millipore Corporation Process for the enumeration and identification of microorganisms
JP4195039B2 (ja) * 2006-02-16 2008-12-10 善之 辻本 検体の前処理方法および試薬
FR2901281B1 (fr) * 2006-05-19 2008-08-15 Hemosystem Sa Procede d'extraction des acides desoxyribonucleiques (adn)de microorganismes eventuellement presents dans un echantillon sanguin
JP2008005781A (ja) * 2006-06-29 2008-01-17 Olympus Corp 試料処理方法
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
WO2010111265A1 (en) 2009-03-24 2010-09-30 University Of Chicago Slip chip device and methods
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
KR102458821B1 (ko) * 2015-12-29 2022-10-25 국민대학교산학협력단 필터를 이용한 미생물 신속 검출 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3788082T2 (de) * 1986-04-11 1994-06-09 Applied Biosystems Automatisches Gerät zum Extrahieren von Nukleinsäuren.
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
FR2678950B1 (fr) * 1991-07-09 1993-11-05 Bertin Et Cie Cartouche, dispositif et procede d'extraction d'acides nucleiques tels que l'adn a partir d'un echantillon de sang ou de cellules de tissus.
AU6104994A (en) * 1993-03-18 1994-10-11 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture Apparatus for the quantitative determination of particulate analytes
FR2712511B1 (fr) * 1993-11-16 1996-02-09 Bertin & Cie Cartouche pour la préparation d'acides nucléiques purifiés.
AU1752799A (en) * 1997-08-27 1999-04-05 Anda Biologicals S.A. Rapid filter diagnostic device and method for the direct detection of micro-organisms in body fluids
US20020010323A1 (en) * 1998-10-09 2002-01-24 Andrew Martin Mitchell Isolation method and apparatus
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products

Also Published As

Publication number Publication date
ES2279960T3 (es) 2007-09-01
JP2005526509A (ja) 2005-09-08
DE60311244T2 (de) 2007-11-15
DK1495119T3 (da) 2007-05-21
WO2003085109A2 (fr) 2003-10-16
ATE351903T1 (de) 2007-02-15
WO2003085109A3 (fr) 2004-04-01
EP1495119A2 (de) 2005-01-12
AU2003246804A1 (en) 2003-10-20
FR2838066A1 (fr) 2003-10-10
PT1495119E (pt) 2007-04-30
FR2838066B1 (fr) 2004-06-25
EP1495119B1 (de) 2007-01-17

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