ES2140705T3 - Aparato y metodo de separacion de celulas. - Google Patents
Aparato y metodo de separacion de celulas.Info
- Publication number
- ES2140705T3 ES2140705T3 ES95931685T ES95931685T ES2140705T3 ES 2140705 T3 ES2140705 T3 ES 2140705T3 ES 95931685 T ES95931685 T ES 95931685T ES 95931685 T ES95931685 T ES 95931685T ES 2140705 T3 ES2140705 T3 ES 2140705T3
- Authority
- ES
- Spain
- Prior art keywords
- methods
- cell separation
- disclosed
- cell
- constriction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
- C12N5/0093—Purging against cancer cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0439—White blood cells; Leucocytes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0605—Valves, specific forms thereof check valves
Abstract
SE PRESENTAN METODOS PARA ENRIQUECER TIPOS DE CELULAS ESPECIFICOS A PARTIR DE MEZCLAS DE CELULAS. INCLUIDOS EN LOS METODOS ESTAN EL USO DE UN MEDIO DE SEPARACION DE CELULAS DEFINIDOS DE FORMA PRECISA, UN APARATO DE SEPARACION DE CELULAS QUE INCLUYE UNA CONSTRICCION QUE DEFINE UNA REGION INFERIOR Y UN MEDIO DE SEPARACION DE CELULAS DEFINIDOS. LA CONSTRICCION EVITA LA MEZCLA ENTRE LAS PARTES SUPERIOR E INFERIOR DEL TUBO. TAMBIEN SE PRESENTA UNA TECNICA DE SEPARACION DE CELULAS AJUSTADA POR DENSIDAD UTILIZADA PARA MEJORAR LOS METODOS ANTERIORES. ADEMAS, LA INVENCION INCLUYE METODOS ESPECIFICOS PARA AISLAR CELULAS PROGENITORAS HEMATOPOIETICAS CD34+, CELULAS FETALES NUCLEADAS, Y CELULAS TUMORALES DE LA MAMA. LOS METODOS DE AISLAMIENTO SON UTILES EN DIFERENTES REGIMENES DE DIAGNOSTICO Y TERAPEUTICOS.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/299,468 US5646004A (en) | 1994-08-31 | 1994-08-31 | Methods for enriching fetal cells from maternal body fluids |
US08/299,465 US5648223A (en) | 1994-08-31 | 1994-08-31 | Methods for enriching breast tumor cells |
US08/299,469 US5474687A (en) | 1994-08-31 | 1994-08-31 | Methods for enriching CD34+ human hematopoietic progenitor cells |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2140705T3 true ES2140705T3 (es) | 2000-03-01 |
Family
ID=27404658
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95931685T Expired - Lifetime ES2140705T3 (es) | 1994-08-31 | 1995-08-31 | Aparato y metodo de separacion de celulas. |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0778944B1 (es) |
JP (1) | JP3397795B2 (es) |
AT (1) | ATE186398T1 (es) |
AU (1) | AU700743B2 (es) |
CA (1) | CA2198607C (es) |
DE (1) | DE69513188T2 (es) |
DK (1) | DK0778944T3 (es) |
ES (1) | ES2140705T3 (es) |
HK (1) | HK1013862A1 (es) |
NZ (1) | NZ292756A (es) |
WO (1) | WO1996007097A1 (es) |
Families Citing this family (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5577513A (en) * | 1994-08-31 | 1996-11-26 | Activated Cell Therapy, Inc. | Centrifugation syringe, system and method |
GB9422504D0 (en) | 1994-11-08 | 1995-01-04 | Robertson Patricia M B | Blood testing |
US6582904B2 (en) | 1995-11-16 | 2003-06-24 | Michael W. Dahm | Method of quantifying tumour cells in a body fluid and a suitable test kit |
ATE530253T1 (de) * | 1995-12-11 | 2011-11-15 | Dendreon Corp | Zusammensetzung, kit und verfahren zur zelltrennung |
EP0959989B1 (en) | 1997-02-14 | 2003-11-12 | Dendreon Corporation | Cell washing device and method |
US6544751B1 (en) | 1997-04-08 | 2003-04-08 | Pall Corporation | Methods of harvesting rare cells from blood products |
IT1295939B1 (it) * | 1997-10-31 | 1999-05-28 | Giammaria Sitar | Dispositivo e metodo per la separazione di cellule umane od animali aventi densita' diverse da dispersioni cellulari che le contengono |
AU9233698A (en) * | 1997-11-22 | 1999-06-17 | Robert A. Levine | Method for the detection, identification, enumeration and confirmation of circulating cancer cells and/or hematologic progenitor cells in whole blood |
DE19804372A1 (de) | 1998-02-04 | 1999-08-05 | Michael W Dr Dr Dahm | Verfahren zur quantitativen Bestimmung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits |
DE19904267A1 (de) | 1999-02-03 | 2000-08-10 | Michael W Dahm | Verfahren zur Anreicherung von Tumorzellen aus einer Körperflüssigkeit und dazu geeigneter Kit |
US7030228B1 (en) | 1999-11-15 | 2006-04-18 | Miltenyi Biotec Gmbh | Antigen-binding fragments specific for dendritic cells, compositions and methods of use thereof antigens recognized thereby and cells obtained thereby |
US7947236B2 (en) | 1999-12-03 | 2011-05-24 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
WO2003050532A1 (fr) * | 2001-12-11 | 2003-06-19 | Netech Inc. | Systeme de separation de cellules sanguines |
EP1677889A4 (en) | 2003-09-30 | 2009-01-14 | Capitalbio Corp | APPARATUS AND METHOD FOR CENTRIFUGAL SEPARATION |
ITRM20030467A1 (it) * | 2003-10-10 | 2005-04-11 | Advance Holdings Ltd | Contenitore monouso per la centrifugazione ed il trattamento di un materiale biologico fluido. |
WO2007055573A1 (en) * | 2005-11-14 | 2007-05-18 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for removing a fluid from a container and device therefor |
JP2008220319A (ja) * | 2007-03-15 | 2008-09-25 | Kaneka Corp | 遠心分離用容器および支持器 |
CA2981992C (en) * | 2007-03-20 | 2020-06-30 | Becton, Dickinson And Company | Assays using surface-enhanced raman spectroscopy (sers)-active particles |
GB2457094A (en) * | 2008-02-04 | 2009-08-05 | Univ Dublin City | A cuvette assembly for holding milking samples |
MX2011000794A (es) | 2008-07-21 | 2011-02-24 | Becton Dickinson Co | Dispositivo de separacion de fase por densidad. |
US8309343B2 (en) | 2008-12-01 | 2012-11-13 | Baxter International Inc. | Apparatus and method for processing biological material |
EP4119228A1 (en) | 2009-05-15 | 2023-01-18 | Becton, Dickinson and Company | Density phase separation device |
JP5763894B2 (ja) * | 2009-07-01 | 2015-08-12 | タカラバイオ株式会社 | 細胞の分離方法 |
JP5265815B2 (ja) * | 2010-08-20 | 2013-08-14 | 国立大学法人北陸先端科学技術大学院大学 | 血球の密度変化を利用した密度勾配遠心による有核赤血球の回収法 |
JP6057251B2 (ja) | 2011-11-11 | 2017-01-11 | 国立研究開発法人産業技術総合研究所 | 粒子分別装置および粒子分別方法 |
US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
US11291931B2 (en) | 2014-12-15 | 2022-04-05 | Akadeum Life Sciences, Inc. | Method and system for buoyant separation |
