ES2160191T3 - Deteccion de listerias, mediante bacteriofagos recombinantes. - Google Patents

Deteccion de listerias, mediante bacteriofagos recombinantes.

Info

Publication number
ES2160191T3
ES2160191T3 ES96107225T ES96107225T ES2160191T3 ES 2160191 T3 ES2160191 T3 ES 2160191T3 ES 96107225 T ES96107225 T ES 96107225T ES 96107225 T ES96107225 T ES 96107225T ES 2160191 T3 ES2160191 T3 ES 2160191T3
Authority
ES
Spain
Prior art keywords
proteins
genero
determination
bacteria
dna vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96107225T
Other languages
English (en)
Inventor
Martin J Dr Lossner
Siegfried Prof Dr Scherer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xenogen Corp
Original Assignee
Xenogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xenogen Corp filed Critical Xenogen Corp
Application granted granted Critical
Publication of ES2160191T3 publication Critical patent/ES2160191T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10111—Myoviridae
    • C12N2795/10141—Use of virus, viral particle or viral elements as a vector
    • C12N2795/10143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION TRATA DE UN PROCEDIMIENTO DE DETERMINACION DE BACTERIAS DEL GENERO LISTERIA QUE COMPRENDE LOS SIGUIENTES PASOS: (A) PREPARACION DE UN VECTOR DE ADN OBTENIDO MEDIANTE LAS TECNICAS DE RECOMBINACION, CONTENIENDO UN SISTEMA GENETICO QUE COMPRENDE ADN, QUE CODIFICA LA EXPRESION DE UNA O VARIAS PROTEINAS, NO SIENDO LAS PROTEINAS NINGUN PRODUCTO GENICO DE BACTERIAS DEL GENERO LISTERIA, Y SE PUEDE DETERMINAR LA PRESENCIA DE LAS PROTEINAS POR UNA REACCION DE DETERMINACION, INFECTANDO EL VECTOR DE ADN LAS BACTERIAS DEL GENERO LISTERIA, Y PASANDO POR TANTO EL SISTEMA GENETICO A LAS BACTERIAS; (B) MEZCLA DE LA MUESTRA CON EL CITADO VECTOR DE ADN EN UNAS CONDICIONES QUE PERMITAN LA INFECCION DE BACTERIAS DEL GENERO LISTERIA POR EL VECTOR DE ADN; (C) EXPRESION DE LAS PROTEINAS A DETERMINAR EN LAS BACTERIAS DEL GENERO LISTERIA; (D) DETERMINACION DE LAS PROTEINAS A DETERMINAR, DETERMINANDO LA PRESENCIA DE BACTERIAS DEL GENERO LISTERIA; ASI COMO LOS VECTORES DE ADN APROPIADOS PARA ESTE PROCEDIMIENTO DE DETERMINACION Y COMBINACION DE REACTIVOS.
ES96107225T 1995-05-18 1996-05-08 Deteccion de listerias, mediante bacteriofagos recombinantes. Expired - Lifetime ES2160191T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19517940A DE19517940A1 (de) 1995-05-18 1995-05-18 Nachweis von Listerien mittels rekombinanter Bakteriophagen

Publications (1)

Publication Number Publication Date
ES2160191T3 true ES2160191T3 (es) 2001-11-01

Family

ID=7762052

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96107225T Expired - Lifetime ES2160191T3 (es) 1995-05-18 1996-05-08 Deteccion de listerias, mediante bacteriofagos recombinantes.

Country Status (5)

Country Link
US (1) US5824468A (es)
EP (1) EP0743366B1 (es)
DE (2) DE19517940A1 (es)
DK (1) DK0743366T3 (es)
ES (1) ES2160191T3 (es)

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US6649143B1 (en) 1994-07-01 2003-11-18 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
DE60014987T2 (de) * 1999-02-22 2005-11-10 Matsushita Electric Industrial Co., Ltd., Kadoma Verfahren zum Nachweis von Bakterien
FI107167B (fi) 1999-06-07 2001-06-15 Marko Virta Menetelmä mikro-organismin kasvun ja kuolemisen määrittämiseksi
PT1198713E (pt) 1999-07-30 2006-06-30 Profos Ag Deteccao e identificacao de estirpes bacterianas
US20030096315A1 (en) 2000-05-03 2003-05-22 Sanders Mitchell C. Device for detecting bacterial contamination and method of use
US20020192676A1 (en) * 2001-06-18 2002-12-19 Madonna Angelo J. Method for determining if a type of bacteria is present in a mixture
WO2003035889A2 (en) * 2001-07-13 2003-05-01 Investigen, Inc. Compositions and methods for bacteria detection
US7232564B2 (en) * 2001-07-18 2007-06-19 Instytut Immunologii I Terapii Doswiadczal-Nej Pan Methods of polyvalent bacteriophage preparation for the treatment of bacterial infections
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EP1540018B1 (en) * 2002-04-12 2010-05-26 Colorado School Of Mines Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells
PL215172B1 (pl) * 2003-01-31 2013-10-31 Ethicon Inc Sposób wykrywania Escherichia coli, biosensor do wykrywania Escherichia coli i zestaw do wykrywania Escherichia coli
CA2557612C (en) * 2003-11-03 2014-06-17 Ethicon, Inc. Methods, peptides and biosensors useful for detecting a broad spectrum of bacteria
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WO2006105414A2 (en) * 2005-03-31 2006-10-05 Colorado School Of Mines Apparatus and method for detecting microscopic organisms using microphage
US20070178450A1 (en) * 2006-01-27 2007-08-02 Microphage (Tm) Incorporation Method and apparatus for determining level of microorganisms using bacteriophage
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CN108884498A (zh) * 2016-01-18 2018-11-23 美国控股实验室公司 使用感染原快速检测微生物的方法和系统
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Also Published As

Publication number Publication date
EP0743366B1 (de) 2001-07-11
DK0743366T3 (da) 2001-10-01
DE19517940A1 (de) 1996-11-21
US5824468A (en) 1998-10-20
EP0743366A1 (de) 1996-11-20
DE59607254D1 (de) 2001-08-16

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