ATE48431T1 - Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger. - Google Patents

Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger.

Info

Publication number
ATE48431T1
ATE48431T1 AT86101883T AT86101883T ATE48431T1 AT E48431 T1 ATE48431 T1 AT E48431T1 AT 86101883 T AT86101883 T AT 86101883T AT 86101883 T AT86101883 T AT 86101883T AT E48431 T1 ATE48431 T1 AT E48431T1
Authority
AT
Austria
Prior art keywords
solid support
nucleoside
phosphate
oligonucleotide
rapid
Prior art date
Application number
AT86101883T
Other languages
English (en)
Inventor
Nanibhushan Dattagupta
Original Assignee
Molecular Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Diagnostics Inc filed Critical Molecular Diagnostics Inc
Application granted granted Critical
Publication of ATE48431T1 publication Critical patent/ATE48431T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AT86101883T 1985-02-22 1986-02-14 Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger. ATE48431T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/704,129 US4818681A (en) 1985-02-22 1985-02-22 Fast and specific immobilization of nucleic acids to solid supports
EP86101883A EP0192197B1 (de) 1985-02-22 1986-02-14 Schnelle und spezifische Immobilisierung von Nukleinsäuren am festen Träger

Publications (1)

Publication Number Publication Date
ATE48431T1 true ATE48431T1 (de) 1989-12-15

Family

ID=24828201

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86101883T ATE48431T1 (de) 1985-02-22 1986-02-14 Schnelle und spezifische immobilisierung von nukleinsaeuren am festen traeger.

Country Status (5)

Country Link
US (1) US4818681A (de)
EP (1) EP0192197B1 (de)
JP (1) JPS61246201A (de)
AT (1) ATE48431T1 (de)
DE (1) DE3667291D1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948882A (en) * 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
US5714380A (en) 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
JPH084519B2 (ja) * 1987-12-25 1996-01-24 エフ・ホフマン―ラ ロシュ アーゲー ハイブリッド形成用担体およびその調整方法
US5352578A (en) * 1989-02-15 1994-10-04 Worcester Foundation For Experimental Biology Method of separating oligonucleotides from a mixture
US5559221A (en) * 1989-02-15 1996-09-24 Worcester Foundation For Experimental Biology Method of separating oligonucleotides from a mixture
WO1991000868A1 (en) * 1989-07-07 1991-01-24 National Research Development Corporation Immobilised polynucleotides
US5079169A (en) * 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments
US5770358A (en) * 1991-09-18 1998-06-23 Affymax Technologies N.V. Tagged synthetic oligomer libraries
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
TW323284B (de) * 1992-03-23 1997-12-21 Novartis Ag
GB9207380D0 (en) * 1992-04-03 1992-05-13 Ici Plc Compounds
US5382512A (en) * 1993-08-23 1995-01-17 Chiron Corporation Assay device with captured particle reagent
US5503805A (en) * 1993-11-02 1996-04-02 Affymax Technologies N.V. Apparatus and method for parallel coupling reactions
US6165778A (en) * 1993-11-02 2000-12-26 Affymax Technologies N.V. Reaction vessel agitation apparatus
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
JP2001524808A (ja) 1996-12-10 2001-12-04 ジーントレイス・システムズ・インコーポレイテッド 放出可能な不揮発性の質量標識分子
US5905024A (en) * 1996-12-17 1999-05-18 University Of Chicago Method for performing site-specific affinity fractionation for use in DNA sequencing
US6979728B2 (en) * 1998-05-04 2005-12-27 Baylor College Of Medicine Articles of manufacture and methods for array based analysis of biological molecules
US6048695A (en) 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
JP3996307B2 (ja) * 1999-11-05 2007-10-24 富士フイルム株式会社 Ｄｎａ断片の固定方法、ｄｎａチップおよび核酸断片の検出方法
AU2001233043A1 (en) * 2000-01-28 2001-08-07 Linden Technologies, Inc. C-3' protected monomeric nucleotides and synthesis of oligonucleotides on solid support
EP1356088A2 (de) * 2000-06-07 2003-10-29 Baylor College of Medicine Zusammensetzungen und methoden zur array-gestützten nukleinsäurehybridisierung
US6861214B1 (en) * 2000-10-23 2005-03-01 Beckman Coulter, Inc. Immobilization of biopolymers to aminated substrates by direct adsorption
US20050221304A1 (en) * 2001-04-11 2005-10-06 Charlie Xiang Modified random primers for probe labeling
US20030170675A1 (en) * 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
WO2003091426A1 (en) * 2001-10-12 2003-11-06 Spectral Genomics, Inc. Compilations of nucleic acids and arrays and methods of using them
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
US20030124599A1 (en) * 2001-11-14 2003-07-03 Shiping Chen Biochemical analysis system with combinatorial chemistry applications
US20030124542A1 (en) * 2001-12-28 2003-07-03 Spectral Genomics, Inc. Methods for mapping the chromosomal loci of genes expressed by a cell
US20060141465A1 (en) * 2002-12-02 2006-06-29 Toyo Kohan Co. Ltd. Method of immobilizing a polynucleotide on a solid support, solid immobilization support thus obtained, method of detecting target polynucleotide from the solid support and solution for immobilizing the polynucleotide
EP2961386B1 (de) 2013-02-28 2019-07-10 The General Hospital Corporation Mirna-profilierungszusammensetzungen und verfahren zur verwendung
FI3846824T3 (fi) 2018-09-07 2025-09-25 Massachusetts Gen Hospital Koostumuksia ja menetelmiä immuunitarkistuspisteen estämiseen