US20180120295A1 (en) * | 2015-05-01 | 2018-05-03 | Mesotex, Inc. | Process for separating nucleated cells from non-nucleated red blood cells |
JP2017055759A (ja) * | 2015-09-18 | 2017-03-23 | 東ソー株式会社 | 比重分離を利用した細胞の分離回収方法 |
WO2017065820A1 (en) * | 2015-10-14 | 2017-04-20 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
CA3099911A1 (en) | 2018-07-09 | 2020-01-16 | Hanuman Pelican, Inc. | Apparatus and methods for processing blood |
CN112437692B (zh) | 2018-07-09 | 2022-06-24 | 爱卡德姆生命科学公司 | 用于有浮力颗粒处理的系统和方法 |
KR102536272B1 (ko) * | 2021-01-29 | 2023-05-24 | 주식회사 큐리오시스 | 용액분리기를 포함하는 원심분리 튜브 및 이를 이용한 혼합 용액의 분리 방법 |
WO2023028329A1 (en) | 2021-08-26 | 2023-03-02 | Akadeum Life Sciences, Inc. | Method and system for buoyant separation |
WO2023200777A1 (en) * | 2022-04-12 | 2023-10-19 | Massachusetts Institute Of Technology | Device and method for isolation and detection of targets in a sample |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2115032C3 (de) * | 1971-03-29 | 1975-05-07 | Kernforschungsanlage Juelich Gmbh, 5170 Juelich | Vorrichtung zum Trennen zweier Phasen bei der Flüssig-Flüssig-Extraktion in einer Zentrifuge |
IL74967A (en) * | 1985-04-18 | 1988-10-31 | Assaf Pharmaceutical Ind | Separation of materials from a liquid dispersion by sedimentation |
US4927750A (en) * | 1986-04-09 | 1990-05-22 | Jeanette Simpson | Cell separation process |
IL100828A (en) * | 1992-01-31 | 2002-05-23 | Novamed Ltd | Method and means for density gradient centrifugation |
US5457024A (en) * | 1993-01-22 | 1995-10-10 | Aprogenex, Inc. | Isolation of fetal erythrocytes |
-
1995
- 1995-08-31 ES ES95931685T patent/ES2140705T3/es not_active Expired - Lifetime
- 1995-08-31 NZ NZ292756A patent/NZ292756A/xx not_active IP Right Cessation
- 1995-08-31 DE DE69513188T patent/DE69513188T2/de not_active Expired - Lifetime
- 1995-08-31 CA CA002198607A patent/CA2198607C/en not_active Expired - Lifetime
- 1995-08-31 AU AU35025/95A patent/AU700743B2/en not_active Expired
- 1995-08-31 EP EP95931685A patent/EP0778944B1/en not_active Expired - Lifetime
- 1995-08-31 DK DK95931685T patent/DK0778944T3/da active
- 1995-08-31 WO PCT/US1995/011169 patent/WO1996007097A1/en active IP Right Grant
- 1995-08-31 JP JP50898596A patent/JP3397795B2/ja not_active Expired - Lifetime
- 1995-08-31 AT AT95931685T patent/ATE186398T1/de active
-
1998
- 1998-12-23 HK HK98115108A patent/HK1013862A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
AU700743B2 (en) | 1999-01-14 |
DE69513188D1 (de) | 1999-12-09 |
HK1013862A1 (en) | 1999-09-10 |
CA2198607C (en) | 2000-04-18 |
WO1996007097A1 (en) | 1996-03-07 |
EP0778944B1 (en) | 1999-11-03 |
JPH10508190A (ja) | 1998-08-18 |
AU3502595A (en) | 1996-03-22 |
JP3397795B2 (ja) | 2003-04-21 |
EP0778944A1 (en) | 1997-06-18 |
ATE186398T1 (de) | 1999-11-15 |
CA2198607A1 (en) | 1996-03-07 |
DK0778944T3 (da) | 2000-05-01 |
DE69513188T2 (de) | 2000-07-06 |
NZ292756A (en) | 1998-10-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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