ES2134852T3 - Una linea celular tratada por ingenieria genetica para detectar el virus herpes simple infeccioso y metodo para ello. - Google Patents

Una linea celular tratada por ingenieria genetica para detectar el virus herpes simple infeccioso y metodo para ello.

Info

Publication number
ES2134852T3
ES2134852T3 ES93916604T ES93916604T ES2134852T3 ES 2134852 T3 ES2134852 T3 ES 2134852T3 ES 93916604 T ES93916604 T ES 93916604T ES 93916604 T ES93916604 T ES 93916604T ES 2134852 T3 ES2134852 T3 ES 2134852T3
Authority
ES
Spain
Prior art keywords
herpes virus
cell line
infectious
herpes
individual
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93916604T
Other languages
English (en)
Inventor
Paul D Olivo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Washington University in St Louis WUSTL
Original Assignee
University of Washington
Washington University in St Louis WUSTL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington, Washington University in St Louis WUSTL filed Critical University of Washington
Application granted granted Critical
Publication of ES2134852T3 publication Critical patent/ES2134852T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0093—Oxidoreductases (1.) acting on CH or CH2 groups (1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90209—Oxidoreductases (1.) acting on NADH or NADPH (1.6), e.g. those with a heme protein as acceptor (1.6.2) (general), Cytochrome-b5 reductase (1.6.2.2) or NADPH-cytochrome P450 reductase (1.6.2.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physiology (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE DESCRIBE UN PRUEBA DE DIAGNOSTICO PARA DETECTAR LA PRESENCIA DE UN VIRUS DE HERPES INFECCIOSO EN UNA INDIVIDUO Y UNA LINEA CELULA GENETICAMENTE MANIPULADA PARA SU USO EN TAL PRUEBA.. LA LINEA CELULAR USADA EN LA PRUEBA EXPRESA UN GEN REPETIDOR SOLAMENTE SI EL VIRUS DE HERPES INFECCIOSO ESTA PRESENTE EN EL INDIVIDUO. LA PRUEBA CONSISTE EN INOCULAR UNA CELULA TRANSFECTADA DE ADN CON UN INDIVIDUO SOSPECHOSO DE CONTENER UN VIRUS DE HERPES, PERMITIR QUE PROCESADA UN PERIODO DE TIEMPO SUFICIENTE PARA EL CICLO INFECCIOSO DEL VIRUS DEL HERPES, Y DETECTAR Y CUANTIFICAR EL NUMERO DE CELULAS INFECTADAS DEL VIRUS DEL HERPES PARA DETERMINAR EL NUMERO DE VIRIONES DE VIRUS DE HERPES INFECCIOSOS EN EL INDIVIDUO. LA LINEA CELULAR ES UNA LINEA CELULAR TRANSFECTADA DE ADN SUSCEPTIBLE DE INFECCION POR UN VIRUS DE HERPES QUE ESTA ESTABLEMENTE TRANSFORMADO CON UN GEN QUIMERICO QUE COMPRENDE UN PROMOTOR INDUCIBLE DE VIRUS DE HERPES Y UN GEN QUE CODIFICA UNA ENZIMA, SIENDO LA EXPRESION DE LA ENZIMA DEPENDIENTEDE Y CUANTITATIVAMENTE PROPORCIONAL A LA PRESENCIA DEL VIRUS DEL HERPES. TAMBIEN SE PREVE UN KIT PARA TAL ENSAYO.
ES93916604T 1992-06-18 1993-06-17 Una linea celular tratada por ingenieria genetica para detectar el virus herpes simple infeccioso y metodo para ello. Expired - Lifetime ES2134852T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/900,279 US5418132A (en) 1992-06-18 1992-06-18 Genetically engineered cell line for detecting infectious herpes simplex virus and method therefore

Publications (1)

Publication Number Publication Date
ES2134852T3 true ES2134852T3 (es) 1999-10-16

Family

ID=25412274

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93916604T Expired - Lifetime ES2134852T3 (es) 1992-06-18 1993-06-17 Una linea celular tratada por ingenieria genetica para detectar el virus herpes simple infeccioso y metodo para ello.