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1083508A (fr) * 1975-11-13 1980-08-12 Jacques Grange Supports porteurs de chaines laterales, procedes d'obtention de ces supports, procedes de fixation de composes organiques comportant un residu glucidique sur lesdits supports, produits et reactifs resultant de ladite fixation chimique
DE2841414C2 (de) * 1978-09-22 1980-04-03 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig Verfahren zur Herstellung von an Makromoleküle gebundenen, ein Adeninringsystem enthaltenden Koenzymen
JPS5564599A (en) * 1978-11-08 1980-05-15 Unitika Ltd Adenosine triphosphate derivative
CA1168229A (en) * 1980-02-29 1984-05-29 University Patents, Inc. Supports for use in synthesis of polynucleotides and process for making and using the same
US4401796A (en) * 1981-04-30 1983-08-30 City Of Hope Research Institute Solid-phase synthesis of polynucleotides
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn
US4472572A (en) * 1982-02-01 1984-09-18 Innovax Laboratories, Ltd. Adenosine pyrophosphate polynucleotide compound
US4474948A (en) * 1982-04-08 1984-10-02 Biosearch Benzazolides and their employment in phosphite ester oligonucleotide synthesis processes
US4474947A (en) * 1982-04-08 1984-10-02 Biosearch Benzazolides and their employment in phosphate ester oligonucleotide synthesis processes
US4591564A (en) * 1982-11-10 1986-05-27 The University Of Montana Transferase enzymes which modify the 3'-termini of ribonucleic acid and methods
EP0135587B2 (de) * 1983-02-22 2002-12-18 Syngene, Inc. Einzelstrang-oligonukleotide bestimmter sequenz, markergruppen enthaltend, verfahren zu deren chemischer synthese und nukleotide zur verwendung darin
JP2548112B2 (ja) * 1983-09-02 1996-10-30 シンジェン，インコーポレイテッド 担体およびオリゴヌクレオチドの合成
EP0177497A4 (de) * 1984-04-05 1987-07-06 Life Technologies Inc Immobilisierung von nukleinsäuren.
FR2567523B1 (fr) * 1984-07-12 1987-11-13 Pasteur Institut Polynucleotides lies de facon covalente a un support solide, et procede pour leur obtention
JPS61112093A (ja) * 1984-11-05 1986-05-30 Wakunaga Seiyaku Kk ヌクレオチド誘導体

Also Published As

Publication number Publication date
EP0192197A2 (de) 1986-08-27
EP0192197B1 (de) 1989-12-06
JPS61246201A (ja) 1986-11-01
EP0192197A3 (en) 1987-12-23
DE3667291D1 (de) 1990-01-11
US4818681A (en) 1989-04-04

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee