ES2121698A1 - Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna. - Google Patents

Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.

Info

Publication number
ES2121698A1
ES2121698A1 ES09602551A ES9602551A ES2121698A1 ES 2121698 A1 ES2121698 A1 ES 2121698A1 ES 09602551 A ES09602551 A ES 09602551A ES 9602551 A ES9602551 A ES 9602551A ES 2121698 A1 ES2121698 A1 ES 2121698A1
Authority
ES
Spain
Prior art keywords
viral
sub
amplified fragments
pcr
tube
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09602551A
Other languages
English (en)
Other versions
ES2121698B1 (es
Inventor
Urgoiti Begona Blanco
Ascaso Fernando Ponz
Pascual Vicente Torres
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Original Assignee
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA filed Critical Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria INIA
Priority to ES09602551A priority Critical patent/ES2121698B1/es
Priority to PCT/ES1998/000128 priority patent/WO1999058725A1/es
Publication of ES2121698A1 publication Critical patent/ES2121698A1/es
Application granted granted Critical
Publication of ES2121698B1 publication Critical patent/ES2121698B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE FRAGMENTOS AMPLIFICADOS DE ACIDO NUCLEICO DE PARTICULAS VIRALES Y SUBVIRALES QUE CONTIENEN RNA. EL PROCEDIMIENTO COMPRENDE CAPTURAR SOBRE UN TUBO DE UN TERMOCICLADOR LAS PARTICULAS VIRALES O SUBVIRALES, AÑADIR AL TUBO LOS REACTIVOS NECESARIOS PARA EFECTUAR LA TRANSCRIPCION INVERSA (TR) Y LA AMPLIFICACION ENZIMATICA POR REACCION EN CADENA DE LA POLIMERASA (PCR), INTRODUCIR EL TUBO EN EL TERMOCICLADOR Y APLICAR UN CICLO TERMICO ADECUADO PARA EFECTUAR PRIMERO LA TR Y POSTERIORMENTE LA PCR SIN NECESIDAD DE AÑADIR REACTIVOS ENTRE AMBAS REACCIONES NI MANIPULAR LOS TUBOS CON MUESTRAS, PARA LO CUAL, EN LA PCR SE UTILIZA UNA DNA POLIMERASA TERMOESTABLE. LOS FRAGMENTOS AMPLIFICADOS PUEDEN SER SOMETIDOS A DIVERSOS ANALISIS PARA DETECTAR, IDENTIFICAR Y TIPIFICAR LA PARTICULA VIRAL O SUBVIRAL. ESTE PROCEDIMIENTO TIENE APLICACION EN DIAGNOSTICO Y ESTUDIOS DE EPIDEMIOLOGIA MOLECULAR.
ES09602551A 1996-12-02 1996-12-02 Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna. Expired - Fee Related ES2121698B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES09602551A ES2121698B1 (es) 1996-12-02 1996-12-02 Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
PCT/ES1998/000128 WO1999058725A1 (es) 1996-12-02 1998-05-08 Procedimiento para la obtencion de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES09602551A ES2121698B1 (es) 1996-12-02 1996-12-02 Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
PCT/ES1998/000128 WO1999058725A1 (es) 1996-12-02 1998-05-08 Procedimiento para la obtencion de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna

Publications (2)

Publication Number Publication Date
ES2121698A1 true ES2121698A1 (es) 1998-12-01
ES2121698B1 ES2121698B1 (es) 1999-07-01

Family

ID=26155008

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09602551A Expired - Fee Related ES2121698B1 (es) 1996-12-02 1996-12-02 Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.

