WO1996007097B1 - Cell separation apparatus and method - Google Patents
Cell separation apparatus and methodInfo
- Publication number
- WO1996007097B1 WO1996007097B1 PCT/US1995/011169 US9511169W WO9607097B1 WO 1996007097 B1 WO1996007097 B1 WO 1996007097B1 US 9511169 W US9511169 W US 9511169W WO 9607097 B1 WO9607097 B1 WO 9607097B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tube
- constriction member
- constriction
- centrifugation
- cell
- Prior art date
Links
- 238000000926 separation method Methods 0.000 title claims abstract 10
- 238000005119 centrifugation Methods 0.000 claims 6
- 239000012530 fluid Substances 0.000 claims 3
- 210000001736 Capillaries Anatomy 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 210000004027 cells Anatomy 0.000 abstract 7
- 208000000409 Breast Neoplasms Diseases 0.000 abstract 1
- 206010006187 Breast cancer Diseases 0.000 abstract 1
- 102100016492 CD34 Human genes 0.000 abstract 1
- 108060001251 CD34 Proteins 0.000 abstract 1
- 210000003958 Hematopoietic Stem Cells Anatomy 0.000 abstract 1
- 230000001605 fetal Effects 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000001225 therapeutic Effects 0.000 abstract 1
- 210000004881 tumor cells Anatomy 0.000 abstract 1
Abstract
Disclosed are methods for enriching specific cell types from cell mixtures. Included in the methods are the use of precisely defined cell separation media, a cell separation apparatus that includes a constriction defining a lower region and a defined cell separation medium. The constriction prevents mixing between the upper and lower portions of the tube. Also disclosed is a density adjusted cell separation technique used to augment the above methods. In addition, the invention includes specific methods for isolating CD34+ hematopoietic progenitor cells, nucleated fetal cells, and breast tumor cells. The isolation methods are useful in various diagnostic and therapeutic regimens.
Claims
1. A cell-separation apparatus, comprising: a centrifugation tube having side walls and a closed bottom; a constriction member disposed within the tube, said constriction member posi¬ tioned and constructed to retain fluid in the bottom portion of the tube below the constriction member, when the tube is inverted, and a cell-separation medium contained in the bottom portion of the tube and, prior to centrifugation of said tube, extending above said constriction member to a level above an opening formed by said constriction member, such that cells which are captured at an interface between the cell-separation medium and a lower-density medium, after centrifugation, are discharged with the lower-density medium when the tube is inverted.
2. The apparatus of claim 1, wherein said constriction member defines one or more downwardly sloped surfaces having lower edge regions defining a constricted opening.
3. The apparatus of claim 1, wherein said constriction element is an annulus having a central constricted opening.
4. The apparatus of claim 3, wherein said annulus is an elastomeric annulus constructed for forced fit into the tube at a selected tube level.
5. The apparatus of claim 1, wherein said constriction member defines a plurality of openings.
6. The apparatus of claim 1, wherein said tube includes a closed top, and a port in said top through which fluid material can be introduced into the tube.
7. The apparatus of claim 6, which further includes a second port in said top and a closed fluid channel communicating the second port with the bottom of the tube below said constriction member.
STATEMENT UNDER ARTICLE 19
Claim l has been amended to bring out the feature that the cell separation medium contained within the tube extends above the constriction member in the tube prior to centrifugation of the tube. This contrasts with the disclosures of EP,A,0 198,462 (Assaf Pharmaceutical Ind.) and of US,A,5 314 074 (Inbar Michael, et al . ) in which separation medium is initially present only up to the level of the constriction (top of capillary bore in the Assaf reference or beneath the insert in the Inbar reference) . In the cited references, the centrifugation medium may rise above the level of the constriction member only after centrifugation of the tube and its contents.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP50898596A JP3397795B2 (en) | 1994-08-31 | 1995-08-31 | Cell separation device and method |
