ES2170861T3 - Procedimiento de ensayo de extension de adn polimerasa. - Google Patents
Procedimiento de ensayo de extension de adn polimerasa.Info
- Publication number
- ES2170861T3 ES2170861T3 ES96920611T ES96920611T ES2170861T3 ES 2170861 T3 ES2170861 T3 ES 2170861T3 ES 96920611 T ES96920611 T ES 96920611T ES 96920611 T ES96920611 T ES 96920611T ES 2170861 T3 ES2170861 T3 ES 2170861T3
- Authority
- ES
- Spain
- Prior art keywords
- product
- assay
- oligonucleotide
- dna polymerase
- test procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
SE HA DESARROLLADO UN ANALISIS DE ENZIMAS QUE ACTUAN SOBRE SUSTRATOS PARA PRODUCIR UN PRODUCTO OLIGONUCLEOTIDO DE CADENA SENCILLA QUE IMPLICA LA EXTENSION DEL ADN CATALIZADA POR POLIMERASA DEL PRODUCTO DE DIVISION DEL OLIGONUCLEOTIDO UTILIZANDO NUCLEOTIDOS MARCADOS Y UNA PLANTILLA TEMPLETE DE ADN QUE CONTIENE UNA REGION 3'' COMPLEMENTARIA DEL PRODUCTO OLIGONUCLEOTIDO UNIDO A LA REGION 5'' FORMADA POR RESIDUOS REPETIDOS DE OLIGONUCLEOTIDOS. EL ANALISIS DE EXTENSION DE LA ADN POLIMERASA NO CONLLEVA SEPARACION ELECTROFORETICA EN GEL Y ES SENSIBLE PARA LA DETECCION SELECTIVA DE GRANDES VOLUMENES DE INHIBIDORES POTENCIALES. OTRAS CARACTERISTICAS FUNDAMENTALES DEL ENSAYO SON QUE CONTROLA LA REACCION DE DIVISION DEL SUSTRATO SOLO EN LA POSICION CORRECTA DE LA SECUENCIA, CON LO QUE DISCRIMINA ENTRE PRODUCTOS DE DIVISION INESPECIFICOS, Y QUE TIENE UNA SENSIBILIDAD SUFICIENTE PARA DETECTAR 200 ATOMOLES DE PRODUCTO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US48776095A | 1995-06-07 | 1995-06-07 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2170861T3 true ES2170861T3 (es) | 2002-08-16 |
Family
ID=23937013
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96920611T Expired - Lifetime ES2170861T3 (es) | 1995-06-07 | 1996-06-03 | Procedimiento de ensayo de extension de adn polimerasa. |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0833945B1 (es) |
JP (1) | JPH11506607A (es) |
AT (1) | ATE212676T1 (es) |
CA (1) | CA2222688A1 (es) |
DE (1) | DE69618957T2 (es) |
ES (1) | ES2170861T3 (es) |
WO (1) | WO1996040994A1 (es) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5660989A (en) * | 1995-06-07 | 1997-08-26 | Merck & Co., Inc. | DNA polymerase extension assay for influenza virus endonuclease |
IL122147A0 (en) * | 1997-11-10 | 1998-04-05 | Technion Res & Dev Foundation | Method for labeling polynucleotides |
IL138126A0 (en) * | 1998-03-05 | 2001-10-31 | Johnson & Johnson Res Pty Ltd | Zymogenic nucleic acid detection methods and related molecules and kits |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4521509A (en) * | 1982-11-24 | 1985-06-04 | Research Corporation | Method for degrading DNA |
US4994368A (en) * | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
US5660989A (en) * | 1995-06-07 | 1997-08-26 | Merck & Co., Inc. | DNA polymerase extension assay for influenza virus endonuclease |
-
1996
- 1996-06-03 EP EP96920611A patent/EP0833945B1/en not_active Expired - Lifetime
- 1996-06-03 AT AT96920611T patent/ATE212676T1/de active
- 1996-06-03 CA CA002222688A patent/CA2222688A1/en not_active Abandoned
- 1996-06-03 DE DE69618957T patent/DE69618957T2/de not_active Expired - Fee Related
- 1996-06-03 ES ES96920611T patent/ES2170861T3/es not_active Expired - Lifetime
- 1996-06-03 JP JP9500964A patent/JPH11506607A/ja not_active Ceased
- 1996-06-03 WO PCT/US1996/008330 patent/WO1996040994A1/en active Search and Examination
Also Published As
Publication number | Publication date |
---|---|
EP0833945A4 (en) | 1999-05-19 |
ATE212676T1 (de) | 2002-02-15 |
DE69618957T2 (de) | 2002-08-14 |
DE69618957D1 (de) | 2002-03-14 |
WO1996040994A1 (en) | 1996-12-19 |
EP0833945A1 (en) | 1998-04-08 |
CA2222688A1 (en) | 1996-12-19 |
JPH11506607A (ja) | 1999-06-15 |
EP0833945B1 (en) | 2002-01-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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