Country Status (9)

Country Link
US (1) US5418132A (es)
EP (1) EP0646124B1 (es)
JP (1) JP3544973B2 (es)
AT (1) ATE180836T1 (es)
AU (1) AU680423B2 (es)
CA (1) CA2137809C (es)
DE (1) DE69325173T2 (es)
ES (1) ES2134852T3 (es)
WO (1) WO1994000468A1 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958676A (en) * 1992-06-18 1999-09-28 Washington University Genetically engineered cell lines for detecting infectious herpesvirus and methods therefor
US5733720A (en) * 1992-06-18 1998-03-31 Washington University Genetically engineered cell lines for detecting infectious herpesvirus and methods therefor
US6649143B1 (en) * 1994-07-01 2003-11-18 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
US7255851B2 (en) * 1994-07-01 2007-08-14 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
SE512866C2 (sv) 1995-02-21 2000-05-29 Volvo Ab Anordning för analys av avgaser
US5945276A (en) * 1996-04-10 1999-08-31 Signal Pharmaceuticals, Inc. Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression
US8349602B1 (en) 1996-04-19 2013-01-08 Xenogen Corporation Biodetectors targeted to specific ligands
US5939253A (en) * 1996-04-26 1999-08-17 Diagnostic Hybrids, Inc. Compositions and methods for detecting viral infection
US5910411A (en) * 1996-09-30 1999-06-08 Signal Pharmaceuticals, Inc. Method for identifying agents that modulate transcription of human cytomegalovirus polymerase
US7572582B2 (en) 1997-09-12 2009-08-11 Exiqon A/S Oligonucleotide analogues
US6051385A (en) * 1997-10-22 2000-04-18 The Regents Of The University Of Michigan Compositions and methods for identifying and testing therapeutics against HSV infection
US20060094105A1 (en) 1998-04-24 2006-05-04 University Hospitals Of Cleveland Mixed cell diagnostic systems for detection of respiratory, herpes and enteric viruses
US6168915B1 (en) * 1998-04-24 2001-01-02 Diagnostic Hybrids, Inc. Mixed cell diagnostic systems
US6946291B2 (en) * 1998-04-24 2005-09-20 University Hospitals Of Cleveland Mixed cell diagnostic systems
US6270958B1 (en) 1998-10-29 2001-08-07 Washington University Detection of negative-strand RNA viruses
US6136538A (en) * 1999-01-08 2000-10-24 Washington University Silent inducible virus replicons and uses thereof
US6326480B1 (en) 1999-01-19 2001-12-04 Signal Pharmaceuticals, Inc. Antisense reporter system for assaying RNA virus replication
CA2548569C (en) * 2003-12-08 2012-07-10 Loma Linda University Methods for detecting dna viruses
US7435588B2 (en) * 2005-09-20 2008-10-14 Diagnostic Hybrids, Inc. Systems for detection and production of respiratory, herpes and enteric viruses
CN116948988A (zh) 2016-01-29 2023-10-27 西里昂生物技术有限责任公司 基于aav的条件表达系统

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8626862D0 (en) * 1986-11-11 1986-12-10 Jefferson R A Gene fusion
US5070012A (en) * 1988-03-30 1991-12-03 The Board Of Trustees Of The Leland Stanford Junior University Monitoring of cells and trans-activating transcription elements
WO1990002797A1 (en) * 1988-09-15 1990-03-22 North Carolina State University Self-inactivating, replication defective, retroviral vectors and helper cells for producing the same

Also Published As

Publication number Publication date
EP0646124B1 (en) 1999-06-02
CA2137809A1 (en) 1994-01-06
EP0646124A1 (en) 1995-04-05
US5418132A (en) 1995-05-23
AU4640193A (en) 1994-01-24
CA2137809C (en) 2002-09-03
WO1994000468A1 (en) 1994-01-06
JPH07508409A (ja) 1995-09-21
AU680423B2 (en) 1997-07-31
DE69325173T2 (de) 2000-01-20
EP0646124A4 (en) 1996-10-23
DE69325173D1 (de) 1999-07-08
ATE180836T1 (de) 1999-06-15
JP3544973B2 (ja) 2004-07-21

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