ES2150064T3 - Un sustrato recombinante artificial (ragg-1) y agrecano nativo para estudiar la actividad proteolitica de la "agrecanasa" en sistemas de cultivo celular. - Google Patents
Un sustrato recombinante artificial (ragg-1) y agrecano nativo para estudiar la actividad proteolitica de la "agrecanasa" en sistemas de cultivo celular.Info
- Publication number
- ES2150064T3 ES2150064T3 ES96120949T ES96120949T ES2150064T3 ES 2150064 T3 ES2150064 T3 ES 2150064T3 ES 96120949 T ES96120949 T ES 96120949T ES 96120949 T ES96120949 T ES 96120949T ES 2150064 T3 ES2150064 T3 ES 2150064T3
- Authority
- ES
- Spain
- Prior art keywords
- aggrecanase
- study
- recombinant substrate
- recombinant
- human igg1
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4725—Proteoglycans, e.g. aggreccan
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- General Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
LA INVENCION SE REFIERE A UN SUSTRATO RECOMBINANTE PARA SISTEMAS DE COMPROBACION IN VITRO DE AGRECANASA QUE CONTIENEN UN GRUPO DE ELEMENTOS ESTRUCTURALES QUE COMPRENDE LA SECUENCIA DE SEÑAL DE CD5, EL EPITOPE FLAG PARA LA DETECCION DE ANTICUERPO MONOCLONAL M1, EL CAMPO INTERGLOBULAR DE AGRECAN HUMANO, LA REGION ESENCIAL DE IGG1 HUMANO, LA REGION CH2 DE IGG1 HUMANO Y LA REGION CH3 DE IGG1 HUMANO; UN ADN QUE CODIFICA EL SUSTRATO RECOMBINANTE; VECTORES Y CELULAS HUESPED QUE CONTIENEN DICHO ADN Y USO DEL SUSTRATO RECOMBINANTE PARA ESTUDIAR LA ACTIVIDAD DE AGRECANASA PARA DETECTAR NUEVOS EMPLAZAMIENTOS DE SEPARACION ENZIMATICA, PARA PURIFICAR AGRECANASA CROMATOGRAFICAMENTE, PARA CLONAR EL CADN DE AGRECANASA MEDIANTE CLONACION FUNCIONAL, PARA INVESTIGAR UN INHIBIDOR DE AGRECANASA; UN METODO PARA SUPERVISAR EL ESTABLECIMIENTO O PROGRESION DE OSTEORTRITIS Y UNA AYUDA DE DIAGNOSTICO QUE CONTIENE EL SUSTRATO RECOMBINANTE.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP96100682 | 1996-01-18 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2150064T3 true ES2150064T3 (es) | 2000-11-16 |
Family
ID=8222415
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96120949T Expired - Lifetime ES2150064T3 (es) | 1996-01-18 | 1996-12-27 | Un sustrato recombinante artificial (ragg-1) y agrecano nativo para estudiar la actividad proteolitica de la "agrecanasa" en sistemas de cultivo celular. |
Country Status (11)
Country | Link |
---|---|
US (2) | US5872209A (es) |
EP (1) | EP0785274B1 (es) |
JP (1) | JP3973253B2 (es) |
AT (1) | ATE196652T1 (es) |
AU (1) | AU713828B2 (es) |
CA (1) | CA2195385C (es) |
DE (1) | DE69610483T2 (es) |
DK (1) | DK0785274T3 (es) |
ES (1) | ES2150064T3 (es) |
GR (1) | GR3034649T3 (es) |
PT (1) | PT785274E (es) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK0785274T3 (da) * | 1996-01-18 | 2000-12-27 | Aventis Pharma Gmbh | Kunstigt rekombinant substrat (rAGG-1) og nativ aggrecan til undersøgelse af den proteolytiske aktivitet af "aggrecanase" i |
EP0998572A2 (en) | 1997-07-25 | 2000-05-10 | Du Pont Pharmaceuticals Company | Aggrecan degrading metallo proteases |
WO2000005256A1 (en) * | 1998-07-24 | 2000-02-03 | Du Pont Pharmaceuticals Company | Assays and peptide substrate for determining aggrecan degrading metallo protease activity |
US6649377B1 (en) | 1999-05-10 | 2003-11-18 | Syntex (U.S.A.) Llc | Human aggrecanase and nucleic acid compositions encoding the same |
AU2002335747B2 (en) | 2001-09-15 | 2009-01-29 | Rush University Medical Center | Stratified cartilage tissue and methods to engineer same |
US20030165473A1 (en) * | 2001-11-09 | 2003-09-04 | Rush-Presbyterian-St. Luke's Medical Center | Engineered intervertebral disc tissue |
DE102004029470A1 (de) * | 2004-06-18 | 2006-01-05 | InViTek Gesellschaft für Biotechnik & Biodesign mbH | Verfahren zur Bestimmung von Aggrekanase-Aktivitäten |
DE102005005972A1 (de) | 2005-02-09 | 2006-08-17 | Sanofi-Aventis Deutschland Gmbh | Verwendung von Thiopeptoliden zur Aktivitätsbestimmung von ADAM-T8 Proteasen |
CA2769663A1 (en) * | 2008-07-28 | 2010-02-04 | Dorit Arad | Methods and compositions for detection of a pathogen, disease, medical condition, or biomarker thereof |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5427954A (en) * | 1992-04-29 | 1995-06-27 | Shriner's Hospitals For Crippled Children | Compositions and methods for detection and treatment of human osteoarthritis |
GB9310978D0 (en) * | 1993-05-27 | 1993-07-14 | Zeneca Ltd | Compounds |