| EP95931685A EP0778944B1 (en) | 1994-08-31 | 1995-08-31 | Cell separation apparatus and method |
| NZ292756A NZ292756A (en) | 1994-08-31 | 1995-08-31 | Cell separation apparatus and method using centrifugation and density mediums |
| DK95931685T DK0778944T3 (en) | 1994-08-31 | 1995-08-31 | Cell separation apparatus and method |
| CA002198607A CA2198607C (en) | 1994-08-31 | 1995-08-31 | Cell separation apparatus and method |
| AU35025/95A AU700743B2 (en) | 1994-08-31 | 1995-08-31 | Cell separation apparatus and method |
| DE69513188T DE69513188T2 (en) | 1994-08-31 | 1995-08-31 | DEVICE AND METHOD FOR SEPARATING CELLS |
| HK98115108A HK1013862A1 (en) | 1994-08-31 | 1998-12-23 | Cell separation apparatus and method |
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/299,468 | 1994-08-31 | ||
| US08/299,469 US5474687A (en) | 1994-08-31 | 1994-08-31 | Methods for enriching CD34+ human hematopoietic progenitor cells |
| US08/299,465 US5648223A (en) | 1994-08-31 | 1994-08-31 | Methods for enriching breast tumor cells |
| US08/299,468 US5646004A (en) | 1994-08-31 | 1994-08-31 | Methods for enriching fetal cells from maternal body fluids |
| US08/299,465 | 1994-08-31 | ||
| US08/299,469 | 1994-08-31 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1996007097A1 WO1996007097A1 (en) | 1996-03-07 |
| WO1996007097B1 true WO1996007097B1 (en) | 1996-05-02 |
Family
ID=27404658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1995/011169 WO1996007097A1 (en) | 1994-08-31 | 1995-08-31 | Cell separation apparatus and method |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0778944B1 (en) |
| JP (1) | JP3397795B2 (en) |
| AT (1) | ATE186398T1 (en) |
| AU (1) | AU700743B2 (en) |
| CA (1) | CA2198607C (en) |
| DE (1) | DE69513188T2 (en) |
| DK (1) | DK0778944T3 (en) |
| ES (1) | ES2140705T3 (en) |
| HK (1) | HK1013862A1 (en) |
| NZ (1) | NZ292756A (en) |
| WO (1) | WO1996007097A1 (en) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5577513A (en) * | 1994-08-31 | 1996-11-26 | Activated Cell Therapy, Inc. | Centrifugation syringe, system and method |
| GB9422504D0 (en) | 1994-11-08 | 1995-01-04 | Robertson Patricia M B | Blood testing |
| DE19681033D2 (en) | 1995-11-16 | 1999-03-18 | Michael W Dahm | Method for the quantification of tumor cells in a body fluid and suitable test kits |
| WO1997021488A1 (en) * | 1995-12-11 | 1997-06-19 | Dendreon Corporation | Cell separation composition, kit and method |
| CA2280129C (en) | 1997-02-14 | 2006-10-24 | Dendreon Corporation | Cell washing device and method |
| EP0973873B1 (en) * | 1997-04-08 | 2006-06-07 | Pall Corporation | Method of harvesting rare cells from blood products |
| IT1295939B1 (en) | 1997-10-31 | 1999-05-28 | Giammaria Sitar | DEVICE AND METHOD FOR THE SEPARATION OF HUMAN OR ANIMAL CELLS WITH DIFFERENT DENSITIES FROM CELL DISPERSIONS THAT CONTAIN THEM |
| AU9233698A (en) * | 1997-11-22 | 1999-06-17 | Robert A. Levine | Method for the detection, identification, enumeration and confirmation of circulating cancer cells and/or hematologic progenitor cells in whole blood |
| DE19804372A1 (en) | 1998-02-04 | 1999-08-05 | Michael W Dr Dr Dahm | Method for the quantitative determination of tumor cells in a body fluid and suitable test kits |
| DE19904267A1 (en) * | 1999-02-03 | 2000-08-10 | Michael W Dahm | Method for the enrichment of tumor cells from a body fluid and a suitable kit for this |
| US7030228B1 (en) | 1999-11-15 | 2006-04-18 | Miltenyi Biotec Gmbh | Antigen-binding fragments specific for dendritic cells, compositions and methods of use thereof antigens recognized thereby and cells obtained thereby |
| US7947236B2 (en) | 1999-12-03 | 2011-05-24 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
| EP1462800A4 (en) * | 2001-12-11 | 2007-01-17 | Netech Inc | Blood cell separation system |
| WO2005030361A1 (en) * | 2003-09-30 | 2005-04-07 | Keith Richard Mitchelson | Apparatus and method for centrifugal separation |
| ITRM20030467A1 (en) * | 2003-10-10 | 2005-04-11 | Advance Holdings Ltd | DISPOSABLE CONTAINER FOR CENTRIFUGATION AND THE TREATMENT OF A FLUID BIOLOGICAL MATERIAL. |
| CA2629867C (en) | 2005-11-14 | 2015-12-29 | Rijk Zwaan Zaadteelt En Zaadhandel B.V. | Method for removing a fluid from a container and device |
| JP2008220319A (en) * | 2007-03-15 | 2008-09-25 | Kaneka Corp | Vessel for centrifugal separation, and supporting instrument for the vessel |
| EP2461163A3 (en) * | 2007-03-20 | 2012-09-26 | Becton, Dickinson and Company | Assays using surface-enhanced raman spectroscopy (sers)-active particles |
| GB2457094A (en) * | 2008-02-04 | 2009-08-05 | Univ Dublin City | A cuvette assembly for holding milking samples |
| CN104588140B (en) | 2008-07-21 | 2016-06-29 | 贝克顿·迪金森公司 | Density phase separation device |
| US8309343B2 (en) | 2008-12-01 | 2012-11-13 | Baxter International Inc. | Apparatus and method for processing biological material |
| BR122021008555B1 (en) | 2009-05-15 | 2022-03-03 | Becton, Dickinson And Company | SEPARATION SET FOR SEPARATING A FLUID SAMPLE IN FIRST PHASE AND SECOND PHASE |
| JP5763894B2 (en) * | 2009-07-01 | 2015-08-12 | タカラバイオ株式会社 | Cell separation method |
| JP5265815B2 (en) * | 2010-08-20 | 2013-08-14 | 国立大学法人北陸先端科学技術大学院大学 | Recovery of nucleated red blood cells by density gradient centrifugation using changes in blood cell density |
| JP6057251B2 (en) | 2011-11-11 | 2017-01-11 | 国立研究開発法人産業技術総合研究所 | Particle sorting apparatus and particle sorting method |
| US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
| US11291931B2 (en) * | 2014-12-15 | 2022-04-05 | Akadeum Life Sciences, Inc. | Method and system for buoyant separation |
| US20180120295A1 (en) * | 2015-05-01 | 2018-05-03 | Mesotex, Inc. | Process for separating nucleated cells from non-nucleated red blood cells |
| JP2017055759A (en) * | 2015-09-18 | 2017-03-23 | 東ソー株式会社 | Methods for isolating and recovering cells using specific gravity separation |
| WO2017065820A1 (en) * | 2015-10-14 | 2017-04-20 | Rarecyte, Inc. | Apparatus, system, and method for collecting a target material |
| EP3820541A4 (en) * | 2018-07-09 | 2022-04-06 | Hanuman Pelican, Inc. | Apparatus and methods for processing blood |
| WO2020014273A1 (en) | 2018-07-09 | 2020-01-16 | Akadeum Life Sciences, Inc. | System and method for buoyant particle processing |
| KR102536272B1 (en) * | 2021-01-29 | 2023-05-24 | 주식회사 큐리오시스 | Centrifuge tube comprising fluid divider and method of separating mixed solution using the same |
| WO2023028329A1 (en) | 2021-08-26 | 2023-03-02 | Akadeum Life Sciences, Inc. | Method and system for buoyant separation |
| WO2023200777A1 (en) * | 2022-04-12 | 2023-10-19 | Massachusetts Institute Of Technology | Device and method for isolation and detection of targets in a sample |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2115032C3 (en) * | 1971-03-29 | 1975-05-07 | Kernforschungsanlage Juelich Gmbh, 5170 Juelich | Device for separating two phases in liquid-liquid extraction in a centrifuge |
| IL74967A (en) * | 1985-04-18 | 1988-10-31 | Assaf Pharmaceutical Ind | Separation of materials from a liquid dispersion by sedimentation |
| US4927750A (en) * | 1986-04-09 | 1990-05-22 | Jeanette Simpson | Cell separation process |
| IL100828A (en) * | 1992-01-31 | 2002-05-23 | Novamed Ltd | Method and means for density gradient centrifugation |
| US5457024A (en) * | 1993-01-22 | 1995-10-10 | Aprogenex, Inc. | Isolation of fetal erythrocytes |
-
1995
- 1995-08-31 NZ NZ292756A patent/NZ292756A/en not_active IP Right Cessation
- 1995-08-31 EP EP95931685A patent/EP0778944B1/en not_active Expired - Lifetime
- 1995-08-31 JP JP50898596A patent/JP3397795B2/en not_active Expired - Lifetime
- 1995-08-31 DE DE69513188T patent/DE69513188T2/en not_active Expired - Lifetime
- 1995-08-31 AT AT95931685T patent/ATE186398T1/en active
- 1995-08-31 DK DK95931685T patent/DK0778944T3/en active
- 1995-08-31 WO PCT/US1995/011169 patent/WO1996007097A1/en active IP Right Grant
- 1995-08-31 ES ES95931685T patent/ES2140705T3/en not_active Expired - Lifetime
- 1995-08-31 CA CA002198607A patent/CA2198607C/en not_active Expired - Lifetime
- 1995-08-31 AU AU35025/95A patent/AU700743B2/en not_active Expired
-
1998
- 1998-12-23 HK HK98115108A patent/HK1013862A1/en not_active IP Right Cessation
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