ES2144110T3 - Oligonucleotido quimera y su utilizacion para la obtencion de transcritos de un acido nucleico. - Google Patents

Oligonucleotido quimera y su utilizacion para la obtencion de transcritos de un acido nucleico.

Info

Publication number
ES2144110T3
ES2144110T3 ES95402150T ES95402150T ES2144110T3 ES 2144110 T3 ES2144110 T3 ES 2144110T3 ES 95402150 T ES95402150 T ES 95402150T ES 95402150 T ES95402150 T ES 95402150T ES 2144110 T3 ES2144110 T3 ES 2144110T3
Authority
ES
Spain
Prior art keywords
sequence
oligonucleotidic
nucleic acid
segment
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95402150T
Other languages
English (en)
Inventor
Francoise Guillou-Bonnici
Pierre Levasseur
Philippe Cleuziat
William Mcallister
Francois Mallet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2144110T3 publication Critical patent/ES2144110T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

OLIGONUCLEOTIDO QUIMERICO QUE PUEDE SER UTILIZADO EN UN PROCESO DE OBTENCION DE TRANSCRITOS Y/O DE AMPLIFICACION DE UNA SECUENCIA-DIANA DE UN ACIDO NUCLEICO QUE COMPRENDE, EN SU EXTREMO 3'', UNA SECUENCIA CORRIENTE ABAJO, COMPRENDIENDO DICHO OLIGONUCLEOTIDO SUCESIVAMENTE, DE 5'' HACIA 3'', UN PRIMER SEGMENTO OLIGONUCLEOTIDICO, DE TIPO ADN, QUE COMPRENDE UNA SECUENCIA SENTIDO DE UN PROMOTOR DE UNA ARN POLIMERASA, UN SEGUNDO SEGMENTO OLIGONUCLEOTIDICO, DE TIPO ADN, CAPAZ DE HIBRIDARSE CON DICHA SECUENCIA CORRIENTE ABAJO, Y UN TERCER SEGMENTO OLIGONUCLEOTIDICO, DE TIPO ARN, CAPAZ DE HIDRIDARSE CON UNA PARTE DE LA SECUENCIA DIANA CONTIGUA A DICHA SECUENCIA CORRIENTE ABAJO, ESTANDO EL TERCER SEGMENTO BLOQUEADO EN 3''. CON EL OLIGONUCLEOTIDO QUIMERICO Y UN SISTEMA ENZIMATICO QUE CONTIENE UNA ACTIVIDAD ADN POLIMERASA, UNA ACTIVIDAD ARN POLIMERASA Y POR EJEMPLO UNA ACTIVIDAD DE RNASA H, SE OBTIENEN PRODUCTOS DE TRANSCRIPCION DE LA DIANA. AÑADIENDO UN SEGUNDO OLIGONUCLEOTIDO QUIMERICO CAPAZ DE HIBRIDARSE CON EL COMPLEMENTARIO DE LA DIANA, SE OBTIENE UNA AMPLIFICACION CICLICA DEL OBJETIVO Y DE SU COMPLEMENTARIO.
ES95402150T 1994-09-26 1995-09-26 Oligonucleotido quimera y su utilizacion para la obtencion de transcritos de un acido nucleico. Expired - Lifetime ES2144110T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9411455A FR2724934B1 (fr) 1994-09-26 1994-09-26 Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique

Publications (1)

Publication Number Publication Date
ES2144110T3 true ES2144110T3 (es) 2000-06-01

Family

ID=9467268

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95402150T Expired - Lifetime ES2144110T3 (es) 1994-09-26 1995-09-26 Oligonucleotido quimera y su utilizacion para la obtencion de transcritos de un acido nucleico.