US5851523A (en) * | 1994-03-24 | 1998-12-22 | Ludwig Institute For Cancer Research. | Isolated, peptides derived from MAGE tumor rejection antigen precursors which complex with HLA-A2 molecules and uses thereof |
SE9500023L (sv) * | 1994-05-17 | 1995-11-18 | Beki Ab | Sätt att detektera cancer |
DK0785274T3 (da) * | 1996-01-18 | 2000-12-27 | Aventis Pharma Gmbh | Kunstigt rekombinant substrat (rAGG-1) og nativ aggrecan til undersøgelse af den proteolytiske aktivitet af "aggrecanase" i |
-
1996
- 1996-12-27 DK DK96120949T patent/DK0785274T3/da active
- 1996-12-27 AT AT96120949T patent/ATE196652T1/de active
- 1996-12-27 DE DE69610483T patent/DE69610483T2/de not_active Expired - Lifetime
- 1996-12-27 EP EP96120949A patent/EP0785274B1/en not_active Expired - Lifetime
- 1996-12-27 PT PT96120949T patent/PT785274E/pt unknown
- 1996-12-27 ES ES96120949T patent/ES2150064T3/es not_active Expired - Lifetime
-
1997
- 1997-01-16 AU AU10199/97A patent/AU713828B2/en not_active Ceased
- 1997-01-17 CA CA2195385A patent/CA2195385C/en not_active Expired - Fee Related
- 1997-01-17 US US08/784,512 patent/US5872209A/en not_active Expired - Lifetime
- 1997-01-20 JP JP02097097A patent/JP3973253B2/ja not_active Expired - Fee Related
-
1998
- 1998-10-21 US US09/176,228 patent/US6180334B1/en not_active Expired - Lifetime
-
2000
- 2000-10-20 GR GR20000402336T patent/GR3034649T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
DE69610483D1 (de) | 2000-11-02 |
AU1019997A (en) | 1997-07-24 |
EP0785274B1 (en) | 2000-09-27 |
CA2195385C (en) | 2010-07-27 |
DE69610483T2 (de) | 2001-06-13 |
JPH09191889A (ja) | 1997-07-29 |
DK0785274T3 (da) | 2000-12-27 |
JP3973253B2 (ja) | 2007-09-12 |
GR3034649T3 (en) | 2001-01-31 |
CA2195385A1 (en) | 1997-07-19 |
ATE196652T1 (de) | 2000-10-15 |
PT785274E (pt) | 2001-01-31 |
EP0785274A1 (en) | 1997-07-23 |
US5872209A (en) | 1999-02-16 |
US6180334B1 (en) | 2001-01-30 |
AU713828B2 (en) | 1999-12-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE10075010I1 (de) | Fusionsproteine mit immunglobulinanteilen ihre Herstellung und Verwendung | |
FI961251A (fi) | Ihmisten metabotrooppiset glutamaattireseptorialatyypit(HMR4, HMR6, HMR7)ja niihin liittyvät DNA-yhdisteet | |
PT914345E (pt) | Ligacao de anticorpos a receptores (ccr2) de proteina 1 de quimio-atraccao de monocitos (pqm-1) | |
DK0726952T3 (da) | Receptor benævnt ACT-4 på overfladen af aktiverede T-celler | |
DK0943097T3 (da) | Fremgangsmåde til at påvise IgE | |
TR200102715T2 (tr) | Beşeri IL-12'yi bağlayan beşeri antikorlar ve bunları üretmek için yöntemler. | |
DK0914155T3 (da) | Monoklonale antistoffer, der er spepcifikke for det ekstracellulære domæne af prostataspecifikt membran-antigen | |
ES2150064T3 (es) | Un sustrato recombinante artificial (ragg-1) y agrecano nativo para estudiar la actividad proteolitica de la "agrecanasa" en sistemas de cultivo celular. | |
SE9500023D0 (sv) | Method of detecting cancer | |
WO1999046379A3 (en) | Human receptor proteins; related reagents and methods | |
ES2121358T3 (es) | Anticuerpos monoclonales humanizados contra interleuquina-4 humana. | |
DK1181366T3 (da) | Mammale receptorproteiner, beslægtede reagenser og fremgangsmåder | |
DK0991945T3 (da) | Fremgangsmåde til identifikation af tumor-antigener med antistoffer i serum | |
DE69728897D1 (de) | Saeugetierchemokine | |
DE59710668D1 (de) | Monoklonale antikörper gegen das epitop ypydvpdya, verfahren zu deren herstellung und ihre verwendung | |
DK0939769T3 (da) | Vertebrat-Smoothened-proteiner | |
DK0495910T3 (da) | Fremstilling og anvendelse af humant nm23-H2-protein og derimod rettede antistoffer | |
DK1572931T3 (da) | Fremgangsmåder og sammensætninger til modulering og påvisning af WISP-aktivitet | |
MX9302549A (es) | Proteina receptora especifica para la region csvt cg de la trombospondina y metodo para su purificacion y caracterizacion. | |
DK0583352T3 (da) | hGPIIB fragmenter og deres anvendelse i in vitro metoder til bestemmelse af in vivo thrombotiske tilstande | |
WO2001065262A3 (en) | Reagents and methods useful for detecting diseases of the breast | |
MX9700469A (es) | Un substrato recombinante artificial (ragg 1) y agrecano natural para estudiar la actividad proteolitica de "agrecanasa" en sistemas de cultivos celulares. | |
WO1999019480A3 (en) | Human receptor proteins; related reagents and methods |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 785274 Country of ref document: ES |