Country Status (2)

Country Link
ES (1) ES2121698B1 (es)
WO (1) WO1999058725A1 (es)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0479158A1 (en) * 1990-09-29 1992-04-08 Shimadzu Corporation Reagent for RNA detection and RNA detection method using it
US5290677A (en) * 1990-01-24 1994-03-01 The United States Of America As Represented By The Department Of Health And Human Services Rapid and sensitive test for detecting hepatitis A virus
EP0632134A2 (en) * 1993-07-01 1995-01-04 F. Hoffmann-La Roche Ag Reagents and methods for coupled high temperature reverse transcription and polymerase chain reaction

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009944A2 (en) * 1989-12-22 1991-07-11 F.Hoffmann-La Roche Ag High temperature reverse transcriptases
US5077192A (en) * 1988-10-25 1991-12-31 The General Hospital Corporation Method of detecting antigenic, nucleic acid-containing macromolecular entities
IE64040B1 (en) * 1989-06-15 1995-06-28 Akzo Nv Method for determining nucleic acid
ES2044784B1 (es) * 1992-06-12 1994-09-01 Inia Procedimiento para la deteccion e identificacion de patogenos virales y subvirales.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290677A (en) * 1990-01-24 1994-03-01 The United States Of America As Represented By The Department Of Health And Human Services Rapid and sensitive test for detecting hepatitis A virus
EP0479158A1 (en) * 1990-09-29 1992-04-08 Shimadzu Corporation Reagent for RNA detection and RNA detection method using it
EP0632134A2 (en) * 1993-07-01 1995-01-04 F. Hoffmann-La Roche Ag Reagents and methods for coupled high temperature reverse transcription and polymerase chain reaction

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NOLASCO, G. et al., "A method combining immunicapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral phatogens", JOURNAL OF VIROLOGICAL METHODS, 1993, Vol. 45, pßgs. 201-218. Ver el documento completo *

Also Published As

Publication number Publication date
ES2121698B1 (es) 1999-07-01
WO1999058725A1 (es) 1999-11-18

Similar Documents

Publication Publication Date Title
AU685903B2 (en) Detection of nucleic acid amplification
CA2076793A1 (en) Methods and reagents for hepatitis c detection
WO1999063112A3 (en) Pcr techniques for detecting microbial and viral contaminants in foodstuffs
WO2001098528A3 (de) Verfahren zum nachweis von cytosin-methylierungen
EP0656068A1 (en) Amplification and detection process
CA2191182A1 (en) Oligonucleotide primers for amplifying hcv nucleic acid
NZ506333A (en) An assay for detecting and quantifying the presence of particular nucleic acid (DNA or RNA) sequences
WO2003020891A3 (en) Single nucleotide amplification and detection by polymerase
KR930700680A (ko) Rna 레플리카제의 dna-의존성 rna 폴리머라제 활성을 이용한 핵산 증폭
EP1350841A3 (en) Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity
EP2324128A1 (en) Temperature controlled nucleic-acid detection method suitable for practice in a closed-system
CA2127188A1 (en) Reagents and Methods for Coupled High Temperature Reverse Transcription and Polymerase Chain Reactions
DE60016275D1 (en) Helicobacter pylori antigene in blut
KR20210130612A (ko) 핵산을 단순하게 추출하고 분석하여 표적핵산 검사 방법 및 장치
CN107523624B (zh) 一种结合audg和自避分子识别系统的多交叉恒温扩增方法
WO2001075139A8 (en) Reversible chemical modification of nucleic acids and improved method for nucleic acid hybridization
JPH0811070B2 (ja) 核酸増幅反応の汚染を防除する方法
US20080233587A1 (en) Method for direct amplification from crude nucleic acid samples
WO2003012142A1 (en) Detection of nucleic acids by real-time pcr using chimeric rna-dna primers
WO2004013305A3 (en) Improved compositions for in vitro amplification of nucleic acids
ES2121698A1 (es) Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
US20050123934A1 (en) Novel method for DNA amplification using DNA blocking probes
AU5026196A (en) Methods and reagents for typing hla class i genes
CN109457013B (zh) 交叉引物扩增结合纳米生物传感器和audg检测核酸的方法
CA3064500C (en) Multiplex nucleic acid amplification assay

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 19981201

Kind code of ref document: A1

Effective date: 19981201

FD1A Patent lapsed

Effective date: 20071203