Country Status (8)

Country Link
US (1) US5744308A (es)
EP (1) EP0721988B1 (es)
AT (1) ATE189264T1 (es)
CA (1) CA2159064A1 (es)
DE (1) DE69514773T2 (es)
DK (1) DK0721988T3 (es)
ES (1) ES2144110T3 (es)
FR (1) FR2724934B1 (es)

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FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
EP0941314A1 (fr) 1996-08-02 1999-09-15 Bio Merieux Procede d'amplification d'une sequence d'un acide nucleique cible
ID26107A (id) 1998-02-27 2000-11-23 Pamgene Bv Metoda untuk amplifikasi tak spesifik dari asam nukleat
FR2780059B1 (fr) * 1998-06-17 2002-10-11 Bio Merieux Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US6677445B1 (en) 1999-08-27 2004-01-13 Chiron Corporation Chimeric antisense oligonucleotides and cell transfecting formulations thereof
JP3929775B2 (ja) 1999-09-13 2007-06-13 ニューゲン テクノロジーズ， インコーポレイテッド ポリヌクレオチド配列の線形等温増幅のための方法および組成物
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1356094B1 (en) * 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
NZ524894A (en) * 2000-09-02 2005-03-24 Gruenenthal Chemie Antisense oligonucleotides against VR1
US6919189B2 (en) 2000-12-11 2005-07-19 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing a hairpin for nucleic acid amplification
EP1427847B1 (en) * 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
IL153504A0 (en) * 2001-03-09 2003-07-06 Nugen Technologies Inc Methods and compositions for amplification of rna sequences
WO2002072773A2 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
EP1420069A4 (en) * 2001-08-20 2005-11-02 Takara Bio Inc nucleic acid amplification
US7414111B2 (en) * 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
DE60234369D1 (de) * 2001-09-19 2009-12-24 Alexion Pharma Inc Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation
US7045319B2 (en) * 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
ATE405676T1 (de) * 2002-03-11 2008-09-15 Nugen Technologies Inc Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
GB0230238D0 (en) * 2002-12-28 2003-02-05 Fu Guoliang Combined exponential and linear amplification
EP1556517B1 (en) * 2002-10-30 2006-03-08 PamGene B.V. Improved methods for generating multiple rna copies
US7413857B2 (en) 2002-11-21 2008-08-19 Epicentre Technologies Methods for using riboprimers for strand displacement replication of target sequences
AU2003301056A1 (en) * 2002-12-20 2004-07-22 Q-Rna, Inc. Methods and kits for making double stranded ribonucleic acids
CA2521084A1 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
CA2544367A1 (en) * 2003-10-29 2005-05-12 Ribomed Biotechnologies, Inc. Compositions, methods and detection technologies for reiterative oligonucleotide synthesis
EP1929046B1 (en) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
EP1921156A1 (en) * 2006-11-10 2008-05-14 bioMerieux B.V. Improved multiplex nucleic acid amplification using blocked primers
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
AU2009242546B2 (en) 2008-04-30 2015-01-22 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
EP2297346B1 (en) 2008-05-15 2015-04-15 Ribomed Biotechnologies, Inc. METHODS AND REAGENTS FOR DETECTING CpG METHYLATION WITH A METHYL CpG BINDING PROTEIN (MBP)
US8211644B2 (en) * 2008-07-13 2012-07-03 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
WO2010107716A2 (en) 2009-03-15 2010-09-23 Ribomed Biotechnologies, Inc. Abscription based molecular detection
WO2011060014A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US20130217071A1 (en) 2011-12-30 2013-08-22 Luz Montesclaros Methods and compositions for performing nucleic acid amplification reactions
CA2892818A1 (en) 2012-11-28 2014-06-05 Abwiz Bio, Inc. Preparation of gene-specific templates for the use in single primer amplification
AU2013381709A1 (en) * 2013-03-15 2015-10-01 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
GB2595076B8 (en) * 2018-11-21 2023-08-23 Agilent Technologies Inc Methods for targeted nucleic acid library formation
JP2023545400A (ja) 2020-10-02 2023-10-30 レクソジェン・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング 転写核酸の生成方法及び手段
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AU670116B2 (en) * 1992-08-04 1996-07-04 Gen-Probe Incorporated Nucleic acid sequence amplification

Also Published As

Publication number Publication date
DE69514773T2 (de) 2000-09-07
ATE189264T1 (de) 2000-02-15
EP0721988A1 (fr) 1996-07-17
FR2724934A1 (fr) 1996-03-29
DE69514773D1 (de) 2000-03-02
DK0721988T3 (da) 2000-07-24
EP0721988B1 (fr) 2000-01-26
FR2724934B1 (fr) 1997-01-24
US5744308A (en) 1998-04-28
CA2159064A1 (fr) 1996-03-27

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