ES2170751T3 - Promotor aox2 mutante, vector portador del mismo, transformante, y produccion de proteina heterologa. - Google Patents
Promotor aox2 mutante, vector portador del mismo, transformante, y produccion de proteina heterologa.Info
- Publication number
- ES2170751T3 ES2170751T3 ES93117527T ES93117527T ES2170751T3 ES 2170751 T3 ES2170751 T3 ES 2170751T3 ES 93117527 T ES93117527 T ES 93117527T ES 93117527 T ES93117527 T ES 93117527T ES 2170751 T3 ES2170751 T3 ES 2170751T3
- Authority
- ES
- Spain
- Prior art keywords
- promoter
- aox2
- protein
- transformant
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
LA APLICACION COMPRENDE UN PROMOTOR AOX2 MUTANTE OBTENIDO POR MUTACION DE UNA SECUENCIA DEL PROMOTOR AOX2 NATURAL DE MANERA A COMPRENDER AL MENOS UNO DE LOS TRES MODOS DE (1) UNA REGION QUE SE EXTIENDE CORRIENTE ARRIBA DESDE EL NUCLEOTIDO 1187 INCLUSIVO Y QUE COMPRENDE AL MENOS NUCLEOTIDOS 845-960 SE SUPRIME (2) (A) NUCLEOTIDO(S) SE SUSTITUYE(N) EN (A) REGION(ES) DE NUCLEOTIDOS 1274-1314 Y (3) SE INSERTAN NUEVO(S) NUCLEOTIDA(S) EN (A) REGION(ES) DE NUCLEOTIDOS 1274-1314; VECTORES QUE LLEVAN DICHO PROMOTOR AOX2 MUTANTE; SE HAN INTRODUCIDO TRANSFORMANTES DENTRO DE DICHO VECTOR ; Y METODOS PARA PRODUCIR UNA PROTEINA HETEROLOGA QUE COMPRENDEN EL CULTIVO DE DICHO TRANSFORMANTE. EL PROMOTOR DE LA PRESENTE INVENCION TIENE UNA ACTIVIDAD NOTABLEMENTE REALZADA AL COMPARARSE CON EL PROMOTOR AOX2 NATURAL Y ES ALTAMENTE UTIL COMO PROMOTOR A SER LLEVADO EN UN VECTOR DE EXPRESION QUE PERMITE LA EXPRESION DE PROTEINA HETEROLOGA ADEMAS. EL VECTOR Y EL TRANSFORMANTE DE LA INVENCION PUEDEN EXPRESARSE EFICIENTEMENTE Y PRODUCIR PROTEINAS HETEROLOGAS UTILES.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP29331592 | 1992-10-30 | ||
JP5215306A JPH06189769A (ja) | 1992-10-30 | 1993-08-06 | 変異型aox2プロモーター、それを担持するベクター、形質転換体および異種蛋白質の製造方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2170751T3 true ES2170751T3 (es) | 2002-08-16 |
Family
ID=26520803
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES93117527T Expired - Lifetime ES2170751T3 (es) | 1992-10-30 | 1993-10-28 | Promotor aox2 mutante, vector portador del mismo, transformante, y produccion de proteina heterologa. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5683893A (es) |
EP (1) | EP0595334B1 (es) |
JP (1) | JPH06189769A (es) |
KR (1) | KR100393297B1 (es) |
CA (1) | CA2102043C (es) |
DE (1) | DE69331434T2 (es) |
DK (1) | DK0595334T3 (es) |
ES (1) | ES2170751T3 (es) |
TW (1) | TW343997B (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3505743B2 (ja) * | 1993-07-27 | 2004-03-15 | 三菱ウェルファーマ株式会社 | 変異型aox2プロモーター、それを担持するベクター、形質転換体および異種蛋白質の製造方法 |
US6183953B1 (en) | 1997-09-15 | 2001-02-06 | Zymogenetics, Inc. | Chromosomal mutagenesis in Pichia methanolica |
AU2002213398A1 (en) * | 2000-10-20 | 2002-05-06 | Whitehead Institute For Biomedical Research | Expression vectors and uses thereof |
MXPA03009059A (es) * | 2001-04-05 | 2004-10-15 | Univ Nebraska | Secuencias de nucleotidos reguladoras de alcohol oxidasa 1 para expresion de genes heterologos en levadura. |
CN1308452C (zh) * | 2004-09-14 | 2007-04-04 | 上海永业农科生物工程有限公司 | 一种含有改组乙醇氧化酶启动子的毕赤酵母植酸酶表达系统 |
US20070142288A1 (en) | 2005-12-20 | 2007-06-21 | Pappachan Kolattukudy | Isolated MCPIP and methods of use |
CN112831501A (zh) * | 2016-05-04 | 2021-05-25 | 深圳普罗吉医药科技有限公司 | 高效表达重组人血清白蛋白工程菌的构建 |
CN114959919A (zh) * | 2021-02-23 | 2022-08-30 | 中国科学院天津工业生物技术研究所 | 一种构建酿酒酵母人工小启动子文库的方法及应用 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2063890C (en) * | 1991-03-27 | 2007-03-13 | Masami Miura | Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism |
-
1993
- 1993-08-06 JP JP5215306A patent/JPH06189769A/ja active Pending
- 1993-10-08 TW TW082108332A patent/TW343997B/zh not_active IP Right Cessation
- 1993-10-28 ES ES93117527T patent/ES2170751T3/es not_active Expired - Lifetime
- 1993-10-28 DK DK93117527T patent/DK0595334T3/da active
- 1993-10-28 EP EP93117527A patent/EP0595334B1/en not_active Expired - Lifetime
- 1993-10-28 DE DE69331434T patent/DE69331434T2/de not_active Expired - Lifetime
- 1993-10-29 CA CA002102043A patent/CA2102043C/en not_active Expired - Lifetime
- 1993-10-30 KR KR1019930022880A patent/KR100393297B1/ko not_active IP Right Cessation
-
1995
- 1995-06-06 US US08/471,206 patent/US5683893A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2102043C (en) | 2007-09-18 |
EP0595334A2 (en) | 1994-05-04 |
CA2102043A1 (en) | 1994-05-01 |
DK0595334T3 (da) | 2002-04-29 |
EP0595334A3 (en) | 1995-05-31 |
US5683893A (en) | 1997-11-04 |
JPH06189769A (ja) | 1994-07-12 |
KR100393297B1 (ko) | 2004-10-20 |
TW343997B (en) | 1998-11-01 |
DE69331434T2 (de) | 2002-09-05 |
DE69331434D1 (de) | 2002-02-14 |
KR940009334A (ko) | 1994-05-20 |
EP0595334B1 (en) | 2002-01-09 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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